• 한국임상수의학회지
• /
• 제24권3호
• /
• pp.384-391
• /
• 2007

This study was conducted to clarify the effects of the Korean raisin (Hovenia dulcis Thunb) extracts and polyherbal extracts consisted of 6 types herbal extracts including HDT (HDTmix) on $CCl_4$ induced organ damages. Extracts were prepared by autoclave ($121^{\circ}C$, 15 psi, 3 hours) and filtered with nylon cloth and filter paper then freezing dried. Male Sprague-Dawley rats $(200^{\circ}{\ae}20g)$ were used as experimental groups, which were divided into 5 groups; intact control group (100 mg/kg), $CCl_4$ dosing group (100 mg/kg), $CCl_4$ dosing after HDT extract dosing group (100 mg/kg), $CCl_4$ dosing after HDTmix dosing group (100 mg/kg), Silymarin dosing group (5 mg/kg) after all test articles were orally dosed once a day for 28 days. They were sacrifiled under ether anesthesia. HDT extracts and HDTmix dramatically inhibits the $CCl_4$ intoxicated hepato/nephropathies with immuno-suppress changes on the spleen. They showed more dramatical protective effects on most of specific organs compared to that of Silymarin 5 mg/kg except for hepatoprotective effects in which, quite similar effects were detected. In addition, HDT extracts showed synergic effects with other types of herbal extracts because HDTmix showed more favorable protective effects on the all specific organs showing $CCl_4-related$ histopathological changes compared to HDT extracts.

• 대한한방내과학회지
• /
• 제41권3호
• /
• pp.339-349
• /
• 2020

Objective: This study aimed to evaluate anti-inflammatory and antitussive expectoration effects of Friltillariae Thunbergii Bulbus (FTB) in a mouse model. Materials and Methods: To evaluate the anti-inflammatory effects of the FTB, we conducted in vitro experiments using RAW264.7 cells. An MTT assay and enzyme-linked immunosorbent assay (ELISA) were carried out to examine the anti-inflammatory effects of FTB. The expectorant effect on phenol red secretion, the antitussive effect on cough induced by ammonia solution, and leukocyte increased inhibition effects in acute airway inflammation in the animal model were confirmed. Results: FTB did not show cytotoxicity in the experimental group at 10, 30, 50, 100, 300, or 500 ㎍/ml and significantly inhibited the increase of NO, TNF-α and IL-6 in the experimental groups at 30, 50, 100, 300, and 500 ㎍/ml concentrations. In sputum, cough, and acute airway inflammation animal models, FTB significantly increased phenol red secretion in the 400 mg/kg administration group. FTB significantly reduced the number of coughs and significantly increased cough delay time in both 200 and 400 mg/kg dose groups. FTB decreased the white blood cell count in BALF (bronchoalveolar lavage fluid) in the 400 mg/kg administration group. Conclusion: Our study revealed that FTB elicits antitussive and expectorant effects by inhibiting inflammatory cytokines, increasing sputum secretion, suppressing cough, and reducing inflammatory cells. We concluded that FTB is a highly promising agent for respiratory tract infection with therapeutic opportunities.

• 한국산학기술학회논문지
• /
• 제17권10호
• /
• pp.562-568
• /
• 2016

본 연구의 목적은 가상현실과 로봇보조재활치료를 이용하여 뇌졸중 후 푸셔 증후군에 대한 치료적인 효과를 조사하고자 하기 위함이다. 총 10명의 뇌졸중 후 푸셔 증후군을 보이는 환자가 모집되었다. 환자는 각각 5 명씩 로봇보조재활치료와 대조군으로 배정되었다. 실험군은 로봇보조재활치료와 일반적인 재활치료를 함께 받았으며, 대조군은 하루 2회 일반적인 재활치료를 받았다. 중재 시간은 30분간 진행되었으며, 주 5회, 4주간 시행하였다. 치료 전후 변화는 Scale for contraversive pushing (SCP), Berg balance scale (BBS), falling index (FI), Timed up and go test (TUG)을 이용하여 측정하였다. 4 주간의 중재 치료 후 로봇보조재활치료 군에서 SCP (p=0.046), BBS (p=0.046), FI (p=0.038), TUG (p=0.038)은 대조군에 비하여 유의하게 향상되었다. 또한 SCP와 BBS (p=0.024), FI (p=0.039), TUG (p=0.030)는 유의한 상관관계가 관찰되었다. 결과적으로 가상현실을 이용한 로봇보조재활치료는 일반적인 재활치료에 비하여 뇌졸중 후 푸셔 증후군을 회복하는데 더 도움이 되었으며, 푸셔 증후군의 회복은 균형과 보행기능의 향상과 관련이 있었다.

• 대한응급의학회지
• /
• 제28권5호
• /
• pp.457-466
• /
• 2017

Purpose: Traumatic brain injury (TBI) is a significant cause of morbidity and mortality worldwide. Severity of the initial insult is one of the most significant factors affecting outcome following TBI. In order to investigate the mechanisms of cellular injury and develop novel therapeutic strategies for TBI, we designed a standardized animal TBI model and evaluated histological and functional outcomes according to the degree of impact severity. Methods: Male adult C57Bl/6 mice underwent controlled cortical impact (CCI) at varying depths of deflection (1.0-2.0 mm). We performed hematoxylin and eosin staining at 7 days after recovery from TBI. Neurobehavioral characterization after TBI was analyzed by the Barnes maze test, passive avoidance test, open field test, rotarod test, tail suspension test, and light/dark test. Results: We observed a graded injury response according to the degree of deflection depths tested (diameter, 3 mm; velocity, 3 m/s; and duration, 500 ms) compared to sham controls. In the Barnes maze test, the severe TBI (2 mm depth) group showed reduced spatial memory as compared with the sham and mild TBI (1 mm depth) groups at 7 days after TBI. There was a significant difference in the results of the open field test and light/dark test among the three groups. Conclusion: Our findings demonstrate that the graded injury responses following TBI resulted in differential histopathological and behavioral outcomes in a mouse experimental CCI model. Thus, a model of CCI with histologic/behavioral outcome analysis may offer a reliable and convenient design for preclinical TBI research involving mice.

• Journal of Ginseng Research
• /
• 제39권1호
• /
• pp.46-53
• /
• 2015

Background: Glucocorticoids (GCs) are commonly used in many chemotherapeutic protocols and play an important role in the normal regulation of bone remodeling. However, the prolonged use of GCs results in osteoporosis, which is partially due to apoptosis of osteoblasts and osteocytes. In this study, effects of Korean Red Ginseng (KRG) on GC-treated murine osteoblastic MC3T3-E1 cells and a GC-induced osteoporosis mouse model were investigated. Methods: MC3T3-E1 cells were exposed to dexamethasone (Dex) with or without KRG and cell viability was measured by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Realtime polymerase chain reaction was performed to evaluate the apoptotic gene expression; osteogenic gene expression and alkaline phosphatase (ALP) activity were also measured. Western blotting was performed to evaluate the mitogen-activated protein kinase (MAPK) proteins. A GC-induced osteoporosis animal model was used for in vivo study. Results and conclusion: The MTT assay revealed that Korean Red Ginseng (KRG) prevents loss of cell viability caused by Dex-induced apoptosis in MC3T3E1 cells. Real-time polymerase chain reaction data showed that groups treated with both Dex and KRG exhibited lower mRNA levels of caspase-3 and -9, whereas the mRNA levels of Bcl2, IAPs, and XIAP increased. Moreover, groups treated with both Dex and KRG demonstrated increased mRNA levels of ALP, RUNX2, and bone morphogenic proteins as well as increased ALP activity in MC3T3-E1 cells, compared to cells treated with Dex only. In addition, KRG increased protein kinase B (AKT) phosphorylation and decreased c-Jun N-terminal kinase (JNK) phosphorylation. Moreover, microcomputed tomography analysis of the femurs showed that GC implantation caused trabecular bone loss. However, a significant reduction of bone loss was observed in the KRG-treated group. These results suggest that the molecular mechanism of KRG in the GC-induced apoptosis may lead to the development of therapeutic strategies to prevent and/or delay osteoporosis.

• Journal of Chest Surgery
• /
• 제52권4호
• /
• pp.221-226
• /
• 2019

Background: Accurate mediastinal lymph node staging is vital for the optimal therapy and prognostication of patients with lung cancer. This study aimed to determine the preoperative risk factors for pN2 disease, as well as its incidence and long-term outcomes, in patients with clinical N0-1 non-small cell lung cancer. Methods: We retrospectively analyzed patients who were treated surgically for primary non-small cell lung cancer from November 2005 to December 2014. Patients staged as clinical N0-1 via chest computed tomography (CT) and positron emission tomography (PET)-CT were divided into two groups (pN0-1 and pN2) and compared. Results: In a univariate analysis, the significant preoperative risk factors for pN2 included a large tumor size (p=0.083), high maximum standard uptake value on PET (p<0.001), and central location of the tumor (p<0.001). In a multivariate analysis, central location of the tumor (p<0.001) remained a significant preoperative risk factor for pN2 status. The 5-year overall survival rates were 75% and 22.9% in the pN0-1 and pN2 groups, respectively, and 50% and 78.2% in the patients with centrally located and peripherally located tumors, respectively. In a Cox proportional hazard model, central location of the tumor increased the risk of death by 3.4-fold (p<0.001). Conclusion: More invasive procedures should be considered when preoperative risk factors are identified in order to improve the efficacy of diagnostic and therapeutic plans and, consequently, the patient's prognosis.

• 재활치료과학
• /
• 제8권1호
• /
• pp.51-62
• /
• 2019

목적: 본 연구의 목적은 다양한 인지기능 수준을 가진 고령자를 위한 실감객체 기반 인지재활시스템을 개발하고, 사용성을 검증하는 것이다. 방법: 사용성 연구는 시스템의 강점과 약점을 확인하기 위해 15명의 환자 집단과 4명의 작업치료사 집단에 설문조사를 실시하였으며 응답에 근거한 수정을 통해 시스템을 보완 및 개선하였다. 응답자들은 세 종류의 훈련-실행기능 훈련, 기억력 훈련, 집중력 훈련을 20분~30분간 경험해본 후, 구조화된 설문에 응답하였으며 설문의 영역은 각 훈련 영역별 콘텐츠, 중앙처리장치이자 화면 제시 매체인 태블릿 PC 및 실감객체를 포함하는 하드웨어, 시스템 이용에 따른 만족도로 구성되었다. 결과: 설문 응답 결과 환자 집단과 작업치료사 집단 모두에게 가장 흥미로웠던 영역은 실행기능으로 나타났고 가장 흥미롭지 않은 영역은 집중력으로 나타났다. 하드웨어 사용성 설문에서 태블릿 PC의 화면의 크기가 부적절하다는 응답이 6건, 실감객체의 크기가 부적절하다는 응답이 5건으로 나타났다. 시스템 이용에 대한 만족도 설문에서 전체 응답자의 40%가 시스템에 만족하는 것으로 응답하였다. 결론: 본 시스템은 훈련과제를 수행하기 위해 필요한 실감객체 도구의 조작이 용이하다는 점과 사용자의 요구에 따라 인지훈련의 영역을 쉽게 선택하여 훈련할 수 있다는 점, 사용자의 수행도에 의해 난이도가 자동으로 조정된다는 점을 특징으로 갖는다. 본 시스템에서 입력장치로 사용되는 실감객체도구는 양손에 쥐고 사용함으로써 훈련환경을 자연스러운 일상에서의 도구 사용과 유사하도록 고안하였다. 본 시스템은 지역사회 인지 재활전문가 부족의 대안으로 사용가능 할 것으로 기대된다.

• 재활치료과학
• /
• 제10권1호
• /
• pp.115-127
• /
• 2021

목적 : 본 연구는 원격재활에 기반 한 작업치료가 뇌졸중 환자의 실행기능, 일상생활수행, 작업수행에 영향을 미치는지 알아보고자 한다. 연구방법 : 지역사회에 거주하며, 재활병원에 외래로 통원하는 환자 30명을 실험군과 대조군에 각각 15명씩 무작위 할당하여 연구를 진행하였다. 실험군 집단과 실험군의 중재는 모두 주 3회 18회기로 실시하였다. 실험군 15명에게는 전통적 작업치료와 함께 더불어 가정에서의 원격재활 기반 프로토콜을 적용하였다. 대조군 15명은 전통적 작업치료만 실시하였다. 실험 참가자의 실행기능과 수단적 일상생활수행은 실행기능 수행평가 한국판(Executive Function Performance Test Korean version; EFPT-K)으로 측정하였고, 기본적 일상생활활동은 수정된 바델 지수(Modified Barthel Index; MBI), 작업수행은 클라이언트 중심의 과제수행을 측정하기 위해서 캐나다 작업수행 측정(Canadian Occupational Performance Measure; COPM)을 이용하였다. 결과 : 중재 후 실험군과 대조군은 모두 실행기능, 수단적 일상생활활동, 작업수행에서 유의한 변화가 있었으나(p<.05), 기본적 일상생활수행에서는 유의한 변화가 없었다. 집단 간 비교에서는 작업수행에서만 실험군이 대조군보다 유의하게 더 많은 증진이 나타났다. 결론 : 원격재활과 작업치료는 뇌졸중 환자의 실행기능, 수단적 일상생활수행, 작업수행의 향상에 긍정적인 영향을 미친다. 작업치료에 병행된 원격재활은 뇌졸중 환자의 작업수행에 추가적인 효과가 있음을 근거로써 제시한다.

• Korean Journal of Otorhinolaryngology-Head and Neck Surgery
• /
• 제60권5호
• /
• pp.209-214
• /
• 2017

Background and Objectives Tinnitus retraining therapy (TRT) is one of the most effective treatment modalities of tinnitus based on the neurophysiological model proposed by Jastreboff and Hazell. This study was performed to evaluate the effect of counselor factor on treatment outcomes of TRT. Subjects and Method The total of 78 patients who had TRT from three different counselors in a tinnitus clinic of tertiary referral center from Jan 2015 to Dec 2015 were included in this study. Their medical records were retrospectively reviewed to evaluate the therapeutic response to TRT. Results Among 78 patients who were followed-up for more than 6 months, 47, 20, and 11 patients were treated by counselors A, B, C (all ENT specialists), respectively. Counselor A had 15-year-experience of TRT counseling, whereas counselor B and C were well trained but beginners of TRT counseling. Initial clinical characteristics including Tinnitus Handicap Inventory (THI) and tinnitus Visual Analogue Scale (VAS) scores of the patients among three groups were not significantly different. Treatment responses evaluated via THI and most of the tinnitus VAS scores after at least 6 months after TRT were significantly improved in all three groups (p<0.05) with no significant difference between the senior (A) and junior (B, C) group. Conclusion TRT seems to be an effective treatment modality of tinnitus even in this short term follow-up study. Treatment outcomes of TRT may not depend on the counselors once they are well trained and follow the same protocol.

• 인간행동과 음악연구
• /
• 제19권1호
• /
• pp.93-112
• /
• 2022

본 연구의 목적은 화음의 전개 수준에 따라 감상자가 지각하는 정서 차이를 비교하는 데 있다. 만 19세-29세 사이 음악 전공자 73명, 음악 비전공자 71명의 성인 144명을 대상으로, 조성(장조-단조)과 화음 전개 수준(낮음-높음)이 서로 다른 총 네 가지의 음악을 온라인으로 제공하여 실험을 진행하였다. 연구 참여자들은 각 음악에 대해 정서적 역동감, 각성, 화음이 감정에 영향을 미치는 수준, 선호도의 총 네 가지 항목을 리커트 7점 척도로 평가하였다. 연구 결과, 첫째, 화음 전개 수준과 조성에 따라 감상자가 지각하는 정서적 역동감, 각성, 화음이 감정에 영향을 미치는 수준, 선호도에서 유의한 차이가 있었다. 둘째, 전공 배경을 기준으로 하여 음악 전공자와 음악 비전공자로 그룹을 나누고 화음의 전개 수준 차이에 따른 그룹 간 정서 지각을 분석하였을 때, 정서적 역동감에서 그룹 간 유의한 차이가 나타났으나, 각성, 감정에 영향을 미치는 수준, 선호도에서의 그룹 간 상호작용 효과는 유의하지 않은 것으로 나타났다. 결론적으로 본 연구는 조성, 감상자의 외생 변인을 통제하여 제공한 음악에서 화음의 전개 수준에 따른 감상자의 다양한 정서 반응의 차이를 확인할 수 있었다. 본 연구에서 도출된 결과는 내담자의 필요에 따른 음악 선곡 시, 화음의 전개 수준을 다르게 제공하는 기초자료로 활용될 수 있을 것으로 기대된다.