• Korean Journal of Acupuncture
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• 제38권2호
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• pp.62-74
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• 2021

Objectives : The aim of this study is to analyze the effect of acupuncture and the details of acupuncture treatment methods on skin wrinkles. Methods : Search was conducted in Pubmed, KISS, and NDSL databases for acupuncture studies on skin wrinkles. The detailed therapeutic techniques of acupuncture used for skin wrinkles and the effect of acupuncture on skin wrinkle improvement were analyzed. Then, the study results using the same indicator were compared through meta-analysis in order to compare the effects of acupuncture with the control group. The quality of randomized controlled trials (RCTs) was assessed using the risk of bias (ROB) assessment tool (Ver. 1.0, Cochrane Collaboration). Results : A total of 10 RCTs and 19 case series were included in this study. The most frequently used therapeutic technique for skin wrinkle improvement was Microneedle therapy system (MTS, n=19), and they were mostly used with aesthetic solutions (13 out of 19 studies). Skin wrinkle related indexes were most commonly used to evaluate skin wrinkle improvement (n=14). Panax Ginseng pharmacopuncture showed the most remarkable effect in improving the range of skin wrinkles and the depth of skin wrinkles. MTS+epidermal growth factor improved the levels of skin hydration, skin elasticity, and variation of skin pigment the most. MTS+Endothelial precursor cell improved the number of skin melanin the most. Three RCTs included in the meta-analysis showed moderate ROB on average. Conclusions : Acupuncture treatment was effective in improving skin wrinkles. However, a precise study design using unified indicators and appropriate controls needs to be performed in further studies in order to establish more convincing evidence of the effectiveness of acupuncture for improving skin wrinkles.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제24권4호
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• pp.384-391
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• 2021

Purpose: The Cow's Milk-related Symptom Score (CoMiSS^TM) was developed as an awareness tool for evaluating cow's milk-related symptoms in otherwise healthy children. Using a convenience sample of participants, this cross-sectional study aimed to determine CoMiSS^TM values of symptomatic infants based on retrospectively or prospectively obtained information. Methods: CoMiSS^TM values were determined in infants aged <12 months with symptoms suggestive of cow's milk protein allergy or functional gastrointestinal disorders. The exclusion criteria were previous diagnosis with acute or chronic disease, treatment with a therapeutic formula, and in case of breastfeeding, an elimination diet followed by the mother. Two CoMiSS^TM values were assessed. A retrospective collection was defined as the collection of data after initial contact with the medical center but before the first medical consultation. A prospective collection was defined as the collection of data within 24 hours from the time of medical consultation but before starting any therapeutic intervention. The CoMiSS^TM total and individual component scores obtained retrospectively or prospectively were compared between groups using the Wilcoxon signed-rank test. Results: This study was performed between August and November 2019. Data of 110 children (62 males and 48 females), with a mean±standard deviation age of 18.2±11.7 weeks, were obtained. The total CoMiSS^TM value (p<0.001) and some individual component scores (crying, regurgitation, and stool) were significantly lower when collected prospectively than when collected retrospectively. Conclusion: CoMiSS^TM values were retrospectively and prospectively determined. Lower CoMiSS^TM values were obtained during prospective evaluation. Possible differences should be considered when using CoMiSS^TM in clinical practice.

• Journal of Acupuncture Research
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• 제39권4호
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• pp.283-296
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• 2022

In this study, the evidence of thread embedding acupuncture (TEA) in treating cervical radiculopathy in randomized controlled trials was investigated. We searched 16 databases up to August 22, 2022. Of the 2,644 studies retrieved, 22 randomized controlled trials (2,483 participants) were selected. Quality assessments were performed using Cochrane's risk-of-bias tool and RevMan 5.4 software. Outcome measures in the included studies typically showed TEA had a significant therapeutic effect compared with simple acupuncture and other remedies, and TEA was better than sham TEA. Catgut and polydioxanone had no difference in effectiveness, however, catgut was considered to be less safe. TEA was shown to be more therapeutic when inserted deeper into the skin. Ultrasound guided TEA was more effective and safer than conventional TEA, and using a flat blade needle was better than conventional needles for TEA. No serious adverse events were reported from using TEA, and only a few mild side effects were observed. However, the limited number and heterogeneity of the included studies, together with the unclear methodological quality, indicate that higher-quality studies need to be conducted to determine the effectiveness and safety of TEA for cervical radiculopathy.

• Biomolecules & Therapeutics
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• 제30권5호
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• pp.418-426
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• 2022

Chimeric antigen receptor T (CAR-T) cell therapy is one of the promising anticancer treatments. It shows a high overall response rate with complete response to blood cancer. However, there is a limitation to solid tumor treatment. Additionally, this currently approved therapy exhibits side effects such as cytokine release syndrome and neurotoxicity. Alternatively, bispecific antibody is an innovative therapeutic tool that simultaneously engages specific immune cells to disease-related target cells. Since programmed death ligand 1 (PD-L1) is an immune checkpoint molecule highly expressed in some cancer cells, in the current study, we generated αCD3xαPD-L1 bispecific antibody (BiTE) which can engage T cells to PD-L1⁺ cancer cells. We observed that the BiTE-bound OT-1 T cells effectively killed cancer cells in vitro and in vivo. They substantially increased the recruitment of effector memory CD8⁺ T cells having CD8⁺CD44⁺CD62L^low phenotype in tumor. Interestingly, we also observed that BiTE-bound polyclonal T cells showed highly efficacious tumor killing activity in vivo in comparison with the direct intravenous treatment of bispecific antibody, suggesting that PD-L1-directed migration and engagement of activated T cells might increase cancer cell killing. Additionally, BiTE-bound CAR-T cells which targets human Her-2/neu exhibited enhanced killing effect on Her-2-expressing cancer cells in vivo, suggesting that this could be a novel therapeutic regimen. Collectively, our results suggested that engaging activated T cells with cancer cells using αCD3xαPD-L1 BiTE could be an innovative next generation anticancer therapy which exerts simultaneous inhibitory functions on PD-L1 as well as increasing the infiltration of activated T cells having effector memory phenotype in tumor site.

• 예술인문사회 융합 멀티미디어 논문지
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• 제7권2호
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• pp.515-523
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• 2017

문학을 활용하는 실습현장에서는 문학의 전문이 아니라 일부분을 발췌해서 사용한다. 따라서 어느 부분을 기억힌트로 활용할 것인지, 어떻게 제시하는 것이 효과적인지에 관한 실증적인 지침이 필요하다. 본 연구를 위해서 헤세의 삶 전반과 그의 인물들을 문학치료적인 관점으로 들여다보았다. 첫째, 헤세의 작품 속 인물을 분석하는 과정에서 자가치유적인 삶을 살아가고 있는 헤세의 모습을 만날 수 있었다. 또한 그는 쓰기, 그리기, 연주하기, 감상하기, 명상하기, 걷기 등과 함께하는 다매체적인 삶을 살았다. 둘째, 연구자는 헤세와 그의 작품을 활용한 문학치료 콘텐츠를 조현병 환자들에게 적용해 보았다. 임상에서 활용한 매체는 헤세의 작품 속에서 추출한 것이었다. 참여자들은 헤세의 작품에 대해 정서적 공감을 할 수 있었으며 나아가 참여자들간의 상호공감나누기도 가능하였다. 헤세가 보여주는 다매체적인 특성과 활용방법은 현대인의 정신건강을 증진하고 병리적인 문제를 치유하기 위해서 중요한 인문학적 자원이 될 것이다.

• Genomics & Informatics
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• 제21권1호
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• pp.9.1-9.13
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• 2023

Matrix metalloproteinase-9 (MMP-9) is a zinc and calcium-dependent proteolytic enzyme involved in extracellular matrix degradation. Overexpression of MMP-9 has been confirmed in several disorders, including cancers, Alzheimer's disease, autoimmune diseases, cardiovascular diseases, and dental caries. Therefore, MMP-9 inhibition is recommended as a therapeutic strategy for combating various diseases. Cinnamic acid derivatives have shown therapeutic effects in different cancers, Alzheimer's disease, cardiovascular diseases, and dental caries. A computational drug discovery approach was performed to evaluate the binding affinity of selected cinnamic acid derivatives to the MMP-9 active site. The stability of docked poses for top-ranked compounds was also examined. Twelve herbal cinnamic acid derivatives were tested for possible MMP-9 inhibition using the AutoDock 4.0 tool. The stability of the docked poses for the most potent MMP-9 inhibitors was assessed by molecular dynamics (MD) in 10 nanosecond simulations. Interactions between the best MMP-9 inhibitors in this study and residues incorporated in the MMP-9 active site were studied before and after MD simulations. Cynarin, chlorogenic acid, and rosmarinic acid revealed a considerable binding affinity to the MMP-9 catalytic domain (ΔG_binding < -10 kcal/ mol). The inhibition constant value for cynarin and chlorogenic acid were calculated at the picomolar scale and assigned as the most potent MMP-9 inhibitor from the cinnamic acid derivatives. The root-mean-square deviations for cynarin and chlorogenic acid were below 2 Å in the 10 ns simulation. Cynarin, chlorogenic acid, and rosmarinic acid might be considered drug candidates for MMP-9 inhibition.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.4025-4030
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• 2012

Background and Objective: Cardiotoxicity and oxidative stress is a life-threatening side effect of doxorubicin (DOX). We investigate the effects of short-term exercise as therapeutic tool for improvement of cardioprotection against DOX-induced cardiotoxicity in the rat. Methods: Wistar males (weighing $257{\pm}28g$) were divided into six groups: (1) control+placebo (2) control+DOX $10mg.kg^{-1}$ (3) control+DOX $20mg.kg^{-1}$ (4) training+placebo (5) training+ DOX$10mg.kg^{-1}$ (6) training+DOX $20mg.kg^{-1}$. Cardiotoxicity was induced by DOX (10 and $20mg.kg^{-1}$). The rats in groups 4, 5 and 6 experienced treadmill running of 25 to $39min.day^{-1}$ and 15 to $17m.min^{-1}$, 5 days/wk for 3 wk. At the end of the endurance training program, rats in the 1 and 4 groups, in the 2 and 5 groups and in the 3 and 6 groups received saline solution, DOX $10mg.kg^{-1}$ and DOX $20mg.kg^{-1}$, respectively. Result: DOX administration (10 and $20mg.kg^{-1}$) caused significant increase in MDA and Apelin, an insignificant increase in NO and a significant decrease in SOD, as compared to the C+P group. Three weeks of the pretreatment endurance exercise resulted in a significant increase of Apelin and SOD, an insignificant increase of NO and an insignificant decrease of MDA, as compared to the C+P group. Furthermore, after three weeks of endurance training and DOX treatment with $10mg.kg^{-1}$ and $20mg.kg^{-1}$, a significant increase in apelin and SOD, and a significant decrease in MDA were detected in comparison to C+DOX10 and/or C+DOX20 groups. There was a significant difference between DOX$10mg.kg^{-1}$ and DOX$20mg.kg^{-1}$ treatments in MDA levels only. Conclusion: Pretreatment exercise may improve myocardial tolerance to DOX-induced cardiotoxicity by inhibition of oxidative stress and up-regulation of antioxidants in heart tissue.

• Radiation Oncology Journal
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• 제19권1호
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• pp.34-39
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• 2001

목적 : 간암을 대상으로 한 고식적인 방사선치료가 아닌 정위방사선치료에 대한 보고는 매우 드물다. 이에 저자들은 원발성 간암환자들을 대상으로 정위방사선치료에 대한 예비결과를 알아보고자 하였다. 대상 및 방법 : 1999년 7월부터 2000년 3월까지 성모병원 치료방사선과에서 두개외 종양으로 정위방사선치료를 33명의 환자들에서 시행하였으며 그중 13명의 원발성 간암환자들을 대상으로 하였다. 대상환자들의 성별은 남자가 12명, 여자가 1명이었으며 연령분포은 $44\~66$세(중앙값:59세)였고 종양의 크기는 $10\~825\;cc$ (평균 : 185 cc)였다. 정위방사선치료는 1회 $3\~5\;Gy$를 $90\%$의 등선량 곡선에 기준하여 주 3-5회씩, $2\~3$주 동안 조사선량의 범위는 $30\~50\;Gy$이고 중앙선량은 50 Gy였다. 결과 : 추적관찰기간은 $3\~13$개월이었다(중간추적관찰기간: 8개월). 정위방사선치료 후 전체 환자에서 완전관해 $7.7\%$, 부분관해 $v$, 미세관해 $30.8\%$, 무반응 $7.7\%$였으며 국소제어율(완전관해+부분관해)은 $61.5\%$였다. 혈청 AFP의 치료 전과 치료 후 수치의 변화를 비교한 결과, 전체환자 중 $92.3\%$에서 AFP이 감소하였다. 치료에 따른 부작용은 소화장애가 $84.6\%$, 경한 오심이 $69.2\%$, 일시적인 간 기능의 저하가 $15.4\%$, 미열이 $7.7\%$에서 나타났으나 치명적인 부작용은 없었다. 결론 : 원발성 간암에 대한 정위방사선치료를 시행한 결과 비교적 안전하고 효과적인 치료방법이었다. 그러나 새로운 치료방법으로서 검증하기 위해서는 보다 많은 환자와 장기적인 추적관찰이 필요하리라고 사료된다.

• Tuberculosis and Respiratory Diseases
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• 제44권1호
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• pp.114-123
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• 1997

연구배경 : 기관지내시경 검사는 중환자실의 폐렴이 동반된 환자에서 원인균 동정을 위한 매우 유용한 검사법이다. 내시경적 약식의 기관폐포세척한 검체의 결과를 맹검적인 기도세척의 결과와 비교분석함으로써 폐렴을 가진 중환자실 환자에서의 기관지경 검사의 임상적 의의를 평가해 보고자 하였다. 방법 : 1993년 9월부터 1996년 8윌까지 이화의대부속 목동병원 중환자실에 입원치료를 받았던 환자중 임상적 소견상 폐렴으로 진단적 또는 치료적 목적하에 기관지경 검사를 받았던 28명의 환자를 대상으로 내시경적 기관폐포세척을 포함한 임상적 결과에 대해 후향적으로 조사하였다. 결과 : l. 중환자실에 입원한 주 이유는 호흡부전 및 호흡부전 절박상태가 가장 많았다. 폐렴은 그 유형상 원내폐렴이 16예로 가장 많았고 지역획득폐렴 및 면역타협폐렴이 각 6예씩 있었다. 기관지경 목적상 진단적 목적이 20예 71%를 차지하였고 치료적 목적의 분비물제거가 8예 있었다. 내시경 검사에 따른 합병증의 발생은 미미하였다. 2 맹검적 검체와 내시경적 검체간 균주배양 일치율은 39.3 %에 불과하였고 내시경적 검사로 국균증 및 결핵이 각 2예씩 진단되었다. 3 내시경적 검체결과를 기준할때 다제약제내성 폐렴은 기타균주에 의한 폐렴과 비교시 기계호흡의 적용이 유의하게 빈번히 말생하였다. 4 원내폐렴군은 타군에 비해 기계호흡 적용이 유의하게 많았다. 결론 : 이상의 결과로 폐렴이 동반된 중환자실 환자에서 기관지경을 통한 선택적인 기관폐포세척물 검사 및 치료적 기관분비물의 제거는 진단 및 치료상의 매우 유용한 방편이 될 수 있으며 동정된 균주가 다제약제내성균이거나 원내폐렴형인 경우 기계호흡의 사용과 밀접한 관련이 있을 것으로 생각된다.

• 대한간호학회지
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• 제27권1호
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• pp.178-188
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• 1997

As a descriptive survey, this study was attempted to get basic data necessary to recognize the factors that disturb the therapeutic atmosphere of hospital wards as perceived by nurses and hospitalized patients, to identify differences between the perceptions of the nurses and of patients. The subjects, 159 patients in Pusan National Hospital and 68 nurses working there were sampled between March 18 and April 13, 1996. The tool used to measure the disturbing factors was an amended form of the one developed by Kim, Mae Ja(1983). The differences between each subject's score for each factor were analyzed using means & SD. and the highest 3 items above the mean score for each factor were collected and compared. The results are described below : 1. Subject's perception of main disturbing factors : patients reported that the main factors were 'loss of role & economic trouble', 'the prognosis of disease', 'the change of daily life' but nurses replied that the main factors were' the prognosis of disease', 'the communication trouble with the medical team & interpersonal relationships'. 'The change of daily life' was not a perceved factor by nurses, but ranked third by the patients. 2. Subject's perception degree of each disturbing factor : (1) among the items related to interpersonal relationship. the patient group reported that the worst disturbance was dur to severely ill patients in the same room' but the nurse group regarded 'greed to monopolize wheelchairs or other supplies' as the worst disturbance. (2) among the items related to physical factors. the patient group regarded 'limitations to wash their body, physical pain and limitations in physical activity' as the worst disturbance, but the nurse group regarded' physical pain', and 'limitations to activity or change of appearance' as the worst disturbance. (3) among the items related to the change of daily activity, the patient group regarded 'the boredom of hospitalization or infavorable diet' as the worst disturbance, but the nurse group regarded 'too much noise or unclean room' as the worst disturbance. (4) among the items related to the communication trouble with medical team, the patient group regarded 'the ignorance of their disease due to poor information. the inability to understand the language of the medical team or the difficulty in seeing physician in time' as the worst disturbance, but the nurse group regarded 'the inability to trust physicians and physician's poor attention to patients' as the worst disturbance.