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Effects of Long-term Exposure to Black Carbon Particles on Growth and Gas Exchange Rates of Fagus crenata, Castanopsis sieboldii, Larix kaempferi and Cryptomeria japonica Seedlings

  • Yamaguchi, Masahiro;Otani, Yoko;Takeda, Kenta;Lenggoro, I. Wuled;Ishida, Atsushi;Yazaki, Kenichi;Noguchi, Kyotaro;Sase, Hiroyuki;Murao, Naoto;Nakaba, Satoshi;Yamane, Kenichi;Kuroda, Katsushi;Sano, Yuzou;Funada, Ryo;Izuta, Takeshi
    • Asian Journal of Atmospheric Environment
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    • v.6 no.4
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    • pp.259-267
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    • 2012
  • To clarify the effects of black carbon (BC) particles on growth and gas exchange rates of Asian forest tree species, the seedlings of Fagus crenata, Castanopsis sieboldii, Larix kaempferi and Cryptomeria japonica were exposed to BC particles with sub-micron size for two growing seasons from 1 June 2009 to 11 November 2010. The BC particles deposited after the exposure to BC were observed on the foliar surface of the 4 tree species. At the end of the experiment, the amount of BC accumulated on the foliar surface after the exposure to BC aerosols were 0.13, 0.69, 0.32 and 0.58 mg C $m^{-2}$ total leaf area in F. crenata, C. sieboldii, L. kaempferi and C. japonica seedlings, respectively. In August 2010, the exposure to BC particles did not significantly affect net photosynthetic rate under any light intensity, stomatal diffusive conductance to water vapour ($g_s$), stomatal limitation of photosynthesis, response of $g_s$ to increase in vapour pressure deficit and leaf temperature under light saturated condition in the leaves or needles of the seedlings. These results suggest that the BC particles deposited on the foliar surface did not reduce net photosynthesis by shading, did not increase leaf temperature by absorption of irradiation light, and did not induce plugging of stomata in the leaves or needles of the seedlings. There were no significant effects of BC particles on the increments of plant height and stem base diameter during the experimental period and the whole-plant dry mass at the end of the experiment. These results indicate that the exposure to BC particles with sub-micron size for two growing seasons did not significantly affect the growth and leaf or needle gas exchange rates of F. crenata, C. sieboldii, L. kaempferi and C. japonica seedlings.

PQQ-Dependent Organic Acid Production and Effect on Common Bean Growth by Rhizobium tropici CIAT 899

  • Cho, Young-Shin;Park, Ro-Dong;Kim, Yong-Woong;Hwangbo, Hoon;Jung, Woo-Jin;Suh, Jang-Sun;Koo, Bon-Sung;Krishnan, Hari-B.;Kim, Kil-Yong
    • Journal of Microbiology and Biotechnology
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    • v.13 no.6
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    • pp.955-959
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    • 2003
  • Rhizobium tropici CIAT 899 is capable of synthesizing inactive apo-glucose dehydrogenase (GDH). To become an active holo enzyme, the GDH requires a cofactor, PQQ. When R. tropici CIAT 899 was grown in a broth culture medium containing hydroxyapatite and pyrrolo quinoline quinone (PQQ), pH decreased while the concentration of soluble P increased. The solubilization of hydroxyapatite was associated with the production of gluconic acid and 2-ketogluconic acids. The organic acid production and P solubilization were greatly enhanced when the bacterium was grown with air supply. Effect of R. tropici CIAT 899 with (CI+PQQ) and without PQQ (CI) on the common bean growth was examined. Shoot and root weight, and N and P contents in CI+PQQ treatment, were significantly higher than those in control and CI treatment. Nodule weight and acetylene reducing activities were also significantly higher in CI+PQQ treatment than in other treatments.

Isolation, Screening and Identification of Swine Gut Microbiota with Ochratoxin A Biodegradation Ability

  • Upadhaya, Santi Devi;Song, Jae-Yong;Park, Min-Ah;Seo, Ja-Kyeom;Yang, Liu;Lee, Chan-Ho;Cho, Kyung-J.;Ha, Jong-K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.1
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    • pp.114-121
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    • 2012
  • The potential for ochratoxin A (OTA) degradation by swine intestinal microbiota was assessed in the current study. Intestinal content that was collected aseptically from swine was spiked with 100 ppb OTA and incubated for 6 and 12 h at $39^{\circ}C$. An OTA assay was conducted using the incubated samples, and it was found that 20% of the OTA toxin was detoxified, indicating the presence of microbes capable of OTA degradation. Twenty-eight bacterial species were isolated anaerobically in M 98-5 media and 45 bacterial species were isolated using nutrient broth aerobically. Screening results showed that one anaerobic bacterial isolate, named MM11, detoxified more than 75% of OTA in liquid media. Furthermore, 1.0 ppm OTA was degraded completely after 24 h incubation on a solid 'corn' substrate. The bacterium was identified by 16S rDNA sequencing as having 97% sequence similarity with Eubacterium biforme. The isolation of an OTA-degrading bacterium from the swine natural flora is of great importance for OTA biodegradation and may be a valuable potential source for OTA-degradation enzymes in industrial applications.

Development of CFD model for Predicting Ventilation Rate based on Age of Air Theory using Thermal Distribution Data in Pig House (돈사 내부 열환경 분포의 공기연령 이론법 적용을 통한 전산유체역학 환기 예측 모델 개발)

  • Kim, Rack-woo;Lee, In-bok;Ha, Tae-hwan;Yeo, Uk-hyeon;Lee, Sang-yeon;Lee, Min-hyung;Park, Gwan-yong;Kim, Jun-gyu
    • Journal of The Korean Society of Agricultural Engineers
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    • v.59 no.6
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    • pp.61-71
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    • 2017
  • The tracer gas method has an advantage that can estimate total and local ventilation rate by tracing air flow. However, the field measurement using tracer gas has disadvantages such as danger, inefficiency, and high cost. Therefore, the aim of this study was to evaluate ventilation rate in pig house by using the thermal distribution data rather than tracer gas. Especially, LMA (Local Mean Age), which is an index based on the age of air theory, was used to evaluate the ventilation rate in pig house. Firstly, the field experiment was conducted to measure micro-climate inside pig house, such as the air temperature, $CO_2$ concentration and wind velocity. And then, LMA was calculated based on the decay of $CO_2$ concentration and air temperature, respectively. This study compared between LMA determined by $CO_2$ concentration and air temperature; the average error and root mean square error were 3.76 s and 5.34 s. From these results, it was determined that thermal distribution data could be used for estimation of LMA. Finally, CFD (Computational fluid dynamic) model was validated using LMA and wind velocity. The mesh size was designed to be 0.1 m based on the grid independence test, and the Standard $k-{\omega}$ model was eventually chosen as the proper turbulence model. The developed CFD model was highly appropriate for evaluating the ventilation rate in pig house.

Internal Thermal Environment Uniformity Analysis of Mechanically Ventilated Broiler House (강제 환기식 육계사 내부 열환경 균일성 평가)

  • Kim, Da-in;Lee, In-bok;Lee, Sang-yeon;Park, Sejun;Kim, Jun-gyu;Cho, Jeong-hwa;Jeong, Hyo-hyeog;Kang, Sol-moe;Jeong, Deuk-young
    • Journal of The Korean Society of Agricultural Engineers
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    • v.64 no.6
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    • pp.65-75
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    • 2022
  • Livestock industry in Korea has been growing rapidly and has reached 23 trillion Korean won in 2021. This study focuses on broiler, which is one of the largest sectors in livestock industry. As the effects of climate change get more serious, primary industry such as livestock industry is fragile to climate change since it directly interacts with nature. Therefore, maintaining suitable rearing environment is important. One of the most frequently used ventilation type for controlling the rearing environment of broiler house, tunnel ventilation, causes frequent air velocity fluctuation which makes maintaining the rearing environment important. By measuring the air temperature, relative humidity and air velocity in various points inside the broiler house, the internal thermal environment uniformity was analyzed according to length, width and zone. The experimental house was found to have dead zone with high air temperature, relative humidity and low air velocity near the end of the inlet and at the end of the broiler house. By using heat stress index to analyze quantitatively, zone with highest heat stress index was found to increase by 7.55% compared to the lowest zone. As a result, to maintain uniform rearing environment inside the broiler house, different factors must be measured and analyzed and used to operate the environmental control facilities.

Comprehensive comparative analysis of chloroplast genomes from seven Panax species and development of an authentication system based on species-unique single nucleotide polymorphism markers

  • Nguyen, Van Binh;Giang, Vo Ngoc Linh;Waminal, Nomar Espinosa;Park, Hyun-Seung;Kim, Nam-Hoon;Jang, Woojong;Lee, Junki;Yang, Tae-Jin
    • Journal of Ginseng Research
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    • v.44 no.1
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    • pp.135-144
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    • 2020
  • Background: Panax species are important herbal medicinal plants in the Araliaceae family. Recently, we reported the complete chloroplast genomes and 45S nuclear ribosomal DNA sequences from seven Panax species, two (P. quinquefolius and P. trifolius) from North America and five (P. ginseng, P. notoginseng, P. japonicus, P. vietnamensis, and P. stipuleanatus) from Asia. Methods: We conducted phylogenetic analysis of these chloroplast sequences with 12 other Araliaceae species and comprehensive comparative analysis among the seven Panax whole chloroplast genomes. Results: We identified 1,128 single nucleotide polymorphisms (SNP) in coding gene sequences, distributed among 72 of the 79 protein-coding genes in the chloroplast genomes of the seven Panax species. The other seven genes (including psaJ, psbN, rpl23, psbF, psbL, rps18, and rps7) were identical among the Panax species. We also discovered that 12 large chloroplast genome fragments were transferred into the mitochondrial genome based on sharing of more than 90% sequence similarity. The total size of transferred fragments was 60,331 bp, corresponding to approximately 38.6% of chloroplast genome. We developed 18 SNP markers from the chloroplast genic coding sequence regions that were not similar to regions in the mitochondrial genome. These markers included two or three species-specific markers for each species and can be used to authenticate all the seven Panax species from the others. Conclusion: The comparative analysis of chloroplast genomes from seven Panax species elucidated their genetic diversity and evolutionary relationships, and 18 species-specific markers were able to discriminate among these species, thereby furthering efforts to protect the ginseng industry from economically motivated adulteration.

A Plan to Establish Agriculture and Fisheries Special Channel - Marketing Expert, Farmer and Fisher, Broadcasting Expert FGI Result - (농수산식품 전문채널 설립방안 - 농수산 마케팅 전문가, 농수산업인, 방송전문가 FGI 결과 -)

  • Park, Ji Seob;Moon, Junghoon;Kim, Jin Gi
    • Agribusiness and Information Management
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    • v.2 no.1
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    • pp.15-29
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    • 2010
  • Recently, IPTV population is growing by leaps and bounds. and We performed FGI(Focus Group Interview) with various experts related to agriculture field to establish Agriculture and Fisheries special channel. this growth has social background that is change of paradigm caused by the convergence of broadcasting, demand for Agriculture and Fisheries special channel using new media thanks to interests in Wellbeing, Green food. so we think that we need to consider the way to establish Agriculture and Fisheries special channel and its validity from all angles. that is why we are having a heated discussion with Agriculture and Fisheries marketing experts, farmer, fisher(Korean Cyber Farmer Association), broadcasting experts. Concretely, government budget is needed to establish Agriculture and Fisheries special channel, so we are just about to examine its validity and find business model. to make this discussion detailed, we made 4 subjects. First, Do we need agro-fishery products distribution channel using media? Second, After establishing of Agriculture and Fisheries special channel, how many demands will occur? Third, Should we make Agriculture and Fisheries special channel public channel? Fourth, What would be profit model of Agriculture and Fisheries special channel?

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In vitro Antioxidant Properties and Phenolic Composition of Korean Commercial Vinegars

  • Jeong, Chang-Ho;Choi, Gwi-Nam;Kim, Ji-Hye;Kwak, Ji-Hyun;Kang, Su-Tae;Choi, Sung-Gil;Heo, Ho-Jin
    • Food Science and Biotechnology
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    • v.18 no.5
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    • pp.1258-1262
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    • 2009
  • Total phenolics and antioxidant properties of various Korean commercial vinegars (apple vinegar, AV; blueberry vinegar, BV; grape vinegar, GV; lemon vinegar, LV; Opuntia ficus vinegar, OFV; persimmon vinegar, PV; Prunus mume vinegar, PMV; rice vinegar, RV) were investigated. The total phenolic contents of 8 vinegars were within the range of 54.18-491.02${\mu}g/mL$. The vinegars were also capable of scavenging 1,1-dipehnyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radicals in a manner dependent on concentration. The greatest reducing power was observed in PV relative to the other vinegars. The ferric reducing ability of plasma (FRAP) of PV, PMV, GV, and BV were 1.012, 0.969, 0.931, and 0.856 at a dose of 1 mL, respectively. Therefore, our study verified that the GV, PV, and PMV have powerful antioxidant activities which are correlated with its high level of phenolics, particularly gallic acid, and epigallocatechin.

A study to improve the national technical qualification practical evaluation method of National Competency Standards in the field of organic agriculture

  • Hyun-Ho, Jang;Taek-Keun, Oh;Jwakyung, Sung
    • Korean Journal of Agricultural Science
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    • v.48 no.4
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    • pp.855-863
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    • 2021
  • The purpose of this study is to suggest an improvement plan for the National Technical Qualification Practical Evaluation focusing on National Competency Standards. First, in order to strengthen practical techniques such as field abilities and expertise, practical evaluation methods were applied to the National Competency Standards, referring to a learning module focused on field performance. Second, an expert advisory committee in the field of organic agriculture was utilized to compare, analyze, and match the competency unit and competency unit elements of the National Competency Standards with the national technical qualification examination standards in field of organic agriculture. In addition, in order to identify work in progress in the industrial field, share professional practice skills, and analyze facilities and equipment, we visited an organic agriculture industry site. Through this, a practical evaluation exam was developed. Subsequently, in order to confirm the adequacy of the developed exam, the pilot test assessment was conducted for those majoring in organic agriculture. Finally, as a feasibility study, a survey was conducted. Based on the results, greater technical job competency in the field of organic agriculture will contribute to strengthening knowledge in this field by suggesting an improvement plan for the national technical qualifications practical evaluation method in the field of organic agriculture.

Administration of chromium picolinate and meloxicam alleviates regrouping stress in dairy heifers

  • Da Jin Sol Jung;Jaesung Lee;Do Hyun Kim;Seok-Hyeon Beak;Soo Jong Hong;In Hyuk Jeong;Seon Pil Yoo;Jin Oh Lee;In Gu Cho;Dilla Mareistia Fassah;Hyun Jin Kim;Mohammad Malekkhahi;Myunggi Baik
    • Animal Bioscience
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    • v.37 no.8
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    • pp.1495-1502
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    • 2024
  • Objective: This research investigated the effect of administering chromium (Cr) and meloxicam (MEL) on growth performance, cortisol and blood metabolite, and behaviors in young, regrouped heifers. Methods: Fifty Holstein dairy heifers (body weight [BW] 198±32.7 kg and 6.5±0.82 months of age) were randomly assigned to non-regrouped group or four regrouped groups. Non-regrouped animals were held in the same pen throughout the entire experimental period (NL: non-regrouping and administration of lactose monohydrate [LM; placebo]). For regrouping groups, two or three heifers maintained in four different pens for 2 weeks were regrouped into a new pen and assigned to one of four groups: regrouping and LM administration (RL); regrouping and Cr administration (RC); regrouping and MEL administration (RM), and regrouping and Cr and MEL administration (RCM). LM (1 mg/kg BW), Cr (0.5 mg Cr picolinate/kg dry matter intake), and MEL (1 mg/kg BW) were orally administered immediately before regrouping. Blood was collected before regrouping (0 h) and at 3, 9, and 24 h and 7 and 14 d thereafter. Behaviors were recorded for 7 consecutive days after regrouping. Results: Average daily gain was lower (p<0.05) in RL than NL heifers, but was higher (p<0.05) in RM, RC, and RCM than RL heifers. RL heifers had higher (p<0.05) cortisol than NL heifers on d 1 after regrouping. The cortisol concentrations in RC, RM, and RCM groups were lower (p<0.05) than in RL treatment 1 d after regrouping. Displacement behavior was greater (p<0.05) in RL group than all other groups at 2, 3, and 6 d after regrouping. Conclusion: Regrouping caused temporal stress, reduced growth performance, and increased displacement behavior in heifers. Administering Cr and MEL recovered the retarded growth rate and reduced displacement behavior, thereby alleviating regrouping stress.