• 대한기계학회논문집A
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• 제26권11호
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• pp.2429-2435
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• 2002

The aim of this paper is to investigate the problem of strip down method, which is usually used to evaluate the engine friction level. The validity of strip down method was investigated by theoretical analysis of friction in crank and piston assembly. The friction of cylinder and piston assembly was analyzed under the various test conditions. The measured cylinder pressure was used as boundary conditions of friction torque and loss calculation. The friction loss of crank and piston assembly was influenced by test conditions that resulted from the variation of load condition. From the results, we have known that the strip down method could be possible to distort the friction loss of engine moving components.

• 한국콘텐츠학회논문지
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• 제14권3호
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• pp.52-58
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• 2014

현장검사는 환자가 있는 즉석에서 검사하여 결과를 알 수 있으며 신속하고 경제적으로 시행할 수 있는 장점이 있어 널리 이용되고 있다. 특히 면역크로마토그래피법을 이용한 검사 항목은 편리하게 쓰이고 있으나 오류가 발생하여 재확인 할 경우, 판독선이 시간이 경과됨에 따라 재확인이 불가능한 단점이 있고 반응이 약한 경우 판독자에 따라 오류가 생길 수 있다. 이에 검사에 쓰이는 다양한 현장검사용 스트립의 판독이 가능하고 화상을 저장할 수 있는 기능을 가진 멀티 스트립 리더기를 개발하였고, 요 임신검사가 의뢰된 검체를 대상으로 개발된 멀티 스트립 리더기를 평가한 결과 100% 일치된 결과를 보였다. 본 연구에서 개발된 멀티 스트립 리더기는 실제 임상에서 편리하고 경제적으로 사용될 수 있을 것으로 생각된다.

• 소성∙가공
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• 제10권1호
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• pp.53-58
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• 2001

Set-up reference values, used in determining the optimum downcoiling tension, we experimentally verified in this study. During the actual downcoiling, the strip suffers both tension and bending force through the rotation of mandrel. Therefore, simulative test which can measure both tension and bending resistance of strip was performed to estimate set-up reference value for strip tension during downcoiling operations. The values obtained from the simulative test were correlated with the yield stress which has conventionally been used as reference values for downcoiling tension. The correlative analysis showed that the yield stress of strip can be a good reference value for downcoiling tension. Furthermore, the bending load also shows strong correlation with simulated values due to the close relationship between yield stress and bending load.

• 대한기계학회논문집A
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• 제34권9호
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• pp.1299-1304
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• 2010

압연실험을 통해서 폭 방향 변형 편차가 존재하는 실리콘 강판의 가공파손을 고찰하였다. 폭 방향 변형 편차는 강판에 웨이브를 발생시키고 압연방향으로 소재의 에지 부위(혹은 센터 부위)에 인장(혹은 압축)을 준다. 실제 압연기에서 발생하는 웨이브를 실험실적으로 구현하기 위해서 공형롤을 설계 및 제작하였다. 실험에 사용한 소재는 고-실리콘(약 3%) 강판이다. 본 실험을 통해서 센터 웨이브에 의해 발생되는 에지 부위 인장응력이 가공파손에 가장 지배적인 요인이라는 것이 제시되었다. 센터 웨이브를 일으키는 폭 방향 변형 편차의 정도에 따라 에지 파손과 지그재그 형태의 시편 중심 절손을 유발한다는 결과도 도출하였다.

• Archives of Pharmacal Research
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• 제16권3호
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• pp.227-230
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• 1993

A new device to detect methamphetamine (MA), amphetamine(A) and its metabolites in urine was developed using the paper strip method and the test tube method of dry chemical reagents. The reagent containing tetrabromophenolphthalein ethyl ester (TBPE) and borax. For the TBPE paper strip method, a device was prepared with a window at each end of the reagent paper strip ; one window is for the sample application, and the other window is for the methylene chloride. The diffused sample from one window reacts with reagent in the paper and produces color at the point where it meets with methylene chloride which has diffused form the other side. A positive smaple produces as red-purple color and the negative sample a greenish color, with a detection limit of 5-10 ppm. The result can be obtained within one minute. For the TBPE test tube method which contains dry reagents, the detection limit is 5 ppm and the result can be obtaineed within 30 seconds, however the carry-on is not as convenient as the paper strip method. The performance of both methods were evlauated by comparing with the results of gas chromatography (GC) and fluorescence polarizaiton immunoassay (FPIA). The results were proven that both methods were useful as primary screening reagents to detect MA in urine and in dry powder.

• 농업과학연구
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• 제46권3호
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• pp.549-557
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• 2019

Staphylococcal enterotoxin is a very common cause of food poisoning. Conventional detection methods for the toxin including enzyme-linked immunosorbent assays (ELISAs), chemiluminescence (ECL), and polymerase chain reaction (PCR) assays require a lot of time, efforts, and expert technicians. Lateral flow strip kits have shown great potential for the rapid detection of foodborne pathogens. The lateral flow strip kit is widely used in clinical settings because it is easy to use, fast, and cost effective. A typical lateral flow strip kit uses colloidal gold to generate a visual signal. However, the lateral flow strip kit based on colloidal gold has limited sensitivity to fulfill food safety regulation requirements. This study was performed to develop a rapid test kit for pathogenic staphylococcal enterotoxin B (SEB) in food samples. The rapid detection kit was fabricated based on a nitrocellulose lateral-flow strip. Cellulose nano beads and SEB antibodies were used as the tag and receptor, respectively, to improve the detection performance. Manually spotted SEB antibody and anti-rabbit antibody on the surface of the nitrocellulose membrane were used as test and control spots, respectively. The feasibility of the rapid test kit to detect SEB in samples was evaluated. The sensitivity of the kit was 10 ng/mL SEB spiked in PBS. Additionally, the rapid test kit could detect 1 ng/mL of SEB in chicken meat extract.

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1152-1161
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• 2007

An immunochromatography (ICG) strip test based on a monoclonal antibody for the rapid detection of L. monocytogenes in meat and processed-meat samples was developed in this study. A monoclonal antibody (MAb) specific to L. monocytogenes was produced from cloned hybridoma cells (FKLM-3B12-37) and used to develop an ICG strip test. The antibody showed a stronger binding to L. monocytogenes than other Listeria species, and a weak cross-reaction to S. aureus based on an ELISA. The detection limit of the ICG strip test was $10^5\;cell/ml$. In total, 116 meat and processed-meat samples were collected and analyzed using both the ICG strip test and a PCR. The ICG strip test and PCR indicated L. monocytogenes contamination in 34 and 27 meat samples, respectively. The 7 meat samples not identified as L. monocytogenes positive by the PCR were also tested using an API kit and found to be contaminated by Listeria species. In conclusion, the ICG strip test results agreed well with those obtained using the PCR and API kit. Thus, the developed ICG has potential use as a primary screening tool for L. monocytogenes in various foods and agricultural products, generating results within 20 min without complicated steps.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2002년도 춘계 학술발표회 논문집
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• pp.409-416
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• 2002

기존의 면진 기술을 일반건물에 적용하기 위해서는 낮은 가격과 낮은 무게로 면진 베어링이 제작 및 공급되어야 한다. 이에 본 논문에서는 일반건물에 면진 기술을 적용하기 위한 방법으로 기존의 적층고무 면진 베어링에 철판을 대체하여 섬유로 보강하고, 고무와 섬유의 층으로 구성된 벨트 타입의 면진 베어링을 제안하였다. 또한 제안한 섬유보강 면진 베어링을 설계 및 제작하여 수직실험과 수평실험을 수행하여 그 성능을 검증하였다. 따라서, 스트립형의 면진 베어링이 제작가능하며 일반건물의 필요한 크기로 절단이 가능함을 보였다. 또한 수평 실험 수직 실험을 통해서 기존의 적층고무 면진 베어링을 대체하여 사용할 수 있는 결과를 보였다. 이와 같은 결과를 통해서 일반건물에 낮은 무게와 낮은 가격의 면진 베어링을 공급할 수 있음을 보였다. 이 연구결과로 인해 스트립형의 섬유보강 면진 베어링이 저가건물에 널리 사용될 수 있을 것으로 기대된다.

• 대한의생명과학회지
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• 제4권2호
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• pp.103-112
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• 1998

과산화 효소와 포도당 산화효소의 효소반응을 이용하여 혈액 중 당을 측정하는 혈당 측정 용 스트립을 개발하였다. 멤브레인에 포도당 산화효소와 과산화 효소, 색원체를 건조처리하면 혈당이 멤브레인에 처리된 포도당 산화효소와 즉각 반응하여 과산화 수소를 발생하고 발생된 과산화 수소가 과산화 효소와 반응하여 착색물을 형성한다. 제조된 스트립은 혈액과 접촉하면 반응시간 2∼3분 이내에 0∼800mg/d1의 혈당에 대하여 농도에 따라 연 녹, 청 녹, 짙은 청색을 나타내며 이때 민감도는 40 mg/dl이었다. 육안용 혈당 스트립을 이용하여 정상을 포함한 당뇨 환자의 혈당 농도를 측정한 결과 Ames사와 BM사에서 시판하고 있는 제품과 유사한 결과를 얻을 수 있었다. 본 연구에서 개발된 스트립이 자동 분석기기의 재료로 활용될 수 있는지 여부를 알아보기 위하여 분광 비색계로 발색 반응의 최적 파장을 분석하고 최적 파장에서 각 혈당 농도별 반사 밀도를 측정하여 검정선을 얻었다. 이 검정선에 의해 임상 혈청시료의 혈당 농도를 측정한 결과 일본의 Kyoto Daiichi사의 혈액 분석시스템과 유사한 결과를 얻었다. 이로서 본 연구에서 개발한 혈당 스트립을 이용하여 혈액 중 혈당량을 육안으로 측정할 수 있음은 물론 자동 분석기기의 기본 시료로 이용될 수 있음을 확인할 수 있었다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.273-273
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• 2013

Recently, Point-of-care (POC) testing microdevices enable to do the patient monitoring, drug screening, pathogen detection in the outside of hospital. Immunochromatographic strip (ICS) is one of the diagnostic technologies which are widely applied to POC detection. Relatively low cost, simplicity to use, easy interpretations of the diagnostic results and high stability under any circumstances are representative advantages of POC diagnosis. It would provide colorimetric results more conveniently, if the genetic analysis microsystem incorporates the ICS as a detector part. In this work, we develop a reverse transcriptase-polymerase chain reaction (RT-PCR) microfluidic device integrated with a ROSGENE strip for colorimetric influenza H1N1 virus detection. The integrated RT-PCR- ROSGENE device is consist of four functional units which are a pneumatic micropump for sample loading, 2 ${\mu}L$ volume RT-PCR chamber for target gene amplification, a resistance temperature detector (RTD) electrode for temperature control, and a ROSGENE strip for target gene detection. The device was fabricated by combining four layers: First wafer is for RTD microfabrication, the second wafer is for PCR chamber at the bottom and micropump channel on the top, the third is the monolithic PDMS, and the fourth is the manifold for micropump operation. The RT-PCR was performed with subtype specific forward and reverse primers which were labeled with Texas-red, serving as a fluorescent hapten. A biotin-dUTP was used to insert biotin moieties in the PCR amplicons, during the RT-PCR. The RT-PCR amplicons were loaded in the sample application area, and they were conjugated with Au NP-labeled hapten-antibody. The test band embedded with streptavidins captures the biotin labeled amplicons and we can see violet colorimetric signals if the target gene was amplified with the control line. The off-chip RT-PCR amplicons of the influenza H1N1 virus were analyzed with a ROSGENE strip in comparison with an agarose gel electrophoresis. The intensities of test line was proportional to the template quantity and the detection sensitivity of the strip was better than that of the agarose gel. The test band of the ROSGENE strip could be observed with only 10 copies of a RNA template by the naked eyes. For the on-chip RT-PCR-ROSGENE experiments, a RT-PCR cocktail was injected into the chamber from the inlet reservoir to the waste outlet by the micro-pump actuation. After filling without bubbles inside the chamber, a RT-PCR thermal cycling was executed for 2 hours with all the microvalves closed to isolate the PCR chamber. After thermal cycling, the RT-PCR product was delivered to the attached ROSGENE strip through the outlet reservoir. After dropping 40 ${\mu}L$ of an eluant buffer at the end of the strip, the violet test line was detected as a H1N1 virus indicator, while the negative experiment only revealed a control line and while the positive experiment a control and a test line was appeared.