The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Experimental Study on Anti-inflammatory, Antitussive, and Expectoration Effects of Friltillariae Thunbergii Bulbus

절패모(浙貝母)의 항염 및 진해거담 효과에 대한 실험연구

  • Kim, Jin Hoo (Division of Respiratory Medicine, Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University) ;
  • Yang, Won Kyung (Division of Respiratory Medicine, Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University) ;
  • Lee, Su Won (Division of Respiratory Medicine, Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University) ;
  • Lyu, Yee Ran (Division of Respiratory Medicine, Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University) ;
  • Kim, Seung Hyung (Institute of Traditional Medicine and Bioscience, Dae-Jeon University) ;
  • Park, Yang Chun (Division of Respiratory Medicine, Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University)
  • 김진후 (대전대학교 한의과대학 폐계내과학교실) ;
  • 양원경 (대전대학교 한의과대학 폐계내과학교실) ;
  • 이수원 (대전대학교 한의과대학 폐계내과학교실) ;
  • 유이란 (대전대학교 한의과대학 폐계내과학교실) ;
  • 김승형 (대전대학교 동서생명과학연구원) ;
  • 박양춘 (대전대학교 한의과대학 폐계내과학교실)
  • Received : 2020.03.28
  • Accepted : 2020.07.01
  • Published : 2020.06.30
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Abstract

Objective: This study aimed to evaluate anti-inflammatory and antitussive expectoration effects of Friltillariae Thunbergii Bulbus (FTB) in a mouse model. Materials and Methods: To evaluate the anti-inflammatory effects of the FTB, we conducted in vitro experiments using RAW264.7 cells. An MTT assay and enzyme-linked immunosorbent assay (ELISA) were carried out to examine the anti-inflammatory effects of FTB. The expectorant effect on phenol red secretion, the antitussive effect on cough induced by ammonia solution, and leukocyte increased inhibition effects in acute airway inflammation in the animal model were confirmed. Results: FTB did not show cytotoxicity in the experimental group at 10, 30, 50, 100, 300, or 500 ㎍/ml and significantly inhibited the increase of NO, TNF-α and IL-6 in the experimental groups at 30, 50, 100, 300, and 500 ㎍/ml concentrations. In sputum, cough, and acute airway inflammation animal models, FTB significantly increased phenol red secretion in the 400 mg/kg administration group. FTB significantly reduced the number of coughs and significantly increased cough delay time in both 200 and 400 mg/kg dose groups. FTB decreased the white blood cell count in BALF (bronchoalveolar lavage fluid) in the 400 mg/kg administration group. Conclusion: Our study revealed that FTB elicits antitussive and expectorant effects by inhibiting inflammatory cytokines, increasing sputum secretion, suppressing cough, and reducing inflammatory cells. We concluded that FTB is a highly promising agent for respiratory tract infection with therapeutic opportunities.

Keywords

References

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