• Title/Summary/Keyword: theoretical yield

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Determination of Optimum Conditions for Xylose Fermentation by Pichia stipitis (Pichia stipitis에 의한 Xylose 발효의 최적조건 결정)

  • 권순효;유연우서진호
    • KSBB Journal
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    • v.8 no.5
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    • pp.452-456
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    • 1993
  • This study was carried out to optimize the fermentation conditions for direct alcohol fermentation of xylose by Pichia stipitis CBS 5776. The best cell growth and the ethanol production were obtained under 0.05 VVM aeration and 300rpm agitation at $30^{\circ}C$ using 100 g/l xylose medium of the initial pH 5.0. In the above condition, the maximum specific growth rate and maximum cell concentration were 0.14hr-1 and $1.3 \times109$ cells/ml, respectively. Pichia stipitis CBS 5776 also produced 40.2g/l ethanol utilizing about 96% of 100g/l xylose after 72hr fermentation. At this point, the overall volumetric ethanol productivity was 0.56g/1-hr and the ethanol yield was 0.42 g-ethanol/g-xylose consumed, which corresponds to 82% of the theoretical yield.

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Application of Oxygen Uptake Rate Measured by a Dynamic Method for Analysis of Related Fermentation Parameters in Cyclosporin A Fermentation:Suspended and Immobilized Cell Cultures

  • Chun, Gie-Taek;Agathos, S.N.
    • Journal of Microbiology and Biotechnology
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    • v.11 no.6
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    • pp.1055-1060
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    • 2001
  • Experimental data for the on-line estimation of cell concentration and growth rate are presented. For this purpose, we utilized the on-line calculation of the oxygen uptake rate (OUR), which was derived from a liquid phase dynamic mass balance for the oxygen during the active growth phase in cyclosporin A (CyA) fermentation. The cell yield coefficient, based on the oxygen $(Y_{x/o})$for both suspended and immobilized cells of Tolypocladium inflatum, was estimated as $1.9 gDCW/gO_2$ from a very good linear correlation between the cell mass produced and the total oxygen consumed. The calculated yield showed a good agreement with the value of $(Y_{x/o})$ generated from the correlation between the cell growth rate and the oxygen uptake rate. In addition, further experimental data are given, which were also applied to determine the specific oxygen uptake rate of T. inflatum cells during the exponential phase of CyA fermentation. A theoretical basis for the analysis of these fermentation parameters is also provided.

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Central angle effect on connection behavior of steel box beam-to-circular column

  • Hwang, Won-Sup;Kim, Young-Pil;Yoon, Tae-Yang
    • Structural Engineering and Mechanics
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    • v.32 no.4
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    • pp.531-547
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    • 2009
  • This paper presents the experimental results on the strength behavior and failure modes of box beam-to-circular column connections in steel piers. Previous research introduced parameters such as joint central angles, extension of horizontal stiffeners, and use of equivalent web depth, which ignored strength behavior and failure modes of box beam-to-circular column connections. The use of equivalent web depth $d_2$ is not reasonable when central angle ${\alpha}$ is closer to $90^{\circ}$; therefore, a monotonic loading test has been performed for eight connection specimens. From the test, it is identified that the connection with the circular column is stronger than the connection with the box-sectioned substitution column. Also, the strength of the beam-to-column connections with horizontal stiffeners is higher than the one of the no column stiffeners. The concrete-filled effect of box beam-to-circular column connection is also investigated, and the experimental yield strength of the connection is compared with the theoretical one. Also, more a reasonable equivalent web depth is suggested. The failure modes of connection are clearly defined.

Production of Ethanol from D-Xylose by Fusarium sp. (Fusarium sp.에 의한 D-Xylose로부터 Ethanol 생산)

  • 이상협;이왕식;방원기
    • Microbiology and Biotechnology Letters
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    • v.15 no.5
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    • pp.340-345
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    • 1987
  • Microorganisms capable of utilizing D-xylose as a sole carbon and energy source were isolated to ferment D-xylose directly to ethanol. Among them, the strain, which showed the best ability to pro-duce ethanol, was selected and was identified as Fusarium sp. The optimal conditions for the pro-duction of ethanol were 8.0 of initial pH, 33$^{\circ}C$ of temperature, and 2% of substrate concentration. Under this optimal condition, the following results were obtained : maximum ethanol concentration, 7.0g/$\ell$; ethanol yield, 0.35g of ethanol per g of D-xylose (68.6% of theoretical); biomass yield, 0.27g of dry biomass per g of D-xylose.

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Ethanol Production from Seaweed, Enteromorpha intestinalis, by Separate Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) with Saccharomyces cerevisiae

  • Cho, YuKyeong;Kim, Min-Ji;Kim, Sung-Koo
    • KSBB Journal
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    • v.28 no.6
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    • pp.366-371
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    • 2013
  • Ethanol productions were performed by separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes using seaweed, Enteromorpha intestinalis (sea lettuce). Pretreatment conditions were optimized by the performing thermal acid hydrolysis and enzymatic hydrolysis for the increase of ethanol yield. The pretreatment by thermal acid hydrolysis was carried out with different sulfuric acid concentrations in the range of 25 mM to 75 mM $H_2SO_4$, pretreatment time from 30 to 90 minutes and solid contents of seaweed powder in the range of 10~16% (w/v). Optimal pretreatment conditions were determined as 75 mM $H_2SO_4$ and 13% (w/v) slurry at $121^{\circ}C$ for 60 min. For the further saccharification, enzymatic hydrolysis was performed by the addition of commercial enzymes, Celluclast 1.5 L and Viscozyme L, after the neutralization. A maximum reducing sugar concentration of 40.4 g/L was obtained with 73% of theoretical yield from total carbohydrate. The ethanol concentration of 8.6 g/L of SHF process and 7.6 g/L of SSF process were obtained by the yeast, Saccharomyces cerevisiae KCTC 1126, with the inoculation cell density of 0.2 g dcw/L.

Dynamic elastic-plsstic Crack Curving Phenomenon (탄소성 동적 균열전파의 만곡현상)

  • 이억섭;정형진
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1996.04a
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    • pp.704-708
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    • 1996
  • The elastic dynamic crack curving could be obtained by controlling the loading rate, the initial crack-tip blunting to store much energy before crack initiation and the magnitude of reflected wave from finite boundaries. However there is no theoretical and experimental elastic-plastic dynamic curving study. This paper proposes a specimen geometryfor a study of dynamic elastic-plastic crack curving and presents a preliminary result. The specimen has a blunt physical crack tip on a side, and a round notch tip on the other side. From the experiment using this specimen, it is found that the narrow plastic zone ahead of the round notch tip produces the change of load direction and anti-symmetricity of the dynamic isochromatics, and each result causes the crack curving phenomenon. After a certain time, as the elastic-plastic crack gets close to the round notch tip near, the degree of the crack curving get larger. The elastic reack curving propagates more sensitively to the surround of crack tip than the plastic crack curving does. The cynamic elastic-plastic crack curving is found to be proportional to the CTOA(the crack tip opening angle). The dynamic elastic-plastic crack may propagate in the direction perpendicular to the loading. An apparant strip yield zone which is similar to the Dugdale strip yield zone is noted ahead of the physical crack tip.

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Theoretical Study of Solvent Effect on Yield of Oxidative Addition Reaction (산화첨가반응의 수득률에 미치는 용매효과에 관한 이론적 연구)

  • Lee, Chul-Jae;Kim, Byung-So
    • Applied Chemistry for Engineering
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    • v.21 no.5
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    • pp.586-589
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    • 2010
  • In present work, oxidative addition reaction of 1,3-cycleohexandion (1,3-CHD) with ethyl vinyl ether (EVE) was attempted utilizing $Ag_2CO_3$/celite (SC) reagent. In order to optimize reaction conditions, we surveyed several solvents for the production of dihydrofuran with the (SC) system. The yield of the acetonitrile (AN), dimethyl sulfoxide (DMSO), benzene (BZ), and heptane (HT) are given 78, 40, 15, and 10%, respectively. Therefore, we studied the solvent effects on yields by using PM3 and ZINDO/1 parameter of semi-empirical method of HyperChem7.0 molecular modeling program.

The Structural Behavior of CFCT Column to H-Beam Connections With Longitudinal Rib of Column at Joint (종리브로 보강한 콘크리트충전원형강관기둥-H형강보 접합부의 구조적 거동에 관한 연구)

  • Kang, Hyun Sik;Moon, Tae Sup
    • Journal of Korean Society of Steel Construction
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    • v.10 no.2 s.35
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    • pp.291-301
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    • 1998
  • This paper is a study on the structural behavior of CFCT(Concrete-Filled Circular Tubular) column to H-beam connections with longitudinal rib. The important parameters are being longitudinal rib or not. variable column thickness(5.8mm. 9.2mm. 12.0mm. 15.0mm) around the joint between CFCT and H-beam and the width of flange to diameter. Test results are summarized for the strength, initial stiffness, failure mode and energy absorption capacities of each specimen. These are compared with the theoretical results(Yield line theory, numerical analysis). Therefore, the purpose of this paper is to investigate the stiffness and the strength of connections to evaluate the structural behavior of the CFCT column to H-beam connections with longitudinal rib.

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Normalised rotation capacity for deformability evaluation of high-performance concrete beams

  • Zhou, K.J.H.;Ho, J.C.M.;Su, R.K.L.
    • Earthquakes and Structures
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    • v.1 no.3
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    • pp.269-287
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    • 2010
  • High-strength concrete (HSC) is becoming more popular in the construction of beams and columns of tall buildings because of its higher stiffness and strength-to-weight ratio. However, as HSC is more brittle than normal-strength concrete (NSC), it may adversely affect the flexural ductility and deformability of concrete members. Extended from a series of theoretical study conducted on flexural ductility of concrete beams, the authors would in this paper investigate the effects of some critical factors including the degree of reinforcement, confining pressure, concrete and steel yield strength on the flexural deformability of NSC and HSC beams. The deformability, expressed herein in terms of normalised rotation capacity defined as the product of ultimate curvature and effective depth, is investigated by a parametric study using nonlinear moment-curvature analysis. From the results, it is evident that the deformability of concrete beams increases as the degree of reinforcement decreases and/or confining pressure increases. However, the effects of concrete and steel yield strength are more complicated and dependent on other factors. Quantitative analysis of all these effects on deformability of beams has been carried out and formulas for direct deformability evaluation are developed. Lastly, the proposed formulas are compared with available test results to verify its applicability.

Flexural bearing capacity of diaphragm-through joints of concrete-filled square steel tubular columns

  • Rong, Bin;Liu, Rui;Zhang, Ruoyu;Chen, Zhihua;Apostolos, Fafitis
    • Steel and Composite Structures
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    • v.20 no.3
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    • pp.487-500
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    • 2016
  • In order to investigate the flexural bearing capacity of panel zone of diaphragm-through joint between concrete filled square steel tubular column and steel beam, four specimens were tested under static tension loads to study the mechanical properties and bearing capacity of diaphragm-through joints with a failure mode of panel zone. Finite element models of these specimens were developed to simulate the test and compare the predicted failure modes, load-displacement curves and bearing capacities with the experimentally observed. It was found that the tensile load from the steel beam flange is mainly shared by the square steel tube and the diaphragm. The diaphragm plastic zone appears along the cross-section lines enclosed by the square steel tube and the influence of steel beam web on the plastic zone of the steel tube is significant and cannot be neglected. Computational models of yield lines on square steel tube and diaphragm are established based on the distribution pattern of the plastic zone, and an analytical method for the evaluation of the bearing capacity of the joint is proposed. The theoretical results and the experimental data are compared and found in good agreement.