• 약학회지
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• 제44권1호
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• pp.60-65
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• 2000

The yeast extract, coconut water, safflower seed oil, arachidonic acid, linolenic acid, jasmonic acid and methyl jasmonate were added to Gamborg's B$_{5}$ medium. The changes on productivity of baccatin III were estimated every 30 minutes and the results were compared using the selected high yielding cell culture system of Taxus cuspidata. In most cases, the peaks of baccatin III productivity occured at 90~120 min after addition of elicitors. Among the compound elicitors, safflower seed oil showed the highest productivity of baccatin III. Also arachidonic acid and linolenic acid increased the baccatin III production.

• 생약학회지
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• 제29권4호
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• pp.360-364
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• 1998

To develop new elicitors inducing the high productivity of taxane derivatives, plant growth inhibitors, namely, maleic acid hydrazide, N-phosphomethyl glycine and succinic acid 2.2-dimethyl hydrazide, coconut milk and yeast extract were administrated in the cell suspension culture system of Taxus cuspidata, and the production of baccatin III were analysed. The effects of amino acid related with the biosynthesis of baccatin III were also examined in these culture system. As the results, a remarkable enhancement of baccatin III production was observed in the cultivation with coconut water and with maleic acid hydrazide.

• Journal of Microbiology and Biotechnology
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• 제11권2호
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• pp.204-210
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• 2001

Paclitaxel can be produced in high yield and with a high degree of purify from plant cell cultures of Taxus chinensis. The complete purification method was systematically established and described. This method was an efficient procedure for the purification of paclitaxel from crude paclitaxel, consisting or reverse-phase chromatography, followed by a normal-phase chromatography. The two-stage HPLC purification scheme serves as an effective and economical approach for resolving paclitaxel from complex mixtures of taxoids, with high purify (>99%) and low impurities (<0.1%). The process is readily scalable to a pilot plant and eventually to a production environment where multikilogram quantities of material are expected to be produced. The process has been optimized to minimize solvent usage, complexity, and operating costs.

• Journal of the Korean Wood Science and Technology
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• 제25권2호
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• pp.45-51
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• 1997

주목 잎을 채취하여 건조하고 아세톤-물 (7 : 3, v/v) 의 혼합액으로 추출한 후 에틸아세테이트 및 수용성 부분으로 분리하고 Sephadex-LH 20으로 충진한 칼럼을 이용하여 2개의 flavan 단량체와 2개의 후라보노이드 배당체를 단리하였다. 에틸아세테이트용성 추출물은 대부분 (+)-catechin 과 (-)-epicatechin으로 구성되어 있었으며 수용성 부분에서는 quercetin-3-0-arabinopyranosyl-($1"'{\rightarrow}6"$)-${\beta}$-D-glucoside와 quercetin-3-O-rutinoside 인 두 개의 탄수화물로 구성된 배당체를 분리하였으며 주목에서는 이들 화합물은 아직 보고된 바가 없다. 이들의 구조결정을 위하여 박층크로마토그래피를 실시하고 $^1H$-NMR과 $^{13}C$-NMR 스펙트럼을 기존의 스펙트럼과 비교, 분석하여 정확한 구조를 규명하였다.

• Mycobiology
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• 제39권3호
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• pp.151-157
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• 2011

The endophytic fungal populations of different tissues of Taxus baccata grown at high altitudes in West Bengal, India were explored. These isolated fungal populations represented different genera, which were screened for taxol production using immunoassay technique. The culture AAT-TS-$4_1$ that produced taxol was identified as Gliocladium sp. based on its cultural, morphological characteristics, internal transcribed spacer, and 18S rRNA sequence analysis. Kinetics of taxol production as a function of culture growth were investigated.

• 한국균학회지
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• 제51권4호
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• pp.419-428
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• 2023

Several endophytic fungal strains were isolated from Taxus cuspidata and identified by molecular analysis of the internal transcribed spacer and RNA polymerase II second largest subunit. This study aimed to determine the relative abundance and compare the species diversity of endophytic fungal communities within needle leaves and twigs. We identified a total of 49 endophytic fungal species. Notably, two species, Trichoderma dingleyae and Xylaria cubensis, were discovered to be previously unrecorded in Korea. The fungal communities in both plant tissues demonstrated distinct species composition. Differences were observed in the relative abundance and species diversity index between needle leaves and twigs. Our findings suggest that the host plant tissues influence the species diversity of endophytic fungal communities.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1994년도 한국생물과학협회 학술발표대회
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• pp.186.2-186
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• 1994

No Abstract, See Full Text

• 환경영향평가
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• 제23권2호
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• pp.119-136
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• 2014

This work aims to select the potentially vulnerable plant species against climate change at alpine and subalpine belts of Mts. Sorak, Jiri, and Halla, from central, southern, southern insular high mountains of the Korean Peninsula, respectively. The selection of global warming related vulnerable plants were performed by adapting various criteria, such as flora, endemicity, rarity, floristically specific and valuable species, species composition at mountain summits, horizontal and vertical ranges of individual species, and their distributional pattern in the Korean Peninsula. Line and quadrat field surveys along the major trails from all directions at height above 1,500 meters above sea level of Mts, Sorak, Jiri and Halla were conducted each year during spring, summer, and autumn from 2010 to 2011. Based upon above mentioned eight criteria, high level of climate change related potentially vulnerable arboral plants, such as Rhododendron aureum, Taxus caespitosa, Pinus pumila, Oplopanax elatus, Vaccinium uliginosum, and Thuja koraiensis are noticed from at subalpine belt of Mt. Sorak. Species of Abies koreana, Rhododendron tschonoskii, Oplopanax elatus, Taxus cuspidata, Picea jezoensis, and Juniperus chinensis var. sargentii belong to climate change concerned vulnerable species at subalpine belt of Mt. Jiri. High level of climate change related species vulnerability is found at alpine and subalpine belts of Mt. Halla from Diapensia lapponica var. obovata, Salix blinii, Empetrum nigrum var. japonicum, Vaccinium uliginosum, Juniperus chinensis var. sargentii, Taxus cuspidata, Rhamnus taquetii, Abies koreana, Hugeria japonica, Prunus buergeriana, and Berberis amurensis var. quelpartensis. Countermeasures to save the global warming vulnerable plants in situ are required.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.672-685
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• 2003

Yew (Taxus cuspidata Sieb.) chose that grow as medicine, food, decorative plant in Korea's Kyong-Gi province surroundings. Extracts of yew extracted leaf of 250 g and stems of 300 g with 1,3-butylene glycol (1,3-BG), propylene glycol (PG) and water. As results, external appearance of leaf extract of yew was slightly brown clear extract. The pH was 5.3$\pm$0.5, and specific gravity was 1.012$\pm$0.05, and refractive index was 1.375$\pm$0.05. Also, appearance of stem's extract was slightly brown clear extract, and the pH was 5.4$\pm$0.5, and specific gravity was 1.016$\pm$0.05, and refractive index was 1.358$\pm$0.05. Oil of yew separated from seeds, and extracted polysaccharide high purity from fruits. As a result, specific gravity of oil was 0.987, and obtained 40％ of yield. Total polyphenols amount of yew extract is detected 0.563％ in leaves, 0.325％ in stems, whereas total tannins amount contained 0.054％ and 0.037％ each in leaves and stems. As effect in cosmetics, the anti-oxidative effect by DPPH method is 75.0％ in leaves, and stems was 64.0％. Collagen synthesis rate was shown high activity by 54.16％ in stem's extract, 33.18％ in leaves' extract. Also, PPE-inhibitory activities were 13.7％ and 23.5％ each in leaves and stems. Anti-inflammatory effect of yew seed oil displayed superior effect of 41 ％ than control. Polysaccharide's molecular weight that is gotten from fruits was 5$\times$10$^4$~ 3$\times$10$^{5}$ dalton, and got 20.0$\pm$5％ of yield.

• KSBB Journal
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• 제14권6호
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• pp.665-671
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• 1999

본 연구에서는 식물 유래의 항암제인 taxol을 생산하는 주목세포의 현탁배양을 통하여 taxol 반합성의 전구체인 10-deacetylbaccatin III(10-DAB)의 생산을 증대시키고자 하였다. 이 차대사산물의 생산이 세포생장과 관련이 있으므로 세포생장이 우수한 배지를 선택하기 위한 실험을 수행하였고, SH 배지에서 가장 우수한 세포생장이 관찰되었다. 또한 선택된 SH 배지에서 우수한 세포생장과 10-DAB 생산을 얻기 위해 초기 접종농도와 당농도의 변화를 관찰하였다. 초기 접종농도와 당농도가 낮을수록 세포생장이 빠르며, 낮은 초기 당농도와 초기 접종농도 6 g/L FCW 이었을 때 10-DAB의 생산이 우수하였다. 생산성을 높이기 위한 고농도 세포생장과 배지로의 10-DAB 분비를 목적으로 perfusion culture를 도입하였다. 고농도 당을 첨가한 배지로 perfusion을 수행한 결과 배양 후반까지 세포생장이 가능하였고, FCW/DCW ratio가 감소되어 대조구의 2.5배인 34.67 g/L의 높은 세포농도를 얻을 수 있었다. 10-DAB의 생산은 perfusion 시작후 10일 동안은 배지로 배출된 양이 세포내보다 높았다.