• 한국미생물·생명공학회지
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• 제17권3호
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• pp.173-177
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• 1989

HeLa 세포주의 연속 배양시 세포수가 배지의 이동속도가 증가함에 따라 감소하는 현상을 나타냈으며, 최대 세포수를 유지할 때의 dilution rate은 0.012(1/h)로 wash-out인 0.050(1/h)보다 극히 낮으며, dilution rate이 0.030(1/h)일 때 2.0(mL of cells/L/h)의 최대 세포 생산속도를 보였다. 또한 낮은 배지 이동속도에서 세포수의 감소에 따른 maintenance term의 존재를 확인했다. 더불어 packed cell volume파 산소소비속도의 측정값이 실제 세포증식과 밀접한 관계가 있음이 입증되어 간접방법에 의한 생육도치 측정이 가능하게 되었다. 또한 산소 yield model에 의해 최대 산소 수율, $Y_{O2}^{max}$과 maintenance 산소소비속도, m$_{O2}$가 각각 4.1$\times$$10^5$(cells/mmole $O_2$)와 10.71$\times$$10^{-9}$(mmole $O_2$/ cells/h)로 측정되었다.

• Structural Engineering and Mechanics
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• 제85권5호
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• pp.679-691
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• 2023

Many codes allow designers to use the bending moment diagram computed by elastic analysis and modify it by a certain amount of moment redistribution (MR) to account for plastic behaviour of continuous beams. However, several researchers indicated that the MR at the ultimate limit state (𝛽_u) for some beams deviate significantly from the specified values of various codes. This paper examines the applicability of the provisions on 𝛽_u in ACI 318-19 and Eurocode 2 through numerical investigations and comprehensively explores the influencing factors. The results show that some parameters not considered in those codes influence 𝛽_u to a certain extent, where the ratio of tensile reinforcement ratio at intermediate support to tensile reinforcement ratio at midspan (𝜌_s1/𝜌_s2) and load type are crucial parameters to consider. The specific combination of these two parameters may make the codes overestimate or significantly underestimate the 𝛽_u. On the other hand, the yield state of both critical sections is found to have an important influence on the influence degree of each parameter on 𝛽_u. The yield conditions are investigated, and an empirical judgment equation is proposed. In addition, the influence laws of the critical parameters on 𝛽_u have been further proved by theoretical derivation. Finally, due to 𝜀_t is found to have a better linear correlation with 𝛽_u than x_u/d, equations as a function of 𝜀_t for predicting the 𝛽_u of continuous beams under the two loads are proposed, respectively.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2001년도 가을 학술발표회 논문집
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• pp.521-526
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• 2001

The flexural behavior of a strengthened beam that is a reinforced concrete beam with externally bonded carbon fiber sheets, is theoretically and experimentally investigated. A rectangular beam having a width of 20cm depth of 30cm and effective depth of 25cm is chosen. In order to have a variety of beams analyzed, three reinforcement ratios are chosen for the analysis: 1)$\frac{1}{2}$$\rho$$_{max}$, which is the most suitable reinforcement ratio for deflection consideration and the highest reinforcement ratio for practical designing beams as well; 2)$\rho$$_{max}$, which is the lowest reinforcement ratio for design purposes; and 3)the reinforcement ratio halfway from 1) and 2). Carbon fiber sheets with width of 15cm are externally bonded at the bottom fiber of the beam. The effect of the amount of carbon fiber sheets varying from 1 to 4 plies on the flexural capacity of the strengthened beam are also examined. Yield loads, ultimate loads, and flexural rigidities of the strengthened beam from the experimental results are composed with theoretical ones.nes.

• Structural Engineering and Mechanics
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• 제40권4호
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• pp.541-562
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• 2011

In the design of earthquake resistant reinforced concrete (RC) structures, both flexural strength and deformability need to be considered. However, in almost all existing RC design codes, the design of flexural strength and deformability of RC beams are separated and independent on each other. Therefore, the pros and cons of using high-performance materials on the flexural performance of RC beams are not revealed. From the theoretical results obtained in a previous study on flexural deformability of RC beams, it is seen that the critical design factors such as degree of reinforcement, concrete/steel yield strength and confining pressure would simultaneously affect the flexural strength and deformability. To study the effects of these factors, the previous theoretical results are presented in various charts plotting flexural strength against deformability. Using these charts, a "concurrent flexural strength and deformability design" that would allow structural engineers to consider simultaneously both strength and deformability requirements is developed. For application in real construction practice where concrete strength is usually prescribed, a simpler method of determining the maximum and minimum limits of degree of reinforcement for a particular pair of strength and deformability demand is proposed. Numerical examples are presented to illustrate the application of both design methods.

• 대한화학회지
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• 제24권4호
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• pp.322-328
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• 1980

1,3,5,7-Tetranitro-1,3,5,7-tetraazacyclooctane (homocyclonite)의 합성에 있어서 부산물로 1,3,5-trinitro-1,3,5-triazacyclohexane (cyclonite)이 동시에 생성되는데, 본 연구는 반응메카니즘 측면에서 주 생성물 homocyclonite로 가는 중간생성물인 1,5-endomethylene-3,7-dinitro-1,3,5,7-tetraazacyclooctane (DPT)을 액체크로마토그래피로 추적하고 반응계내의 총 메틸렌대 아미노 N 비율을 결정하기 위해 반응중에 첨가되는 paraformaldehyde의 최적의 양을 찾기 위한 이론적인 식을 유도하였으며, (식 1 참조) 실제 실험에서 얻어진 결과와 비교함으로서 주생성물인 homocyclonite의 최대의 수율을 얻기 위한 반응조건을 확인하였다.

• Architectural research
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• 제19권4호
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• pp.117-124
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• 2017

In this research, we carried out finite element analysis depends on the variations such as the strength of the main bar, concrete, shear-span ratio(a/d) and existence of shear reinforcing bar. Throughout the results of FEM analysis, we were able to figure out how each variation can effect on shear performance. As the strength of concrete increased, the maximum shear force enhancement effect of each specimen was evaluated. As a result, the shear strengthening effect was 51~97% for shear reinforced specimens, and 26~44% for non-shear reinforced specimens. As the yield strength of reinforcing bars increases, the shear reinforcement effect of the specimen the specimens without shear reinforcement were 3%~6% higher than those with shear reinforcement. Theoretical and analytical values were compared using the design equations obtained from the CEB code. Theoretical and analytical values were compared using the design equations obtained from the CEB code. As a result, the error rate was the highest at 3.64 in the S1.0-C0 series and the lowest at 1.46 in the S1.7-C1 series. Therefore, the design equation of the CEB code is estimated to underestimate the actual shear strength of deep beams that are not subjected to shear reinforcement.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 1997년도 추계학술발표회논문집(1)
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• pp.599-604
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• 1997

One of important inconsistencies in the six-equation model predictions has been found to be the force experienced by a single bubble placed in a convergent stream of liquid. Various sets of governing equations yield different amount of forces to hold the bubble stationary in a convergent nozzle. By using the first order potential flow theory, it is found that the six-equation model can not be used to estimate the force experienced by a deformed bubble. The theoretical value of the particle stress of a bubble in a convergent nozzle flow has been found to be a function of the Weber number when bubble distortion is allowed. This force has been calculated by using different sets of governing equations and compared with the theoretical value. It is suggested in this study that the bubble size distribution function can be used to remove the presented inconsistency by relating the interfacial variables with different moments of the bubble size distribution function. This study also shows that the inconsistencies in the thermal-hydraulic governing equation can be removed by mechanistic modeling of the phasic interface.

• 한국환경과학회지
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• 제2권2호
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• pp.135-144
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• 1993

The purpose of this experimental research was focused to improve the quality of the effluent and the yielded sludge when the papermill wastewater was treated by the indirect aerated submerged biofilter as a second treatment method of papermill wastewater. Changing the various experimental factors (Nutrient additions or not, HRT, Fh ratio, recirculation ratio, etc) with indirect aerated submerged biofilter, the results are as follows. 1) because of the microbes concentration could be sustained to 9, oho man in submerged biofilter and then the volumetric organic loads could be increased to 2.7 kg-BOD/$m^3$<\TEX>/day, the reactor volume can be reduced. 2) Because of the yield coefficient(Y) and the endogenous decay coefficient(kd) were revealed 0.4 and 0.07/d, the yielded sludge volume was reduced. 3) The concentration of the sloughed sludge in the reactor was 2.62~4.01 %, so the thickener could be omited in the papermill wastewater sludge treatment process. 4) When the operating was conducted at HRT of 4hrs, the treatment efficiencies of BOD and COD were obtained 80% and 70%. 5) The range of the theoretical recirculation ratios of this reactor was 14~26. According to those ratios, at the low loads ( BOD volumetric loads is less than 0.79 kg-BOD/$m^3$<\TEX>/day, FM ratio is less than 2.0/d) the results were fitted to the theoretical recirculation ratios (14 ~26) and at the high loads the efficiency were increased to the rise of recirculation ratios.

• Steel and Composite Structures
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• 제43권2호
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• pp.201-219
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• 2022

This paper firstly proposed high performance composite columns for cold-region infrastructures using ultra-high performance concrete (UHPC) and ultra-high strength steel (UHSS) Q960E. Then, 24 square UHPC-filled UHSS tubes (UHSTCs) at low temperatures of -80, -60, -30, and 30℃ were performed under axial loads. The key influencing parameters on axial compression performance of UHSS were studied, i.e., temperature level and UHSS-tube wall thickness (t). In addition, mechanical properties of Q960E at low temperatures were also studied. Test results revealed low temperatures improved the yield/ultimate strength of Q960E. Axial compression tests on UHSTCs revealed that the dropping environmental temperature increased the compression strength and stiffness, but compromised the ductility of UHSTCs; increasing t significantly increased the strength, stiffness, and ductility of UHSTCs. This study developed numerical and theoretical models to reproduce axial compression performances of UHSTCs at low temperatures. Validations against 24 tests proved that both two methods provided reasonable simulations on axial compression performance of UHSTCs. Finally, simplified theoretical models (STMs) and modified prediction equations in AISC 360, ACI 318, and Eurocode 4 were developed to estimate the axial load capacity of UHSTCs at low temperatures.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2010년도 춘계학술대회 초록집
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• pp.253-253
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• 2010

Rice straw was pretreated with aqueous ammonia in order to enhance enzyme digestibility. Soaking in ammonia aqueous (SAA) was conducted with 15% ammonia, at $60^{\circ}C$. for 24 h. Optimization of both saccharification conditions and enzyme loading of SAA rice straw was carried out. Especially enzyme loading test was performed using statistical method. Moreover proton beam irradiation (PBI) was also performed to overcome the problem which inhibit the enzyme digestibility at 1-25 kGy doses with 45 MeV of beam energy. Optimal condition for enzymatic saccharification was follows; pH 4.8, $50^{\circ}C$, 60 FPU of enzyme activity, 1:4 ratio of celluase and ${\beta}$-glucosidase. Also, optimal doses of PBI on rice straw and SAA-treated rice straw for efficient sugar recovery were found to be 3 kGy, respectively. When saccharification was performed with optimal condition, glucose conversion yield was 89% of theocratical maximum in 48 h, and 3 kGy of PBI was applied to SAA-treated rice straw, approximately 90% of the theoretical glucose yield was obtained in 12 h. The results of X-ray diffractometry (XRD) support the effect of both SAA and PBI on sugar recovery, and scanning electron microscopy (SEM) images unveiled the physical change of the rice straw surface since rugged rice straw surface was observed.