• Journal of Microbiology and Biotechnology
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• v.20 no.7
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• pp.1156-1162
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• 2010

During ethanol fermentation, bacterial strains may encounter various stresses, such as ethanol and acid shock, which adversely affect cell viability and the production of ethanol. Therefore, ethanologenic strains that tolerate abiotic stresses are highly desirable. Bacteria of the genus Deinococcus are extremely resistant to ionizing radiation, ultraviolet light, and desiccation, and therefore constitute an important pool of extreme resistance genes. The irrE gene encodes a general switch responsible for the extreme radioresistance of D. radiodurans. Here, we present evidence that IrrE, acting as a global regulator, confers high stress tolerance to a Zymomonas mobilis strain. Expression of the gene protected Z. mobilis cells against ethanol, acid, osmotic, and thermal shocks. It also markedly improved cell viability, the expression levels and enzyme activities of pyruvate decarboxylase and alcohol dehydrogenase, and the production of ethanol under both ethanol and acid stresses. These data suggest that irrE is a potentially promising gene for improving the abiotic stress tolerance of ethanologenic bacterial strains.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.13 no.12
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• pp.1058-1062
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• 2000

We investigated resistive superconducting fault current limites (SFCLs) fabricated using YBCO thin films on 2-inch diameter sapphire substrates. Nearly identical SFCL units were prepared and tested. The units were connected in series and parallel to increase the current and voltage ratings. A serial connection of the units showed significantly unbalanced power dissipation between the units. This imbalance was removed by introducing a shunt resistor to the firstly quenched unit. Parallel connection of the units increased the current rating. An SFCL module of 4 units in parallel, each of which has minimum quench current rating. An SFCL module of 4 units in parallel, each of which has minimum quench current 25 A$\_$peak/, was produced and successfully tested at a 220 V$\_$rms/circuit. From the resistance increase, we estimated that the film temperature increased to 200 K in 5 msec, and 300 K in 120 msec. Successive quenches revealed that this system is stable without degradation in the current limiting capability under such thermal shocks as quenches at 220 V$\_$rms/.

• Fisheries and Aquatic Sciences
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• v.4 no.1
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• pp.39-46
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• 2001

To examine the effect of copy number-dependent transgenic genotype on the expression of foreign gene, stable hemizygous and homozygous transgenic breeding line was established using artificial parthenogenesis. For this purpose, induced diploid gynogenetic transgenesis was optimized in mud loach (Misgurnus mizolepis) using UV-irradiated cyprinid loach (M. anguillicaudatus) sperm and thermal shocks. Optimum UV range for inactivation of cyprinid loach sperm was between 3,150 to $4,050\;ergs/mm^2$ The UV-irradiated sperm were inseminated into eggs from recessive color strain (yellow) or heterozygous transgenic mud loach containing CAT gene. Cold shock at $2^{\circ}C$ for 60 min, 5 min post fertilization successfully restored the diploidy of eggs inseminated with UV-irradiated sperm. Restoration to diploidy was confirmed by flow cytometry and gynogenetic status was verified by examining maternal exclusive inheritance of multi-locus DNA fingerprints, body color and transgenic marker. Putative isogenic transgenic fish clearly showed homozygous status at trans gene locus based on Southern blot hybridization and progeny testing. Further, such homozygous gynogenetic diploids revealed the increased levels of transgene expression, when compared to those of heterozygous (hemizygous) transgenic fish.

• 한국초전도학회:학술대회논문집
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• v.10
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• pp.272-275
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• 2000

We investigated a resistive superconducting fault current limiter (SFCL) fabricated using YBCO thin films on 2-inch diameter sapphire substrates. Nearly identical SFCL units were prepared and tested. The units were connected in series and parallel to increase the current and voltage ratings. A serial connection of the units showed significantly unbalanced power dissipation between the units. This imbalance was removed by introducing a shunt resistor to the firstly quenched unit. Parallel connection of the units increased the current rating. An SFCL module of 4 units in parallel, each of which has minimum quench current 25 Ap, was produced and successfully tested at a 220 V circuit. From the resistance increase, we estimated that the film temperature increases to 200 K in 5 msec, and 300 K in 120 msec. Successive quenches revealed that this system is stable without degradation in the current limiting capability under such thermal shocks as quenches at 220 V.

• The Bulletin of The Korean Astronomical Society
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• v.35 no.1
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• pp.69.1-69.1
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• 2010

IC 443 is famous for its interaction with nearby molecular clouds and intense H2 emission lines in infrared. Therefore, it has been studied extensively for the understanding of molecular shocks. We observed H2 mission lines toward the shock-cloud interaction regions of IC 443, known as clumps B, C, and G. The observations were performed with the InfraRed Camera (IRC) onboard a satellite AKARI over 2.5-5.0 um, where previous space observations, e.g. Infrared Space Observatory (ISO) and Spitzer, do not cover. Our AKARI observations provide spectra of sequential pure-rotational and ro-vibrational H2 emission lines. For the clumps C and G, combining with previous mid-infrared observational results, we found that the H2 level populations show a significant separation between v=0 and v=1 levels; v=1 levels are under-populated than v=0 levels, therefore, the population cannot be described by two temperature LTE model, as many people have analyzed for the shocked H2 gas. We also applied the thermal admixture model, dN(H2; T)~T^(-b) dT, with varying ortho-to-para ratios according to the temperature, to describe the level population, and obtained plausible ranges of the H2 gas density and power-law index b.

• Publications of The Korean Astronomical Society
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• v.30 no.2
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• pp.433-437
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• 2015

We show the results of a time series analysis of the long-term light curves of four blazars. 3C 279, 3C 345, 3C 446, and BL Lacertae. We used densely sampled light curves spanning 32 years at three frequency bands (4.8, 8, 14.5 GHz), provided by the University of Michigan Radio Astronomy Observatory monitoring program. The spectral indices of our sources are mostly flat or inverted (-0.5 < ${\alpha}$ < 0), which is consistent with optically thick emission. Strong variability was seen in all light curves on various time scales. From the analyses of time lags between the light curves from different frequency bands and the evolution of the spectral indices with time, we find that we can distinguish high-peaking flares and low-peaking flares according to the Valtaoja et al. classification. The periodograms (temporal power spectra) of the light curves are in good agreement with random-walk power-law noise without any indication of (quasi-)periodic variability. We note that random-walk noise light curves can originate from multiple shocks in jets. The fact that all our sources are in agreement with being random-walk noise emitters at radio wavelengths suggests that such behavior is a general property of blazars. We are going to generalize our approach by applying our methodology to a much larger blazar sample in the near future.

• JSTS:Journal of Semiconductor Technology and Science
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• v.14 no.4
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• pp.495-502
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• 2014

Fast recovery diodes (FRDs) were developed using the $p^{{+}{+}}/n^-/n^{{+}{+}}$ epitaxial layers grown by low temperature epitaxy technology. We investigated the effect of electrostatic discharge (ESD) stresses on their electrical and switching properties using current-voltage (I-V) and reverse recovery time analyses. The FRDs presented a high breakdown voltage, >450 V, and a low reverse leakage current, < $10^{-9}$ A. From the temperature dependence of thermal activation energy, the reverse leakage current was dominated by thermal generation-recombination and diffusion, respectively, at low and high temperature regions. By virtue of the abrupt junction and the Pt drive-in for the controlling of carrier lifetime, the soft reverse recovery behavior could be obtained along with a well-controlled reverse recovery time of 21.12 ns. The FRDs exhibited excellent ESD robustness with negligible degradations in the I-V and the reverse recovery characteristics up to ${\pm}5.5$ kV of HBM and ${\pm}3.5$ kV of IEC61000-4-2 shocks. Likewise, transmission line pulse (TLP) analysis reveals that the FRDs can handle the maximum peak pulse current, $I_{pp,max}$, up to 30 A in the forward mode and down to - 24 A in the reverse mode. The robust ESD property can improve the long term reliability of various power applications such as automobile and switching mode power supply.

• Journal of The Korean Astronomical Society
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• v.33 no.2
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• pp.111-121
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• 2000

Many models of globular cluster formation assume the presence of cold dense clouds in early universe. Here we re-examine the Fall & Rees (1985) model for formation of proto-globular cluster clouds (PGCCs) via thermal instabilities in a protogalactic halo. We first argue, based on the previous study of two-dimensional numerical simulations of thermally unstable clouds in a stratified halo of galaxy clusters by Real et al. (1991), that under the protogalactic environments only nonlinear (${\delta}{\ge}1$) density inhomogeneities can condense into PGCCs without being disrupted by the buoyancy-driven dynamical instabilities. We then carry out numerical simulations of the collapse of overdense douds in one-dimensional spherical geometry, including self-gravity and radiative cooling down to T = $10^4$ K. Since imprinting of Jeans mass at $10^4$ K is essential to this model, here we focus on the cases where external UV background radiation prevents the formation of $H_2$ molecules and so prevent the cloud from cooling below $10^4$ K. The quantitative results from these simulations can be summarized as follows: 1) Perturbations smaller than $M_{min}\~(10^{5.6}\;M{\bigodot})(nh/0.05cm^{-3})^{-2}$ cool isobarically, where nh is the unperturbed halo density, while perturbations larger than $M_{min}\~(10^8\;M{\bigodot})(nh/0.05cm^{-3})^{-2}$ cool isochorically and thermal instabilities do not operate. On the other hand, intermediate size perturbations ($M_{min} < M_{pgcc} < M_{max}$) are compressed supersonically, accompanied by strong accretion shocks. 2) For supersonically collapsing clouds, the density compression factor after they cool to $T_c = 10^4$ K range $10^{2.5} - 10^6$, while the isobaric compression factor is only $10^{2.5}$. 3) Isobarically collapsed clouds ($M < M_{min}$) are too small to be gravitationally bound. For supersonically collapsing clouds, however, the Jeans mass can be reduced to as small as $10^{5.5}\;M_{\bigodot}(nh/0.05cm^{-3})^{-1/2}$ at the maximum compression owing to the increased density compression. 4) The density profile of simulated PGCCs can be approximated by a constant core with a halo of $p{\infty} r^{-2}$ rather than a singular isothermal sphere.

• Development and Reproduction
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• v.6 no.2
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• pp.71-76
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• 2002

Present study was aimed to summary the recent reports of chromosomal technology such like a polyploidv, sex differentiation, gynogenesis, transgenic fish and gene manipulation. Triploid cells for rainbow trout and channel catfish were induced through thermal shocks of varying temperature levels and produced as a industrial use. A monosex fish with homogametic females of 15 species of high valued fish were produced by exposing to irradiation. It seemed that different irradiation was suitable to inactivate the sperm and block the formation in producing the gynogenetic diploids. Since 1985, transgenic fish have been successfully produced by microinjecting or electroporating desired foreign DNA into unfertilized or newly fertilized eggs using about 40 fish species. More recently, transgenic fish have also been produced by infecting newly fertilized eggs with pantropic, defective retroviral vectors carrying desired foreign DNA. These transgenic fish can serve as excellent experimental models for basic scientific investigations as well as in marine biotechnological applications.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.1
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• pp.82.2-82.2
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• 2012

We present Herschel-PACS observations of L1448-MM, a Class 0 protostar with a prominent outflow, part of the DIGIT Key Program (PI: N. Evans). We detect numerous emission lines including CO and $H_2O$ rotational transitions, OH transitions, and [OI] forbidden transitions at wavelengths from 55 to 210 ${\mu}m$. The $H_2O$, [OI], mid-J CO (J < 23), and OH emission distributes along the outflow direction although high-J CO and other OH emission peaks at the central spatial pixel. According to our simple excitation analysis, CO seems to have two temperature components of warm and hot, which might be attributed to the PDR and shock, respectively. After exploring a wide range of physical conditions with a non-LTE LVG code, RADEX, we found that either shock alone or the combination of PDR and shock can explain the observations. The relative fraction of observed line luminosities suggest that L1448-MM is shielded from the UV radiation because $H_2O$ and CO are the dominant coolants rather than OH and [OI]. In addition, our observed fluxes match better with C-shock models rather than J-shocks. The non-LTE LVG model supports that the IR pumping process is important for OH transitions because the OH line ratios are fitted much better when the dust thermal continuum is included.