• International Journal of Oral Biology
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• v.34 no.1
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• pp.37-42
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• 2009

The attachment and adhesion of RAW 264.7 and MC3T3-E1 cells to titanium (Ti) discs with various degrees of roughness was investigated. The attachment, adhesion, and proliferation of these cells were evaluated after 4 hr, 24 hr and 7 day incubations. Both RAW 264.7 and MC3T3-E1 cells showed a time-dependant correlation between attachment and adhesion on the surface of the titanium discs. Both types of cells tended to have higher survival rate on these discs as the surface roughness increased. The percentage of adherent inflammatory RAW 264.7 cells was greater than MC3T3-E1 cells at 24 hr, but this was reversed at 7 days in culture. The morphology of osteoblastic MC3T3-E1 cells at 24 hr, determined using a surface emission microscope (SEM), appeared flattened and spread out while inflammatory RAW 264.7 cells were predominantly spherical in shape. The adhesion of both cell types on the titanium discs was dependant on the levels of fibronectin adsorbed on the disc surface, indicating that serum constituents modulate the efficient adhesion of these cells. Our data indicate that the cellular response to the titanium surface is dependent on the types of cells, surface roughness and serum constituents.

• 대한임상약리학회:학술대회논문집
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• 2002.05a
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• pp.13-15
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• 2002

• 대한약리학회:학술대회논문집
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• 2006.11a
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• pp.44-44
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• 2006

• 대한임상약리학회:학술대회논문집
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• 2006.11a
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• pp.139-140
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• 2006

• 한국당과학회:학술대회논문집
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• 2006.11a
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• pp.21-22
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• 2006

• 대한임상약리학회:학술대회논문집
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• 2001.06a
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• pp.16-17
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• 2001

• International Journal of Oral Biology
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• v.44 no.4
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• pp.173-181
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• 2019

The CC chemokine receptor 5 (CCR5) is a G protein-coupled receptor that regulates chemotaxis and effector functions of immune cells. It also serves as the major co-receptor for the entry of human immunodeficiency virus (HIV). Recently, CCR5 inhibitors have been developed and used for the treatment or prevention of HIV infections. Additionally, it has been identified that CCR5 controls bone homeostasis by regulating osteoclastogenesis and the communication between osteoblasts and osteoclasts. However, the effects of CCR5 inhibition on bone tissue in elderly patients are unknown. This study aimed to examine the bone phenotype of aged CCR5 knockout (KO) mice. Femoral and tibial bones were isolated from 12-month and 18-month old wild-type (WT) and CCR5 KO mice, and microcomputed tomography and histology analyses were performed. Twelve-month-old CCR5 KO mice exhibited a decreased trabecular bone mass and cortical bone thickness in both femoral and tibial bones compared with age-matched WT mice. Eighteen-month-old mice also showed a decreased trabecular bone mass in femurs compared with control WT mice, but not in tibial bones. Unlike in 12-month-old mice, the cortical margin of femurs and tibias in 18-month-old mice were rough, likely because they were aggravated by the deficiency of CCR5. Overall, our data suggest that the deficiency of CCR5 with aging can cause severe bone loss. When CCR5 inhibitors or CCR5 inactivating technologies are used in elderly patients, a preventive strategy for bone loss should be considered.

• Phonetics and Speech Sciences
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• v.14 no.4
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• pp.57-66
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• 2022

In this study, the definition of "digital therapeutics" was clarified by examining related studies, and the development trend of digital therapeutics at the domestic level was investigated. Further, research data and technologies applied to various communication disorders since 2015 were analyzed. From all these, digital therapeutics can be defined as software that can support evidence-based treatment when used on patients to prevent, manage, and treat disorders With huge investments and research efforts increasingly made in the field of digital therapeutics, 17 of the 22 studies examined were on digital therapeutics applied in the treatment of language disorders. In the research papers examined, the technologies applied were virtual reality and augmented reality, with augmented reality used in most cases. The effects of applying digital treatment were positive, and most studies focused on content development in relation to the development of digital treatment, although one study was conducted for app development. Future studies could examine the application of digital therapeutics to more diverse communication disorder subjects. Active government support is expected in developing more sophisticated software that can be applied using a wider range of technologies in the field of digital therapeutics to treat more communication disorders.

• Journal of Acupuncture Research
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• v.29 no.6
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• pp.91-104
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• 2012

Objectives : The purpose of this study is to observe the effect of Intranasal Bloodletting Therapeutics on the Allergic Rhinitis. Methods : 8 patients were treated by intranasal bloodletting therapeutics to recover from allergic rhinitis. The symtoms were evaluated by total nasal sympton score(TNSS), verbal numerical rating scale(VNRS). Results : Total nasal sympton score(TNSS) was significantly improved and verbal numerical rating scale(VNRS) was decreased in all cases. Conclusion : Intranasal bloodletting therapeutics is effective to allergic Rhinitis.

• International Journal of Oral Biology
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• v.44 no.4
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• pp.182-190
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• 2019

Periodontitis is an inflammatory disease of the supportive tissues surrounding the teeth, and is characterized by irreversible destruction of the gingiva, periodontal ligament (PDL), and alveolar bone, which results in the loss of teeth. In the present study, we elucidated the correlation between periodontitis and apelin (APLN), an adipokine and a regulatory peptide, respectively, which are involved in inflammation and bone remodeling. The expression of APLN is negatively correlated with periodontitis progression in gingival tissue. In addition, treatment with TNF-α downregulated the expression of APLN in PDL cells and gingival fibroblasts, indicating the protective role played by APLN against periodontitis progression. The overexpression of APLN or treatment with exogenous APLN suppressed the TNF-α-mediated catabolic gene expression of MMP1, IL6, and PTGS2 in PDL cells. Moreover, the inhibition of the APLNA-PJ axis by ML221, an APJ inhibitor, induced catabolic gene expression in PDL cells. Thus, the results of this study provided evidence to support APLN as a regulatory factor of the inflammatory response during periodontitis.