• Mycobiology
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• v.49 no.3
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• pp.258-266
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• 2021

The velvet regulators VosA and VelB are primarily involved in spore maturation and dormancy. Previous studies found that the VosA-VelB hetero-complex coordinates certain target genes that are related to fungal differentiation and conidial maturation in Aspergillus nidulans. Here, we characterized the VosA/VelB-inhibited developmental gene vidD in A. nidulans. Phenotypic analyses demonstrated that the vidD deleted mutant exhibited defect fungal growth, a reduced number of conidia, and delayed formation of sexual fruiting bodies. The deletion of vidD decreased the amount of conidial trehalose, increased the sensitivity against heat stress, and reduced the conidial viability. Moreover, the absence of vidD resulted in increased production of sterigmatocystin. Together, these results show that VidD is required for proper fungal growth, development, and sterigmatocystin production in A. nidulans.

• BMB Reports
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• v.28 no.4
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• pp.368-373
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• 1995

The upstream regulatory region (URR) of bovine papillomavirus type 1 (BPV) contains promoters and a conditional transcriptional enhancer that is trans-activated by the viral E2 protein. After deleting the 5' and 3' ends of BPV URR, BamHI linkers were inserted into several positions of BPV URR without causing an addition or a deletion of URR sequences. Most linker scanning mutations did not show any effects on the transcription of P7940 and P89 promoters in BPV URR. However, several mutants showed reduced transcriptional activities. Based on our results we found that the AP-2 and Sp1 binding sites were important for basal level transcription of BPV URR in the absence of the E2 protein and that the CTF/NF-1 site is dispensable for E2 transactivation of BPV URR transcription.

• Proceedings of the Korean Society for Language and Information Conference
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• 2002.02a
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• pp.36-49
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• 2002

One main complexity of the copula constructions concerns a mismatch between morphology and syntactic constituency: the copula seems to form a morphological unit with the immediately preceding element, whereas in terms of syntax the copula appears to take this as its syntactic complement. In capturing such mismatches, we show that the copula is treated as an independent verb at the level of tectogrammatical structure (or syntax tree), whereas as a bound morpheme at the level of phonogram-matical structure (or domain tree), in terms of Dowty 1992 (or Reape 1994). This paper, adopting the notion of DOMAIN in HPSG, shows that copula constructions are a subtype of compacting-constructions. These constructions compact the domain value of the copula and that of its preceding element together into one domain unit, eventually making it inert to syntactic phenomena such as scrambling, deletion and pro-form substitution. This construction-based approach provides a clean analysis for the formation of the copula construction and related phenomena.

• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1527-1535
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• 2009

Polyphosphate (polyP) plays diverse physiological functions in prokaryotes and eukaryotes, but most of their detailed mechanisms are still obscure. Here, we show that deletion of polyphosphate kinase (PPK), the principal enzyme responsible for synthesis of polyP, resulted in augmented expression of cAMP receptor protein (CRP) and rpoS and lowered $H_2O_2$ sensitivity in Salmonella Typhimurium ATCC14028. The binding of cAMP-CRP complex to rpoS promoter and further stimulation of its transcription were proved through electrophoretic mobility shift assay, lacZ fusion, and exogenous cAMP addition, respectively. The rpoS expression increased in cpdA (cAMP phosphodiesterase coding gene) mutant, further suggesting that cAMP-CRP upregulated rpoS expression. These results demonstrate that PPK affects oxidative stress response by modulating crp and rpoS expression in S. Typhimurium.

• The Korean Journal of Physiology and Pharmacology
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• v.2 no.5
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• pp.645-650
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• 1998

To assess the relationship between angiotensin-converting enzyme (ACE) gene polymorphism and myocardial infarction in Koreans, we recruited 112 healthy, unrelated subjects (mean age 53.4 years) and 104 myocardial infarction survivors (mean age 54.2 years) of both sexes. An insertion/deletion (I/D) polymorphism of the ACE gene was typed by polymerase chain reaction. The I allelic frequency of ACE gene in Korean subjects was irrelavant to myocardial infarction (patients, 65 control subjects 66%), as was true with the D allele. When compared with other populations, the frequency of D allele in Koreans (0.34) was lower than that in Caucasians, and was close to that of other Oriental populations. The data suggest that the ACE gene polymorphism is not an independent genetic risk factor for myocardial infarction in Koreans.

• Korean Journal of English Language and Linguistics
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• v.2 no.4
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• pp.471-494
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• 2002

May(1985) argued that the regress problem of antecedent-contained deletion (ACD) construction could be resolved easily by positing QR. But the proposal has some conceptual and empirical problems. To resolve the problems of QR account on ACD, Hornstein(1994) suggested a new approach viz. LF A-movement approach. In the Minimalist framework, an object raises to the [Spec, Agro] to check the accusative case. Through this movement, the regress problem can be resolved naturally without QR. But the LF A-movement account has the same conceptual problem as the QR approach. In addition to the conceptual problem, it has also empirical problems which were pointed out by Kennedy(1997). To solve the problems, I propose that the object raise to [Spec Agro] overtly as Koizumi(1993, 1995) and Lasnik(1995) insisted. In addition to the proposal, I adopt another assumption that only the copy of a quantifier could become a variable by the vehicle change, which was suggested by Brody(1995). With the above proposals, the unnatural QR operation could be dispensed with and the conceptual and empirical problems which were made concerning the LF A-movement approach could be resolved.

• 한국균학회소식:학술대회논문집
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• 2015.11a
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• pp.23-23
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• 2015

Rice blast disease caused by M. oryzae is the most devastating fungal disease in rice. During the infection process, M. oryzae secretes a large number of glycosyl hydrolase (GH) proteins into the apoplast to digest host cell wall and assist fungal ingress into host tissues. In this study, we identified a novel M. oryze arabinofuranosidase B (MoAbfB) which is secreted during fungal infection. Live-cell imaging exhibited that fluorescent labeled MoAbfB was highly accumulated in fungal invasive structures such as appressorium, tips of penetration peg, biotrophic interfacial complex (BIC), as well as invasive hyphal tip. Deletion of MoAbfB mutants extended biotrophic phase followed by enhanced disease severity, whereas, over-expression of OsMoAbfB mutant induced rapid defense responses and enhanced rice resistance to M. oryzae infection. Furthermore, exogenous treatment of MoAbfB protein showed inhibition of fungal infection via priming of defense gene expression. We later found that the extract of MoAbfB degraded rice cell wall fragments could also induce host defense activation, suggesting that not MoAbfB itself but oligosaccharides (OGs) derived from MoAbfB dissolved rice cell wall elicited rice innate immunity.

• Bulletin of the Korean Chemical Society
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• v.35 no.1
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• pp.83-86
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• 2014

Poly(A) polymerase (PAP) play an essential role for maturation of mRNA by adding the adenylate residues at the 3' end. PAP functions are regulated through protein-protein interaction at its C-terminal region. In this study, cyclophilin A (CypA), a member of the peptidyl-prolyl cis-trans isomerase family, was identified as a partner protein interacting with the C-terminal region PAP. The interaction between PAP and CypA was inhibited by the immunosuppressive drug cyclosporine A. Deletion analysis revealed that the N-terminal 56 residues of CypA are sufficient for the interaction with PAP. Interestingly, we observed that PAP and CypA colocalize in the nucleus during SDF-1-induced chemotaxis, implying that CypA could be involved in the regulation of polyadenylation by PAP in the chemotactic cells.

• Journal of the Korean Data and Information Science Society
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• v.16 no.2
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• pp.429-444
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• 2005

The identification of unusual observations such as outliers and high leverage points has drawn a great deal of attention for many years. Most of these identifications techniques are based on case deletion that focuses more on the outliers than the high leverage points. But residuals together with leverage values may cause masking and swamping for which a good number of unusual observations remain undetected in the presence of multiple outliers and multiple high leverage points. In this paper we propose a new procedure to identify outliers and high leverage points simultaneously. We suggest an additive form of the residuals and the leverages that gives almost an equal focus on outliers and leverages. We analyzed several well-referred data set and discover few outliers and high leverage points that were undetected by the existing diagnostic techniques.

• English Language & Literature Teaching
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• v.10 no.1
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• pp.179-200
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• 2004

The purpose of this study is to investigate the adequacy of derivational approach and representational approach to syntactic theories in generative grammar. As the generative grammar is based on the derivational process of syntactic theories, it is suggested that derivational approach is more valid than representational approach. Move, Economy Principle, Local Economy and Label-free Phrase Structure in Minimalist framework support the preference of derivational approach to representational approach with the elimination of computational complexity, minimality, and label-free phrase structure. Syntactic structure is considered as the result of the interaction of the properties of lexical items containing probe and economy conditions constrained by bare output conditions. On the other hand, Pseudogapping in Lasnik(1999) is analyzed in terms of Object Shift, that is, overt raising to Spec of $Agr_o$ and the PF deletion of VP in representational approach. Hence, it is suggested that the combination of derivational and representational approaches to syntactic theories can be admitted in generative grammar.