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Use of Protease Produced by Bacillus sp. SJ-121 for Improvement of Dyeing Quality in Wool and Silk

  • Kim Soo-Jin;Cha Min-Kyoung;Oh Eun Taex;Kang Sang-Mo;So Jae-Seong;Kwon Yoon-Jung
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.3
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    • pp.186-191
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    • 2005
  • In this study, a microorganism-produced protease was used to improve the quality of fabrics. First, the protease-producing bacteria were isolated from soils, and one of them was selected and identified as Bacillus sp. SJ-121. The optimal medium composition for its growth and protease production was determined to be as follows: glucose 1g/L, soybean meal 0.5g/L, soy peptone 0.5, $K_2HPO_4\;0.2,\;MgSO_4\cdot7H_2O\; 0.002,\;NaCl\;0.002,\;and\;Na_2CO_3g/L$. Also, the optimal temperature for the production of the protease by Bacillus sp. SJ-121 was about $40^{\circ}C$ at pH 7. The wool and silk were treated with the protease from Bacillus sp. SJ-121. Following the protease treatment, changes in the surface of a single yarn of the fabrics were observed by both an optical microscope and a scanning electron microscope (SEM). Changes in the K/S value of the wool and silk were measured by spectrophotometric analysis, in order to determine the amount of dye uptake in the fabrics. We also performed a tensile strength examination in order to determine the degree and nature of mechanical changes in single yarns of the wool and silk fabrics. By increasing the protease treatment time to 48 h, the dyeing characteristics of the fabrics were enhanced, and the surfaces of the single yarns of the fabrics became smoother, due to the removal of soil and scale in them. However, no mechanical changes were detected in the fabrics. Therefore, we suggest that proper treatment of the protease produced by Bacillus sp. can improve the quality of silk and wool.

Thermo-fluid engineering in deep geothermal energy

  • Kim, Yeong-Won
    • Proceedings of the Korean Vacuum Society Conference
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    • 2015.08a
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    • pp.84.1-84.1
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    • 2015
  • Recent years in particular in Korea see intensive interests in a deep geothermal engineering and its application in different uses as far as from direct uses to power generation sectors, that are achieved by harnessing hot energy sources from the earth. For instance widespread interest has been generated because the geothermal energy is the source that one extracts it for more than 20 hours per day and for about 30 years of an operation of the plant, which enables to give base load as for heating as well as an electric generation. In retrospect, shallow geothermal energy using heat pumps is commonplace in Korea while the deep geothermal is in the early stage of the development. Geothermal energies in view of the way of extracting heat are mainly categorized into several types such as a single well system, a hydrothermal system, an enhanced geothermal system (EGS) etc. In this talk, this speaker focuses on the thermo-fluid engineering of the single well system by introducing the modeling in order to harness hot fluid that is thermally balanced with the fluid of an injection well, which provides a challenge to assess the life time of the well. To avoid the loss of the temperature in producing the hot fluid, a specialized pipe or a borehole heat exchanger has been designed, and its concept is introduced. On the other hand, a binary system or an organic Rankine cycle, which provides the methodology to convert the heat into an electricity, is briefly introduced. Some experimental results of the binary system which has been constructed in our lab will be presented. Lastly as for the future direction, some comments for the industrialization of the deep geothermal energy in this country will be discussed.

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Long-Tail Watchdog Timer for High Availability on STM32F4-Based Real-Time Embedded Systems (STM32F4 기반의 실시간 임베디드 시스템의 가동시간 향상을 위한 긴 꼬리 와치독 타이머 기법)

  • Choi, Hayeon;Yun, Jiwan;Park, Seoyeon;Kim, Yesol;Park, Sangsoo
    • Journal of Korea Multimedia Society
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    • v.18 no.6
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    • pp.723-733
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    • 2015
  • High availability is of utmost importance in real-time embedded systems. Temporary failures due to software or hardware faults should not result in a system crash. To achieve high availability, embedded systems typically use a combination of hardware and software techniques. A watchdog timer is a hardware component in embedded microprocessors that can be used to automatically reset the processor if software anomalies are detected. The embedded system relies on a single watchdog timer, however, can be permanently disabled if the timer is not properly configured, e.g. falling into an indefinite loop. STM32F4 provides two different types of watchdog timer in terms of timing accuracy and robustness. In this paper, we propose a hybrid approach, called long-tail watchdog timer, to utilize both timers to achieve self-reliance in embedded systems even though one of timers fails. Experimental results confirm that the proposed approach successfully handles various failure scenarios and present performance comparisons between single watchdog timer and hybrid approach in terms of configuration parameters of watchdog timers in STM32F4, counter value and window size.

Comparative Study of the Latissimus Dorsi and Gracilis Muscle Reconstruction for Reanimation of a Paralyzed Face (안면 마비의 재건에서 광배근 유리피판과 박근 유리피판의 비교 연구)

  • Kang, Dong Hee;Lim, Chan Su;Koo, Sang Hwan;Park, Seung Ha
    • Archives of Plastic Surgery
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    • v.34 no.3
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    • pp.336-341
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    • 2007
  • Purpose: The most accepted method for the reanimation of a paralyzed face is the two-stage method that combines cross-face nerve grafting with free-muscle transfer. Although the results of reconstruction with this method are satisfactory, there is an excessive delay between the stages, which prolongs the period of rehabilitation. In order to overcome this drawback, a one- stage, neurovascular free-flap reconstruction method using free neurovascular muscle flaps is introduced. Methods: From 1994 to 2004, 35 patients with longstanding facial palsy were treated. Fifteen patients underwent the single-stage reconstruction with the latissimus dorsi muscle, and 20 patients underwent the two-stage reconstruction method with the gracilis muscle. We compared the long-term results of the two methods of reconstruction. The mean follow-up period was 28.7 months for one-stage reconstruction, and 35.2 months for the two-stage, respectively. Results: In the patient group of the single stage reconstruction, both mouth corner excursion and animation grade were markedly improved at the final postoperative visit. Moreover, the first muscle contraction occurred earlier in this group, than in the two-stage reconstruction group. However, four patients in the single stage group never achieved a first muscle contraction or mouth corner excursion. Conclusion: Facial palsy is a very challenging condition for cosmetic surgeons to deal with. Traditional methods for treatment of chronic facial palsy use a two-stage muscle flap which is time-consuming and burdensome to patients, many of whom are averse to waiting 8 to 12 months to complete the two stages. The one-stage reconstruction method described herein uses a latissimus dorsi free-flap and has demonstrated consistent positive outcomes in clinical assessments.

Performance Analysis of Opportunistic Spectrum Access Protocol for Multi-Channel Cognitive Radio Networks

  • Kim, Kyung Jae;Kwak, Kyung Sup;Choi, Bong Dae
    • Journal of Communications and Networks
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    • v.15 no.1
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    • pp.77-86
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    • 2013
  • Cognitive radio (CR) has emerged as one of effective methods to enhance the utilization of existing radio spectrum. Main principle of CR is that secondary users (SUs) are allowed to use the spectrum unused by primary users (PUs) without interfering PU's transmissions. In this paper, PUs operate on a slot-by-slot basis and SUs try to exploit the slots unused by PUs. We propose OSA protocols in the single channel and we propose an opportunistic spectrum access (OSA) protocols in the multi-channel cognitive radio networks with one control channel and several licensed channels where a slot is divided into contention phase and transmission phase. A slot is divided into reporting phase, contention phase and transmission phase. The reporting phase plays a role of finding idle channels unused by PUs and the contention phase plays a role of selecting a SU who will send packets in the data transmission phase. One SU is selected by carrier sense multiple access / collision avoidance (CSMA/CA) with request to send / clear to send (RTS/CTS) mechanism on control channel and the SU is allowed to occupy all remaining part of all idle channels during the current slot. For mathematical analysis, first we deal with the single-channel case and we model the proposed OSA media access control (MAC) protocol by three-dimensional discrete time Markov chain (DTMC) whose one-step transition probability matrix has a special structure so as to apply the censored Markov chain method to obtain the steady state distribution.We obtain the throughput and the distribution of access delay. Next we deal with the multi-channel case and obtain the throughput and the distribution of access delay by using results of single-channel case. In numerical results, our mathematical analysis is verified by simulations and we give numerical results on throughput and access delay of the proposed MAC protocol. Finally, we find the maximum allowable number of SUs satisfying the requirements on throughput and access delay.

Multi-Style License Plate Recognition System using K-Nearest Neighbors

  • Park, Soungsill;Yoon, Hyoseok;Park, Seho
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.13 no.5
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    • pp.2509-2528
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    • 2019
  • There are various styles of license plates for different countries and use cases that require style-specific methods. In this paper, we propose and illustrate a multi-style license plate recognition system. The proposed system performs a series of processes for license plate candidates detection, structure classification, character segmentation and character recognition, respectively. Specifically, we introduce a license plate structure classification process to identify its style that precedes character segmentation and recognition processes. We use a K-Nearest Neighbors algorithm with pre-training steps to recognize numbers and characters on multi-style license plates. To show feasibility of our multi-style license plate recognition system, we evaluate our system for multi-style license plates covering single line, double line, different backgrounds and character colors on Korean and the U.S. license plates. For the evaluation of Korean license plate recognition, we used a 50 minutes long input video that contains 138 vehicles of 6 different license plate styles, where each frame of the video is processed through a series of license plate recognition processes. From two experiments results, we show that various LP styles can be recognized under 50 ms processing time and with over 99% accuracy, and can be extended through additional learning and training steps.

Performance Improvement of Cardiac Disorder Classification Based on Automatic Segmentation and Extreme Learning Machine (자동 분할과 ELM을 이용한 심장질환 분류 성능 개선)

  • Kwak, Chul;Kwon, Oh-Wook
    • The Journal of the Acoustical Society of Korea
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    • v.28 no.1
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    • pp.32-43
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    • 2009
  • In this paper, we improve the performance of cardiac disorder classification by continuous heart sound signals using automatic segmentation and extreme learning machine (ELM). The accuracy of the conventional cardiac disorder classification systems degrades because murmurs and click sounds contained in the abnormal heart sound signals cause incorrect or missing starting points of the first (S1) and the second heart pulses (S2) in the automatic segmentation stage, In order to reduce the performance degradation due to segmentation errors, we find the positions of the S1 and S2 pulses, modify them using the time difference of S1 or S2, and extract a single period of heart sound signals. We then obtain a feature vector consisting of the mel-scaled filter bank energy coefficients and the envelope of uniform-sized sub-segments from the single-period heart sound signals. To classify the heart disorders, we use ELM with a single hidden layer. In cardiac disorder classification experiments with 9 cardiac disorder categories, the proposed method shows the classification accuracy of 81.6% and achieves the highest classification accuracy among ELM, multi-layer perceptron (MLP), support vector machine (SVM), and hidden Markov model (HMM).

New Isolated Zero Voltage Switching PWM Boost Converter (새로운 절연된 영전압 스위칭 PWM 부스트 컨버터)

  • Cho, Eun-Jin;Moon, Gun-Woo;Jung, Young-Suk;Youn, Myung-Joong
    • Proceedings of the KIEE Conference
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    • 1994.07a
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    • pp.535-538
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    • 1994
  • In this paper, an isolated ZVS-PWM boost converter is proposed for single stage line conversion. For power factor correction, we used the half bridge topology at the primary side of isolation transformer permitting switching devices to operate under ZVS by using circuit parastics and operating at a fixed duty ratio near 50%. Thus the relatively continuous input current distortion and small size input filter are also achievable. The ZVS-PWM boost operation of the proposed converter can be achieved by using the boost inductor $L_f$, main switch $Q_3$, and simple auxiliary circuit at the secondary side of isolation transformer. The secondary side circuit differ from a conventional PWM boost converter by introduction a simple auxiliary circuit. The auxiliary circuit is actived only during a short switching transition time to create the ZVS condition for the main switch as that of the ZVT-PWM boost converter. With a single stage, it is possible to achieve a sinusoidal line current at unity power factor as well as the isolated 48V DC output. Comparing to the two stage schemes, overall effiency of the proposed converter is highly improved due to the effective ZVS of all devices as well as single stage power conversion. Thus, it can be operated at high switching frequency allowing use of small size input filter. Minimum voltage and current stress make it high power application possible.

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General prescription pattern of insured herbal preparation in South Korea: A nationwide cohort study (건강보험용 한약제제의 처방 패턴에 대한 기술역학적 분석: 건강보험공단 표본코호트 2.0 분석 (2010-2019))

  • Heekyung Kim;Mangyeong Lee;Jaegon Kim;Juhee Cho
    • The Journal of Korean Medicine
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    • v.45 no.3
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    • pp.14-30
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    • 2024
  • Objectives: Research on the general prescription patterns of insured herbal preparations in Korea has been limited. This study aimed to analyze prescription patterns of insured herbal preparations, utilizing the National Health Insurance Service (NHIS) sample cohort 2.0 data from 2010 to 2019. Methods: NHIS sample cohort 2.0 database, which represents a sample of 2.2% of the Korean population, was analyzed. We analyzed data related to prescriptions recognized as insured herbal preparations. Variables included patient demographics, diagnosis codes, prescription details, and healthcare institution characteristics. We examined trends over the decade, focusing on herbal formulae and single herb extracts. Results: During the study period, 275,358 patients visited Korean traditional medicine clinics and received at least one prescription of herbal preparations, representing 27.5% of the total sample. The number of prescriptions increased by 209%, from 34,621 in 2010 to 72,553 in 2019. Females accounted for 67% of these prescriptions, and 77% were for patients aged 70 and older. The top ten herbal formulae (TTHF), including Ojeok-san and Gungha-tang, constituted 76% of all prescriptions. Herbal formulae were used more frequently than single herb extracts, with certain prescriptions being commonly utilized. There was a notable focus on musculoskeletal disorders, with low back pain being the most common diagnosis. Conclusion: The study demonstrates a significant increase in the use of insured herbal preparations, primarily in small clinics and among the elderly. Prescription patterns showed a preference for specific single herb extracts and herbal formulae, with consistent trends over time. These findings provide valuable insights for future clinical research and policy development, particularly as herbal medicine's role in the national healthcare system continues to expand.

An User Authentication Mechanism in the OSGi Service Platform Environment (OSGi서비스 플랫폼 환경에서의 사용자 인증 메커니즘)

  • 전경석;문창주;박대하;백두권
    • Journal of KIISE:Computing Practices and Letters
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    • v.9 no.2
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    • pp.191-204
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    • 2003
  • In the home gateway environment, several characteristics for the user authentication mechanism should be reflected separately from the existing distributed service environment. First, the platform of a home gateway is a component based system that its services are installed dynamically. Second, the convenience that user can use several services by authentication of once should be offered. Finally, the system resources of a home gateway are restricted. However, a user authentication mechanism that reflected these characteristics is not shown at the user admin service specification of the OSGi service platform.(OSGi is the representative standardization organization of hone gateway.) Also, there is no existing authentication protocol that satisfies these qualities at the same time. In this paper, we propose a new user authentication mechanism considering those characteristics for the home gateway environment. We also design and implement an independent authentication service bundle based on the OSGi service platform so that it can perform user authentication operations for each bundle service. We supplement and extend the Kerberos Protocol that can apply.