• 한국농공학회논문집
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• 제52권4호
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• pp.93-101
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• 2010

The purpose of this study is to provide basic data for utilization in environment-friendly and economically outstanding CSG construction method by physical and mechanical properties of CSG materials including characteristics of uniaxial compressive strength, microscopic structure and freezing and thawing resistance in accordance with the cement content and curing time of the cement, and size of specimen. In this study, specimens with cement content of 4, 6, 8 and 10% of the total weight were, and, in order to examine the characteristics of the sizes of specimen, specimens with ${\Phi}50{\times}100mm$, ${\Phi}100{\times}200mm$ and ${\Phi}150{\times}300mm$ were manufactured to assess the features including compressive strength, microscopic structure, freezing and thawing, and degree of wet-dry. As results, it was found that with greater size specimen or contents of cement in the specimen, compressive strength, freezing and thawing resistance, and wet-dry resistance increase. Moreover, reactive products for each size of specimen were examined and it was possible to verify that some typical needle structured ettringite was generated due to blending of cement through microscopic structure analysis such as SEM and EDS analysis.

• 한국신뢰성학회지:신뢰성응용연구
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• 제18권3호
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• pp.260-270
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• 2018

Purpose: The purpose of this study is to evaluate the life of the capsule, which is a core part of the heat storage cooling system. This paper will develop a life test mode that can reproduce environment conditions through the analysis of capsule shrinkage and expansion characteristics. Methods: In order to determine the life test mode of the capsule, this paper analyzed the case of field failures and analyzed the deformation characteristics according to the pressure fluctuation of the capsule. The method to find out whether the field failure and deformation analysis results are consistent is the testing with the construction of the repetition pressure test equipment and the thermal cycle test to reproduce the freezing and thawing characteristics. Results: In this study, failure mode analysis and analysis of freezing and thawing characteristics regarding to the capsule positions were completed. Based on this test & analysis results, this paper have been able to determine the main parameters for determining the life test mode, the freezing and thawing time. Conclusion: Determining the lifetime mode of the capsule can be used to improve the life and performance of the thermal storage system.

• 한국농공학회:학술대회논문집
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• 한국농공학회 1998년도 학술발표회 발표논문집
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• pp.161-166
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• 1998

This experiment was on purpose to estimate freezing and thawing resistance concering with developing rice straw ash concrete which were mixed rice straw ash to cement as ratio of cement weight. Freezing and thawing test was done by Method A of KS F 2456. It could estimate change of original mass, pulse velocity and dynamic modulus of elasticity during test. Test results showed that 5% filled rice straw ash concrete had the highest durability factor(DF) as 86 and from 5% to 7.5% filled rice straw ash concrete showed higher DF than normal cement concrete.

• 한국수정란이식학회지
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• 제10권3호
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• pp.243-250
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• 1995

Frozen storage of the oocytes has been used in a few mammalian species including mouse, hamster, human and cattle. However, frozen4hawed oocvtes show different sperm penetration on the levels of the zona pellucida and the plasma memhrane when compared with fresh oocytes. To elucidate biological changes occurring during freezing and thawing, we examined the kinetics of sperm penetration into frozen-thawed hamster oocytes. Oocytes obtained from superovulated female golden hamsters were frozen-thawed in an autofreezer according to an established method. Fresh and frozen4hawed oocytes were fertilized in vitro with capacitated hamster spermatozoa after removing the zona pellucida. The oocytes were examined at 1, 2, 3 and 6 h postinsemination. Sperm penetration found to be 1 h delayed in frozen-thawed oocytes. Other parameters such as degree of polyspermy and decondensing sperm heads were not affected by freezing and thawing. The results suggest that freezing and thawing may cause changes in the egg membrane surface and subsequently which leads to delay in the sperm-egg fusion.

• 한국방재학회:학술대회논문집
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• 한국방재학회 2008년도 정기총회 및 학술발표대회
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• pp.457-460
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• 2008

CFRP plates has been established as an effective method for rehabilitation and strengthening of concrete structures. The CFRP reinforcements are bonded to beams and slabs using structural adhesives. Adhesive strength can be affected by environmental exposure. During freezing-and-thawing cycling, temperature-induced stresses in the adhesive layer, due to differential thermal expansion between the CFRP and the substrate concrete, may lead to bond damage and contribute to or cause premature CFRP composite separation. This paper presents the results of experimental program undertaken to investigate the effects of freeze-thaw cycling (from -18 to $4^{\circ}C$) on the behavior and failure characteristics of RC beams strengthened in shear with CFRP plate using acoustic emission (AE) technique.

• 한국도로학회논문집
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• 제14권5호
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• pp.57-65
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• 2012

PURPOSES: It is important to consider the long-term performance of concrete pavement, because concrete pavement is more exposed to the various environmental conditions than any other concrete structures. One of the several methods to evaluate the long-term performance of concrete during winter is KS F 2456. Relative dynamic modulus of elasticity shows the resistance to freezing and thawing. METHODS: To measure relative dynamic modulus of elasticity, ultra sonic is generally used. But in this study, to measure the relative dynamic modulus of elasticity, both ultra sonic and shear wave were used and then compared each other. RESULTS: The results from the measurement by ultrasonic wave and shear wave were divided into three types. Type 1 : Specimens are good and relative dynamic modulus of elasticity did not decrease until 300 cycle. Type 2 : The relative dynamic modulus of elasticity decreased from the late cycle.(about 150 cycle later) Type 3 : The relative dynamic modulus of elasticity consistently decreased from the beginning. As a result of ANOVA, there is no difference according to measuring method, in type 2 and 3. But there is a difference according to measuring method, in type 1's relative dynamic modulus of elasticity. CONCLUSIONS: It is proved that shear wave can be used to understand the damage tendency of relative freezing and thawing and to measure the relative dynamic modulus of elasticity.

• Clinical and Experimental Reproductive Medicine
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• 제19권2호
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• pp.163-168
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• 1992

This study was carried out to set up the ovum bank for ovum donation and to determine the best freezing method for human immature oocytes. Human immature follicular oocytes were cryopreserved by slow freezing and rapid thawing method. Immature follicular oocytes were treated by propanediol(PROH) solution by 2 and 4 step method in protocols A & B, respectively. In protocol C, immature oocytes were exposed to sucrose prior to treatment of PROH by 4 step method. We compared survival rate, maturation rate, and fertilization rate of immature oocytes among three protocols. Results were as follows. 1. Oocytes treated by the protocol C showed the highest survival rate( 70.3 %) and maturation rate(34.6%) after thawing. 2. Survival rate of oocytes treated by the protocol C was significantly higher than that of the protocol B after thawing(p<0.05). In conclusion, treatment of oocytes with sucrose prior to expose PROH was the best freezing method. Sucrose may have reduced the toxic effect of cryoprotectant to oocytes. We failed to induce fertilization of oocytes, which were treated by any protocols, by conventional insemination method, but obtained 28.8% fertilization rate by using partial zona dissection(PZD) method. This result suggests that micromanipulation(PZD) of the thawed oocytes before insemination will improve the fertilization rate.

• 식품기술
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• 제22권4호
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• pp.731-741
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• 2009

• 자원리싸이클링
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• 제19권4호
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• pp.35-40
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• 2010

최근에 들어 투수콘크리트의 동결융해에 대한 저항성이 저하되는 것과 줄눈부에서의 골재박리 등의 많은 문제점들이 대두되고 있다. 따라서 본 연구에서는 기존의 투수콘크리트의 내구성을 향상시키기 위한 방안으로 잔골재와 실리카흄, 고성능감수제를 사용하여 강도 및 내구성 증진 방안을 연구하였다. 주요실험인자로 실리카흄 혼입률 10%와 잔골재 혼입률(0%, 7%, 15%)을 선정하고, 압축강도와 휨강도, 투수계수 및 공극률, 동결융해시험을 수행하였다. 실험결과 강도측면에서는 잔골재 혼입률이 증가함에 따라 압축 및 휨강도는 증가하는 경향을 보였으나, 상대적으로 공극률 감소로 인한 투수성은 저하되고, 동결융해 저항성도 크게 감소하는 것으로 나타났다. 따라서 투수콘크리트에 있어서 강도증진을 위해 사용되는 잔골재는 혼입률 제한이 필요하며, 실리카흄 10%이상을 사용하는 것이 강도 및 내구성 측면에서 효율적인 대안이 될 수 있을 것으로 판단된다.

• Clinical and Experimental Reproductive Medicine
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• 제30권2호
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• pp.127-133
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• 2003

Objective: The aim of this study was to investigate the morphological aspects of testicular tissue before and after freezing-thawing by light and transmission electron microscopy. Methods: Tissue biopsies were carried out on mouse testis for freezing. Samples in medium containing 20% glycerol were frozen by computer-controlled freezing program. The effect of freezing-thawing on the structural change of testicular tissues were examined by light and electron microscopy. Results: The freezing-thawing procedure had no significant effect on tubular diameter. However, it caused folding of the lamina propria, and notable damage to Sertoli cells, spermatogonia and spermatocytes. The cells were detached, desquamated from the basal lamina and had increased vacuolization. Round spermatids, elongated spermatids and spermatozoa were less affected, and most of them maintained their normal structure. Conclusions: The structure of spermatogonia, spermatocyte and basal compartments in seminiferous epithelium was significantly altered by freezing-thawing procedure of mouse testicular tissues. Thus, we need to develop a more reliable method for the cryopreservation of testicular tissues.