• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.10
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• pp.1378-1387
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• 2012

To investigate the pharmacological activity of chaga mushroom (Inonotus obliquus) on extraction conditions, chaga was extracted using water (reflux at $50^{\circ}C$, decoction over $90^{\circ}C$, pressure at $121^{\circ}C$) or ethanol (reflux at 50, 70, or $90^{\circ}C$). When water extract was further fractionated into crude polysaccharide (IO-CP), yields of IO-CP (4.8~16.8%) were higher than those of ethanolic extracts (IO-E, 1.9~2.7%) at increased temperature. For antioxidant activity, crude polysaccharide (IO-CP-121) obtained by pressurized extraction showed the highest polyphenolic and flavonoid contents (35.10 mg TAE/g and 18.48 mg QE/g, respectively) as well as DPPH and ABTS free radical scavenging activities (26.08 and 27.99 mg AEAC/100 mg, respectively). Meanwhile, IO-CP-D (decoction) and IO-CP-50 (reflux) had more potent mitogenic effects (2.10- and 1.95-fold of saline control at 100 ${\mu}g/mL$) as well as intestinal immune system modulating activities (6.30- and 5.74-fold) compared to IO-CP-121, whereas ethanolic extracts showed no activity. Although no IO-CP showed cytotoxicity against RAW 264.7 cells at 0.1 mg/mL, IO-CP-121 significantly inhibited TNF-${\alpha}$ and NO production as pro-inflammatory factors in LPS-stimulated RAW 264.7 cells (29.2 and 63.5%, respectively). Ethanolic extracts also showed no cytotoxicity at 0.1 mg/mL, whereas inhibition of TNF-${\alpha}$ and NO production was significantly low compared to that of IO-CP-121. In addition, active IO-CP-D was further fractionated into an unadsorbed (IO-CP-I) and seven adsorbed fractions (IO-CP-II~VIII) by DEAE-Sepharose CL-6B column chromatography in order to isolate immunostimulating polysaccharide. IO-CP-II showed the most potent mitogenic effect and macrophage stimulating activity (4.51- and 1.64-fold, respectively). IO-CP-II mainly contained neutral sugars (61.86%) in addition to a small amount of uronic acid (2.96%), and component sugar analysis showed that IO-CP-II consisted mainly of Glc, Gal, and Man (molar ratio of 1.00:0.55:0.31). Therefore, extraction conditions affect the physiological activity of chaga, and immunostimulating polysaccharide fractionated from chaga by decoction is composed mainly of neutral sugars.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.5
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• pp.330-335
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• 2009

This study was conducted to reclassify Jangho series based on the second edition of Soil Taxonomy : A Basic System of Soil Classification for Making and Interpreting Soil Surveys. Morphological properties of typifying pedon of Jangho series were investigated and physico-chemical properties were analyzed according to Soil Survey Laboratory Methods Manual. The typifying pedon of Jangho series has very dark grayish brown (10YR 3/2) gravelly silt loam A horizon (0~14 cm), very dark brown (7.5YR 3/2) silty clay BAt horizon (14~31 cm), dark brown (7.5YR 3/4) silty clay Bt1 horizon (31~58 cm), brown (7.5YR 4/4) silty clay Bt2 horizon (58~90 cm), and brown (7.5YR 4/4) clay Bt3 (90~120 cm) horizon. That is developed on river terraces. The typifying pedon has an argillic horizon from a depth of 14 to more than 120 cm and a base saturation (sum of cations) of less than 35% at 125 cm below the upper boundary of the argillic horizon. That can be classified as Ultisol. The typifying pedon has 0.9 % or more organic carbon in the upper 15 cm of the argillic horizon, and can be classified as Humult, which is reported for the first time in Korea. That does not have fragipan, kandic horizon, sombric horizon, plinthite, etc. in the given depths, and keys out as Haplohumult. Also that meets the requirements of Typic Haplohumult. That has 35 % or more clay at the particle-size control section, and has mesic soil temperature regime. Jangho series can be classified as fine, mixed, mesic family of Typic Haplohumults, not as fine silty over clayey, mixed, mesic family of Mollic Hapludalfs.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.5
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• pp.393-398
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• 2009

This study was conducted to reclassify Yongdang series based on the second edition of Soil Taxonomy : A Basic System of Soil Classification for Making and Interpreting Soil Surveys. Morphological properties of typifying pedon of Yongdang series were investigated and physico-chemical properties were analyzed according to Soil Survey Laboratory Methods Manual. The typifying pedon of Yongdang series has dark reddish brown (5YR 2/3) silt loam Ap horizon (0~14 cm), dark brown (7.5YR 2/3) silt loam BA horizon (14~32 cm), dark brown (7.5YR 2/3) clay loam Bt horizon (32~57 cm), dark yellowish brown (10YR 4/6) silty clay loam Btx1 horizon (57~110 cm), and dark yellowish brown (10YR 4/6) silty clay loam Btx2 horizon(more than 110 cm). That occurs on gently sloping lava plain and is derived from baslt materials. The typifying pedon has an argillic horizon from a depth of 32 to more than 110 cm and a fragipan from a depth of 57 to more than 110 cm. That has a base saturation (sum of cations) of 35% or more at 75 cm below the upper boundary of the fragipan. That can be classified as Alfisol, not as Inceptisol. The typifying pedon has udic soil moisture regime, and can be classified as Udalf. That has a fragipan with an upper boundary within 100 cm of the mineral soil surface, and keys out as Fragiudalf. Also that meets the requirements of Typic Fragiudalf. That has 18% to 35% clay at the particle-size control section, and has thermic soil temperature regime. Yongdang series can be classified as fine loamy, mixed, thermic family of Typic Fragiudalfs, not as fine loamy, mixed, thermic family of Aquic Eutrudepts.

• Journal of the Society of Disaster Information
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• v.16 no.4
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• pp.701-711
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• 2020

Purpose: In this study, the risk of flammability of a liquid mixture was experimentally confirmed because the purpose of this study was to confirm the increase or decrease of the flammability risk in a mixture of two substances (combustible+combustible) and to present the risk of the mixture. Method: Flash point test method and result processing were tested based on KS M 2010-2008, a tag sealing test method used as a flash point test method for crude oil and petroleum products. The manufacturer of the equipment used in this experiment was Japan's TANAKA. The flash point was measured with a test equipment that satisfies the test standards of KS M 2010 with equipment produced by the company, and LP gas was used as the ignition source and water as the cooling water. In addition, when measuring the flash point, the temperature of the cooling water was tested using cooling water of about 2℃. Results: First of all, in the case of flammable + combustible mixtures, there was little change in flash point if the flash point difference between the two substances was not large, and if the flash point difference between the two substances was low, the flash point tended to increase as the number of substances with high flash point increased. However, in the case of toluene and methanol, the flash point of the mixture was lower than that of the material with a lower flash point. Also, in the case of a paint thinner, it was not easy to predict the flash point of the material because it was composed of a mixture, but as a result of experimental measurement, it was measured between -24℃ and 7℃. Conclusion: The results of this study are to determine the risk of mixtures through experimental studies on flammable mixtures for the purpose of securing the effectiveness of the details of the criteria for determining dangerous goods in the existing dangerous goods safety management method and securing the reliability and reproducibility of the determination of dangerous goods Criteria have been presented, and reference data on experimental criteria for flammable liquids that are regulated in firefighting sites can be provided. In addition, if this study accumulates know-how on differences in test methods, it is expected that it can be used as a basis for research on risk assessment of dangerous goods and as a basis for research on dangerous goods determination.