• Journal of Korean Society of Environmental Engineers
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• v.32 no.4
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• pp.341-348
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• 2010

The basic principle of fry drying process of sludge lies in the rapid pressure change of sludge material caused by the change of temperature between oil and moisture due to the difference of specific heat. Therefore, the rapid increase of pressure in drying sludge induces the efficient moisture escape through sludge pores toward heating oil media. The object of this study is to carry out a systematic investigation of the influence of various parameters associated with the sludge fry drying processes on the drying efficiency. To this end, a series of parametric experimental investigation has been made together with the numerical calculation in order to obtain typical drying curves as function of important parameters such as drying temperature, sludge diameter, oil type and sludge type. In the aspect of frying temperature, especially it is found that the operation higher than $140^{\circ}C$ was favorable in drying efficiency regardless of type of waste oil employed in this study. The same result was also noted consistently in the investigation of numerical calculation, that is, in that the sludge particle drying was efficiently made over $140^{\circ}C$ irrespective of the change of particle diameter. As expected, in general, the decrease of diameter in sludge was found efficient both experiment and numerical calculation in drying due to the increased surface area per unit volume. In the investigation of oil type and property, the effect of the viscosity of waste oil was found to be more influential in drying performance. In particular, when the oil with high viscosity, a visible time delay was noticed in moisture evaporation especially in the early stage of drying. However, the effect of high viscosity decreased significantly over the temperature of $140^{\circ}C$. There was no visible difference observed in the study of sludge type but the sewage sludge with a slightly better efficiency. The numerical study is considered to be a quite useful tool to assist in experiment with more detailed empirical modeling as further work.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.14
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• pp.6073-6080
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• 2015

Background: Melanoma differentiation-associated gene-7 (MDA-7)/interleukin-24 (IL-24), a unique tumor suppressor gene, has killing activity in a broad spectrum of cancer cells. Herein, plasmids producing mda-7 proteins fused to different RGD peptides (full RGD4C and shortened RGD, tRGD) were evaluated for apoptosis induction with a hepatocellular carcinoma cell line, Hep-G2. The study aim was to improve the apoptosis potency of mda-7 by tethering to RGD peptides. Materials and Methods: Three plasmids including mda-7, mda-7-RGD and mda-7-tRGD genes beside a control vector were transfected into Hep-G2 cells. After 72 hours incubation, cell viability was evaluated by MTT assay. In addition, the rate of apoptosis was analyzed by flow cytometry using PI/annexin staining. To detect early events in apoptosis, 18 hours after transfection, expression of the BAX gene was quantified by real time PCR. Modeling of proteins was also performed to extrapolate possible consequences of RGD modification on their structures and subsequent attachment to receptors. Results and Conclusions: In MTT assays, while all mda-7 forms showed measurable inhibition of proliferation, unmodified mda-7 protein exhibited most significant effect compared to control plasmid (P<0.001). Again, flow cytometry analysis showed a significant apoptosis induction by simple mda-7 gene but not for those RGD-fused mda-7 proteins. These findings were also supported by expression analysis of BAX gene (P<0.001). Protein modelling analysis revealed that tethering RGD at the end of IL-24/Mda7 disrupt attachment to cognate receptor, IL-20R1/IL-20R2. In conclusion, fusion of RGD4C and shortened RGD peptides to carboxyl terminal of mda7, not only reduce apoptosis property in vitro but also disrupt receptor attachment as demonstrated by protein modelling.

• Journal of the Korean Chemical Society
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• v.52 no.1
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• pp.57-65
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• 2008

are nanometer or micrometer scale vesicles that can be used as drug delivery carriers. However, plain liposomes are plagued by rapid opsonization, making their circulation time in bloodstream be shortened. In this study, model protein, bovine serum albumin (BSA)-coated liposomes were prepared by coating cationic liposomes with BSA molecules at higher pH than isoelectric point of BSA. The BSA molecules coated on the liposomal surface were denatured by thermal treatment at above 60oC. While both plain and cationic liposomes had about mean particle diameter of 1041 nm, BSA-coated cationic liposomes (BCL) had mean particle diameter of 1091 nm. Encapsulation of model drug, doxorubicin (DOX), in liposomes were carried out by using remote loading method and the loading efficiency of DOX to liposomes was about 90%. The mean particle diameter of BCL did not increase in blood plasma and adsorption of plasma protein was much less than plain or cationic liposomes. These results suggest that BCL can be used as a long-circulating liposomes in bloodstream.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.4
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• pp.309-314
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• 2002

Rhtological properties of surimi gel from white fishes by acid (acid surimi) and alkali (alkali surmi) process and effect of chemicals on gelation were investigated by punch and dynamic tests. The breaking force and deformation values of heat-induced gel of acid surimi were less than their values of alkali and conventional surimi gel, and whiteness was greatly decreased, Gel point of acid surimi was decreased but it of alkali surimi was increased with increasing moisture content in the range of 80 to $85\%$. Storage modulus of acid surimi was the highest vaule in pH 6.8, but that of alkali surimi showed high value at neutral and slightly alkali pH. Propylene glycol increased storage modulus in $20\~50^{\circ}C$, hut urea and 2-mercaptoethanol suppressed it. Potassium bromide improved storage modulus in $20~80^{\circ}C$, The results suggest that alkai process is used for making surimi instead of conventional method.

• Journal of the Korean Applied Science and Technology
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• v.31 no.3
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• pp.500-508
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• 2014

For this research, synthesis acrylic resin by ethyl acrylate monomer(EAM) and prepared samples which set by difference amount of monoammonium phosphate solution in waterborne acrylic resin. Use these resins, analyzed mechanical properties and thermal stability by films and leather surface coated. The test of DSC experiment sample WAC-APS3 was $410^{\circ}C$ Tm values which means the highest content of monoammonium phosphate had highest thermal stability in acrylic resin. According to measure data for solvent resistance, all samples showed good property. As known in the results, increase of ammonium phosphate constant did not influence to big change of resin properties. In abrasion test WAC-APS3 was good abrasion properties(68.729 mg.loss). Test of tensile strength, as increase as monoammonium phosphate resin analyzed low properties $1.505kgf/mm^2$ to $1.275kgf/mm^2$. In elongation case, same as strength test result 425 % to 384 % by increase to monoammonium phosphate amount in acrylic resin.

• Korean Journal of Food Science and Technology
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• v.42 no.5
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• pp.613-619
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• 2010

Three varieties of taro flours (Altoran, Josaengjong, and Jaeraejong) were analyzed to evaluate their nutrient composition and physicochemical properties. Moisture contents of the three taro cultivars varied from 5.74-10.30%. Among the three cultivars, Altoran flour had higher protein and fat contents than other cultivars. There were 17 kinds of amino acids in the three taro flours. The major fatty acids in the three taro flours were linoleic acid (46.5-51.4%), palmitic acid (21.7-25.8%), and oleic acid (12.3-18.7%), and two thirds of the total fatty acids were unsaturated fatty acids. Potassium, phosphorus, magnesium, and calcium were the most plentiful minerals in the three taro flours, and the free sugars were glucose, fructose, and sucrose. The total dietary fiber contents of the three taro flours ranged from 12.97 to 17.60%. From these results, Altoran contained a high level of dietary fiber, minerals, essential amino acids, and free sugars. Jaeraejong showed the highest water absorption index. The water soluble index of Altoran was higher than that of the other cultivars. From the differential scanning calorimeter results, the gelatinization temperatures of the three taro flours were $84.98-89.91^{\circ}C$. The pasting temperature of the three taro flours was from 46.77 to $49.30^{\circ}C$ by rapid visco-analyzer. Among these varieties, Josaengjong had the highest peak viscosity and final viscosity, whereas Altoran had the lowest. These taro cultivars are expected to be potential material for application in taro processed foods, due to their gelatination and pasting properties.

• Journal of Korean Society of Forest Science
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• v.86 no.3
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• pp.311-318
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• 1997

Chemical properties of soil(N, P, K, Ca, Na, Mg, CEC, and pH) were studied after annual additions of $NH_4NO_3$(336kg/ha N), treble superphosphate(112kg/ha P), and KCl(224kg/ha K) fertilizers in a willow(Salix spp.) bioenergy plantation. Soil samples were collected from November through December 1992 from previously established the fertilized and non-fertilized willow plantation at Tully, New York, U.S.A. in 1987. Total fertilizer additions from 1987 through 1991 were 1,680kg/ha N and 560kg/ha P and 1.120kg/ha K. Fertilization with N, P, and K resulted in no difference in total soil N content between the fertilized and non-fertilized plots, increased soil P and K, decreased base cations ($Ca^{2+}$ and $Mg^{2+}$) and soil pH, and increased soil pH with soil depth. Strong positive correlations of soil carbon to soil N, Ca, Mg, and CEC were noted. Soil C/N ratio in the study plots ranged from 9.6 to 11.2 for all treatment combinations. Significant differences in soil P, K, Ca, and pH between the fertilized and non-fertilized plots indicate that fertilization had changed chemical properties of soil in this fertilizer trial.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.46 no.7
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• pp.7-14
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• 2009

In this paper, the effects of annealing conditions on the structural ((002) intensity, FWHM, d-spacing, grain size, (002) peak position), optical (UV peak, UV peak position) and electrical properties (carrier concentrations, resistivity, mobility) of ZnO films were investigated. ZnO films were deposited onto SiO$_2$/si substrates by RF magnetron sputtering from a ZnO target. The substrate was not heated during deposition. ZnO films were annealed in temperature ranges of $500\sim650^{\circ}C$ in the O$_2$ flow for 5$\sim$20 min. The film average thicknesses were in the range of 291 nm. The surface morphologies and structures of the samples were characterize by SEM and XRD, respectively. The optical properties were evaluated by photoluminescence (PL) measurement at room temperature (RT) using a He-Cd 325 nm laser. As the annealing temperature and time vary, the following relations were also observed: (1) proportional relationships among UV intensity (002) intensity, and grain size exist, (2) UV intensity is inversely proportional to FWHM, (3) there is no special relationship between UV intensity and electron carrier concentrations, (4) d-spacing is inversely proportional to (002) peak position, (5) UV peak position in the range of 3.20$\sim$3.24 eV means that ZnO films have a n-type conductivity which was consistent with that obtained from the electrical property, (6) the optimal conditions for the best optical and structural characteristics were found to be oxygen fraction, (O$_2$/(O$_2$+Ar)) of 0.2, RF power of 240W, substrate temperature of RT, annealing condition of 600$^{\circ}C$ for 20 min, and sputtering pressure of 20 mTorr.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2012.03a
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• pp.89-89
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• 2012

선행 연구에서는 m-Aramid와 p-Aramid의 Ring 방적사/Core 방적사 조건에 따른 방적사 물성에 대해서 연구하였는데, Ring 방적사의 혼용율, 스핀들 속도에 따른 물성 변화, Core 방적사의 공정특성에 따른 방적성, 피복성에 대해 연구를 하였다. 그 결과 Ring 방적사의 경우 m-Aramid의 혼용율이 증가할수록 사 강도는 증가하는 경향을 확인할 수 있었고, 스핀들 속도가 증가함에 따라서 불균제도 및 사결점이 다소 증가하였다. Core 방적사의 경우 Core에 p-Aramid를 사용했을 때 방적성과 피복성은 양호 하였지만, 강한 p-Aramid에 의해 톱 롤러코트의 마모가 되는 경향을 볼 수 있었다. 본 연구에서는 Ring 방적사/Core 방적사에 대해 좀더 구체적인 제조공정조건에 의한 물성 변화를 알아보았고, 좀더 다양한 섬유소재를 적용해서 제조하였다. Ring 방적사의 경우 p-Aramid 혼용율에 따른 실험을 하였는데, m-Aramid에 대한 p-Aramid의 혼용율을 0%, 5%, 10%, 20% 로 하여 Ne30을 제조하였으며, Core 방적사는 Core를 p-Aramid 200D로 하고 Sheath를 Cotton으로 하여 Core 공급속도비 (1.06, 1.10, 1.14), 연계수(T/M, 3.8, 4.0, 4.2), Sheath/Core 혼섬율(70/30, 60/40, 50/50)에 따라 제조하였다. 추가로 Core 방적사는 Sheath에 Cotton 대신 FR-Rayon과 선염 Cotton을 사용하여 각각 Sheath/Core 혼섬율 70/30의 비율로 방적사를 제조하여 다양한 소재에 따른 방적사의 물성을 측정하였다. 제조된 시험 원사들의 측정 물성은 번수(Ne), 균제도(U%), 사결점(IPI), 강력(cN), 신도(%), 비강도(cN/Tex) 등이며, 편직을 통해 편성물의 외관을 확인하였다. Ring 방적사의 경우 p-Aramid의 혼용율이 증가할수록 강도는 완만하게 증가한 반면, 신도는 급격하게 감소하였다. Cotton Core 방적사의 경우는 공급속도비가 높아질수록 균제도가 높아지는 것을 볼수 있었고, Core 공급속도비가 높아질수록 외관상 피복도는 높아진 것을 볼 수 있었다. 이렇게 연구된 Aramid 소재를 이용한 방적사 제조 공정조건변화에 따른 물성 및 피복도 결과는 기존 방적업체에서 Aramid를 이용한 방적사 제조시에 공정조건을 확보하는데 도움이 될 것이라 생각되며, 방적사 시제품 생산시에 발생할 수 있는 시행착오를 줄임으로써 시제품 제조를 위한 생산비용의 Loss를 절감할 수 있을 것으로 예상된다.

• Journal of Life Science
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• v.25 no.6
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• pp.631-637
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• 2015

Streptavidin, a protein produced by Streptomyces avidinii, strongly binds up to four molecules of vitamin H, d-biotin exhibiting the dissociation constant of about 10⁻¹⁵ M. This strong binding affinity has been applied for detection and characterization of numerous biological molecules suggesting expression and purification of functional streptavidin should be very useful for the application of this streptavidin-biotin interaction. To express a soluble streptavidin in Escherichia coli, We synthesized streptavidin genes and cloned into pET-22b plasmid, which uses T7 RNA polymerase/T7 promoter expression systems containing pelB leader for secretion into periplasmic space and six polyhistidine tags at C-terminus for purification of expressed proteins. Although streptavidin is toxic to Escherichia coli due to strong biotin binding property, streptavidin was expressed very sufficiently in a range of 10-20 mg/ml. In SDS-PAGE, the size of purified protein was shown as 17 kDa in denatured condition (boiling) and 68 kDa in native condition (without boiling) suggesting tetramerization of monomeric subunit by non-covalent association. Further analysis by size-exclusion chromatography supported streptavidin’s tetrameric structure as well. In addition, soluble streptavidin detected biotinylated proteins in westernblot indicating its functional activity to biotin. Taken these results together, it concluded that our simple expression system was able to show high yield, homotetrameric formation and biotin binding activity analogous to natural streptavidin.