• 한국지반환경공학회 논문집
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• 제21권3호
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• pp.5-11
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• 2020

최근 도심지에서 많이 발생되는 지반함몰은 교통흐름에 방해가 될 뿐만 아니라 재산적인 손실과 함께 인명피해도 심각하게 발생되는 등 시민들의 안전을 위협하는 요인이 되고 있다. 따라서 함몰된 지반을 긴급하게 복구하여 추가 피해에 대비하여야 하는데 현재 국내의 지반 함몰에 대한 구체적인 기준이 미흡하고 함몰 원인의 정확한 규명 및 재발생에 대한 대책이 미흡한 실정이다. 함몰된 지반의 복구방법으로는 함몰된 흙을 재사용하여 되메우기를 하거나 기타 성토재료를 사용하여 되메움 한 후 도로를 포장하는 방법을 가장 많이 사용하고 있는데 이는 지반함몰을 일시적으로 방지하는 방법에 불과할 뿐 근본적인 해결책으로 볼 수 없다. 또한 이러한 방법으로 보강된 지반은 되메움재의 불량 및 다짐불량 등으로 인하여 추가적인 지반함몰이 발생될 가능성을 배제할 없다. 이 연구에서는 지반 침하로 발생된 지하공동의 복구에 활용할 수 있는 복구재료로써 개량된 준설점토의 공학적 특성을 분석하기 위하여 친환경고화재(EHSM) 및 화강풍화토의 혼합비율을 변화시켜 제작한 공시체에 대한 일축압축강도시험을 수행하였으며, 복구재료의 환경변화에 따른 강도 특성을 규명하기 위하여 동결융해시험을 수행하고 각 단계별 시험이 종료되면 강도분석을 위하여 일축압축강도 시험 및 동탄성시험을 수행하였다. 또한 복구된 지반의 강도특성을 평가하기 위하여 동평판재하시험을 실시하여 복구된 지반의 개량효과를 검증하였다.

• 한국제4기학회지
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• 제18권2호통권23호
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• pp.33-42
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• 2004

In South Korea a Pedo-sedimentary Sequence(PS) indicating the Last Glacial Maximun(LGM) is typified y a brown to dark brown, relatively stiff paleosol layers formed by repetitive freezing and thawing processes which in turn left characteristi glossic textures in soil-solum, polygolnal structures with a flagipans, vertical soil wedges or freezing cracks, and horizontal foliations, As a pre-LGM sedimentary sequences (older than 25Ka), the Old Fluvial Sequence(OFS) overlain by the Slope Sedimentary Sequence(SS) are distributed commonly at the base level higher than 14-15m above present river-bed along the major river basin. After the LGM (ca. 18Ka), the Young Fluvial Sequence(YFS) appears at an altitude ascending order of sedimentary profiles. In this fluvial organic muds of Jangheungri site(Jinju), Sorori site(Cheonwon), and Youngsan estruarine rivermouth(Mokpo) were exemplified in order to interpret their formation ages and environments. As result of $^{14}C$ datings, the formation ages of te organic muds are Boelling to Alleroed (MIS-1). These organic muds were fomed in fluvial backswamp or local pond/bog in response to shifting fluvial system. On the basis of palynological production dominant with Abies/Picea-Betula and Ranunculaceae, Compositae, Cyperaceae, and Graminae, it was interpreted that more boreal to subboreal condition was prevailed rather than temperate like today during the formation of organic muds and soil moisture condition was a repetition of wet and dry condition.

• Reproductive and Developmental Biology
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• 제30권4호
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• pp.279-285
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• 2006

Vitrification has been suggested to be an effective method for the cryopreservation of human ES cells. However, the efficiency of vitrification with different vehicles remains a matter of ongoing controversy. The objective of this study was to assess the efficiency of cryopreservation in human ES cells by vitrification using different vehicles. A human ES cell line and a variety of vehicles, including micro-droplet (MD), open-pulled straw (OPS) and electron microscopic grid (EM-grid), were employed in an attempt to assess vitrification efficiency. In order to evaluate the survivability and the undifferentiated state of the post-vitrified human ES cells, we conducted alkaline phosphatase staining and characterization via both RT-PCR and immunofluorescence assays. The survival rates of the post-vitrified human ES cells using MD, OPS and EM-grid were determined to be 61.5%, 66.6% and 53.8%, respectively. There also exist significant differences between slow-freezing and vitrification (p<0.01). However, no significant differences were detected between the vehicle types. Finally, the pluripotency of human ES cells after thawing was verified by teratoma formation. Cryopreservation using vitrification is more effective than slow-freezing, and the efficiency of vehicles proved effective with regard to the preservation of human ES cells.

• 한국지반신소재학회논문집
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• 제8권1호
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• pp.53-59
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• 2009

본 연구에서는 변형된 동결-융해 시험장치를 이용하여 동상민감성흙의 동결-융해 과정시 지반공학적 특성을 평가하였다. 흙시료의 정상상태에서 동상이 발생되는 양은 전체 동상량의 70~80% 정도로 정상상태의 동상이 일어나기 위한 열흐름 조건을 제안하였다. 즉, 동결시 동결전선에서 순열흐름을 0으로 하기 위해서 열적 경계조건을 변화시키는 방법이다. 제안된 방법으로 변형된 동결-융해 시험의 결과에 대하여 적합성을 평가하였다. 또한, 동상 발현중 제안된 방법을 적용 결과, 장기적인 동상현상을 제시하였으나, 장기적 동상은 매우 작은 동상량이 나타나기 때문에 공학적 요소에서는 무시할 수 있는 동상으로 판단하였다.

• 대한수의학회지
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• 제35권3호
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• pp.635-641
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• 1995

The purpose of this study was to investigate the effects of developmental stage and equilibration time on survival of rabbit embryos following freezing by vitrification. Adult New Zealand White female rabbits were superovulated with PMSG and hCG. The 8-cell stage embryos were collected from 40 to 45 hours after hCG injection by flushing oviducts with Dulbecco's phosphated buffered saline and in vitro cultured in TCM-199 containing 10% fetal calf serum(FCS). Each embryos developed in vitro to 16-cell, compact morula and blastocyst was cryopreserved and cultured following thawing to examine their developmental potential to expanded blastocyst stage in vitro. The frozen-thawed-cultured embryos were stained with Hoechst 33342, and their nuclei were counted using a fluorescence microscope. On the toxicity test of EFS solution as cryopreservation, the survival rates of 8-cell stage embryos was decreased in reverse to increasing of exposure time over 5 minutes. The post-thaw survival rates of embryos on equilibration times was significantly(P<0.05) higher for 2 min. than for 5 or 10 minutes. From morula to blastocyst of rabbit embryos was more suitable than 8-cell stage for cryopreservation by vitrification. The higher post-thaw survival rate of embryos can be achieved by keeping the cryoprotectant at $4^{\circ}C$ than at $20^{\circ}C$. The mean number of nuclei per embryo following freezing by vitrification and in vitro culture to expanded blastocyst at compacted morula and blastcyst was not significantly differ from fresh blastocyst.

• Clinical and Experimental Reproductive Medicine
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• 제49권2호
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• pp.117-126
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• 2022

Objective: This study examined whether the addition of triple antioxidants (3A)-10 µM acetyl-L-carnitine, 10 µM N-acetyl-L-cysteine, and 5 µM α-lipoic acid-in freezing-thawing medium during human sperm cryopreservation using the sucrose vitrification (SuV) and liquid nitrogen vapor (Vapor) techniques could improve post-thaw survival of spermatozoa. Methods: We analyzed 30 samples from healthy human sperm donors. Each sample was allocated into one of five groups: fresh control, SuV, SuV+3A, Vapor, and Vapor+3A. The sperm motility, morphology, viability, intracellular and extracellular reactive oxygen species (ROS) levels, and sperm DNA fragmentation (SDF) were evaluated. Results: The cryopreserved spermatozoa had significantly reduced percentages of motility (p<0.05) and viability (p<0.05). Antioxidant supplementation non-significantly improved these parameters (p>0.05). No significant differences were found in sperm morphology between the fresh and frozen-thawed groups (p>0.05). After freezing, the extracellular ROS levels in the frozen-thawed groups were significantly higher (p<0.05) than in the fresh group. However, we did not find any differences in intracellular ROS parameters among these groups (p>0.05). The SDF was higher in the SuV and Vapor groups than in the fresh group, but without statistical significance (p=0.075 and p=0.077, respectively). Conclusion: Cryopreservation had detrimental effects on sperm motility, viability, and extracellular ROS levels, without changing the morphology or intracellular ROS levels. Antioxidant supplementation was slightly effective in preventing SDF in frozen-thawed spermatozoa.

• 농업과학연구
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• 제13권1호
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• pp.82-89
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• 1986

본(本) 실험(實驗)은 hamster 수정란(受精卵)의 동결보존시(凍結保存時) 가장 적합(適合)한 동결(凍結)및 융해온도(融解溫度)를 구명(究明)하기 위(爲)하여 실시(實施)하였다. 수정란(受精卵)은 hamster 두당(頭當) 30 IU의 PMSG를 복탈내(腹脫內)에 투여(投與)하고 4일후(日後)에 교미(交尾)를 시켰으며 교미(交尾) 3일후(日後)에 도살(屠殺)하여 자궁(子宮)과 난관(卵管)을 관류(灌流)하여 회수(回收)하였다. 회수(回收)된 수정란(受精卵)의 동결(凍結)에 사용(使用)한 보존액(保存液)은 Dulbecco's phosphate buffered saline(PBS)이었으며, 동해방지제(凍害防止劑)로는 Dimetyl sulfoxide (DMSO)로서 3단계(段階)로 최종농도(最終濃度)가 1.5M이 되게 하였다. 수정란(受精卵)의 동결방법(凍結方法)은 실온(室溫)에서 $-6^{\circ}C$까지 $1^{\circ}C/min$의 속도(速度)로 하강(下降)시켜 수직(水植)하고 3 분간(分間) 방치(放置)시킨 다음 $0.30^{\circ}C/min$ 속도(速度)로 $-35^{\circ}C$까지 냉각(冷却)시켰으며, 다음에 $-70^{\circ}C$까지는 $0.1^{\circ}C/min$, $1^{\circ}C/min$ 및 $10^{\circ}C/min$의 3 처리(處理)로 냉각(冷却)시킨후 $-196^{\circ}C$의 액체질소(液體窒素)에 보존(保存)하였다. 융해(融解)는 $4^{\circ}C/min$과 $12^{\circ}C/min$의 속도(速度)로 $37^{\circ}C$ 까지 가온(加溫)하는 방법(方法)과 $37^{\circ}C$의 water bath에서 2 분간(分間) 침지(沈漬)시키는 방법(方法)을 이용(利用)하였다. 평균배란점(平均排卵點)은 35.1개(個)인데 대하여 회수(回收)된 수정란(受精卵)은 27.0개(個)로서 회수율(回收率) 77.0% 였으며, 수정란(受精卵)중에서 8세포기(細胞期) 배(胚) 24.8개(個)로서 그 비율(比率)은 70.6%였다. 수정란(受精卵)의 동결속도(凍結速度)에 따른 생존율(生存率)은 $0.1^{\circ}C/min$으로 동결(凍結)했을 때 55.5~67.7%, $1^{\circ}C/min$에서는 58.8~64.9%, $10^{\circ}C/min$에서는 40.5~44.7%였는데 $10^{\circ}C/min$의 속도(速度)로 동결(凍結)한 것이 유의(有意)하게 나쁜 성적(成績)이었다. 융해방법(融解方法)에 따른 수정란(受精卵)의 평균생존율(平均生存率)은 $4^{\circ}C/min$에서 53.5%, $12^{\circ}C/min$에서 53.7%, $37^{\circ}C$ water bath 융해(融解)에서 59.1%를 나타내어 water bath 융해(融解)가 가장 좋은 성적(成績)이었으나 큰 차이(差異)는 인정(認定)되지 않았다. 본(本) 실험(實驗)의 결과(結果)를 종합(綜合)해 볼 때 hamster 난자(卵子)의 동결(凍結)에는 실온(室溫)에서 $-6^{\circ}C$까지는 $1^{\circ}C/min$, $-35^{\circ}C$까지 $0.3^{\circ}C/min$, $-70^{\circ}C$까지 $0.1^{\circ}C/min$ 또는 $1^{\circ}C/min$의 동결속도(凍結速度)를 유지(維持)하고, $37^{\circ}C$의 water bath에서 2 분간(分間) 침관(沈漬)하여 융해(融解)하는 것이 가장 높은 생존율(生存率)을 얻었다.

• 한국가축번식학회지
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• 제24권4호
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• pp.439-449
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• 2000

본 연구에서는 정자와 외래유전자인 EGFP 유전자를 공배양한 후 정자직접 주입술로 난자를 수정시켜 EGFP 유전자의 발현을 조사하였다. 정자는 외래유전자의 도입이 용이하도록 동결융해, 0.03% Tween-20과 0.02%의 Triton X-100의 처리를 통하여 정자두부의 원형질막을 제거하여 공시하였다. 수정된 난자는 소 난관상피세포가 포함된 CR1aa 배양액에서 공배양을 통하여 체외발달시켰으며, 난자의 발달에 따라 EGFP 유전자의 발현을 형광 현미경 하에서 조사하였다. 원형질막이 제거된 정자로부터 수정란의 정상수정을 확인하기 위하여 18시간째 2PN 2PB를 조사한 결과, 발생율은 각각 DTT 처리구 44.6%, DTT와 Twen-20 처리구 48.4%, DTT와 동결융해 처리구 44.4%, 그리고 DTT와 Triton X-100 처리구 42.9%였다. 수정란의 초기 배 분할율은 DTT 처리구 49.1 %, DTT와 Tween-20 처리구 58.5%, DTT와 동결융해 처리구 43.9% 그리고 DTT와 Triton X-100 처리구 48.4%였으며, 배반포 형성율은 DTT 처리구 10.2%, DTT와 Tween-20 처리구 13.0%, DTT와 동결융해 처리구 6.8% 그리고 DTT와 Triton X-100 처리구 6.5%였다. 이들 발달된 수정란 중 도입된 EGFP 유전자의 발현율은 DTT 처리구 3.8%, DTT와 Tween-20 처리구 11.1%, DTT와 동결융해 처리구 13.8% 그리고 DTT와 Triton X-100 처리구 8.9%로 나타났으며, 대부분의 발현은 모자이크 형태로 관찰되었다. 따라서 본 연구의 결과에 의하면 소에서 원형질막을 제거한 정자와 외래유전자의 공배양과 이 정자의 난자내 직접도입법에 의해 외래유전자를 가진 형질전환 소 수정란과 형질전환 소 생산이 가능할 것으로 생각된다.

• Advances in Computational Design
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• 제2권2호
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• pp.147-168
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• 2017

Over the past couple decades, externally bonded fiber reinforced polymer (FRP) composites have emerged as a repair and strengthening material for many concrete infrastructure applications. This paper presents an analytical investigation of the use of carbon FRP (CFRP) for a specific problem that occurs in concrete bridge girders wherein the girder ends are damaged by excessive exposure to deicing salts and numerous freezing/thawing cycles. A 3D finite element (FE) model of a full scale prestressed concrete (PC) I-girder is used to investigate the effect of damage to the cover concrete and stirrups in the end region of the girder. Parametric studies are performed using externally bonded CFRP shear laminates to determine the most effective repair schemes for the damaged end region under a short shear span-to-depth ratio. Experimental results on shear pull off tests of CFRP laminates that have undergone accelerated aging are used to calibrate a bond stress-slip model for the interface between the FRP and concrete substrate and approximate the reduced bond stress-slip properties associated with exposure to the environment that causes this type of end region damage. The results of these analyses indicate that this particular application of this material can be effective in recovering the original strength of PC bridge girders with damaged end regions, even after environmental aging.

• 한국건축시공학회지
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• 제5권4호
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• pp.115-120
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• 2005

In recent years, polymer-modified mortars using polymer dispersions have been widely used as finish and repair materials in the construction industry because of their excellent properties compares to those of ordinary cement mortar. Especially, the adhesion improvement of ordinary cement mortar and concrete has attracted a great deal of attention from researchers, and several unique and simply applicable techniques for the adhesion improvement have been developed. The purpose of this study is to evaluate the abrasion resistance of polymer-modified mortar according various curing methods. The polymer-modified mortar are prepared with various polymer-cement ratios, and are subjected to three curing methods such as dry rure, standard cure and freezing and thawing cure after two curing methods, and then tested for abrasion. From the test results, the polymer-modified mortars with various polymer-cement ratios have some superior abrasion resistance compared with plain mortar. The abrasion resistance of polymer-modified mortars increase with an increase in the polymer-cement ratio, and is better under water cure than any other curing methods. It is concluded that the abrasion resistance of cement mortar is markedly improved by modifying of polymer dispersion.