This study was carried out to investigate the in vivo developmental potential of mouse zona-hatched blastocysts (HBs). The HBs were cultured in vitro until day 5 and day 6 from zygotes produced in vivo and classified to small (S-HBs), medium (M-HBs) and large (L-HBs) on the basis of embryo diameters. The results obtained in these experiments were summarized as follows ; 1) when the blastocysts at day 4 were further cultured for $24\sim48hr$, HBs obtained at day 5 and day 6 culture in vitro were 29.1% and 22.8%, respectively. 2) Also, when the total cell number of HBs were counted, cell numbers of classified HBs on day 5 and day 6 to small ($77.3\pm5.3$, $59.6\pm4.4$), medium ($83.7\pm4.0$, $66.8\pm3.5$) and large ($100.7\pm2.6$, $88.9\pm3.8$) were increased as their size increases. Especially, there were significantly different between S-HBs and L-HBs (p<0.01). 3) In addition, when the classified HBs were transferred into when the classified HBs were transferred into day 3 pseudopregnant recipients, the pregnancy and implantation rates of S-HBs (28.6%, 15.7%), M-HBs (44.4%, 30.9%) and L-HBs (62.5%, 49.1%) at day 5 were increased as their size increases. However, this pattern was not showed in embryo transfer of day 6 HBs. But, when the live fetuses formation against total implantation rates were observed, the result (87.5%) of S-HBs of day 5 was significantly higher than that of the others (p<0.01). Therefore, this study demonstrates that in vitro cultured healthy HBs can not only be developed normally with good pregnancy rates, implantation rates and live fetuses formation, but also served as a fundamental data for utility of supernumerary HBs in human blastocyst transfer.
Kim, Kyeong-Min;Shin, Dongjin;Kim, Kyeong-Hoon;Kwon, Yu-Ri;Park, Hyo-Jin;Hyun, Jong-Nae;Kim, Young-Jin;Oh, Myung-Kyu;Park, Young-Hoon
Journal of Plant Biotechnology
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v.45
no.4
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pp.375-381
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2018
The consumption of oats (Avena sativa L.) with high nutritional utility is accelerating due to the increased consumers' demand for functional foods. In Korea, naked oats are used as food, while covered oats are used for animal feed. However, it is difficult to distinguish naked oats from covered oats when the husk is removed from the grains by a special process. The present study was carried out to investigate experimental methods that would be beneficial in the segregation of different types of oats after husk removal. Grain quality-related biochemical compounds were analyzed in a bid to differentiate the oat dehulling characteristics. In addition, 61 SSR markers were examined for genetic relationship and variety identification of oats using five naked and seven covered oat varieties. Results showed that, the contents of protein, lipid, and ${\beta}-glucan$ were not significantly different among the oat varieties and this could not be used as an index for distinguishing oats husk character. However, in the fatty acid composition ratio,, naked oats had a higher ratio of stearic acid (C18:0) and oleic acid (C18:1) than covered oats, and covered oats had a higher ratio of linoleic acid (C18:2) and linoleic acid (C18:3) than naked oats. The assessment of SSR marker genotype revealed that 33 polymorphic bands among 12 oat varieties and 1 variety could be distinguished through the combination of polymorphic markers thus indicating the usability of these markers for variety identification in oats.
Urban is an area where small-scale changes to individual buildings occur frequently. An existing urban building database requires periodic updating to increase its usability. However, there are limitations in data collection for building changes over a wide urban. In this study, we check the possibility of detecting building changes and updating a building database by using satellite images that can capture a wide urban region by a single image. For this purpose, building areas in a satellite image are first extracted by projecting 3D coordinates of building corners available in a building database onto the image. Building areas are then divided into roof and facade areas. By comparing textures of the roof areas projected, building changes such as height change or building removal can be detected. New height values are estimated by adjusting building heights until projected roofs align to actual roofs observed in the image. If the projected image appeared in the image while no building is observed, it corresponds to a demolished building. By checking buildings in the original image whose roofs and facades areas are not projected, new buildings are identified. Based on these results, the building database is updated by the three categories of height update, building deletion, or new building creation. This method was tested with a KOMPSAT-3A image over Incheon Metropolitan City and Incheon building database available in public. Building change detection and building database update was carried out. Updated building corners were then projected to another KOMPSAT-3 image. It was confirmed that building areas projected by updated building information agreed with actual buildings in the image very well. Through this study, the possibility of semi-automatic building change detection and building database update based on single satellite image was confirmed. In the future, follow-up research is needed on technology to enhance computational automation of the proposed method.
The purpose of this study is to analyze the performance of Virtual Desktop Infrastructure(VDI) adoption. VDI performance was measured by IS manager (system quality, security, and managerial operation) and business user (usability, access, and user satisfaction). The survey questionnaires were developed for measuring VDI performance. 84 data samples were collected from the companies that had adopted cloud-based VDI. This research model was verified by Smart-PLS and SPSS. The research findings were as follows: First, the companies using VDI experienced actual performance, but they did not attain their expectation. Second, as results of comparing between IS managers and business users, IS administrators had considerably higher performance than business users, which indicates that there were big differences in performance perception among users. Compared with prior research such as technical trend, system construction, and performance improvement, this study has the following implications. First, by comparing the expected performance with the actual performance of the companies that have implemented and operating VDI, it was suggested how a company that wants to adopt VDI can manage the expectation level of VDI and achieve higher actual performance. Second, because the perception of VDI performance differs between business users and system managers, it is meaningful that a fair evaluation of VDI performance requires a balanced consideration of business users and system managers.
Creativity is a important keyword for users as well as for main design organization who needs it. But little attention has been given to the aspect of user's creativity, also there has been a few attempt to apply it into design development until now. Nowadays in design areas, user's experiences and actions are changing the passive states receiving meanings into the active states creating meanings voluntarily. It is resonable to suppose that creative stage is important for users and they have the possibility of new ideas of uses and creating new productions. User's experiences of objects includes that of being formed or supported previously and that of voluntary interpretations acquired for himself, which it may be the possibilities predicted in design process or unknown user's action areas. It is likely that creative use process by themselves are the actions applied and deviated from usability and function by main design organization, also creative productions are arranged and made by users. These have a scope of examination and research in probability that is occurs frequently in user. In this research, approaching with a term, 'User gestures', User gestures are the characteristic action areas based on user's voluntary behaviors, where are revealed a unessential and non-operational function as a action itself and various transformation and creation of products as a outcome of action. This fact proves clearly that user gestures have a worth of alive spectrum to observe aspects of user culture and could be a attractive approach to seek easily new design concept for designer and developer. A further direction of this study will be following areas, Ethnography methods research of user gestures, Cultural research to phenomenon of user design and UGSBD(User gesture scenario based design) research. And it seems probable that they are applied in design development as follows, User initiative customization products, User participatory recycling products and creativity-experience design.
To reveal the possibility as a source of functional food and to increase the usability of mulberry fruits, amino acid analysis with mulberry fruits was carried out with L-8800 Amino acid auto analyzer. We analyzed 16 amino acid components including 6 essential amino acids. According to the results, the mean content of total amino acid was 4,640.8 mg/100 g DW. 'Sabangso(II)' showed the highest total amino acid content (6,919.3 mg/100 g DW) among the fifty accessions tested, whereas 'Gukkwang' showed the lowest content (3,389.1 mg/100 g DW). The distribution rate of amino acid components decreased in the order of Glu ($20.1\%$) > Gly ($12.7\%$) > Asp ($12.5\%$) > Thr ($8.2\%$) > Ala ($7.9\%$) > Arg ($7.5\%$) > Pro ($6.1\%$) > Ser ($6.1\%$) > Val ($5.7\%$) > Leu ($5.3\%$) > Ile ($2.7\%$) > His ($2.5\%$) > Met ($0.9\%$) > Tyr ($0.8\%$) > Lys ($0.7\%$) > Cys ($0.3\%$). 'Sabangso(II)' showed the highest content in 11 amino acid components, which were Glu (1,293.2 mg/100 g), Ser (390.2 mg/100 g), Thr (564.4 mg/100 g), His (175.3 mg/100 g), Arg (590.4 mg/100 g), Lys (53.5 mg/100 g), Gly (589.4 mg/100 g), Ala (499.3 mg/100 g), Leu (481.9 mg/100 g), Ile (240.9 mg/100 g), and Val (407.6 mg/100 g). Tyr and Cys were the highest in 'Jangsosang' (70.8 mg/100 g, 19.3 mg/100 g, respectively), and Asp, Pro, and Met were the highest in 'Jasan(I)', 'Hwansipchosaeng' and 'Palcheongsipyung(I)' (1,098.8 mg/100 g, 364.4 mg/100 g, 68.8 mg/100 g, respectively).
KIPS Transactions on Software and Data Engineering
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v.2
no.8
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pp.555-564
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2013
Innovative evolution in mobile devices along with recent spread of Tablet PCs and Smart Phones makes a new change not only in individual life but also in enterprise applications. Especially, in the case of medium-large mobile applications for large enterprises which generally takes more than 3 months of development periods, importance and complexity increase significantly. Generally Agile-methodology is used for a development process for the medium-large scale mobile applications, but some issues arise such as high dependency on skilled developers and lack of detail development directives. In this paper, S-MADP (Smart Mobile Application Development Process) is proposed to mitigate these issues. S-MADP is a service oriented development process extending a object-oriented development process, for medium-large scale mobile applications. S-MADP provides detail development directives for each activities during the entire process for defining services as server-based or client-based and providing the way of reuse of services. Also, in order to support various user interfaces, S-MADP provides detail UI development directives. To evaluate the performance of S-MADP, three mobile application development projects were conducted and the results were analyzed. The projects are 'TBS(TB Mobile Service) 3.0' in TB company, mobile app-store in TS company, and mobile groupware in TG group. As a result of the projects, S-MADP accounts for more detailed design information about 'Minimizing the use of resources', 'Service-based designing' and 'User interface optimized for mobile devices' which are needed to be largely considered for mobile application development environment when we compare with existing Agile-methodology. Therefore, it improves the usability, maintainability, efficiency of developed mobile applications. Through field tests, it is observed that S-MADP outperforms about 25% than a Agile-methodology in the aspect of the required man-month for developing a medium-large mobile application.
Purpose: Onco flash shortens a scan time with half and there is a possibility of getting the data which corresponds in existing. The experiment which makes the image whose Onco Flash is excellent OSEM tried, as changes parameter of time, iteration. After reconstituting an image, produces FWHM and executes an evaluation. Materials and Methods: Siemens e.cam gamma camera, standard Jaszczak phantom and spatial resolution phantom was used. In order for the bubble not to enter, implants 2 mCi and volume 0.25 cc $^{99m}Tc$ respectively in line 3 to spatial resolution phantom. Put on that phantom on the table correctly, and acquires an image. 15 mCi putting in distilled water to mix $^{99m}Tc$ well in Jaszczak phantom and acquires image just like spatial resolution phantom. Reconstructs and converts the image to digital image as Sante program. Produce FWHM and evaluate by Amide. Results: The non-scattered image shows better FWHM value than scattered image. As time increases from 10 sec to 30 sec for 5sec interval, FWHM appeared to 30.1, 28.5, 24.5, 23.6, 23.4 mm. At the standard iteration value 4, OSEM FWHM shows 8.0 mm, and Onco Flash is 8.1 mm. As fade in iteration, FWHM value more and more decreased. Conclusion: When using Onco Flash, shortens a scan time, and enhances image quality. Also, user can adjust the parameters to improve resolution. Therefore, patient and user are satisfied with these merits.
Kim, M.K.;Kim, E.Y.;Yi, B.K.;Yoon, S.H.;Park, S.P.;Chung, K.S.;Lim, J.H.
Clinical and Experimental Reproductive Medicine
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v.24
no.3
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pp.347-353
/
1997
This study was to test whether in vitro/in vivo survival of vitrified mouse blastocysts was influenced by culture conditions and ET method. Mouse blastocysts were obtained from in vitro fertilization and cultured for 4 days in M16 medium, and they were vitrified in EFS40 which contained 40% ethlyene glycol, 18% Ficoll and 0.5 mol sucrose in PBS. In experiment I, in vitro and in vivo survival rate of these embryos were evaluated in different culture condition after thawing. When thawed embryos were cultured in M16 medium as a control, m-CR1 medium contained 20 amino acids (2% BME amino acis and 1% MEM non-essential amino acids solution) and 4 mg/ml BSA and cumulus monolayer cell co-cultured condition in mCR1 medium (10% FBS), their in vitro survival at 24 hr after thawing was not affected by culture condition (75.6, 83.1, 82.4%). However, in vivo survival rates of implantation in m-CR1 medium (80.4%) were significantly higher than those of M16 medium (51.2%), co-culture (57.1%) condition, although there was no difference in live fetuses rates on day 15 gestation (39.0, 49.0, 38.1%). In experiment II, the in vivo development potential of embryos by ET methods was examined. When blastocysts were transferred to the day 2, 3 pseudopregnant recipient without culture soon after thawing, no pregnant recipient was obtained on the day 2 pseudopregnancy, and 50% of pregnancy rates and 15.4% of live fetus rates were obtained on the day 3 pseudopregnant recipients. These results were significantly lower than those of transferred group (day 3 pseudopregnant recipients) after culture for 16 hr post thawing (73.5, 57.1%) (p<0.05). In experiment III, to elevate usability of delayed embryos in vitro/in vivo survival of vitrified embryos (day 4 early, day 5 early and expanding blastocyst) were examined. in vivo survival rates (live fetus, total implantation) were higher in day 4 early blastocysts (33.3, 66.7%) than in day 5 expanding blastocysts (29.0, 38.7%), although the highest in vitro survival rates were obtained in the day 5 expanding brastocysts (78.3%). Therefore, these results suggest that the in vitro/in vivo survival rates of vitrified embryos could be improve by the culture condition and ET method and that the in vivo development rates of delayed embryos were decreased with longer culture duration in vitro. It means that more effective cryopreservation was obtained in day 4 early blastocysts than in day 5 expanding blastocysts.
This study was conducted to provide information on local resource plants by identifying aquatic and Riparian flora. We investigated the aquatic and riparian floras in 3 streams(Byeongseong-cheon, Buk-cheon, Oeseo-cheon) from February to October 2019. 321 taxa (i.e. 300 species, 5 subspecies, 15 varieties 1 Cultivars from 203 genera of 78 families) of the vascular plants were found in the survey area. Byeongseong-cheon is 133 taxa, Buk-cheon is 233 taxa and Oeseo-cheon is 132 taxa. Among 321 taxa, we found 5 endemic species, 3 red list plants, and However, endangered plants were not found in 3 streams. Aquatic and Riparian plant 138 taxa(i.e. Aquatic plant 20 taxa, Riparian plant 118 taxa). Life forms is annual plant 43 taxa, biennial plant 24 taxa, perennial plant 71 taxa. Aquatic plant growth forms emergent hydrophyte 13 taxa, floating leaved hydrophyte 1 taxa, submerged hydrophyte 6 taxa. The number of floristic regional indicator plants was 15 (i.e. 1 species of IV degree, 3 taxa of III degree, 5 taxa of II degree, and 6 taxa of I degree). Approved foreign export plants 31 taxa. In addition, 52 naturalized plants were identified, and the percentage of Naturalized Index (NI) and Urbanization Index (UI) were 16.1%, and 16.2%, respectively. Vascular plant usability and reclassification result is Edible 213 species (66%), Medicinal 244 species (76%), Flavor 10 species (3%), Industrial 136 species (42%), Ornamental 137 species (36%), Restoration 117 species (36%), Compost 155 species (48%), Unknown 7 species (5%). We hope that our results provide reference data to set up strategy of resources plants, conservation of biodiversity in the 3 streams and Sangju-si areas.
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