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Characterization of a Glutamate Decarboxylase (GAD) from Enterococcus avium M5 Isolated from Jeotgal, a Korean Fermented Seafood

  • Lee, Kang Wook;Shim, Jae Min;Yao, Zhuang;Kim, Jeong A;Kim, Hyun-Jin;Kim, Jeong Hwan
    • Journal of Microbiology and Biotechnology
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    • v.27 no.7
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    • pp.1216-1222
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    • 2017
  • To develop starters for the production of functional foods or materials, lactic acid bacteria producing ${\gamma}-aminobutyric$ acid (GABA) were screened from jeotgals, Korean fermented seafoods. One isolate producing a high amount of GABA from monosodium $\text\tiny{L}$-glutamate (MSG) was identified as Enterococcus avium by 16S rRNA gene sequencing. E. avium M5 produced $18.47{\pm}1.26mg/ml$ GABA when incubated for 48 h at $37^{\circ}C$ in MRS broth with MSG (3% (w/v)). A gadB gene encoding glutamate decarboxylase (GAD) was cloned and overexpressed in E. coli BL21 (DE3) using the pET26b (+) expression vector. Recombinant GAD was purified through a Ni-NTA column and the size was estimated to be 53 kDa by SDS-PAGE. Maximum GAD activity was observed at pH 4.5 and $55^{\circ}C$and the activity was dependent on pyridoxal 5'-phosphate. The $K_m$ and $V_{max}$ values of GAD were $3.26{\pm}0.21mM$ and $0.0120{\pm}0.0001mM/min$, respectively, when MSG was used as a substrate. Enterococcus avium M5 secretes a lot of GABA when grown on MRS with MSG, and the strain is useful for the production of fermented foods containing a high amount of GABA.

Improved Bag of Visual Words Image Classification Using the Process of Feature, Color and Texture Information (특징, 색상 및 텍스처 정보의 가공을 이용한 Bag of Visual Words 이미지 자동 분류)

  • Park, Chan-hyeok;Kwon, Hyuk-shin;Kang, Seok-hoon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2015.10a
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    • pp.79-82
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    • 2015
  • Bag of visual words(BoVW) is one of the image classification and retrieval methods, using feature point that automatical sorting and searching system by image feature vector of data base. The existing method using feature point shall search or classify the image that user unwanted. To solve this weakness, when comprise the words, include not only feature point but color information that express overall mood of image or texture information that express repeated pattern. It makes various searching possible. At the test, you could see the result compared between classified image using the words that have only feature point and another image that added color and texture information. New method leads to accuracy of 80~90%.

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Detection of Depression Trends in Literary Cyber Writers Using Sentiment Analysis and Machine Learning

  • Faiza Nasir;Haseeb Ahmad;CM Nadeem Faisal;Qaisar Abbas;Mubarak Albathan;Ayyaz Hussain
    • International Journal of Computer Science & Network Security
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    • v.23 no.3
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    • pp.67-80
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    • 2023
  • Rice is an important food crop for most of the population in Nowadays, psychologists consider social media an important tool to examine mental disorders. Among these disorders, depression is one of the most common yet least cured disease Since abundant of writers having extensive followers express their feelings on social media and depression is significantly increasing, thus, exploring the literary text shared on social media may provide multidimensional features of depressive behaviors: (1) Background: Several studies observed that depressive data contains certain language styles and self-expressing pronouns, but current study provides the evidence that posts appearing with self-expressing pronouns and depressive language styles contain high emotional temperatures. Therefore, the main objective of this study is to examine the literary cyber writers' posts for discovering the symptomatic signs of depression. For this purpose, our research emphases on extracting the data from writers' public social media pages, blogs, and communities; (3) Results: To examine the emotional temperatures and sentences usage between depressive and not depressive groups, we employed the SentiStrength algorithm as a psycholinguistic method, TF-IDF and N-Gram for ranked phrases extraction, and Latent Dirichlet Allocation for topic modelling of the extracted phrases. The results unearth the strong connection between depression and negative emotional temperatures in writer's posts. Moreover, we used Naïve Bayes, Support Vector Machines, Random Forest, and Decision Tree algorithms to validate the classification of depressive and not depressive in terms of sentences, phrases and topics. The results reveal that comparing with others, Support Vectors Machines algorithm validates the classification while attaining highest 79% f-score; (4) Conclusions: Experimental results show that the proposed system outperformed for detection of depression trends in literary cyber writers using sentiment analysis.

Automated Prioritization of Construction Project Requirements using Machine Learning and Fuzzy Logic System

  • Hassan, Fahad ul;Le, Tuyen;Le, Chau;Shrestha, K. Joseph
    • International conference on construction engineering and project management
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    • 2022.06a
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    • pp.304-311
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    • 2022
  • Construction inspection is a crucial stage that ensures that all contractual requirements of a construction project are verified. The construction inspection capabilities among state highway agencies have been greatly affected due to budget reduction. As a result, efficient inspection practices such as risk-based inspection are required to optimize the use of limited resources without compromising inspection quality. Automated prioritization of textual requirements according to their criticality would be extremely helpful since contractual requirements are typically presented in an unstructured natural language in voluminous text documents. The current study introduces a novel model for predicting the risk level of requirements using machine learning (ML) algorithms. The ML algorithms tested in this study included naïve Bayes, support vector machines, logistic regression, and random forest. The training data includes sequences of requirement texts which were labeled with risk levels (such as very low, low, medium, high, very high) using the fuzzy logic systems. The fuzzy model treats the three risk factors (severity, probability, detectability) as fuzzy input variables, and implements the fuzzy inference rules to determine the labels of requirements. The performance of the model was examined on labeled dataset created by fuzzy inference rules and three different membership functions. The developed requirement risk prediction model yielded a precision, recall, and f-score of 78.18%, 77.75%, and 75.82%, respectively. The proposed model is expected to provide construction inspectors with a means for the automated prioritization of voluminous requirements by their importance, thus help to maximize the effectiveness of inspection activities under resource constraints.

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Multi-Emotion Regression Model for Recognizing Inherent Emotions in Speech Data (음성 데이터의 내재된 감정인식을 위한 다중 감정 회귀 모델)

  • Moung Ho Yi;Myung Jin Lim;Ju Hyun Shin
    • Smart Media Journal
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    • v.12 no.9
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    • pp.81-88
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    • 2023
  • Recently, communication through online is increasing due to the spread of non-face-to-face services due to COVID-19. In non-face-to-face situations, the other person's opinions and emotions are recognized through modalities such as text, speech, and images. Currently, research on multimodal emotion recognition that combines various modalities is actively underway. Among them, emotion recognition using speech data is attracting attention as a means of understanding emotions through sound and language information, but most of the time, emotions are recognized using a single speech feature value. However, because a variety of emotions exist in a complex manner in a conversation, a method for recognizing multiple emotions is needed. Therefore, in this paper, we propose a multi-emotion regression model that extracts feature vectors after preprocessing speech data to recognize complex, inherent emotions and takes into account the passage of time.

A Unicode based Deep Handwritten Character Recognition model for Telugu to English Language Translation

  • BV Subba Rao;J. Nageswara Rao;Bandi Vamsi;Venkata Nagaraju Thatha;Katta Subba Rao
    • International Journal of Computer Science & Network Security
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    • v.24 no.2
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    • pp.101-112
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    • 2024
  • Telugu language is considered as fourth most used language in India especially in the regions of Andhra Pradesh, Telangana, Karnataka etc. In international recognized countries also, Telugu is widely growing spoken language. This language comprises of different dependent and independent vowels, consonants and digits. In this aspect, the enhancement of Telugu Handwritten Character Recognition (HCR) has not been propagated. HCR is a neural network technique of converting a documented image to edited text one which can be used for many other applications. This reduces time and effort without starting over from the beginning every time. In this work, a Unicode based Handwritten Character Recognition(U-HCR) is developed for translating the handwritten Telugu characters into English language. With the use of Centre of Gravity (CG) in our model we can easily divide a compound character into individual character with the help of Unicode values. For training this model, we have used both online and offline Telugu character datasets. To extract the features in the scanned image we used convolutional neural network along with Machine Learning classifiers like Random Forest and Support Vector Machine. Stochastic Gradient Descent (SGD), Root Mean Square Propagation (RMS-P) and Adaptative Moment Estimation (ADAM)optimizers are used in this work to enhance the performance of U-HCR and to reduce the loss function value. This loss value reduction can be possible with optimizers by using CNN. In both online and offline datasets, proposed model showed promising results by maintaining the accuracies with 90.28% for SGD, 96.97% for RMS-P and 93.57% for ADAM respectively.

Customer Behavior Prediction of Binary Classification Model Using Unstructured Information and Convolution Neural Network: The Case of Online Storefront (비정형 정보와 CNN 기법을 활용한 이진 분류 모델의 고객 행태 예측: 전자상거래 사례를 중심으로)

  • Kim, Seungsoo;Kim, Jongwoo
    • Journal of Intelligence and Information Systems
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    • v.24 no.2
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    • pp.221-241
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    • 2018
  • Deep learning is getting attention recently. The deep learning technique which had been applied in competitions of the International Conference on Image Recognition Technology(ILSVR) and AlphaGo is Convolution Neural Network(CNN). CNN is characterized in that the input image is divided into small sections to recognize the partial features and combine them to recognize as a whole. Deep learning technologies are expected to bring a lot of changes in our lives, but until now, its applications have been limited to image recognition and natural language processing. The use of deep learning techniques for business problems is still an early research stage. If their performance is proved, they can be applied to traditional business problems such as future marketing response prediction, fraud transaction detection, bankruptcy prediction, and so on. So, it is a very meaningful experiment to diagnose the possibility of solving business problems using deep learning technologies based on the case of online shopping companies which have big data, are relatively easy to identify customer behavior and has high utilization values. Especially, in online shopping companies, the competition environment is rapidly changing and becoming more intense. Therefore, analysis of customer behavior for maximizing profit is becoming more and more important for online shopping companies. In this study, we propose 'CNN model of Heterogeneous Information Integration' using CNN as a way to improve the predictive power of customer behavior in online shopping enterprises. In order to propose a model that optimizes the performance, which is a model that learns from the convolution neural network of the multi-layer perceptron structure by combining structured and unstructured information, this model uses 'heterogeneous information integration', 'unstructured information vector conversion', 'multi-layer perceptron design', and evaluate the performance of each architecture, and confirm the proposed model based on the results. In addition, the target variables for predicting customer behavior are defined as six binary classification problems: re-purchaser, churn, frequent shopper, frequent refund shopper, high amount shopper, high discount shopper. In order to verify the usefulness of the proposed model, we conducted experiments using actual data of domestic specific online shopping company. This experiment uses actual transactions, customers, and VOC data of specific online shopping company in Korea. Data extraction criteria are defined for 47,947 customers who registered at least one VOC in January 2011 (1 month). The customer profiles of these customers, as well as a total of 19 months of trading data from September 2010 to March 2012, and VOCs posted for a month are used. The experiment of this study is divided into two stages. In the first step, we evaluate three architectures that affect the performance of the proposed model and select optimal parameters. We evaluate the performance with the proposed model. Experimental results show that the proposed model, which combines both structured and unstructured information, is superior compared to NBC(Naïve Bayes classification), SVM(Support vector machine), and ANN(Artificial neural network). Therefore, it is significant that the use of unstructured information contributes to predict customer behavior, and that CNN can be applied to solve business problems as well as image recognition and natural language processing problems. It can be confirmed through experiments that CNN is more effective in understanding and interpreting the meaning of context in text VOC data. And it is significant that the empirical research based on the actual data of the e-commerce company can extract very meaningful information from the VOC data written in the text format directly by the customer in the prediction of the customer behavior. Finally, through various experiments, it is possible to say that the proposed model provides useful information for the future research related to the parameter selection and its performance.

Increasing Accuracy of Classifying Useful Reviews by Removing Neutral Terms (중립도 기반 선택적 단어 제거를 통한 유용 리뷰 분류 정확도 향상 방안)

  • Lee, Minsik;Lee, Hong Joo
    • Journal of Intelligence and Information Systems
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    • v.22 no.3
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    • pp.129-142
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    • 2016
  • Customer product reviews have become one of the important factors for purchase decision makings. Customers believe that reviews written by others who have already had an experience with the product offer more reliable information than that provided by sellers. However, there are too many products and reviews, the advantage of e-commerce can be overwhelmed by increasing search costs. Reading all of the reviews to find out the pros and cons of a certain product can be exhausting. To help users find the most useful information about products without much difficulty, e-commerce companies try to provide various ways for customers to write and rate product reviews. To assist potential customers, online stores have devised various ways to provide useful customer reviews. Different methods have been developed to classify and recommend useful reviews to customers, primarily using feedback provided by customers about the helpfulness of reviews. Most shopping websites provide customer reviews and offer the following information: the average preference of a product, the number of customers who have participated in preference voting, and preference distribution. Most information on the helpfulness of product reviews is collected through a voting system. Amazon.com asks customers whether a review on a certain product is helpful, and it places the most helpful favorable and the most helpful critical review at the top of the list of product reviews. Some companies also predict the usefulness of a review based on certain attributes including length, author(s), and the words used, publishing only reviews that are likely to be useful. Text mining approaches have been used for classifying useful reviews in advance. To apply a text mining approach based on all reviews for a product, we need to build a term-document matrix. We have to extract all words from reviews and build a matrix with the number of occurrences of a term in a review. Since there are many reviews, the size of term-document matrix is so large. It caused difficulties to apply text mining algorithms with the large term-document matrix. Thus, researchers need to delete some terms in terms of sparsity since sparse words have little effects on classifications or predictions. The purpose of this study is to suggest a better way of building term-document matrix by deleting useless terms for review classification. In this study, we propose neutrality index to select words to be deleted. Many words still appear in both classifications - useful and not useful - and these words have little or negative effects on classification performances. Thus, we defined these words as neutral terms and deleted neutral terms which are appeared in both classifications similarly. After deleting sparse words, we selected words to be deleted in terms of neutrality. We tested our approach with Amazon.com's review data from five different product categories: Cellphones & Accessories, Movies & TV program, Automotive, CDs & Vinyl, Clothing, Shoes & Jewelry. We used reviews which got greater than four votes by users and 60% of the ratio of useful votes among total votes is the threshold to classify useful and not-useful reviews. We randomly selected 1,500 useful reviews and 1,500 not-useful reviews for each product category. And then we applied Information Gain and Support Vector Machine algorithms to classify the reviews and compared the classification performances in terms of precision, recall, and F-measure. Though the performances vary according to product categories and data sets, deleting terms with sparsity and neutrality showed the best performances in terms of F-measure for the two classification algorithms. However, deleting terms with sparsity only showed the best performances in terms of Recall for Information Gain and using all terms showed the best performances in terms of precision for SVM. Thus, it needs to be careful for selecting term deleting methods and classification algorithms based on data sets.

Research on hybrid music recommendation system using metadata of music tracks and playlists (음악과 플레이리스트의 메타데이터를 활용한 하이브리드 음악 추천 시스템에 관한 연구)

  • Hyun Tae Lee;Gyoo Gun Lim
    • Journal of Intelligence and Information Systems
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    • v.29 no.3
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    • pp.145-165
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    • 2023
  • Recommendation system plays a significant role on relieving difficulties of selecting information among rapidly increasing amount of information caused by the development of the Internet and on efficiently displaying information that fits individual personal interest. In particular, without the help of recommendation system, E-commerce and OTT companies cannot overcome the long-tail phenomenon, a phenomenon in which only popular products are consumed, as the number of products and contents are rapidly increasing. Therefore, the research on recommendation systems is being actively conducted to overcome the phenomenon and to provide information or contents that are aligned with users' individual interests, in order to induce customers to consume various products or contents. Usually, collaborative filtering which utilizes users' historical behavioral data shows better performance than contents-based filtering which utilizes users' preferred contents. However, collaborative filtering can suffer from cold-start problem which occurs when there is lack of users' historical behavioral data. In this paper, hybrid music recommendation system, which can solve cold-start problem, is proposed based on the playlist data of Melon music streaming service that is given by Kakao Arena for music playlist continuation competition. The goal of this research is to use music tracks, that are included in the playlists, and metadata of music tracks and playlists in order to predict other music tracks when the half or whole of the tracks are masked. Therefore, two different recommendation procedures were conducted depending on the two different situations. When music tracks are included in the playlist, LightFM is used in order to utilize the music track list of the playlists and metadata of each music tracks. Then, the result of Item2Vec model, which uses vector embeddings of music tracks, tags and titles for recommendation, is combined with the result of LightFM model to create final recommendation list. When there are no music tracks available in the playlists but only playlists' tags and titles are available, recommendation was made by finding similar playlists based on playlists vectors which was made by the aggregation of FastText pre-trained embedding vectors of tags and titles of each playlists. As a result, not only cold-start problem can be resolved, but also achieved better performance than ALS, BPR and Item2Vec by using the metadata of both music tracks and playlists. In addition, it was found that the LightFM model, which uses only artist information as an item feature, shows the best performance compared to other LightFM models which use other item features of music tracks.

Utilizing Unlabeled Documents in Automatic Classification with Inter-document Similarities (문헌간 유사도를 이용한 자동분류에서 미분류 문헌의 활용에 관한 연구)

  • Kim, Pan-Jun;Lee, Jae-Yun
    • Journal of the Korean Society for information Management
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    • v.24 no.1 s.63
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    • pp.251-271
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    • 2007
  • This paper studies the problem of classifying documents with labeled and unlabeled learning data, especially with regards to using document similarity features. The problem of using unlabeled data is practically important because in many information systems obtaining training labels is expensive, while large quantities of unlabeled documents are readily available. There are two steps In general semi-supervised learning algorithm. First, it trains a classifier using the available labeled documents, and classifies the unlabeled documents. Then, it trains a new classifier using all the training documents which were labeled either manually or automatically. We suggested two types of semi-supervised learning algorithm with regards to using document similarity features. The one is one step semi-supervised learning which is using unlabeled documents only to generate document similarity features. And the other is two step semi-supervised learning which is using unlabeled documents as learning examples as well as similarity features. Experimental results, obtained using support vector machines and naive Bayes classifier, show that we can get improved performance with small labeled and large unlabeled documents then the performance of supervised learning which uses labeled-only data. When considering the efficiency of a classifier system, the one step semi-supervised learning algorithm which is suggested in this study could be a good solution for improving classification performance with unlabeled documents.