• 제목/요약/키워드: tassel

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Application of genotyping-by-sequencing (GBS) in plant genome using bioinformatics pipeline

  • Lee, Yun Gyeong;Kang, Chon-Sik;Kim, Changsoo
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.58-58
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    • 2017
  • The advent of next generation sequencing technology has elicited plenty of sequencing data available in agriculturally relevant plant species. For most crop species, it is too expensive to obtain the whole genome sequence data with sufficient coverage. Thus, many approaches have been developed to bring down the cost of NGS. Genotyping-by-sequencing (GBS) is a cost-effective genotyping method for complex genetic populations. GBS can be used for the analysis of genomic selection (GS), genome-wide association study (GWAS) and constructing haplotype and genetic linkage maps in a variety of plant species. For efficiently dealing with plant GBS data, the TASSEL-GBS pipeline is one of the most popular choices for many researchers. TASSEL-GBS is JAVA based a software package to obtain genotyping data from raw GBS sequences. Here, we describe application of GBS and bioinformatics pipeline of TASSEL-GBS for analyzing plant genetics data.

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광평옥과 다청옥의 수이삭과 수염에서 안토시아닌 생합성 유전자 발현 분석 (Expression Analysis of Anthocyanin Biosynthetic Genes of Tassel and Silks in Gwangpyeongok and Dacheongok)

  • 고영삼;배환희;최유찬;손재한;하준영;신성휴;정태욱
    • 한국작물학회지
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    • 제66권3호
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    • pp.240-247
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    • 2021
  • 본 실험은 광평옥과 다청옥 품종의 수이삭과 수염을 대상으로 안토시아닌 생합성 관련 분자생물학적 및 이화학적 특성을 검토하여 향후 기능성 옥수수 개발에 필요한 기초자료로 활용하고자 수행되었다. 1. 광평옥에서는 수이삭과 수염에서 모두 안토시아닌이 형성되지 않은 반면, 다청옥에서는 모두 형성되었다. 전체 안토시아닌 함량은 수이삭과 수염에서 모두 다청옥이 광평옥에 비해 30배 정도 높았다. 또한, 안토시아닌 성분은 다청옥의 수이삭에서 C-3-G만 측정되었고, 수염에서는 각각 C-3-G는 45.2배, Pg-3-G는 27.3배, M-3-G는 37.6배 광평옥에 비해 더 검출되었다. 2. 광평옥의 수이삭에서 F3'H, DFR, GST 유전자가 발현이 감소하였고, 수염에서는 F3'H와 DFR 유전자가 발현이 감소하였다. 반대로 다청옥에서는 수염에서 DFR 유전자만 발현이 감소되었고, 나머지 유전자들은 정상적으로 발현되었다. 또한 안토시아닌 생합성 유전자들의 발현을 조절하는 전사조절인자는 광평옥 수이삭에서는 P1이 수염에서는 R1이 관여한다는 사실을 확인하였다. 3. 광평옥의 수이삭과 수염에서 다청옥에 비해 각각 linoleic acid (C18:2)가 6.6%, 10.9% 감소하고, linolenic acid (18:3)는 8.5%, 8.5% 증가하였다. 또한, 광평옥의 수염에서는 palmitic acid (C16:0)가 4.1% 증가하고, oleic acid (C18:1)는 다청옥에 비해 2.1% 감소하였다. 그러나 stearic acid (18:0)는 수이삭과 수염에서 성분 변화가 전혀 없었다. 그리고 광평옥의 수이삭과 수염의 전체 지방산 함량은 광평옥에 비해 각각 10.3%, 30.4% 증가하였고, 각 지방산 성분과 함량 분석은 유사한 결과를 보여주었다. 그러나 phytosterol 성분 분석에서는 유의미한 결과를 확인할 수가 없었다.

파종양식과 재식밀도가 옥수수 변형단교잡종 교배친의 생육 및 채종량에 미치는 영향 (Effects of Planting Pattern and Plant density on Seed Production of a Modified Single Cross Corn Hybrid)

  • 박근용;강영길;박승의
    • 한국작물학회지
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    • 제29권1호
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    • pp.62-66
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    • 1984
  • 옥수수 변형단교잡종 채종재배에 있어서 웅주간파 ♂♀ 1 : 2, 2 : 4 등의 파종양식과 재식밀도가 종자친의 생육 및 채종량과 화분친의 웅수특성에 미치는 영향을 구명하고자 1983년 제천옥의 종자친 KS8/B68과 화분친 KS5를 공시하여 시험한 결과를 요약하면 다음과 같다. 1. 개화기 및 간장은 파종양식이나 재식밀도에 크게 영향을 받지 않았고 종자친의 착수고는 파종양식간에는 차이가 없었으나 재식밀도가 증가함에 따라 직선적으로 증가하였다. 2. 종자친의 100주당 수수는 10a당 종자친 3,500본에서는 파종양식간에 비슷하나 5,000본 이상에서는 2 : 4 재배보다 웅주간파나 1 : 2재배에서 많았으며 재식밀도 증가에 따라 파종양식에 관계없이 직선적으로 감소하였다. 수당입수는 파종양식간에는 차이가 없고 파종양식과 재식밀도의 상호작용도 유의하지 않았으나 재식밀도 증가에 따라 작아졌다. 100입중은 25g 내외로 처리간 차이가 없었다. 3. 화분친의 화분이 제한되지 않은 조건에서의 채종량은 웅주간파, 1 : 2재배, 2 : 4재배순으로 많은 경향이였고 10a당 종자 친 3,500본에서 5,000본으로 증가시킴에 따라 크게 증가하나 5,000본에서 6,500본으로 재식밀도 증가에 따른 채종량 증가는 크지 않았다. 4. 화분친의 웅수장과 웅수당 영화수는 1 : 2나 2 :4재배에 비하여 웅주간파 재배에서 작았고 재식밀도 증가에 따라 1 : 2 및 2 : 4재배에서는 크게 감소하였으나 2: 4재배에서는 유의하게 작아지지 않았다. 5. 변형단교잡종의 채종에는 1 : 2나 2 : 4재배의 10a당 종자친 5,000본 재식이 적합할 것으로 생각된다.

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Post-modern 복식의 복고성에 관한 연구-Baroque 복식의 loop와 tassel을 중심으로-

  • 정흥숙
    • 복식
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    • 제25권
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    • pp.71-83
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    • 1995
  • The purpose of this study was to analyze the formative costume of Baroque style and to in-vestigate the characteristic of loop and tassel which are the main factors of decoration in the Post-modern clothing in point of the characteristic of retro. For this purpose, theoretical studies about the concepts of Post-modernism and aesthetic sphere o Baroque style were preceeded. Especially the parody which is concept that is the most influencing to the contemporary art, architecture, and costume. It is the identity of the parody that is being understood smoothly the creation and the imi-tation which is contrasted each other. The parody is urging the purpose rather than the essence and the recreation rather than the originality of the original works. The synthetic results were as follows ; 1. The frequent usage of loop and tassel which was expressed in the first half of 1990's in the modern clothing influenced the aes-thetic will of aspiration about the past. 2. The characteristic of retro to the tra-ditional form constitutes a base of spiritual thinking of Post-modernism which has been expressed in 1970's afterwards. The peculiarity about the great change in the traditional concept of artistic creation in the Post-modern clothing is the very extention of a range of application about the retro in which it becomes the essence or the oppor-tunity in artistic creation.

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유전체 서열 재사용을 이용한 Genotyping By Sequencing 기술의 단일 염기 다형성 탐지 효율 개선 (Improvement of SNPs detection efficient by reuse of sequences in Genotyping By Sequencing technology)

  • 백정호;김도완;김준아;이태호
    • 한국정보통신학회논문지
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    • 제19권10호
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    • pp.2491-2499
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    • 2015
  • 개별 생물의 유전적 특성인 유전형 정보를 얻기 위한 개발된 기법들 중 현재 가장 많이 사용되고 있는 것은 차세대 염기서열결정을 통해 얻어진 서열을 분석하여 단일핵산염기다형현상 기반의 유전형 정보를 얻어내는GBS 방법이다. 현재 TASSEL은 GBS방법을 통해 얻어진 서열을 분석하여 시료의 유전형을 측정하기 위해 가장 많이 사용되고 있는 프로그램 중 하나이다. 그러나 TASSEL은 염기서열결정을 통해 얻어진 서열 중 일부만을 사용하는 한계가 존재한다. 우리는 이러한 한계를 극복하기 위한 효율성 개선에 대한 연구를 시작하였다. 효율성 개선을 위해 TASSEL에서 사용후 버려지는 서열의 퀄리티를 체크하여 에러율 0.1% 이하인 데이터를 확인 한 후 퀄리티가 에러율을 충족하는 부분의 서열들을 필터링 한다. 그리고 마지막으로 바코드와 제한 효소의 부분을 확인하여 길이에 따라 서열을 잘라내어 새로운 데이터 셋으로 생성하는 구조를 반복하는 알고리즘으로 구현 하였으며, 약 17% 이상의 SNP 탐지효율성 증가함을 확인 하였다. 본 논문에서는 이와 같이 유전형 연구에서 사용되지 않는 유전체 염기서열들을 사용하여 더 많은 숫자의 단일 염기 다형성을 탐지하는 방법과 구현된 프로그램을 제시한다.

조선시대 수노리개에 나타난 표현 특성에 관한 연구 (A Study on Expressive Features of Embroidered Norigae in the Chosun Dynasty)

  • 양숙향
    • 한국지역사회생활과학회지
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    • 제22권1호
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    • pp.103-113
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    • 2011
  • The purpose of the study was to examine formative features of embroidered norigae in form, color, pattern and expressive technique through positive analysis of relics and various of collections of work and to consider expressive features of embroidered norigae. The results of the study were as follows. First, embroidered norigae has handcrafted decoration. it was made by being sewed for women longing for their family's happiness. Embroidered norigae is a dress worn by women that is hung on a coat string or the waist part of a skirt. Second, it is eco-environmental. Embroidered norigae applied things seen in nature such as flowers, butterflies and bees to its pattern. Third, it has practicality. Embroidered norigae has high practical value besides a decorative function. Needle case norigae and incense case norigae provide functions in accordance with women's wisdom and skill as well as practicality. Fourth, it is praying for good luck. Women embroidered patterns symbolizing their desires in life such as their family's happiness, wealth, many sons and a long life. Fifth, it has balance and harmony. The knot of embroidered norigae has a perfect symmetry in the front/back part and in the right/left part. And the main body and tassel are symetrical in the right/left part, which gives stability and comfortableness. Embroidered norigae is classified into knot, main body and decorative part in its form. The three kinds express their unique beauty by being harmonized together. Finally, it has a property of melody. Movement of the tassel has a property of melody shaken by the wind and movement of its wearer.

현대 가방 디자인에 나타난 술 장식의 표현유형과 미적 조형성 연구 (A study on the expression types and aesthetic formativeness of tassels and fringe in modern bag design)

  • 권기영
    • 복식문화연구
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    • 제28권4호
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    • pp.429-445
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    • 2020
  • The purpose of this study is to provide ideas for the development of bag design by analyzing the expression types and aesthetic formativeness of modern bag design using tassels and fringe given the newtro trend pursuing analog sensitivity in modern society. The methods and scope of this study are as follows. First, the related literature and internet data were reviewed in order to examine the expression types and characteristics of the bags of the West and East. Next, the definition and characteristics of tassel and fringe decoration in terms of the history of clothing were analyzed. Finally, the expression types and aesthetic formativeness of tassels and fringe were derived through the analysis of modern bag design shown in the fashion collections since 2010. The bags decorated with the tassels and fringe bring a variety of visual effects depending on the attachment position, direction and material composition, concentrating the gaze and radiating original style. In this study, the expression of tassels and fringe in modern bag design was classified into three types: surface decoration, shape variability, and object combination. Next, the aesthetic formativeness of modern bag design using tassels and fringe as decorative elements appeared through the mixture of heterogeneous materials and playfulness through imagination, exaggeration through extension of length and volume, and retro characteristics through reinterpretation of tradition. This study is expected to contribute to creative ideas of modern bag design which requires a global sense, which has been used in various cultures for a long time.

현대 패션에 나타난 민속풍(Ethnic look)에 관한 연구 (A Study on Ethnic look Expressed in Modern Fashion)

  • 정연자
    • 대한가정학회지
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    • 제31권4호
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    • pp.215-229
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    • 1993
  • As a result of making an inquiry into the ethnic look of each region appearing in present-day fashion by Asia. America and Oceania. Africa and Europe as mentioned above, its characteristics could be summarized as follows: Firstly the Asian ethnic look consists of Indian, Chinese and Japanese looks as mainstream. In terms of form, the draped form constituted its mainstream, and both the draped form and the tunic form are appearing Simultaneously in the Chinese look. And in respect to color the Indian look is using luxurious primary colors red, blue, yellow and green and other regions are making overall use of natural colors. With respect to ornament various kinds of ornaments is utilized in Indian's Sari and turban. Chines's Coolie hat, straw hat and embroidery, knotted button, and bead ornament, and Japan's Obi and Obijime, etc. Secondly in America and Oceania, costurme representing the Indian look in North America and the picture of Western pioneer, and the Peruvian, Mexican and chilean ethnic looks in South America were expressing much. Here, the form consistied a draped form as its main strem, such colors as yellow, purple, grey, etc were much utilized, and the material of costume comprised knits, cotton and the like. And the ornament consisted of hats, tassel ornaments, bead ornaments, metal chain belt, long braided hair, etc. Thirdly, the African ethnic look had an exposing form and a draped form, and such colors as black, white, yellow, brown, etc were used as the fashion color. Ornaments such as precious stone, bead ornaments, animal bones, straw metal ornament, etc were used as fashion decorations. Fourthly, Russia's Cossack look, Bulgaria's Bavshika look, spain's ethnic look cane to the fore as the European look Both the draped form and the tunic form were used simultaneously as fashion form. And grey, brown, purple, etc were much used as fashion color in the European look. Such ornaments as Cossack. Bavshka, boots, tassel ornaments were much used and paisley pattern also was used.

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