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Characteristics of Methanol Production Derived from Methane Oxidation by Inhibiting Methanol Dehydrogenase (메탄올탈수소효소 저해시 메탄산화에 의한 메탄올 전환생성 특성)

  • Yoo, Yeon-Sun;Han, Ji-Sun;Ahn, Chang-Min;Min, Dong-Hee;Mo, Woo-Jong;Yoon, Soon-Uk;Lee, Jong-Gyu;Lee, Jong-Yeon;Kim, Chang-Gyun
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.9
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    • pp.662-669
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    • 2011
  • This study was conducted to biologically convert methane into methanol. Methane contained in biogas was bio-catalytically oxidized by methane monooxygenase (MMO) of methanotrophs, while methanol conversion was observed by inhibiting methanol dehydrogenase (MDH) using MDH activity inhibitors such as phosphate, NaCl, $NH_4Cl$, and EDTA. The degree of methane oxidation by methanotrophs was the most highly accomplished as 0.56 mmol for the condition at $35^{\circ}C$ and pH 7 under 0.4 (v/v%) of biogas ($CH_4$ 50%, $CO_2$ 50%) / Air ratio. By the inhibition of 40 mM of phosphate, 50 mM of NaCl, 40 mM of $NH_4Cl$ and $150{\mu}m$ of EDTA, methane oxidation rate could achieve more than 80% regardless of type of inhibitors. In the meantime, addition of 40 mM of phosphate, 100 mM of NaCl, 40 mM of $NH_4Cl$ and $50{\mu}m$ of EDTA each led to generating the highest amount of methanol, i.e, 0.71, 0.60, 0.66, and 0.66 mmol when 1.3, 0.67, 0.74, and 1.3 mmol of methane was each concurrently consumed. At that time, methanol conversion rate was 54.7, 89.9, 89.6, and 47.8% respectively, and maximum methanol production rate was $7.4{\mu}mol/mg{\cdot}h$. From this, it was decided that the methanol production could be maximized as 89.9% when MDH activity was specifically inhibited into the typical level of 35% for the inhibitor of concern.

Eating patterns and use of nutritional information in breast cancer survivors treated with radiation therapy in South Korea (일반인과 유방암 환자간의 식행동 및 영양정보에 관한 인식조사)

  • Kim, Kyoung-Ok;Park, Hyunjin;Chun, Mison;Lee, Eun Hyun;Kim, Hyun-Sook
    • Journal of Nutrition and Health
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    • v.46 no.3
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    • pp.250-260
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    • 2013
  • The purposes of this study were 1) to investigate eating behaviors and patterns in breast cancer patients using a newly developed food frequency questionnaire and 2) to examine perception and use of nutritional information about breast cancer treatment among cancer patients treated with radiation therapy. Sixty breast cancer patients (case group) undergoing radiation therapy in Ajou University Hospital, Suwon, South Korea and 79 healthy women (control group) participated in this study. Mean age of subjects in the control group was $46.00{\pm}7.88$ years and BMI was $23.12{\pm}2.85kg/m^2$, and that of the case group was $50.06{\pm}11.64$ years and $22.32{\pm}3.24kg/m^2$. The results of eating behaviors showed several significant differences between control and case groups. Breast cancer patients ate meals on a more regular basis, on time, and more frequently compared to control subjects. In addition, they preferred more salty or spicy and bland food compared to healthy women. According to answers from the food frequency questionnaire, breast cancer patients consumed significantly lower amounts of boiled white rice, meats and processed food, fish and shellfish, coffee, milk, and cheese, whereas they consumed a significantly large amount of boiled multigrain rice, vegetable, seaweeds, soybean and processed food, and yoghurt compared to healthy women. This study also observed the way in which cancer patients and healthy control subjects obtain information about breast cancer treatment and its reliabilities. Results showed that healthy women did not hesitate to obtain information from mass media, while breast cancer patients would obtain nutritional information from specialists rather than mass media. Results of this survey confirmed that breast cancer patients avoided intake of red meat protein, even though they already recognized the importance of dietary protein intake for recuperation and treatment of the disease. These results could be used for future diet and nutrition guidelines for breast cancer patients.

Effects of Grape Seed Oil Additives on Quality Characteristics of Pressed Ham (포도씨유 첨가가 프레스햄의 이화학적 및 조직감에 미치는 영향)

  • Lee, Jeong-Ill;Yang, Han-Sul;Jeong, Jin-Yeon;Moon, Sang-Hoon;Kim, Cap-Don;Lee, Jin-Woo;Jung, Jae-Doo;Ha, Young-Joo;Kwack, Suk-Chun;Park, Jeong-Suk
    • Food Science of Animal Resources
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    • v.29 no.2
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    • pp.178-187
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    • 2009
  • This study was carried out to investigate the effects of grape seed oil on quality characteristics of pressed ham. Five different treatments were tested based on differences in the amount of grape seed oil added to the pressed ham. As a control, 10% back fat was added without any grape seed oil. For the first treatment, 10% grape seed oil replaced a portion of the lard component added to the pressed ham. For the 2nd, 3rd and 4th treatments, 20%, 30% and 40% of grape seed oil were substituted for lard, respectively. Pressed ham manufactured with grape seed oil was vacuum packaged and stored for 1, 7, 14,21 and 28 d at $4^{\circ}C$. Samples were analyzed for chemical composition, physico-chemical properties (pH, color) and texture characteristics. Typical chemical composition characteristics (crude protein, crude fat, crude ash) were not significantly different between control and grape seed oil treatment groups. Moisture content of grape seed oil treatment groups (GSO 30% and 40%) was significantly lower than that of controls (p<0.05). There was a not clear difference in pH between control and grape seed oil treatment groups. In the 21 d of storage, pH values of all treatments were significantly higher than those of other storage days. Meat color $(CIEL^*\;and\;b^*)$ of grape seed oil treatment group (GSO 40%) was significantly higher than that of control. Whereas meat color a value of GSO 40% treatment was significantly lower than that of control. It was not clearly changed as storage time increased. There was no significant difference in texture between control and grape seed oil treatment groups, and appeared to be unaffected by storage length. Based on these findings, we conclude that the chemical composition and texture characteristics of manufactured pressed ham were not affected by grape seed oil addition. These results also indicate that high-quality pressed ham can be manufactured with increased polyunsaturated fatty acid content.

Predicting N2O Emission from Upland Cultivated with Pepper through Related Soil Parameters (온실가스 배출 파라메타를 이용한 고추밭 토양의 N2O 배출 예측)

  • Kim, Gun-Yeob;Song, Beom-Heon;Hyun, Byung-Keun;Shim, Kyo-Moon;Lee, Jeong-Taek;Lee, Jong-Sik;Kim, Won-Il;Shin, Joung-Du
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.5
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    • pp.253-258
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    • 2006
  • An empirical model of nitrous oxide emission from agricultural soil has been applied. It is based on the relationship between $N_2O$ and three soil parameters, soil mineral N(ammonium plus nitrate) content in the topsoil(0-15cm), soil water-field pore space, and soil temperature, determined in a study on clay loam and sandy loam at the pepper field in 2004. For comparisons between estimated and observed values of $N_2O$ emissions in the pepper field, it was investigated that $N_2O$ amount in the clay loam and sandy loam were overestimated as 12.2% and less estimated as 30%, respectively. However, $N_2O$ emissions were overestimated as 27.1% in the clay loam and 14.7% in the sandy loam from $N_2O$ gas samples collected once a week at the same time analyzing soil parameters. This modelling approach, based as it is well established and widely used soil measurements, has the potential to provide flux estimates from a much wider range of agricultural sites than would be possible by direct measurement of $N_2O$ emissions.

Studies on the Volatile Flavor Compounds of Sesame Oils with Roasting Temperature (볶음온도에 따른 참기름의 휘발성향기성분 변화)

  • Kim, Hyeon-Wee;Park, Ki-Moon;Choi, Chun-Un
    • Korean Journal of Food Science and Technology
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    • v.32 no.2
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    • pp.238-245
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    • 2000
  • This study was investigated to compare the changes of flavors in sesame oil with roasting temperature $(110^{\circ}C{\sim}230^{\circ}C)$. In the results of analyzing the volatile flavor compounds of sesame oil with GC and GC/MS, 26 pyrazines, 11 pyridines, 9 thiazoles, 6 furans, 8 pyrroles, 5 phenols, 8 aldehydes, 8 hydrocarbons, 7 alcohols, 2 indoles, 3 ketones, 10 acids, 4 nitriles, 7 esters, and 5 others were isolated, identified, and quantified. The total amount of flavor compounds was increased with roasting temperature. Detected flavors could be devided into top(peak No. $1{\sim}91$), middle$(92{\sim}197)$ and last note$(198{\sim}224)$ by rentention time. The top notes(initial content 19.87 ppm) which contain pyrazines and provide representative roasted flavors were increased significantly with roasting temperature. Initial content of middle note(17.72 ppm) was increased to 36.71 ppm at $170^{\circ}C$, to 95.61 ppm at $220^{\circ}C$, and to 138.62 ppm at $230^{\circ}C$. Last note was almost unchanged up to $170^{\circ}C$ and increased at $190^{\circ}C$, whereas it indicated a tendency to decrease at $230^{\circ}C$. Pyrazines such as methylpyrazine, 2,5-dimethylpyrazine, 2,6-dimethylpyrazine, trimethylpyrazine, 2-ethyl-3,5-dimethylpyrazine which indicate the major components among volatile flavors were increased slightly up to $150^{\circ}C$ and revealed the higher increase than any other components above $170^{\circ}C$. This tendency was also similar to pyridines, thiazoles, and furans. Most of these compounds are assumed to be developed by thermochemical reactions of sesame components by roasting above $170^{\circ}C$. It seemed that a lot of increase in phenols above $210^{\circ}C$ resulted from the production of guaiacol. Acids were almost unchanged up to $190^{\circ}C$, increased at $210^{\circ}C$, and then decreased above $220^{\circ}C$. It seemed to be resulted from pyrolysis of free fatty acids formed from thermal oxidation of oil.

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Nematodes Associated with Rice in Korea I. Survey on White Tip Nematode (Aphelenchoides besseyi) Damage to Rice and Detection of the Nematode in Rice Seed (한국(韓國)에 있어서 벼기생선충에 관(關)한 연구(硏究) I. 벼이삭선충의 피해율(被害率) 및 침종(浸種)에 의한 선충분리율 조사(調査))

  • Choi, Y.E.;Choi, D.R.;Choi, Y.S.
    • Korean journal of applied entomology
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    • v.25 no.3 s.68
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    • pp.159-167
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    • 1986
  • White tip nematode damage to rice was surveyed at 111 $My{\check{o}}n$ from 36 Gun in Kyongsangnam-Do, $Ky{\check{o}}ngsangbuk-Do\;and\;Ch{\check{o}}llanam-Do$. Rate of damaged stem by the nematode was highest at $Ch'angny{\check{o}}ng-Gun\;in\;Ky{\check{o}}ngsangnam-Do$ as 28.3%, $Y{\check{o}}ngch'on-Gun\;in\;Ky{\check{o}}ngsangbuk-Do$ as 69.3% and $Pos{\check{o}}ng-Gun\;in\;Ch{\check{o}}llanam-Do$ as 40.6%. In pot experiment, no white tip symptoms were appeared in Tongil hybrids except $W{\check{o}}np'ung$ by 3.4% but a great number of the nematode was detected in seeds while symptom and a fair amount of the nematode were observed in Japonica cultivars. The number of nematode in Shingwang cultivar of Tongil hybrids and Dongin cultivar of Japonica was highest by 1997, by 1023 individuals per 10g of seed respectively. Detection rate of the nematode was different depended upon peeling of the husks. Less than 50% was detected from unhulling seed compared with hulled seed except 8 cultivars such as $Shins{\check{o}}nchal$, Hangangchal, $S{\check{o}}gwang,\;Y{\check{o}}ngpung$, Milyang 23, $Such{\check{o}}ng,\;Taech'{\check{o}}ng\;and\;S{\check{o}}nam$. More effective detection was oftained with temperature level at $25^{\circ}C$ and increased with immersing time regardless of peeling the husks.

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Study on Pretreatment Methods to Prevent Tissue Softening of Heated Onion (가열 양파의 조직 연화 방지를 위한 전처리 방법에 관한 연구)

  • Choi, Jun-Bong;Cho, Won-Il
    • Korean Journal of Food Science and Technology
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    • v.47 no.1
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    • pp.56-62
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    • 2015
  • Various pretreatment methods were evaluated to prevent tissue softening of heated onion. Changes in onion tissue firmness during heating were explained by 3-mechanism model consisting of texture hardening at low temperature ($60-80^{\circ}C$) and substrate softening at high temperature. Preheating of onion in a $Ca^{2+}$-containing solution significantly improved its texture after high-temperature heating. The improvement of firmness by preheating at low temperature was related to the formation of strong cross-linking between carboxyl groups and $Ca^{2+}$ by the action of pectin methylesterase in onion. The highest firmness was obtained by pre-heating at $70^{\circ}C$ for 120 min in 0.5% calcium solution. This result was supported by chemical analysis showing that the amount of bound calcium was the highest at $70^{\circ}C$. Further investigation should be carried out to establish the optimal conditions to prevent the softening of various vegetables.

Effect of Cover Crop Hairy Vetch on Prevention of Soil Erosion and Reduction of Nitrogen Fertilization in Sloped Upland (경사지 밭토양 유실억제 및 질소비료절감에 대한 피복작물 헤어리벳치의 효과)

  • Seo, Jong-Ho;Park, Jong-Yeol;Song, Duk-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.3
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    • pp.134-141
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    • 2005
  • Cultivation of winter cover crops and its soil utilization for summer main crops in slope upland is very important at the respects of diminution of soil erosion and non-point pollution source. The effects of cover crop, hairy vetch as no-tillage or conventional tillage on prevention of soil erosion and reduction of chemical nitrogen fertilization were investigated in the slope upland with whiter clover living mulch (partial tillage) in Suwon for three years and with rye (conventional tillage) in Hongcheon for two years, respectively. In Suwon, amounts of soil lost by rainfall runoff decreased as much as 90% by hairy vetch-no tillage (HV-NT), white clover-partial tillage (WF-PT) together with the decrease of rainfall runoff compared to winter fallow with conventional tillage (WF-CT). In addition, amounts of weed also decreased as much as 80-90% by HV-NT and WF-PT. Corn yield decreased much at the plot of WF-PT mainly due to competition for soil water and nutrients between clover and corn at the early corn growth stage. On the contrary, corn yield increased by HV-NT compared to WF-CT regardless of weed control. In Hongcheon, amounts of soil eroded during winter season before corn seeding were reduced as much as 95% by cultivation of hairy vetch and rye compared to winter fallow. Amount of soil eroded during waxy corn growing season was reduced as much as 98% by HV-NT compared to WF-CT. Also, soil incorporation of hairy vetch and rye as green manure with conventional tillage at corn seeding time could reduce soil erosion as much as 70% compared to no soil cover with conventional tillage. Ear yields of waxy corn were increased 10% higher at hairy vetch green manure (HV-CT) without nitrogen fertilizer, 20% higher at HV-NT with standard nitrogen fertilizer, respectively than WF-CT. But ear yields of waxy corn were decreased by rye green manure (R-CT) and HV-NT at the condition of no nitrogen fertilizer. It was concluded that hairy vetch was better as winter cover crop to reduce both soil erosion and chemical nitrogen fertilizer simultaneously in slope upland than other cover crops.

Simultaneous Determination of 8 Preservatives (6 Parabens, 2-Phenoxyethanol, and Chlorphenesin) in Cosmetics by $UPLC^{TM}$ ($UPLC^{TM}$를 이용한 화장품 중 보존제 8종(파라벤 6종, 페녹시에탄올, 클로페네신)의 동시분석)

  • Park, Jeong-Eun;Lee, So-Mi;Jeong, Hye-Jin;Chang, Ih-Seop
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.33 no.4
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    • pp.263-267
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    • 2007
  • Parabens are used in nearly all types of cosmetics and toiletries because they are formulated well and have broad spectrum of activity, interness, low costs and excellent chemical stability in relation to pH. 2-phenoxyethanol and chlorphenesin are common preservatives which are usually used in combination with parabens in cosmetics. Toxicity of parabens is generally low but application of parabens to damaged or broken skin has resulted in sensitization. Moreover, the possibility of their estrogenic potential, anesthetic effects and reproductive toxicity has been reported. Consequently there are some regulations in use of parabens. And the maximum permitted concentrations of chlorphenesin and 2-phenoxyethanol in cosmetic products are authorized by the same reasons. So it is important to control and estimate the amount of parabens in products. In this article, we proposed a valid method for the simultaneous determination of 8 preservatives including parabens in a short time using ultra performance liquid $chromatography^{TM}\;(UPLC^{TM})$. Separation of eight components was achieved in less than 10 min and resolutions were reasonable (USP resolution ${\geqq}\;2$). And limit of detection and quantification were evaluated. The method was suitably validated for specificity, linearity, precision (repeatability, intermediate precision) and accuracy for assay (recovery) based on International conference on harmonisation (ICH) guideline. The method was applicable to analysis of preservatives in cosmetic products.

Study on Evaluation of Carbon Emission and Sequestration in Pear Orchard (배 재배지 단위의 탄소 배출량 및 흡수량 평가 연구)

  • Suh, Sanguk;Choi, Eunjung;Jeong, Hyuncheol;Lee, Jongsik;Kim, Gunyeob;Sho, Kyuho;Lee, Jaeseok
    • Korean Journal of Environmental Biology
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    • v.34 no.4
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    • pp.257-263
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    • 2016
  • Objective of this study was to evaluate the carbon budget on 40 years old pear orchard at Naju. For carbon budget assessment, we measured the soil respiration, net ecosystem productivity of herbs, pear biomass and net ecosystem exchange. In 2015, pear orchard released about $25.6ton\;CO_2\;ha^{-1}$ by soil respiration. And $27.9ton\;CO_2\;ha^{-1}$ was sequestrated by biomass growth. Also about $12.6ton\;CO_2\;ha^{-1}$ was stored at pruning branches and about $5.2ton\;CO_2\;ha^{-1}$ for photosynthesis of herbs. As a result, 25.6 ton of $CO_2$ per ha is annually released to atmosphere. At the same time about 45.7 ton of $CO_2$ was sequestrated from atmosphere. When it sum up the amount of $CO_2$ release and sequestration, approximately $20.1ton\;CO_2\;ha^{-1}$ was sequestrated by pear orchard in 2015, and it showed no significant differences with net ecosystem exchanges ($17.8ton\;CO_2\;ha^{-1}\;yr^{-1}$) by eddy covariance method with the same period. Continuous research using various techniques will help the understanding of $CO_2$ dynamics in agroecosystem and it can be able to present a new methodology for assessment of carbon budget in woody crop field. Futhermore, it is expected that the this study can be used as the basic data to be recognized as a carbon sink.