• 한국ITS학회 논문지
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• 제17권6호
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• pp.40-53
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• 2018

교통카드자료에서 철도이용승객이동은 단말기ID 순서로 나타난다. 최초 단말기ID는 진입역사 Tag-In노선, 최종 단말기ID는 진출역사 Tag-Out노선, 중간 단말기ID는 환승역사 Tag노선정보를 각각 포함한다. 과거 3개 공사기관(서울교통공사, 인천교통공사, 한국철도공사)만 참여하던 수도권도시철도는 최초 및 최종 단말기ID로 표현된 OD만 존재했다. 최근 (주)신분당선, (주)우이-신설경전철 등 민자기관진입으로 진입환승노선 단말기ID와 진출환승노선 단말기ID가 포함된 Chain OD가 보편화되었다. Chain OD를 통한 정확한 경로구축과정은 수도권철도운송기관의 수입금배분의 기초자료로서 매우 중요한 위치를 차지하고 있다. 따라서 지하철네트워크에서 경로탐색은 Chain OD에 대한 최적경로탐색의 문제로 전환되어 새로운 해법이 요구된다. 본 연구는 철도네트워크에서 단말기 Chain OD의 최초 단말기ID와 최종 단말기ID 간의 최적경로탐색기법을 제안하는 것이다. 이때 Chain OD에 민자노선환승 TagIn/Out를 반영하여 최적경로를 탐색하는 방안을 강구한다. 이를 위해 링크표지로 구축된 3가지 경로탐색기법( 1) 노드 - 링크, 2) 링크 -링크, 3) 링크 -노드 )을 순차적으로 적용하는 방안을 제안한다. 가산성경로비용을 토대로 제안된 기법이 최적조건을 만족함을 증명한다.

• 유통과학연구
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• 제19권7호
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• pp.29-39
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• 2021

Purpose: As China experienced a crisis due to Covid-19, the global supply chain collapsed and affected the world. Therefore, it is time for a change in port operational efficiency, increasing in importance with changes in the global supply chain. This study analyzed Shanghai Port's efficiency, the world's largest port and representative hub port in Northeast Asia, by looking at the relationship between facility factors and cargo throughput to present hub port development's timely implications. Research design, data and methodology: This study applied the Charnes, Cooper, and Rhodes (CCR) and Banker, Chames, and Cooper (BCC) models of the data development analysis (DEA) to construct an analysis from the input-oriented and output-oriented perspectives. Results: As a result, Yidong Container Terminal can be considered the most optimized in facilities and operation processes. Yidong and Shengdong Container Terminal should maintain current operating levels, while Pudong Container Terminal should review facility investments. Also, Zhendong, Huong, Mingdong, and Guandong Container Terminal should be reviewed to increase cargo throughput or to adjust current input variables in the current state. Conclusions: Therefore, the utilization of the container terminal input variables should be reviewed, and the factors of inefficiency should be improved. Moreover, the strategic focus of container terminal operations should be on increasing annual cargo throughput.

• 한국경영과학회지
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• 제21권3호
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• pp.215-225
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• 1996

For an undirected stochastic network G, the 2-terminal reliability of G, R(G) is the probability that the specific two nodes (called as terminal nodes) are connected in G. A. typical network reliability problem is to compute R(G). It has been shown that the computation problem of R(G) is NP-hard. So, any algorithm to compute R(G) has a runngin time which is exponential in the size of G. If by some means, the problem size, G is reduced, it can result in immense savings. The means to reduce the size of the problem are the reliability preserving reductions and graph decompositions. We introduce a net set of reliability preserving reductions : the $K^{4}$ (complete graph of 4-nodes)-chain reductions. The total number of the different $K^{4}$ types in R(G), is 6. We present the reduction formula for each $K^{4}$ type. But in computing R(G), it is possible that homeomorphic graphs from $K^{4}$ occur. We devide the homemorphic graphs from $K^{4}$ into 3 types. We develop the reliability preserving reductions for s types, and show that the remaining one is divided into two subgraphs which can be reduced by $K^{4}$-chain reductions 7 polygon-to-chain reductions.

• BMB Reports
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• 제33권2호
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• pp.120-125
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• 2000

M2PI is an active single chain mini-proinsulin with a 9-residue linker containing the turn-forming sequence 'YPGDV' between the B- and A-chains, but which retains about 50% of native insulin receptor binding activity. The refolding efficiency of M2PI is higher than proinsulin by 20-40% at alkaline pH, and native insulin is generated by the enzymatic conversion of M2PI. The solution structure of M2PI was determined by NMR spectroscopy. The global structure of M2PI is similar to that of native insulin, but the flexible linker between the B- and A-chains perturbed the N-terminal A-chain and C-terminal B-chain. The helix in the N-terminal A-chain is partly perturbed and the ${\beta}$-turn in the B-chain is disrupted in M2PI. However, the linker between the two chains was completely disordered indicating that the designed turn was not formed under the experimental conditions (20% acetic acid). Considering the fact that an insulin analogue, directly cross-linked between the C-terminus of the B-chain and the N-terminus of the A-chain, has negligible binding activity, a flexible linker between the two chains is sufficient to keep binding activity of M2PI, but the perturbed secondary structures are detrimental to receptor binding.

• 한국항해항만학회지
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• 제45권3호
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• pp.148-154
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• 2021

컨테이너터미널 운영에서 안벽영역의 장비와 기술이 발전함에 따라 항만운영의 정체가 장치장으로 이전되었다. 따라서 장치장 관리가 터미널 운영 전반에 미치는 영향이 매우 커졌다. 이에 따라 컨테이너터미널 장치장 운영의 최적화를 위한 연구가 많이 수행되었으나 장래 장치장 점유율 변화 자체를 추정한 연구는 없는 실정이다. 본 연구는 부산항 신항을 대상으로 시간의 경과에 따른 확률의 변화를 설명하는 마코프 체인을 적용하여 부산항 신항 컨테이너 터미널의 장치장 점유율 수준에 대한 확률을 분석하였다. 분석결과 부산항 신항의 장치장 점유율은 향후에도 높은 수준을 유지할 확률이 큰 것으로 나타났다.

• Korea-Australia Rheology Journal
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• 제13권4호
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• pp.205-217
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• 2001

This review article summarizes results of recent viscoelastic and dielectric studies for entangled cis-polyisoprene (PI) chains. The PI chains have the so-called type-A dipoles parallel along the chain backbone, and their slow viscoelastic and dielectric relaxation processes reflect the same global chain motion. However, this motion is differently averaged in the viscoelastic and dielectric properties, the former representing the isochronal orientational anisotropy of individual entanglement segments while the latter detecting the orientational correlation of the segments at two separate times (0 and t). On the basis of this difference, the viscoelastic and dielectric data of the entangled PI chains were compared to elucidate detailed features of the chain dynamics. Specifically, the molecular picture of dynamic tube dilation (DTD) incorporated in recent models was tested for linear and star PI chain. The comparison revealed that the DTD picture was valid for linear PI chains but failed for the star PI chains in the dominant part of the terminal relaxation. The failure for the star chains was related to the pre-requisite for the DTD process, rapid equilibration of successive entanglement segments through their constraint release (CR) motion: For the star chains, the dilated tube diameter expected in the terminal regime was considerably large because of a broad distribution of motional modes of the chains, so that the CR-equilibration required for DTD could not occur in time. The terminal relaxation of the star chain appeared to occur through the CR process before the expected DTD process was completed. The situation was different for the linear chain exhibiting narrowly distributed motional modes. The dilated tube expected for the linear chain was rather thin and the required CR-equilibration occurred in time, resulting in the success of the DTD picture. These detailed features of the chain dynamics was revealed only when the viscoelastic and dielectric properties were compared, demonstrating the importance of this comparison.

• Macromolecular Research
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• 제16권7호
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• pp.651-658
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• 2008

Poly(butylene succiate) (PBS), poly(butylene succinate-co-L-lactate) (PBSL), and poly(butylene succinate-co-6-hydroxycaproate) (PBSCL) polymers were degraded by lipase $PS^{(R)}$, and the enzymatic degradation mechanism of PBS was analyzed in detail. The enzymatic degradation of PBS gave 4-hydroxybutyl succinate (4HBS) as the main product. An exo-type hydrolysis mechanism was proposed based on this observation. The terminal chain of PBS had conformational similarity to ordinary tri- and diglycerides and could be incorporated as a substrate in the active site of this lipase. The surface adsorption of the lipase was much larger on PBS and its copolymer films than on the other polyester films because the lipase adhered quite strongly to the polymer terminal through a specific adsorption mechanism. Kinetic analysis showed that the total number of surface adsorption points per unit area of PBSL and PBSCL copolymers was larger than that of the PBS homopolymer.

• Biotechnology and Bioprocess Engineering:BBE
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• 제3권1호
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• pp.40-43
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• 1998

The glycosylation pattern of Erythropoietin (EPO), produced by recombinant CHO cells, was studied using the simple and rapid technique of 'Lectin-blotting'. In this experiment we used three different kinds of lectins, MAA(Maackia amurensis agglutinine), RCA(Ricinus communis agglutinine), and DSA(Datura stramonium agglutinine), which bind to the terminal sialic acid, galactose, and the N-acetyllactosamine chain respectively. The lectin-blotting technique was used to analyze the carbohydrate structure of EPO produced in the presence of two physiologically active chemical compounds, ammonium and chloroquine. The effect of the ammonium ion on the glycosylation of EPO was studied because it accumulated in the medium mainly as a by-product of glutamine matabolism. Ammonium chloride significantly inhibited the sialylation of the terminal galactose residue at concentrations of 8mM or more. Chloroquine, a potent inhibitor of glycosylation, inhibited terminal sialylation at concentrations of 100 and 200 $\mu$M, and at a concentration of 300 $\mu$M, also inhibited Nacetyllactosamine chain synthesis.

• BMB Reports
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• 제29권5호
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• pp.411-416
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• 1996

In order to study the role of the protein shell in both iron uptake and iron core formation of ferritin, we constructed a deletion mutant of the ferritin gene and expressed the mutant gene in Escherichia coli, This mutant was obtained by introducing an amber mutation at position Pro-157 and a deletion of the 19 amino acid residues at the carboxy-terminus of the recombinant tadpole H-chain ferritin. The deleted amino acids correspond to E-helix forming the hydrophobic channel in the protein. E. coli harboring the plasmid pTHP157, which contains the deleted gene, was grown at $23^{\circ}C$ in the presence of 0.1 mM IPTG, and the induced protein appeared to be partly soluble. Nondenaturing polyacrylamide gel electrophoresis showed that the expressed mutant H-chains coassemble into holoprotein, suggesting that E-helix is not necessary for assembly of the subunits as reported for human H-chain ferritin. Its ability in iron core formation was proven in an Fe staining gel, the result disagreeing with the observation that the hydrophobic channel is necessary for iron core formation in human H-chain ferritin.

• 미생물학회지
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• 제35권1호
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• pp.18-24
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• 1999

Kinesin은 Saccharomyces cerevisiae, Aspergillus nidulans, Drosophila melanogaster 등에서 발견 되었으며 dynein 과 더불어 세포분열 과정중 chromosome 의 이동과 spindle pole 의 분리에 관련된 것으로 알려져 있다. 본 연구에서는 기존에 발견된 kinesin 유사 유전자의 homology 가 많은 motor domain을 이용하여 primer를 합성한 후 이를 이용하여 Schizosaccharomyces pombe 로부터 kinesin heavy chain 의 유전자를 클론하였다. 염기서열을 결정한 결과 2496 bp의 해독틀을 가지고 있으며 832개의 아미노산으로 이루어진 분자량이 96 kd의 kinesin heavy chain을 암호화하는 것이 밝혀졌다. 기존에 밝혀진 다른 organism 의 kinesin 과 서열을 비교한 결과 새로이 클론된 S.pombe 의 kinesin 은 C-terminal motor subfamily 에 속함이 밝혀졌다.