• 한국자원식물학회지
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• 제20권6호
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• pp.558-562
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• 2007

The high-performance liquid chromatography(HPLC) method for the determination of trans-resveratrol in 34 germplasms and processing methods of peanut seeds has been modified. Peanut germplasms contained trans-resveratrol contents of $0.14{\sim}4.96{\mu}g/g$, but findings for the testa color were not significant. However, two germplasms, 'KIGAN' and 'CS1', contained more trans-resveratrol contents than the other germplasms. The contents of their were $2.26{\mu}g/g\;and\;4.96{\mu}g/g$. The tested processing methods caused no significant changes in trans-resveratrol contents. The contents of fresh, boiled, and roasted peanuts were 0.36, 0.32, and $0.40{\mu}g/g$, respectively in cv. Palkwang, and 0.22, 0.22, and $0.26{\mu}g/g$, respectively, in cv. Jakwang. Differences were not significant among fresh, boiled, and roasted peanuts. The grains of 'Palkwang' and 'Jakwang' contained trans-resveratrol contents of $0.34{\mu}g/g\;and\;0.24{\mu}g/g$, and testa contained $1.12{\mu}g/g\;and\;1.00{\mu}g/g$, respectively. However, when comparing absolute quantity, the trans-resveratrol contents appears to be approximately $3{\sim}4$ times higher in the testa than in the grain of the peanut, although the total contents were not different because the ratio of testa was low in peanut seeds.

• Journal of Plant Biotechnology
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• 제42권2호
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• pp.123-128
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• 2015

Pregermination treatment of cocoa beans either with the testa, group PCB (+T), or without the testa, group PCB (-T), was studied here to determine whether this treatment (incubation up to 120 h at $25^{\circ}C$) has any effect on the levels of allergenic proteins or on chemical composition. Our proximate analysis included carbohydrates, proteins, and lipids. We used western blotting to measure changes in the amounts of allergenic proteins in the cocoa beans during the pregermination treatment. The protein and carbohydrate content of both groups (with or without the testa) decreased slightly during this treatment, whereas lipid content increased. Group PCB (-T) showed more rapid metabolic processes than did group PCB (+T) during the pregermination treatment. Western blot analysis showed that the cocoa beans contained an allergenic protein of ~28 kDa. Removal of the testa strongly reduced the amount of this allergenic protein after 72 h of the pregermination treatment. Generally, the pregermination treatment increased antioxidant activity in both groups. Significant differences in the antioxidant activity were observed between groups PCB (-T) and PCB (+T). Particularly, group PCB (-T) showed high antioxidant activity at 72 h of the pregermination treatment. Thus, the combination of cocoa beans without the testa and pregermination treatment (72 h) seems to be the optimal method for production of hypoallergenic cocoa beans rich in antioxidants for patients with allergic disorders.

• Natural Product Sciences
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• 제15권2호
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• pp.96-100
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• 2009

A novel gastric ulcer healing formulation, a mixture of Dioscoreae Rhizoma, Halloysitum Rubrum and Ostreae Testa (Dihaos), was examined for gastric ulcer healing effects. The effect of Dihaos was assessed in various gastric ulcer models in rats. Oral administration of Dihaos significantly reduced HCl-ethanol-induced gastric ulcers. Dihaos also significantly reduced gastric and duodenal ulcers induced by cysteamine. Ostreae Testa decreased secretion of gastric juice and increased the pH of gastric juice. Furthermore, the extracts of Dioscoreae Rhizoma affected the cell proliferation of MKN 74 cells. These results suggest that the healing effect of Dihaos on gastrohemorrhagic lesions results from its protective effect against acid secretion and proliferation of mucosal cells in induced gastric ulcers in rats.

• Applied Microscopy
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• 제42권4호
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• pp.194-199
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• 2012

The anatomy and morphology of seeds from 10 Hawaiian Portulaca taxa were examined to explore patterns of variation among the taxa, and to evaluate their phylogenetic relationships. Features of seeds were assessed employing microtechnique procedures, statistical analysis, and scanning electron microscopy (SEM). Anatomically, the orientation of embryo was consistent across taxa, and all seeds examined had central nutritive tissue and integuments comprising the peripheral embryo. Seeds were generally small, circular to ovoid in shape, and either smooth or rough-surfaced, with tubercules. Variation in seed size was noted, although minimal within small seeded group. The size of seeds ranged from 0.50~1.26 mm in length, and 0.55~1.34 mm in width, with similar weights. Seeds were distinguished by the sculpture and arrangement of the testa epidermis and the way in which their stellulate-tessellate cells uniquely fit together. Using a multiple range test (ANOVA), two groups were established by seed characteristics. The SEM also demonstrated morphological differences in testa sculpturing. The results obtained confirm the usefulness of seed characteristics in the identification of the species examined, and furthermore, allow for the separation of the Hawaiian Portulaca into two groups.

• 식물분류학회지
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• 제38권4호
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• pp.389-403
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• 2008

대극과 대극속 Zygophyllidium절의 한계와 종간 유연관계를 알아보기 위하여 Zygophyllidium절과 Poinsettia아속에 속하는 12종 종자를 광학현미경과 주사전자현미경으로 관찰하였다. 12종에 대해 종자의 특징을 기재하였으며, 검색표를 작성하였다. 9개의 종자 형질을 이용한 수리분석 결과를 바탕으로 다음과 같이 6개의 유형으로 구분하였다. Type I(Euphorbia uniglandulosa)는 종자표면에 작은 구멍이 있으며, 세포표면에 미세입자가 존재한다. Type II(E. exstipulata, E. lagunensis, E. bilobata, E. hexagonoides, E. chersonesa)는 종자 표면의 testa cell 사이에 미세과립을 가진다. Type III(E. cyathophora)는 종자의 선단이 뾰족, 세포 표면에는 동심원의 주름을 가진다. Type IV(E. dentata와 E. pentadactyla)는 종부를 갖고 있으며 testa cell 사이에 미세과립이 없다. Type V(E. hexagona)는 둥근 난형으로 세포 간 미세과립이 존재하지 않는다. Type VI(E. eriantha, E. lacera)는 종자의 모양이 사각형이며, 종부를 가지고 있다. 본 연구를 통해 Zygophyllidium절을 E. exstipulata, E. lagunensis, E. bilobata, E. hexagonoides, E. chersonesa만을 포함하는 Type II의 분류군들로 설정하는 것을 제안하며, 전통적으로 Zygophyllidium절에 포함시켰던 E. hexagona는 Poinsettia아속의 E. dentata와 유연관계가 있음을 시사하고 있다.

• 한국작물학회지
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• 제52권3호
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• pp.239-248
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• 2007

현재 판매되고 있는 브랜드콩 100개(장류용 콩 32개, 검정콩 45개, 약콩 17개 및 나물용 콩 6개)를 2006년에 전국의 중대형마트에서 수집하고, 대비하기 위하여 재래시장에서 판매되는 콩 10개를 수집하여 표시의 정확성과 외관 품질을 분석한 결과는 다음과 같다. 1. 브랜드콩의 명칭은 영양성, 친환경 또는 친근감을 느끼는 용어와 함께 장류용 콩은 백태와 황태, 검정콩은 검정콩, 서리태, 늦서리태, 흑태, 청태, 속청, 속태, 그리고 약콩과 나물용 콩 등으로 다양하게 표기하였다. 2. 시판되는 콩의 판매단위는 1 kg 포장이 30%이고, 500 g 포장이 59%였으며, 최소포장단위는 200 g이었다. 표시중량과 실중량의 편차는 70%가 ${\pm}1%$였고, 약 2/3는 실중량이 표시중량 이상으로 비교적 정확하였다. 3. 상품에 무농약 농산물로 표시된 것이 6개였고, 특품 또는 상품으로 표시된 것이 9개였으나 외관 품질과는 관계가 적었다. 4. 상품화되어 판매되는 콩의 100립중 범위는 장류용 콩이 $23.7{\sim}47.8g$, 검정콩은 $21.9{\sim}44.5g$ 그리고 약콩과 나물용 콩은 $9.5{\sim}15.0g$이었다. 장류용 콩과 검정콩은 100 립중 범위가 비슷하였으나 장류용 콩은 29 g 이하가 63%인데 비하여 검정콩은 78%가 30 g이상으로 검정콩이 대체적으로 굵었다. 5. 장류용 콩과 검정콩을 크기 6.70 mm 체로 선별하였을 때 남는 콩의 비율은 평균과 최대치가 각각 87.4%와 86.5% 및 99.9%와 99.5%로 비슷하였으나 최소치는 각각 70.7%와 14.4%로 차이가 컸다. 콩의 100립중과 6.7mm 체로 선별하여 남은 콩의 비율은 유의한 정의 상관(각각 0.7488**과 0.7874**)이 있었다. 6. 종실의 100립중이 30 g 이상인 것들은 선별 비율이 90% 이상으로 차이가 작고 종실의 크기가 균일하였으나, 30 g 이하인 것들은 100립중이 낮을수록 선별되는 비율이 낮아 균일도가 떨어졌다. 7 제색(臍色)과 종실의 형태에 의하여 종실의 혼입여부를 조사한 결과 수집 브랜드콩의 76%는 10% 이상 서로 다른 종실들이 혼합되었는데, 장류용 콩은 90% 이상 혼입되어 검정콩 또는 약콩과 나물용 콩에 비하여 혼입율이 높았다. 8. 상품의 20%에 돌, 포대조각, 나무 조각, 곤충의 유충, 균사, 이종 콩 종자 등과 같은 이물질이 섞여 있어 상품의 품질을 저하시키는 원인이 되었다. 9. 브랜드콩의 용적중은 762 g $L^{-1}$였고, $645{\sim}820g\;L^{-1}$의 범위로 변이가 컸으며, 장류용 콩이 $747g\;L^{-1}$로 가장 낮았고, 약콩이 $758g\;L^{-1}$로 가장 높았다. 10. 재래시장에서 구입한 콩과 브랜드콩은 100립중, 정립율, 이물질 함량, 용적중 등 품질 평가 기준에서 차이가 없었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.64-64
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• 2019

In order to evaluate the taxonomical relevance of seeds morphological characteristics, a macroand micro-morphological study was conducted on seeds of Korean Vicia (Fabacea). We collected 19 taxa of genus Vicia distributed in Korea and introduced one taxa from USDA. The morphological characteristic and testa texture of seeds were investigated using a Stereo-microscope (SM) and Scanning Electron Microscope (SEM). Most of Vicia seeds were found spherical or oblong and some seeds were oval and subglose. The largest seed was V. chosenensis ($4.3{\times}3.6{\times}2.6mm$), and the smallest was V. teterasperma ($1.7{\times}1.7{\times}1.5mm$). V. chosenensis and V. hirsuta were separated from other Vicia species by having a shiny in seed finish. In hilum shape, 14 species have linear and V. sepium was distinguished by having a circumlinear. In testa texture, they developed papilae, only V. hirsuta has lophate in level type. Deposition of the sheet-like debris between the papilae was observed in V. chosenensis, V. cracca, and V. unijuga. Polymorphism information content (PIC) values of the 13 qualitative morphological characters (QMC) were in the range of 0.0950 to 8863 with an average of 0.4611. PIC value of seed shape, seed colour, hilum colour were 0.7403, 0.8177, 0.883 respectively. Cluster analysis based on QMC detected three main clades. V. cracca, V. amurensis, V. amoena were involved in Group 1 and V. unijuga f. minor, V. unijuga, V. unijuga f. angustifolia, V. sepium, V. hirticalycina, V. hirsuta, V. linearfolia, V. chosenensis, V. pseudorobus, V. venosa var. cuspidata were involved in Group 2. V. nipponica, V. japonica, V. villosa, V. dasicarpa, V. bungei, V. angustifolia, V. tetrasperma were clustered in Group 3. Our research suggests that morphological characteristic and testa texture of seeds could be used as definers for the identification of genus Vicia.

• 한국광과학회:학술대회논문집
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• 한국광과학회 1999년도 학술대회지
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• pp.79-80
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• 1999

• 생약학회지
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• 제4권1호
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• pp.9-17
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• 1973

A score and more kinds of molluscan shells have been used as drugs in oriental medicine. The Korean Pharmacopoeia (K.P.) contains a monograph on oyster shell "Ostreae Testa" as an official drug. A huge deposit of the fossil shell Crassostrea gravitesta eoilensis has been recently excavated in the region of Wolsung, Kyong-Sang-Buk-Do. This study was conducted to determine the applicability of the fossil shell as a substiute for Ostreae Testa, K.P. The fossil shell not only met the K.P. requirements, but also exhibited an appropriate antacid activity. Amino acid survivals were also determined quantitatively with amino acid autoanalyzer.

• 한국약용작물학회지
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• 제4권2호
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• pp.172-177
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• 1996

개상사화의 종자발아촉진(種子發芽促進)을 위하여 종피제거(種皮除去), 배유(胚乳)의 일부절제(切除)와 xylene처리(處理), 생장조정제(生長調整劑)와 화학약품처리(化學藥品處理) 및 저온처리(低溫處理)를 실시(實施)하여 파종 4개월후 발아 결과(結果)는 다음과 같다. 1. 종피제거(種皮除去)는 무제거(無除去)에 비해 발아율(發芽率)을 높일 수 있었고 특히 종피제거(種皮除去)+xylene처리(處理) 는 63%의 발아율을 얻었다. 2. $GA_3$는 kinetin 처리(處理)보다 효과적(效果的)이었고 특히 $GA_3$ 200ppm에서 60%의 발아율(發芽率)을 나타내었다. 3. $KNO_3$는 KOH 처리(處理)보다 효과적(效果的)이었으며 특히 $KNO_3$는 0. 1% 처리(處理)에서 76%의 높은 발아율(發芽率)을 나타내었다. 4. 종자(種子)를 $5^{\circ}C$ 1개월(個月) 저온처리후(低溫處理後) 파종(播種)한 발아율(發芽率)은 77%, 2개월(個月) 처리(處理)는 93%, 3개월(個月) 83%, 4개월(個月) 80%로 $5^{\circ}C$ 저온(低溫) 2개월(個月) 처리후(處理後) 파종(播種)에서 가장 높은 발아율(發芽率)을 나타내었다.