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Effect of Blanching Conditions and Thawing Methods on Quality Properties of Platycodon grandiflorum (데침 조건과 해동 방법이 도라지의 품질 특성에 미치는 영향)

  • Choi, Soo Young;Lee, Sang Yoon;Davaatseren, Munkhtugs;Yoo, Seon Mi;Choi, Mi Jung;Han, Hye Min
    • Culinary science and hospitality research
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    • v.20 no.6
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    • pp.211-222
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    • 2014
  • This study was performed to identify the quality characteristic of Platycodon grandiflorum for blanching(1, 2, 3 min), drying(5, 10, 15 min) conditions and thawing methods($4^{\circ}C$, $25^{\circ}C$, running water). The color, moisture contents, pH, hardness, viable cell count of blanched Platycodon grandiflorum were lower than those of native Platycodon grandiflorum. The sensory properties of blanched Platycodon grandiflorum for blanching and drying time showed the most highly evaluated in terms of texture. The optimum blanching and drying times selected for producing of frozen Platycodon grandiflorum were 1 min, 5 min. At this condition, the blanched Platycodon grandiflorum was frozen at $-40^{\circ}C$. After thawing of frozen Platycodon grandiflorum, the thawing time was the shortest in the case of thawing in running water, and drip loss was the lowest. The color, hardness and sensory properties did not significantly differ with different thawing methods. These results show that thawing in running water is the most suitable for frozen Platycodon grandiflorum.

A Subcutaneous Lipoma in a Male Red Fox (여우에서 피하지방종의 진단)

  • Jeong, Dong-hyuk;Yang, Jeong-jin;Kong, Joo-yeon;Lee, Bae-keun;Lee, Je-wook;Park, Se-jin;Lee, Seung-yong;Seok, Seong-hoon;Hong, Il-hwa;Lee, Hee-chun;Yeon, Seong-chan
    • Journal of Veterinary Clinics
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    • v.32 no.3
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    • pp.278-281
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    • 2015
  • An 8-year-old male red fox (Vulpes vulpes) in Species Restoration Technology Institute of Korea National Park Service (KNPS), revealed nodular growths in its ventro-cervical region. The fox was introduced from Young-Yang Gun in 2012 to KNPS for re-introduction of the red fox. It has been cared in captive facility and showed the mass in August 2013 that was sent to Wildlife Medical Center. For the diagnosis of underlying disease and cervical mass, radiographical and sonographical examinations, complete blood count, serum chemistry analysis, peripheral blood smear examination and surgical removal of the mass were performed. The mass was fixed in 10% neutral buffered formalin and processed routinely for haematoxylin and eosin (HE) stain. Based on hematological and serum chemical examination, the fox showed mild leukocytosis, thrombopenia, increase of creatine kinase MB (CKMB) and uric acid. However, it was considered as no clinical relevance since the fox showed no related clinical signs. Macroscopically, the mass was round shape, whitish and well-demarcated. Microscopically, it was diagnosed as a lipoma consisting of mature adipose tissue. Lipoma is a common benign tumor in most domestic animals, however it has never been reported in the red fox. The present case report provides comprehensive diagnosis of a subcutaneous lipoma in a red fox.

Microbiological Hazard Analysis of Ginseng Farms at the Cultivation Stage to Develop a Good Agricultural Practices (GAP) Model (인삼의 GAP 실천모델 개발을 위한 재배단계의 미생물학적 위해도 평가)

  • Shim, Won-Bo;Kim, Jeong-Sook;Chung, Duck-Hwa
    • Journal of Food Hygiene and Safety
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    • v.28 no.4
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    • pp.312-318
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    • 2013
  • This study validated microbiological hazards of ginseng farms at the cultivation stage and suggested recommendations to develop a good agricultural practices (GAP) model. A total of 96 samples were collected from cultivation environments (soil, irrigation water, and atmosphere), plants (ginseng and its leaf), personnel hygiene (glove, cloth, and hand) of 3 ginseng farms (A, B, and C) and were tested to analyze sanitary indicator bacteria (aerobic plate count, coliforms and Escherichia coli), major foodborne pathogens (E. coli O157:H7, Listeria monocytogenes, Salmonella spp., Staphylococcus aureus, and Bacillus cereus), and fungi. Total bacteria, coliform, and fungi in the 3 ginseng farms were detected at the level of 1.3~6.0, 0.1~5.0, and 0.4~4.9 v/g (or mL, hand, and $100cm^2$), respectively. Only irrigation water collected from one ginseng farm was confirmed to be E. coli positive. In case of pathogenic bacteria, B. cereus was detected at levels of 0.1~5.0 log CFU/g (or mL, hand, and $100cm^2$) in all samples, but other pathogen bacterias were not detected in any samples from all farms. Although E. coli were detected in irrigation water, the level of microbial for the three farms was lower than the regulation limit. According to the results, the ginsengs produced from the 3 farms were comparatively safe with respect to microbiological hazard. However, cross-contamination of bacteria from environments and workers to ginseng has been considered as potential risks. Therefore, to minimize microbial contamination in ginseng, GAP model should be applied for ensuring the safety of ginsengs.

A Study on the Types of Old County-Maps in the Case of Dongrae-Bu(동래부) (조선 후기 군현지도의 유형 연구 - 동래부를 사례로 -)

  • Kim Kihyuk;Yoon Yongchul;Bae Miae;Jung Am
    • Journal of the Korean Geographical Society
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    • v.40 no.1 s.106
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    • pp.1-26
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    • 2005
  • This paper is to classify old county-maps of Dongrae-bu(東萊府), Busan in late-Chosun dynasty and to analyze place names in maps comparatively. 26 maps covering Dongrae-bu were collected from the old county-map atlas(郡縣地圖帖) and Eupji(邑誌). By e comparative analysis of contents, those maps can be classified into four types. The first type(named 'Haedong-Jido(海東地圖)') included 7 county-maps in which information of military, and administrative contents are mainly mapped. The second type(named 'Yeongnam-do(嶺南地圖)') included 5 coon maps in which information about beacon routes and road systems were regarded as very important. The third type(named 'Grid-system map(方眼式地圖)') included 4 county-maps which were drawn as same scale with 20-ri(理) grids. The fourth type(named 'Local Count-map(地方郡懸地圖)') included 7 county maps which were drawn by local mappers. Comparative analysis of place names between those four types revealed that Grid-system maps were developed toward the large scale whole map of Korea in the 19th century.

Estimation of Pollutants Loading from Non-Point Sources Based on Rainfall Event and Land use Characteristics (강우강도와 토지이용을 고려한 비점오염물질 부하량 산정에 관한 연구)

  • Lee, Hye-Won;Choi, Nam-Hee;Lee, Yong-Seok;Choi, Jung-Hyun
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.8
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    • pp.572-577
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    • 2011
  • The unit load has simply been used to estimate total pollutant loading from non-point sources, however, it does not count on the variable pollutant loading according to land use characteristics and rainfall intensity. Since pollutant emission from the watershed is strongly dependent on the rainfall intensity, it is necessary to find out the relationship between pollutant loading and rainfall intensity. The objective of this study is to develop simple and easy method to compute non-point source pollution loads with consideration of rainfall intensity. Two non-point source removal facility at Gyeongan-dong (Gwangju-si) and Mohyeon-myeon (Yongin-si), Gyeonggi-do was selected to monitor total rainfall, rainfall intensity, runoff characteristics and water quality from June to November, 2010. Most of Event Mean Concentrations (EMC) of measured water quality data were higher in Gyeongan which has urban land use than in Mohyeon which has rural land use. For the case of TP (Total Phosphorus), Mohyeon has higher values by the influence of larger chemical uses such as fertilizer. The relationship between non-point source pollution load and rainfall intensity is perfectly well explained by cubic regression with 0.33~0.81 coefficients of determination($R^2$). It is expected that the pollution loading function based on the long-term monitoring would be very useful with good accuracy in computing non-point source pollution load, where a rainfall intensity is highly variable.

The Study on Decomposition of Metal-working Fluids Against Microbes (미생물을 이용한 절삭유제의 부패성능 평가에 관한 연구)

  • Kim, Young-Wun;Hong, Kwang Min;Chung, Kunwo;Park, Chan-Jo
    • Korean Chemical Engineering Research
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    • v.44 no.4
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    • pp.350-355
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    • 2006
  • Growth curves of microbes were examined to evaluate decomposition of metal-working fluids and decomposition properties of metal-working fluids were experimented using controled microbes such as E. coli and K. pnemoniae. According to the results of growth curve of microbes, the growth period depended on species of microbes, 2 h of E. coli, 3 h of K. pneumoniae, 4 h of P. aeruginosa and 3 h of P. oleovarans after incubation. The colony count of E. coli and K. pneumoniae controled to OD of 0.5 ranged from $4.4{\sim}10.0{\times}10^5CFU/mL$ and $1.8{\sim}9.5{\times}10^7CFU/mL$, respectively. The decomposition of metal-working fluids was excellently progressed in the range of pH 6~8 than below pH 4 and above pH 10. In the case of controled fluids to pH 6~8, the decomposition of the fluid containing ester group was more accelerated than that of the fluid containing ethylene glycol.

The Study of Radon Activity and Uranium Concentration of Ground Water and Surface Water (지하수와 지표수에서의 라돈 및 우라늄의 실태 조사)

  • Oh, Youngmi;Lee, Jongbok;Shin, Kyungjin;Kim, Hakchul;Lee, Jaehee;Hwang, Sangchul;Jung, Sanggi;Lee, Sangtae
    • Journal of Korean Society on Water Environment
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    • v.23 no.2
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    • pp.201-205
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    • 2007
  • This paper reports the amount of $^{222}Rn$ and $^{238}U$ in 18 sites of ground water and 30 sites of surface water. The instrument used to count $^{222}Rn$ activity was the liquid scintillation counter (LSC) which could resolute ${\alpha}$ and ${\beta}$ radiations. And $^{238}U$ was analyzed by the inductively coupled plasma (ICP). Radon and Uranium were not detected in raw and treated water which were sampled in a water treatment plant. However, radon ($^{222}Rn$) was high concentration in ground water from Jeon-la, Gang-won. So was uranium ($^{238}U$) in case of ground water from Gang-won, Choong-chung. Radon ($^{222}Rn$) activities were detected less than 15 pCi/L at 5 sampling points, 15~300 pCi/L at 7 sampling points, 300~4000 pCi/L at 6 sampling points. However, Radon ($^{222}Rn$) activities of all ground water samples were less than 4,000 pCi/L, which was bellow American Alternative Maximum Contamination Level (AMCL). Uranium ($^{238}U$) concentrations were less than $0.1{\mu}g/L$ at 5 sampling points, from $0.1{\mu}g/L$ to $20{\mu}g/L$ at 13 sampling points. Uranium was not detected in about 30% of the whole samples, but the concentration ranged from relatively low to high concentrations depending on the sampling point. The minimum detectable activity (MDA) of radon was 15 pCi/L. and the detection limit of uranium was $0.1{\mu}g/L$.

The Peak Systolic Pressure-End Systolic Volume Index Relation in Patients with Coronary Artery Disease Using Radionuclide Gated Blood Pool Scintigraphy (관상동맥질환에서 심장풀스캔을 이용한 최고수축기혈압-수측기말용적곡선의 분석)

  • Kim, Byung-Tae;Koong, Sung-Soo;Bom, Hee-Seung;Chung, June-Key;Park, Young-Bae;Lee, Myung-Chul;Lee, Young-Woo;Koh, Chang-Soon
    • The Korean Journal of Nuclear Medicine
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    • v.21 no.2
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    • pp.175-182
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    • 1987
  • For measurement of ventricular performance, ejection fraction (EF) has gained wide acceptance. But EF is influenced not only by changes in muscle function but also by changes in cardiac loading conditions. In case of valvular heart disease which is variable in loading conditions, EF cannot be reliable as an index of myocardial contractility. The end systolic pressure (ESP)-end systolic volume (ESV) relation, howver, is known to represent myocardial contractility, independent of changes in loading conditions. Similar results can be obtained by using peak-systolic pressure (PSP) instead of ESP. To evaluate the utility of the peak systolic pressure-end systolic volume index (PSP-ESVI) relation as an index of myocardial function, we measured $PSP&ESVI$ in 19 partents with coronary artery disease before $(PSP_1\;&\;ESVI_1)$ and after $(PSP_2\;&\;ESVI_2)$ sublingual administration of nitroglycerin. PSP was measured with standard mercury sphygmomanometer during gated blood pool scintigraphic study. ESVI was measured by count derived method after attenuation correction. $PSP_2\;&\;ESVI_2$ measurement was started when the fall of PSP was greater than 5 mmHg after 7-14 minutes post-administration of nitroglycerin. Mean values $({\pm}S.D.)$ of $PSP_1\;&\;ESVI_1$ was $124.9({\pm}20.7)mmHg\;&\;59.4({\pm}39.9)ml/M^2$. Mean values $({\pm}S.D)$ of $PSP_2\;&\;ESVI_2$, was $113.2({\pm}19.9)mmHg\;&\;37.5({\pm}26.1)ml/M^2$. There was a significant difference between mean values of $PSP_1\;&\;PSP_2$, (p<0.01), and mean values of $ESVI_1\;&\;ESVI_2$, (p<0.01). $PSP_1-PSP_2/ESV_1-ESVI_2,\;PSP_1/ESVI_1$ and EF were in the range of 0.14-5.19 mmHg/ml/$M^2$, 0.67-7.68 mmHg/ml/$M^2$ and 10.8%-74.5% respectively. $PSP_1-PSP_2/ESVI_1-ESVI_2$, and EF showed exponential correlation (r=0.85, P<0.01). The correlation coefficient between $PSP_1/ESVI_1$ and EF was 0.73(p<0.01). With the above results, we suggest that $PSP_1-PSP_2/ESVI_1-ESVI_2$, and $PSP_1/ESVI_1$, can be used as an index of myocardial function.

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Changes of Chemical Composition and Microflora in Commercial Kimchi (시판 김치의 발효 온도별 성분과 미생물 변화)

  • Shin, Dong-Hwa;Kim, Moon-Sook;Han, Ji-Sook;Lim, Dae-Kwan;Bak, Wan-Soo
    • Korean Journal of Food Science and Technology
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    • v.28 no.1
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    • pp.137-145
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    • 1996
  • Chemical changes, lactic acid bacteria and yeast counts in kimchi prepared by a commercial manufacturer in large scale were monitored at different fermentation temperature. The optimum pH of kimchi, around pH 4.2, reached within 2 days at $25^{\circ}C$, 3 days at $15^{\circ}C$ and 23 days at $5^{\circ}C$ fermentation, respectively. The optimum acidity calculated as lactic acid was not exactly coincident with pH. The total viable count reached at maximum within 2 days at $25^{\circ}C$, 6 days at $15^{\circ}C$ and 12 days at $5^{\circ}C$ fermentation, respectively. The identified strains of Lactobacilli during kimchi fermentation were L. brevis, L. plantarum and L. acidophilus with 3 unidentified strains. L. brevis, L. plantarum appeared from the first stage of fermentation to the terminal at $15^{\circ}C$ and $25^{\circ}C$ with keeping a constant level of viable number. In case of Leuconostoc species, L. mesenteroides subsp. mesenteroides was identified. This strain increased in viable number at the beginning of fermentation and dropped sharply at all fermentation temperatures. Pediococcus species including P. pentosaceus and one unidentified strain increased at the first stage of fermentation and decreased after on. Streptococcus faecium subsp. casseliflavus which appeared at the middle stage and Aerococcus viridans which was sole strain were also confirmed during kimchi fermentation. Cryptococcus laurenti was identified at all fermentation temperature and disappeared at the first stage of fermentation. It was reappeared 10 days only after fermentation at $25^{\circ}C$.

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A novel BTK gene mutation, c.82delC (p.Arg28 Alafs*5), in a Korean family with X-linked agammaglobulinemia

  • Lee, Jeongeun;Rhee, Minhee;Min, Taek Ki;Bang, Hae In;Jang, Mi-Ae;Kang, Eun-Suk;Kim, Hee-Jin;Yang, Hyeon-Jong;Pyu, Bok Yang
    • Clinical and Experimental Pediatrics
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    • v.59 no.sup1
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    • pp.49-52
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    • 2016
  • X-linked agammaglobulinemia (XLA) is a hereditary humoral immunodeficiency that results from Bruton's tyrosine kinase (BTK ) gene mutations. These mutations cause defects in B-cell development, resulting in the virtual absence of these lymphocytes from the peripheral circulation. Consequently, this absence leads to a profound deficiency of lg all isotypes, and an increased susceptibility to encapsulated bacterial infections. A 15-month-old Korean boy presented with recurrent sinusitis and otitis media after 6 months of age, and had a family history of 2 maternal uncles with XLA. Laboratory tests revealed a profound deficiency of Ig isotypes, and a decreased count of $CD19^+$ B cells in the peripheral circulation. Based on his family history and our laboratory test results, he was diagnosed with XLA. We performed BTK gene analysis of peripheral blood samples obtained from family members to confirm the diagnosis. Mutational analysis revealed a novel hemizygous frameshift mutation (c.82delC, p.Arg28Alafs*5), in the BTK gene. His mother and maternal grandmother were heterozygous carriers of this mutation and his two maternal uncles were hemizygous at the same position. After XLA diagnosis, intravenous immunoglobulin (400 mg/kg, monthly) treatment was initiated; recurrent sinusitis and otitis media were subsequently brought under control. To our knowledge, this is the first reported case of a Korean pedigree with a novel mutation in the BTK gene.