• Archives of Pharmacal Research
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• 제27권10호
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• pp.1023-1028
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• 2004

A new 12a-dehydrorotenoid 1, 11-dihydroxy-9, 10-methylenedioxy-12a-dehydrorotenoid (1), together with a new isoflavonoid glycoside tectorigenin-7-O-${\beta}$-glucosyl-4'-O-${\beta}$-glucoside (3), were isolated and identified from the rhizomes of I. spuria (Zeal). In addition, 4 known compounds, tectorigenin (2) tectorigenin-7-O-${\beta}$-glucosyl $(1{\leftrightarrow}6)$ glucoside (4), tectoridin (a tectorigenin- 7-O-${\beta}$-glucoside) (5) and tectorigenin-4'-O-${\beta}$-glucoside (6) were isolated and identified for the first time from this plant. The structures of the isolated compounds were determined by spectroscopic methods (UV, IR, $^1H,\;^{13}C$NMR, DEPT, HMQC, NOESY, and HMBC experiments and MS spectrometry) and by comparison with literature data of known compounds. Compounds 2, 4, 5, and 6 are reported for the first time from this plant through the present study.

• Natural Product Sciences
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• 제13권2호
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• pp.101-104
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• 2007

Four isoflavones including tectorigenin (1), irisflorentin (2), irigenin (3), and tectoridin (4) have been isolated from the 70% EtOH extract of Belamcanda chinensis rhizome. The evaluation for protective effect of compounds 1-4 against glutamate-induced cytotoxicity in hippocampal HT22 cell line was conducted. Compound 1 showed significant protective effect with an EC$_{50}$ value of 67.25 ${\pm}$ 1.2 ${\mu}$M, whereas compounds 2-4 were inactive. These results suggest that compound 1 may possess the neuroprotective activity against oxidative cellular injures.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.386.2-386.2
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• 2002

We investigated the effect of tectorigenin (1) with hypoglycemic and hypolipidemic effects on Phase I and II enzymes and TF activity to elucidate the action of an immunosuppressive compound (1) in the diabetic rat. Compound 1 was obtained from the hydrolysis of tectoridin easily isolated from the flower of Pueraria thunbergiana(Leguminosae). Puerariae Flos has been used as therapeutics for diabetes mellitus in traditional medicine of Korea. (omitted)

• Natural Product Sciences
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• 제14권3호
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• pp.187-191
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• 2008

The contents of isoflavones and 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activities were determined in flowers (Puerariae Flos) and roots (Puerariae Radix) of Pueraria thunbergiana to differentiate both pharmacochemical or pharmacognostical usage of both oriental medicinal drugs. The tectorigenin contents in flower before and after acid hydrolysis were shown to be 17.10 ${\mu}mole\;g^{-1}$ and 49.58 ${\mu}mole\;g^{-1}$, respectively, analyzed by HPLC. However, the root displayed much less content of tectorigenin even after acid hydrolysis (6.56 ${\mu}mole\;g^{-1}$) than in the flower. Acid hydrolysis made glycitin and tectoridin of the isoflavone glycosides almost disappear in the flower and root. DPPH assay results demonstrated that acid hydrolysis of both extracts or both glycosides should increase free radical-scavenging activities due to the increase of isoflavone contents. It is also suggested that the flower had much higher concentration of isoflavonoids than in the root.

• Archives of Pharmacal Research
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• 제23권5호
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• pp.461-466
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• 2000

Tectorigenin and kaikasaponin III from the flowers of Pueraria thunbergiana showed potent hypoglycemic and hypolipidemic effects in the streptozotocin-induced diabetic rats. Intraperitoneal administration of these two compounds with 5 and 10 mg/kg, respectively, for seven days to streptozotocin-induced rats significantly reduced the blood glucose, total cholesterol, LDL- and VLDI-cholesterol and triglyceride levels when compared with those of control group. Glycitein in which 5-OH is unlinked and tectoridin (7-O-glycoside of tectorigenin) isolated from the flowers of P. thunbergiana did not improve hyperglycemia and hyperlipidemia. In addition, tectorigenin showed in vitro antioxidant effects on 1,1-diphenyl-B-pirylhydrazyl (DPPH) radical, xanthine-xanthine oxidase superoxide anion radical, and lipid peroxidation in rat microsomes induced by enzymatic and non-enzymatic methods. We further found that tectorigenin and kaikasaponin III protected the Vero cell line(normal monkey kidney) from injury by hydrogen peroxide. From these findings, it seems likely that the antioxidant action of tectorigenin and kaikasaponin III may alleviate the streptozotocin-induced toxicity and contribute to hypoglycemic and hypolipidemic effects.

• Natural Product Sciences
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• 제21권1호
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• pp.49-53
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• 2015

Eight isoflavonoid compounds were isolated from the EtOAc fraction of Maackia amurensis which had shown the highest anti-Helicobacter pylori activity among the fractions, using medium pressure liquid chromatography and recrystallization. Based on the spectroscopic data including $^1H$-NMR, $^{13}C$-NMR, HMBC and MS data, the chemical structures of the isolates were determined to be (-)-medicarpin (1), afromosin (2), formononetin (3), tectorigenin (4), prunetin (5), wistin (6), tectoridin (7) and ononin (8). Anti-H. pylori activity of each compound was evaluated with broth dilution assay. As a result, (-)-medicarpin (1), tectorigenin (4) and wistin (6) showed anti-H. pylori activity. (-)-Medicarpin (1) exhibited the most potent growth inhibitory activity against H. pylori with the minimal inhibitory concentration $(MIC)_{90}$ of $25{\mu}M$, and tectorigenin (4) with $MIC_{90}$ of $100{\mu}M$ ranked the second. This is the first study to show the anti-H. pylori activity of M. amurensis, and it is suggested that the stem bark of M. amurensis or the EtOAc fraction or the isolated compounds can be a new natural source for the treatment of H. pylori infection.

• 공업화학
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• 제29권6호
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• pp.772-781
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• 2018

본 연구에서는 범부채 뿌리 50% 에탄올 추출물 및 에틸아세테이트 분획물을 제조하고 이들의 항산화 및 항균 활성, 세포 보호 효능을 평가하였다. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) 자유라디칼 소거 활성($FSC_{50}$) 측정 결과, 50% 에탄올 추출물은 $621.5{\mu}g/mL$, 에틸아세테이트 분획물은 $253.0{\mu}g/mL$이었다. Luminol 발광법을 이용한 총 항산화능($OSC_{50}$)은 추출물과 분획물에서 각각 13.6 및 $3.0{\mu}g/mL$이었다. 항균 활성 측정에서 Staphylococcus aureus 및 Candida albicans에 대한 에틸아세테이트 분획물의 최소저해농도(minimum inhibitory concentration, MIC)는 각각 156 및 $1,250{\mu}g/mL$으로 나타났으며, 화장품에 사용하는 기존 방부제인 methyl paraben보다 유사하거나 더 높은 활성을 보여주었다. $^1O_2$로 유도된 세포 손상에 대한 보호 효과(${\tau}_{50}$)에서 50% 에탄올 추출물은 $4{\sim}64{\mu}g/mL$ 농도 범위에서 농도 의존적으로 세포 보호 활성을 나타냈다. $16{\mu}g/mL$ 농도에서 50% 에탄올 추출물, 에틸아세테이트 분획물 및 (+)-${\alpha}$-tocopherol의 ${\tau}_{50}$은 각각 36.4, 45.0 및 45.8 min이었으며, 에틸아세테이트 분획물은 $^1O_2$로 유도된 세포 손상에서 (+)-${\alpha}$-tocopherol과 유사한 세포 보호 활성을 나타냈다. UVB로 유도된 HaCaT 세포 손상에서 에틸아세테이트 분획물은 $8{\mu}g/mL$에서 세포 내 활성산소종(reactive oxygen species, ROS)을 최대 45.9%까지 감소시켰다. 과산화수소로 유도된 HaCaT 세포 손상에서도 에틸아세테이트 분획물은 $0.5{\sim}8.0{\mu}g/mL$에서 세포 생존율을 유의적으로 증가시켰다. 범부채 뿌리 에틸아세테이트 분획물의 성분 분석 결과, irisflorentin, irigenin, tectorigenin, resveratrol, iridin 및 tectoridin 등의 플라보노이드 및 폴리페놀 성분이 확인되었다. 결론적으로 범부채 뿌리 추출물 및 분획물은 화장품의 천연 항산화 및 항균 소재로서 적용 가능성이 있음을 시사한다.