• Title/Summary/Keyword: taxonomic composition

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Evidence for Differentiation of Colon Tissue Microbiota in Patients with and without Postoperative Hirschsprung's Associated Enterocolitis: A Pilot Study

  • Arbizu, Ricardo A.;Collins, David;Wilson, Robert C.;Alekseyenko, Alexander V.
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.24 no.1
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    • pp.30-37
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    • 2021
  • Purpose: To investigate the differences in the colon microbiota composition of Hirschsprung's disease (HSCR) patients with and without a history of postoperative Hirschsprung's associated enterocolitis (HAEC). Methods: Colon tissue microbiota was characterized by bacterial deoxyribonucleic acid (DNA) extraction and 16S rDNA sequencing for taxonomic classification and comparison. Results: The sequence diversity richness within samples was significantly higher in samples from patients with a history of postoperative HAEC. We observed an increased relative abundance of the phyla Bacteroidetes, Firmicutes and Cyanobacteria in HAEC patients and Fusobacteria, Actinobacteria and Proteobacteria in HSCR patients and, an increased relative abundance of the genera Dolosigranulum, Roseouria and Streptococcus in HAEC patients and Propionibacterium and Delftia in HSCR patients. Conclusion: Our findings provide evidence that the colon tissue microbiota composition is different in HSCR patients with and without postoperative HAEC.

Investigation of Fungal Strains Composition in Fruit Pollens for Artificial Pollination

  • Do, Heeil;Kim, Su-Hyeon;Cho, Gyeongjun;Kim, Da-Ran;Kwak, Youn-Sig
    • Mycobiology
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    • v.49 no.3
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    • pp.249-257
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    • 2021
  • Plants pollination are conducted through various pollinators such as wind, animals, and insects. Recently, the necessity for artificial pollination is drawing attention as the proportion of natural pollinators involved is decreasing over the years. Likewise, the trade in pollen for artificial pollination is also increasing worldwide. Through these imported pollens, many unknown microorganisms can flow from foreign countries. Among them, spores of various fungi present in the particles of pollen can be dispersed throughout the orchard. Therefore, in this study, the composition of fungal communities in imported pollen was revealed, and potential ecological characteristics of the fungi were investigated in four types of imported pollen. Top 10 operational taxonomic unit (OTU) of fungi were ranked among the following groups: Alternaria sp., Cladosporium sp., and Didymella glomerata which belong to many pathogenic species. Through FUNGuild analysis, the proportion of OTUs, which is assumed to be potentially plant pathogens, was higher than 50%, except for apple pollen in 2018. Based on this study of fungal structure, this information can suggest the direction of the pollen quarantine process and contribute to fungal biology in pollen

Bacterial Diversity at Different Depths in Lead-Zinc Mine Tailings as Revealed by 16S rRNA Gene Libraries

  • Zhang, Han-Bo;Shi, Wen;Yang, Ming-Xia;Sha, Tao;Zhao, Zhi-Wei
    • Journal of Microbiology
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    • v.45 no.6
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    • pp.479-484
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    • 2007
  • Bacterial communities at 10 cm, 100 cm, and 200 cm depths in a 100-year-old lead-zinc tailing heap were evaluated by constructing 16S rRNA gene libraries. In total, 98 operational taxonomic units (OTUs) were identified from 193 clones at a 3% sequence difference level. The OTU number and species richness decreased with the depth. Species composition was significantly different between the three libraries. Fifty-seven percent of the examined clones were Acidobacteria and 27% belonged to Proteobacteria. Other sequences included Chloroflexi, Firmicutes, Chlamydiae, Actinobacteria, Gemmatimonadetes, Nitrospira, and three unclassified OTUs. Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria were mainly distributed in the rhizosphere of naturally colonizing plants; however, Deltaproteobacteria, Acidobacteria, and Chloroflexi tended to inhabit the deeper tailings (below the 100 cm-depth).

SIZE AND ALBEDO PROPERTIES OF MAIN BELT ASTEROIDS BASED ON THE COMPARATIVE STUDY OF INFRARED ASTEROID SURVEYS: IRAS, AKARI, AND WISE

  • Usui, Fumihiko;Hasegawa, Sunao;Ishiguro, Masateru;Muller, Thomas G.;Ootsubo, Takafumi
    • Publications of The Korean Astronomical Society
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    • v.32 no.1
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    • pp.55-57
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    • 2017
  • Presently, the number of known asteroids is more than 710,000. Knowledge of size and albedo is essential in many aspects of asteroid research, such as the chemical composition and mineralogy, the size-frequency distribution of dynamical families, and the relationship between small bodies in the outer solar system or comets. Recently, based on the infrared all-sky survey data obtained by IRAS, AKARI, and WISE, the large asteroid catalogs containing size and albedo data have been constructed. In this paper, we discuss the compositional distribution in the main belt regions based on the compiled data on size, albedo, and separately obtained taxonomic type information.

Isolation and identification of cellulolytic Actinomycetes (Cellulose 분해 방선균의 분리 및 동정)

  • 정현호;성하진;최용진;양한철
    • Microbiology and Biotechnology Letters
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    • v.14 no.5
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    • pp.377-383
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    • 1986
  • About 300 cellulolytic actinomycetes isolated from soils were tested for their cellulase activities estimated by means of filter paper swelling and carboxymethyl cellulose saccharifying activity. Then, 16 isolates which had shown relatively high levels of CMCase activity were selected and examined for their abilities of $\beta$-glucosidase production. Among them strain No. 109 was found to have highest level of intracellular $\beta$-glucosidase, and selected for the further studies. In this paper, the cultural, morphological and physiological properties, and cell wall composition of strain No. 109 were described in relation to the taxonomic status of this actinomycete. Based on the results obtained in these experiments strain No. 109 was identified to be a similar species to Streptomyces tanashiensis.

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Identification of Adenosine Deaminase Inhibitor-producing Bacterium Isolated from Soil

  • SHIN, YONG KOOK;YONG-HA PARK;JAE-DONG LEE;HONG-KI JUN
    • Journal of Microbiology and Biotechnology
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    • v.7 no.1
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    • pp.32-36
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    • 1997
  • An adenosine deaminase inhibitor-producing bacterium was isolated from soil. An isolate exhibiting high adenosine deaminase inhibitory activity, was designated J-89, and classified as a strain of Bacillus subtilis on the basis of its morphological, phenotypic characteristics, the menaquinone content and cellular fatty acid composition. To confirm the taxonomic position of the strain we need more information such as DNA-DNA homology and other chemotaxonomic characteristics. In this paper we provisionally named strain J-89 as Bacillus sp. J-89 pending further chemotaxonomic study and analysis of adenosine deaminase inhibitor.

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Morphological variation of Scenedesmus quadricauda (Turpin) de Br bisson and Scenedesmus armatus (Chodat) G.M. Smith in culture (내실배양에 따른 Scenedesmus quadricauda(Turpin) de Br bisson과 Scenedesmus armatus(Chodat) G.M. Smith의 형태변이)

  • 안선숙
    • Journal of Plant Biology
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    • v.28 no.4
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    • pp.305-315
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    • 1985
  • Morphological variations within the species of Scenedesmus in Korea, S. armatus and 2 strains of S. quadricauda were investigated. Taxonomic characters such as length of long spine, ridge, unicell-colony transformation, and colony types were compared. The length of long spine was constant, whereas the distribution of the spine varied with the age of culture. Ridge and short spine in S. armatus occurred constantly, whereas those in S. quadricauda varied with the composition of the media and age of culture. Strains CY-1 and CY-2 showed 6 different colony types in the stationary culture, whereas S. armatus formed only two types, armatus and armatus-longus types. All strains showed unicell-colony transformation. The cell became minimum when the daughter cell was released, and reached maximum at division.

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Anatomical Comparison of North American Eastern, Southern, and Western Redcedar Wood

  • Eom, Young Geun;Kwon, Ohkyung;Hanna, Robert B.;Meyer, Robert W.
    • Journal of the Korean Wood Science and Technology
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    • v.36 no.2
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    • pp.9-20
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    • 2008
  • Anatomical comparison of 3 North American species with commercial name of redcedar was executed to provide taxonomic information for prevention of confusion and establishment of sound business transaction in the wood market. Eastern redcedar (Juniperus virginiana) and southern redcedar (Juniperus silicicola) could not be separated on the basis of microstructure and ultrastructure of wood. Western redcedar (Thuja plicata), however, appeared to be obviously separated from eastern and southern redcedar in a number of microscopic and ultramicroscopic features. Useful features for separating these two groups were intercellular spaces in transverse surface, latewood width, radial intertracheid pitting, warty layer and pits with torus extensions in tracheids, ray composition, nodular end walls in ray parenchyma cells, and cross-field pitting.

The Marine Decapod Crustaceans of Geojedo Island and Its Adjacent Islets, Korea (거제도의 해산 십각류)

  • Sa Heung Kim;Won Kim
    • Animal Systematics, Evolution and Diversity
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    • v.14 no.3
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    • pp.293-309
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    • 1998
  • A taxonomic study for clarifying the decapod fauna of Gejedo Island, Korea was performed. Seventy nine species in 28 families were identified through the present investigation and of which 9 species of shrimps, 7 species of anomurans, and 39 species of crabs are newly added to the decapod fauna of this area. With the previously known 40 species, a total of 93 decapod species from this island and its adjacent islets are listed, and Pugettia quadridens intermedia Sakai, 1939 is redescribed with illustrations. The zoogeographical aspects of Geojedo Island are also discussed based on the composition of geographical forms of brachyuran decapods.

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Complete Mitochondrial Genome of Martes flavigula (Carnivora: Mustelidae) and Its Phylogenetic Status in the Genus Martes

  • Han-Na Kim;Yeong-Seok Jo
    • Animal Systematics, Evolution and Diversity
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    • v.40 no.2
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    • pp.147-149
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    • 2024
  • We report the complete mitochondrial genome sequence of endangered yellow-throated marten, Martes flavigula. The complete mitochondrial genome of M. flavigula is 16,555 bp in length. We identified 13 protein coding genes, 22 transfer RNA, two ribosomal RNA, and one control region. The mitogenome is A+T rich, with a composition of 31.3% A, 28.7% C, 13.0% G, and 27.0% T. According to phylogenetic analysis based on mitochondrial complete genomes, Martes flavigula in the subgenus Charronia was clearly distinct from the subgenus Martes. This phylogeny of the genus Martes supports the conventional systematic treatment. The genetic and taxonomic analysis in this study provides necessary information for the future studies of yellow-throated marten and the Mustelidae family.