• Title/Summary/Keyword: temperature stable characteristics

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Purification and Characteristics of Xylanases from Produced Thermophilic Alkalophilic Bacillus K17 (고온, 알칼리성 Bacillus K17이 생성하는 Xylanase의 정제 및 특성)

  • Kang, In-Soo;Sung, Nack-Kie;Chun, Hyo-Kon;Teruhiko Akiba;Koki Horikoshi
    • Microbiology and Biotechnology Letters
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    • v.14 no.6
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    • pp.447-453
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    • 1986
  • The culture filtrate of thermophilic alkalophilic Bacillus K17 strain contained two types of xylanases were purified by ammonium sulfate fractionation, DEAD-Sephadex A-50 column chromatography, CM-Sephadex C-50 column chromatography and Sephadex G-100 gel filtration. The purified enzymes were found to be homogeneous by sodium dodecyl sulfate and disc polyacrylamide gel electrophoresis. Xylanase I and II were characterized with respect to molecular weight, optimal temperature and pH, thermal and pH stability, and Michaelis constant. Xylanase II was more active and stable, and showed greater substrate affinity and molecular weight than xylanase I. The activities of xylanases I and II were inhibited by Cu$^{++}$, Ag$^+$, Hg$^{++}$ and Fe$^{++}$. Xylanase I hydrolyzed xylan to yield xylobiose and higher amount of xylooligosaccharides, but xylanase II produced xylose other than xylobiose and xylooligosacchrides.

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Distribution Pattern of Deschampsia antarctica, a Flowering Plant Newly Colonized around King Sejong Station in Antarctica (남극 세종기지 주변에 새로이 정착한 현화식물 남극좀새풀 (Deschampsia antarctica)의 개체군 공간분포)

  • Kim, Ji-Hee;Chung, Ho-Sung
    • Ocean and Polar Research
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    • v.26 no.1
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    • pp.23-32
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    • 2004
  • As a baseline survey for long-term monitoring on environmental change around the Antarctic King Sejong Station, distribution pattern of Deschampsia antarctica Desv., a flowering plant newly colonized were investigated qualitatively and quantitatively in both austral summer 2002 and 2003. Dispersal of the seeds and vegetative leaves by skuas might lead to the colonization into this area from neighbors in Maxwell Bay. The pioneer populations were observed around ponds and a stream of the Sejong Point in January 2002, and the maximum dispersal area was four times expanded after a year. Most of the populations were formed on the stable and well-drained substrate, which consisted of moss carpet of Sanionia georgico-uncinata (65%) and pebbles (25%), while only a few young individuals were observed on the unstable and watertight silt-sandy area. Especially, S. georgico-uncinata was being effectively utilized as their primary substrate with the soft, coarse and water-contained leaves. Also the perennial mature plants of D. antarctica were mainly formed on the moss carpet rather than pebbles. A few individuals were grown on other mosses of Polytrichastrum alpinum, Bryum pseudotriquetrum, Pohlia cruda, and Conostomum magellanicum and on a liverwort of Cephaloziella varians. We expect that dispersal of D. antarctica and the following succession to grass field will be countinuously and dynamically proceeded in this area, with the characteristics of ecological niche against the initial moss populations, on the similar continuity of environmental conditions. The continuous observations are needed with establishment of database on environmental change of micro-habitats, e.g. the water content and nutrients of soil and the underground temperature and permafrost.

A DESIGN STUDY OF 100㎓ BAND LOCAL OSCILLATOR SYSTEM BY USING YIG OSCILLATOR (YIG 발진기를 이용한 100㎓ 대역 국부발진 시스템 설계연구)

  • Lee, Chang-Hoon;Kim, K.D.;Kim, H.R.;Jung, M.H.;Han, S.T.;Jae, D.H.;Kim, T.S.
    • Journal of Astronomy and Space Sciences
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    • v.20 no.3
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    • pp.185-196
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    • 2003
  • In this paper, we make a design study for a local oscillator system of the 100 ㎓ band cosmic radio receiving system. We use the YIG oscillator with digital driver which is the main oscillator. This oscillator has a good frequency and phase stability at some temperature variation, and the easy computer aided control characteristics. This total system designed to two subsystem, first is the oscillator system include YIG oscillator, tripler, harmonic mixer and triplexer etc., second is the PLL system to supply the precise and stable local oscillator frequency to mixer. The proposed local oscillator system in this paper can be used in a single or multi pixel receiver because this system can be lock the local oscillator frequency automatically using PC.

Molding Analysis for the Production of Large Sun Visors in Vehicles (차량용 대형 선바이저 생산을 위한 성형해석)

  • Park, Jong-Nam;Noh, Seung-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.11
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    • pp.610-615
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    • 2016
  • Diverse accessories are used in automobiles, such as navigation systems, front and rear cameras, spoilers, and sun visors. Sun visors block sunlight so that drivers can drive safely with a better view, and they are used in many automobile designs. However, when large plastic products are manufactured using injection molding, there are many difficulties that develop, like weld lines, short shots, flow marks, imperfections, and distortion. In this study, a CAE simulation was conducted based on previous results to predict potential problems in the injection molding of large products. The flow characteristics up to complete charge for the melting resins were captured using a computer-aided engineering simulation. The temperature departure on the front part of a flow was about $10^{\circ}C$ and very stable. The practical ejecting time of the cold runner was about 70 seconds in the simulation. Finally, the capability of a suitable injection machine was calculated and recommended by prediction of the injection pressure and the die clamping force.

Prevention of Insulation Damage Layer and Shell Corrosion in Thermal Storage Tanks for District Heating (지역난방용 축열조의 단열재 손상과 외각부식 개선방안)

  • Bang, Yong-Eoon;Yoo, Ho-seon
    • Plant Journal
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    • v.10 no.4
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    • pp.35-41
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    • 2014
  • The height and capacity of the thermal storage tank can be decided by the altitude and heat load of the heat supply area. Evaporation in heat pipe can be prevented by pressurizing it with the hydraulic head of the thermal storage tank. In addition, it absorbs the expanded volume from the temperature changes and supplies water to the pipelines in case of the shortage of water. One of the most important roles of the thermal storage tank is a stable heat supply facility. It can control the heat demand by accumulating the surplus heat and supplying in changing heat demand time. The purpose of this thesis is to be helpful for the operation and maintenance of the thermal storage tanks. The study has been carried out for 18 thermal storage tanks, which have been used polyurethane foam as insulation, among 27 tanks in district heating plants. The characteristics of the insulation materials, the reasons for the damages of the insulation and how impact the insulation damages to the corrosion of the thermal storage tank have been studied.

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Isolation and Characterization of an Alkaline Protease Produced by Bacillus subtilis JK-1 (알칼리성 Pretease를 생산하는 Bacillus subtilis JK-1의 분리 동정 및 효소 특성)

  • Kim, Ji-Yeon
    • Korean Journal of Microbiology
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    • v.43 no.4
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    • pp.331-336
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    • 2007
  • A bacterium producing the alkaline pretense was isolated from Chungkookjoug, and was identified as Bacillus subtilis JK-1 based on morphological, physiological and biochemical characteristics, as well as phylogenetic analysis using 16S rRNA gene sequence. The optimum pH and temperature of the pretense activity were pH 9.0 and $55^{\circ}C$, respectively. This enzyme was stable at the temperatures $40{\sim}80^{\circ}C$. The maximum alkaline pretense production was obtained when 1.0% (w/v) xylose, 1.0% (w/v) yeast extract and 0.3% (w/v) $CuSO_4$ were used as carbon source, nitrogen source and mineral source. Under the optimal condition, growth of the isolate was reached at stationary phase after 12 hr followed by incubation, the alkaline pretense production reached a maximum level with $16{\sim}20$ hr cultivation.

Solid State Cesium Ion Beam Sputter Deposition

  • Baik, Hong-Koo
    • Proceedings of the Korea Association of Crystal Growth Conference
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    • 1996.06a
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    • pp.5-18
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    • 1996
  • The solid state cesium ion source os alumino-silicate based zeolite which contains cerium. The material is an ionic conductor. Cesiums are stably stored in the material and one can extract the cesiums by applying electric field across the electrolyte. Cesium ion bombardment has the unique property of producing high negative ion yield. This ion source is used as the primary source for the production of a negative ion without any gas discharge or the need for a carrier gas. The deposition of materials as an ionic species in the energy range of 1.0 to 300eV is recently recognized as a very promising new thin film technique. This energetic non-thermal equilibrium deposition process produces films by “Kinetic Bonding / Energetic Condensation" mechansim not governed by the common place thermo-mechanical reaction. Under these highly non-equilibrium conditions meta-stable materials are realized and the negative ion is considered to be an optimum paeticle or tool for the purpose. This process differs fundamentally from the conventional ion beam assisted deposition (IBAD) technique such that the ion beam energy transfer to the deposition process is directly coupled the process. Since cesium ion beam sputter deposition process is forming materials with high kinetic energy of metal ion beams, the process provider following unique advantages:(1) to synthesize non thermal-equilibrium materials, (2) to form materials at lower processing temperature than used for conventional chemical of physical vapor deposition, (3) to deposit very uniform, dense, and good adhesive films (4) to make higher doposition rate, (5) to control the ion flux and ion energy independently. Solid state cesium ion beam sputter deposition system has been developed. This source is capable of producing variety of metal ion beams such as C, Si, W, Ta, Mo, Al, Au, Ag, Cr etc. Using this deposition system, several researches have been performed. (1) To produce superior quality amorphous diamond films (2) to produce carbon nitirde hard coatings(Carbon nitride is a new material whose hardness is comparable to the diamond and also has a very high thermal stability.) (3) to produce cesiated amorphous diamond thin film coated Si surface exhibiting negative electron affinity characteristics. In this presentation, the principles of solid state cesium ion beam sputter deposition and several applications of negative metal ion source will be introduced.

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AMOLED Pixel Circuit with Electronic Compensation for Vth and Mobility Variation in LTPS TFTs (LTPS TFT의 Vth와 mobility 편차를 보상하기 위한 AMOLED 화소 회로)

  • Woo, Doo-Hyung
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.46 no.4
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    • pp.45-52
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    • 2009
  • We proposed a new pixel circuit and driving method for the large-area, high-luminance AMOLED applications in this study. We designed with the low-temperature poly-silicon(LTPS) thin film transistors(TFTs) that has poor uniformity but stable characteristic. To improve the uniformity of an image, the threshold voltage($V_{TH}$) and the mobility of the TFTs can be compensated together. The proposed method overcomes the previous methods for mobility compensation, and that is profitable for large-area applications. Black data insertion was introduced to improve the characteristics for moving images. AMOLED panel can operate in two compensation mode, so the luminance degradation by mobility compensation can be released. The scan driver for controlling the pixel circuits were optimized, and the compensation mode can be controlled simply by that. Final driving signal has large timing margin, and the panel operates stably. The pixel circuit was designed for 14.1" WXGA top-emission ANGLED panel. The non-uniformity of the designed panel was estimated under 5% for the mobility compensation time of 1us.

The Strength and Fracture Behavior characteristics of Irradiated Zr-2.5Nb CANDU Pressure Tube Materials (Zr-2.5Nb 중수로 압력관의 조사후 강도 및 파괴거동 특성)

  • An, Sang-Bok;Kim, Yeong-Seok;Kim, Jeong-Gyu
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.25 no.3
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    • pp.510-519
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    • 2001
  • The tensile and fracture toughness tests have been conducted to investigate the degradations of mechanical properties induced mainly by neutron irradiations in Zr-2.5Nb CANDU pressure tube materials operated in Wolsung Unit-1. the tests were performed at room, 150, 200, 250, 300 $\^{C}$ for the irradiated and unirradiated specimens in hot cell. The specimens were directly machined from the tube retaining original curvature using specially designed electric discharge machine(EDM). From the tensile tests of the irradiated specimens, it was found that tensile strength was increased and total elongation was decreased compared to those of the unirradiated ones. The active voltages in the fracture toughness tests for the irradiated showed the discontinuous abrupt increases caused by crack jumping in lower temperature. In the crack resistance curves we found the stable crack growth in the unirradiated, whereas the unstable and three crack growth stages in the irradiated specimens due to the accumulated irradiation defects. The various fracture characteristic values in the irradiated are remarkably lower than those of the unirradiated. Through the fractography, we found in the irradiated that smaller dimple and shorter fissures than the unirradiated, and that the fractured surface had three regions that were flat, transition and slant/shear area. These can explain the difference in the crack growth characteristic values of the irradiated and the unirradiated ones.

Study on characteristics of noncontact vibrating displacement sensor (비접촉식 진동 변위센서의 특성에 관한 연구)

  • Cho, C.W.;Cho, S.T.;Yang, K.H.
    • Journal of Power System Engineering
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    • v.15 no.2
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    • pp.13-18
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    • 2011
  • This thesis is about the result of conducting a specific experiment for the development of noncontact vibration displacement sensor for measuring the spindle vibration that is used for conditional monitoring of machinery. One should be careful when using the eddy current type displacement sensor because the sensitivity of it is different according to the quality of the material. While the probe used for nondestructive inspection adopts the effect of transmitting the material by using the high frequency domain, the eddy current type displacement sensor uses the lower frequency of around 1MHz. Also, while the nondestructive probe uses the method of enhancing output by using the resonance zone, the vibration displacement sensor utilizes the stable zone by avoiding the resonance zone. Since the oscillator of the converter uses the "L" element as Probe, its characteristic changes with the variation of a relevant impedance. In other words, if the length of Probe's Cable gets extended (Impedance increase), the sensitivity declines accordingly. The effect of surrounding temperature was small, but the influence of the quality of Sensor Coil used was high. Moreover, following an experimental demonstration of the phenomenon where the sensitivity decreases as the frequency of the tested material increases from a frequency response test, the maximum frequency that could be measured was approximately 1KHz. It was noted that the degree of precision could be maintained by using the gap of the probe in the linear zone at the installation site.