• 한국물환경학회지
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• 제32권6호
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• pp.520-527
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• 2016

Phycocyanin (PC) is one of the water-soluble accessory pigments of cyanobacteria species, and its concentration is used to estimate the presence and relative abundance of cyanobacteria. In laboratory experiments, PC content of field data were determined using Sarada's freeze-thaw method in algal bloom season. The effectiveness of three selected extraction methods (repeated freeze-thaw method, homogenization, power control) for PC were determined. The extraction efficiency of phycocyanin was the highest (of the methods compared) when a single freezing-thawing cycle was followed by pre-sonication. Applying this optimized method to surface water of Korean inland waters, the average concentration distribution was estimated at $2.9{\sim}51.9mg/m^3$. It has been shown that the optimized pre-sonication method is suitable to measure cyanobacteria PC content for the characterization of inland waters. The approach and results of this study indicates the potential of effective methods for remote monitoring and management of water quality in turbid inland waters using hyperspectral remote sensing.

• 한국도로학회논문집
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• 제15권4호
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• pp.65-73
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• 2013

PURPOSES : As a research to develop a cement treated base course for an airport pavement which can enhance its drainage, this paper investigated the strength, infiltration performance and durability of the pervious concrete with respect to maximum coarse aggregate sizes and compaction methods. METHODS : This study measured compressive strength, infiltration rate, continuous porosity and freeze-thaw resistance of pervious concrete specimens, which were fabricated with five different compaction methods and different maximum aggregate sizes. In addition, in order to reduce the usage of Portland cement content and to enhance environment-friendliness, a portion of the cement was replaced with Ground Granulated Blast Furnace Slag (GGBS). RESULTS: Compressive strength requirement, 5 MPa at 7 days, was met for all applied compaction methods and aggregate sizes, except for the case of self-compaction. Infiltration rate became increased as the size of aggregate increased. The measured continuous porosities varied with the different compaction methods but the variation was not significant. When GGBS was incorporated, the strength requirement was successfully satisfied and the resistance to freezing-thawing was also superior to the required limit. CONCLUSIONS: The infiltration rate increased as the maximum size of aggregate increased but considering construct ability and supply of course aggregate, its size is recommended to be 25mm. With the suggested mix proportions, the developed pervious concrete is expected to successfully meet requirements for strength, drainage and durability for cement treated base or subbase course of an airport pavement.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.108-108
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• 2003

The purpose of this is to investigate the effects of vitrification in open pulled straws (OPS) on in vitro survival of porcine embryos. Blastocysts were produced by in vitro fertilization of slaughterhouse-derived, in vitro matured oocytes with frozen-thawed boar semen, and subsequent culture on granulosa cell monolayer. After frozen-thawing, embryos were culture in NCSU-23 medium with 5 mM hypotaurine, 4 mg/$m\ell$ BSA and 10 ng/$m\ell$ for 48 hrs to survival tests. When blastocysts were frozen-thawed by OPS methods, the embryos with normal morphology were 32.1, 34.5 and 38.9 % in early blastocyst, blastocyst and expanded blastocyat stages. The rates of partial damaged embryos were significantly (P<0.05) higher in early biastocysts than expanded blastocysts. In another experiment, the embryos frozen by OPS methods were cultured for 48 hrs for survival and developmental rates in vitro. The proportions of embryos hatched were 11.8, 20.2 and 33.3% in embryos frozen-thawed at stages of early blastocyst, blastocyst and expanded embryos. On the other hand, The proportions of embryo with normal morphology after culture were 23.5, 25.0 and 33.3% in embryos frozen-thawed at stages of early blastocyst, blastocyst and expanded embryos. These finding indicate the possible broader application for OPS methods that this procedure described is relatively harmless, that it can be used for blastocysts of different developmental stages.

• 정보과학회 컴퓨팅의 실제 논문지
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• 제22권1호
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• pp.8-19
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• 2016

도로의 결빙과 해빙으로 도로면의 수축과 팽창이 반복되어 도로면에서 침투한 수분이 포장면의 결합력을 약화시켜 노면홈(포트홀)을 발생시킨다. 현재의 포트홀 조사는 현장에서 육안 조사하고 기록하는 수동적인 방식으로 매년 수 만개소의 포트홀이 발생하는 것에 어려움이 발생하고 있다. 포트홀 정보를 자동으로 수집하기 위해 최근까지 가속도 센서를 이용한 기술과 레이저 스캐닝을 이용한 기술이 많이 연구되었다. 하지만, 가속도 센서 기반 기술은 낮은 인식률과 제한된 센싱 영역의 문제가 있고, 레이저 스캐닝 기반 기술은 비용이 너무 큰 문제가 있다. 따라서, 본 논문에서는 대중적으로 사용하는 차량용 블랙박스 카메라를 이용한 자동 포트홀 탐지 기술을 제안한다. 일반적으로 차량용 블랙박스 카메라에 탑재한 연산프로세서는 낮은 컴퓨팅 능력을 가지므로 포트홀 탐지 알고리즘을 그게 맞게 설계할 필요가 있다. 설계된 알고리즘을 블랙박스에 내장하여 도로 주행실험을 실시하며, 포트홀 탐지 성능을 중심으로 한 실험결과는 포트홀 탐지 정밀도, 민감도 등의 지표를 토대로 분석하고, 실시간 포토홀 탐지 기술의 현장 적용성을 확인한다.

• 한국식품위생안전성학회지
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• 제13권4호
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• pp.441-447
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• 1998

본 연구는 조리과정 중에 Salmonella 식중독균수가 어떻게 변화되는지를 관찰하고, 이를 토대로 HAPPC model 설정을 위한 실증 자료를 제공하고자 수행되었다. 돼지고기(크기: $0.5cm\;{\times}\;10cm\;{\times}\;10cm$, 무게 : 약 25g) 에 살모넬라균 (S. typhimurium)을 $10^{7}\;CFU/g$으로 오염시키고 조리하는 과정(해동 방법, 해동기간 및 조리방법)에서 균수의 증가 또는 감소를 관찰하였으며, 오염된 돼지고기를 사용하여 한국인이 일상적으로 많이 먹는 돼지고기 음식(잡채)을 만드는 모의실험을 통하여 이 음식물에서 최종적인 균수를 측정하였다. 해동 방법별로는 24시간 후에 균수가 냉장 온도 ($4~10^{\circ}C$)에서 $10^{10}\;CFU/g$, 실온($22~29^{\circ}C$)에서 $10^{21}\;CFU/g$의 수준으로 증가하였다. 전자레인지에 의한 급속 해동(40초)에서는 균수가 가장 낮게 측정되었으나 $10^{8}\;CFU/g$으로 역시 증가하였다. 해동 기간별로 냉장과 실온에서의 균수 변화를 보면 균수는 시간이 경과될수록 뚜렷이 증가를 나타내었다. 냉장온도에서 해동시에는 24시간 후에 1010CFU/g , 48시간 후에 1013CFU/g, 72시간 후에 $10^{20}\;CFU/g$의 수준으로 증가하였다. 실온 해동시 2시간 후에는 $10^{10}\;CFU/g$ 수준, 4시간 후에슨 $10^{15}\;CFU/g$ 수준, 8시간 후에는 $10^{16}\;CFU/g$ 수준, 12시간 후에슨 $10^{18}\;CFU/g$ 수준, 그리고 24시간 후에는 $10^{21}\;CFU/g$ 수준으로 증가하였다. 조리방법별로는 돼지고기를 볶기 ($150{\pm}7^{\circ}C$, 3분) 한 결과 균수는 $10^{6}\;CFU/g$ 수준으로 초기보다 약간 감소하였다. 삶기(20분) 한 결과 $60^{\circ}C$에서는 초기와 같은 $10^{7}\;CFU/g$ 수준, $63^{\circ}C$에서는 $10^{6}\;CFU/g$ 수준으로 볶기에서의 균수와 같게 나타났으며, $65^{\circ}C$에서는 $10^{4}\;CFU/g$ 으로 감소하였다. S. typhimurium에 오염된 돼지고기를 위와 같이 볶은것($10^{6}\;CFU/g$)을 사용하여 잡채를 만든 결과 (소요시간 :10{\pm}2분)균수가 $10^{7}\;CFU/g$으로 증가하여 Salmonella 식중독의 발생 위험성이 더욱 커진 것으로 나타났다. 이러한 결과로 볼 때 돼지고기에서는 S. typhimurium의 증식은 조리과정에 의하여 영향을 받는 것을 알 수 있다. 식중독을 야기할 수 있는 수준으로 오염된 돼지고기를 조리할 때에는 $65^{\circ}C$에서 20분 이상 삶아야만 식중독 발생 예방이 가능한 것으로 사려되었다. 또한 이상과 같은 결과로부터 이 잡채에 대한 위해분석(HA)에서 원재료 고기의 초기 미생물 오염도가 위해요소(hazard)로 지적되며, 부적절한 조리단계(조리방법 및 조리온도와 시간)과 중점관리점(CCP)으로 판명된다. 잡채의 경우 돼지고기를 볶아서 사용하는 경우가 많으므로 이 조리방법의 표준화(고기의 크기와 양에 따른 온도/시간/조리방법)가 필요하다.

• 한국수정란이식학회지
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• 제27권4호
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• pp.259-264
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• 2012

Solution of glycerol, ethylene glycol, sucrose, dextrose (GESD) and cryotop methods were carried out to investigate the survivability on vitrification of embryos. Embryos cultured in vitro were vitrified by GESD of 10 or 8 step and cryotop methods of 6 step, from cryopreservation step to frozen-thawed and culture step. Survival rate and ICM, TE cells of embryos were investigated after frozen-thawed 24 h. As a results, cryotop method was significantly (p<0.05) higher ($85.76{\pm}5.3$ vs. $66.71{\pm}2.4$, $44.80{\pm}2.1%$) than GESD 10 or 8 step methods on survivability. Also, In ICM cell number, cryotop method was significantly (p<0.05) higher to $45.67{\pm}4.7$ cells than GESD 8 step method. TE cell number was significantly (p<0.05) highest to $111.00{\pm}11.0$ cells in cryotop method. On the other hand, survival rate, TE and total cell number were all the significantly (p<0.05) high, except ICM in GESD 10 step method between GESD 10 step method and GESD 8 step method. In conclusion cryotop method was to be most effective, but it is considered necessary to study vitrification method for step-by-step freezing and thawing process.

• 대한영양사협회학술지
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• 제11권1호
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• pp.105-113
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• 2005

The purpose of the study was to evaluate the perception of importance about HACCP guidelines of dietitians. A questionnaire was developed to achieve research objectives and sent to random samples of 500 dietitians by a mail ; 418(84%) responses were analyzed. The respondents used three-point-scale to rate their perception of importance about HACCP from 1-"will be necessary" to 3-"very important". All statistical analyses were conducted using SAS package(version 8.12) for Windows. Means and standard deviations were computed for all variables. One-way analysis of variance and Duncan's multiple range test were used to assess differences in the perception of importance about HACCP guidelines among business and industry, health care and school foodservice managers. The study results were summarized as follows. Dietitians especially had lower perception of importance on the contents of Critical Control Points(CCPs), such as; adequate thawing methods and keeping records(1.95) and correct cleaning and sterilizing for raw vegetables and fruits(1.93). The rates of perception of importance about HACCP guidelines were significantly different from 19 of the 37 contents among business and industry, health care and school foodservice(p<.05 or p<.01 or p<.001). Generally, the item related to purchasing and inspection management had the highest perception level score and the item related to pre-preparation management had the lowest perception level score in foodservice production process(p<.001). Results indicate that there is a need for increased education of dietitians about HACCP principles and appropriate practices.

• 터널과지하공간
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• 제7권2호
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• pp.91-99
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• 1997

To stabilize the energy price, the more storage facilities of energy are required and among the storage methods of LPG and LNG, the method of storage at low temperature under normal confining pressure is considered. It is needed to understand the mechanical and thermal characteristics of rock under temperature variation so that the behaviors of rock can be predicted. In this paper, the variation of the rock charateristics of the Hwangdeung granite and the Boryung sandstone is studied at low temperature. The mechanical characteristics of rock under low temperatures are that as temperature decreased, unaxial compression strength and Young's modulus increased for Hwangdeung granite; strength and Young's modulus in wet condition were greater than those in dry condition. In the case of Boryung sandstone, as temperature decreases unaxial compression strength and Young's modulus increase but decrease below -10$0^{\circ}C$ in dry condition and below -16$0^{\circ}C$ in wet condtion. The mechanical characteristics of rock after cooling to previous temperature and thawing are that uniaxial compression strength and Young's modulus decrease as temperature decreases. Uniaxial compression strength and Young's modulus in wet conditon decrease more than those in dry condition. Brazilian tension strength decreases as temperature decreases.

• 한국수정란이식학회지
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• 제19권1호
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• pp.27-34
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• 2004

This study evaluated the efficiency and compared with different materials of loading vessels for vitrification-plastic/glass, copper grid and nylon. The loading method, vitrification, cryop-reservation and warming method of the oocytes were examined. The loading samples prepared in manual or company-made and sterilized, loaded the COCs selected on each samples and cultured for maturation during 40 hours, and then exposed sequentially to ethylene glycol solution. Thawing method was reversely treated and exposed for warmed oocytes. After oocytes were thawed, fertilized and cultured in vitro for 3-4 hours, rates of development and morphological appearance were examined. The results were as summarized: ㆍOPS from company-made or hand-made of the hematocrit micropipettes, NLS from fishing line and EMG from company-made for EM were used for loading oocytes, respectively. ㆍThe efficiency of freezing method and loading convenience were orderly higher in OPS, NLS and EMG. The optimal capacity per vessel was orderly lowered in NLS, EMG and OPS, respectively. ㆍAfter oocytes were warmed, the recovery rate, morphology and rate of development were orderly higher in OPS, NLS and EMG, respectively. ㆍIn conclusion, OPS has the advantages of achieving a little more survival and preserving results than other two loading methods.

• 콘크리트학회논문집
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• 제12권1호
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• pp.113-125
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• 2000

The purpose of this study was to examine the durability characteristics of controlled low strength material(flowable fill) with high volume fly ash content. Flowable fill refer to self-compacted, cementitious material used primarily as a backfill in lieu of compacted fill. The two primary advantages of flowable fill over traditional methods are its ease of placement and the elimination of settlement. Therefore, in difficult compaction areas or areas where settlement is a concern, flowable fill should be considered. The fly ash used in this study met the requirements of KS L 5405 and ASTM C 618 for Class F material. The mix proportions used for flowable fill are selected to obtain low-strength materials in the 10 to 15kgf/$\textrm{cm}^2$ range. The optimized flowable fill was consisted of 60kg f/$\textrm{m}^3$ cement content, 280kgf/$\textrm{m}^3$ fly ash content, 1400kgf/$\textrm{m}^3$ sand content, and 320kgf/$\textrm{m}^3$ water content. Subsequently, durability tests including permeability, warm water immersion, repeated wetting & drying, freezing & thawing for high volume fly ash-flowable fill are conducted. The results indicated that flowable fill has acceptable durability characteristics.