• 제목/요약/키워드: tannin content

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Comparison of Flavonoid Content and Antioxidant Activities of Peel Extracts from Gardenia jasminoides Ellis by Various Solvents (치자(Gardenia jasminoides Ellis) 과피의 용매별 추출물의 Flavonoid 함량 및 항산화 활성 비교)

  • Jin, Dong-Hyeok;Oh, Da-Young;Lee, Young-Guen;Kang, Dong-Soo;Kim, Han-Soo
    • Journal of Environmental Science International
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    • v.26 no.8
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    • pp.903-911
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    • 2017
  • The purpose of this study was to measure the bioactivity and antioxidant activity of peel from Gardenia jasminoides fructus Ellis (GJE) in Namhae, Korea, following some established methods. CM (Chloroform:Methanol, 2:1, v/v), 70% ethanol, and n-butanol extracts were collected. Flavonoid content and value as a functional food ingredient of GJE peel was investigated through assessing antioxidant [DPPH (1,1'-diphenyl-2-picrylhydrazyl), ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid)], and hydroxyl radical scavenging activities; superoxide dismutase like ability; ferrous ion-chelating capacity; and tannin content by solvent extraction. Solvent extract antioxidant activities significantly increased (p<0.05) at increasing concentrations (0.2, 0.4, 0.6 mg/mL). GJE peel extracts were less active than the positive control [ascorbic acid, BHA (butylated hydroxyanisole), and EDTA (ethylenediaminetetraacetic acid disodium salt dihydrate)]. Based on the results of this study, GJE peel could be used as a natural antioxidant source due to its high antioxidant activity and bioactive compound content.

Chemical Characteristics of the Leaves and the Seeds of Korean Dendropanax (Dendropanax morbifera Lev.) (황칠나무 잎 및 종실의 화학적 특성)

  • Kim, Hyung-Ryang;Chung, Hee-Jong
    • Applied Biological Chemistry
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    • v.43 no.1
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    • pp.63-66
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    • 2000
  • Proximate analyses of free sugars, free amino acids, fatty acids, total vitamin C and the soluble tannin content of the leaf and seed of Dendropanax morbifera Lev. were determined. Moisture content was 70.2% in leaf and 72.6% in seed, and crude protein contents were 1.2% in leaf and 6.2% in seed, and ash contents were 1.7% in leaf and 0.9% in seed. Total vitamin C and soluble tannin in leaf were 56.9% and 10.7% which were five times and eleven times higher in seed, respectively. Free sugar content in leaf was higher than that in seed, with the major free sugars consisting of sucrose, glucose and fructose. Turanose and xylose were not detected in leaf, but were detected in seed in small amounts. Unsaturated fatty acids were predominant in both of leaf and seed, but major fatty acids were quite different from each other. Low levels of free amino acids were found to consist mainly of arginine, aspartic acid and glutamic acid. The highest content of mineral elements in leaf and seed were calcium and potassium, respectively.

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Changes of Enzyme Activity, Trypsin Inhibitor, Tannin and Phytic Acid during Heat Treatment of Soybean (콩의 열처리 중 효소, 트립신 저해제, 탄닌, 피트산의 함량 변화)

  • Kim, Young-Mi;Kim, Yong-Wook
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1012-1017
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    • 1998
  • This study was performed to investigate the change of lipoxygenases and urease activities, trypsin inhibitor, tannin and phytic acid contents during heat treatment of Jinpum soybean. The lipoxygenase-1 and urease possessed their activities after heating at $60^{\circ}C$ for 100 min, but their activities disappeared rapidly at $80^{\circ}C$ and $100^{\circ}C$ for 20 and 10 min. There were no lipoxygenase-2 and -3 activities in Jinpum soybean with and without heating. Trypsin inhibitor was lost 91.9%, 78,1% and 58.6% of the activity after heat treatment at $100^{\circ}C$ for 50 min, at $80^{\circ}C$ for 100 min and at $60^{\circ}C$ for 100 min, respectively. The tannin content was increased by heat treatment. The content of and phytic acid was increase after heating at $60^{\circ}C$ for 100 min, unchanged at $80^{\circ}C$ for 100 min and decreased at $100^{\circ}C$ for 100 min.

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Studies on the Analysis of Special Components of Major Pine Needles for Searching of the New Functional Substances (I) - Analysis of Pectin, Tannin and Terpenoids - (신기능성 물질 탐색을 위한 침엽수잎의 특수성분 분석에 관한 연구 (I) - 펙틴, 탄닌, 테르페노이드의 분석 -)

  • Hwang, Byung-Ho;Lee, Hyun-Jong;Kang, Ha-Young;Liu, Shunxi;Cho, Jae-Hyun;Zhao, Julan
    • Journal of Forest and Environmental Science
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    • v.13 no.1
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    • pp.134-142
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    • 1997
  • Pectin and tannin analysis were carried out to inverstigate any available components from Pinus densiflora, P. koraiensis, P. thunbergii and P. rigida. To analyze terpenoid components, the essential oils were extracted with steam distillation method from four kinds of pine needles. The essential oil was analyzed by GC and GC-MS spectroscopy. The results were summarized as follows: Pectin content was highest in P. koraiensis with 0.40%, and tannin content was highest in P. koraiensis with 1.05. Major components of P. densiflora needles were ${\alpha}$-pinene, ${\beta}$-caryophyllene, ${\Delta}^3$-carene and phytol. ${\alpha}$-Pinene, ${\Delta}^3$-carene, ${\beta}$-caryophyllene, germacrene D, and camphene were found major components in P. koraiensis. Major components of P. thunbergii needles were ${\beta}$-pinene, ${\alpha}$-pinene, ${\beta}$-caryophyllene and germacrene D. ${\beta}$-Pinene, ${\alpha}$-piene, humulene oxide and ${\alpha}$-elemene were major components in P. rigida. Sabinene and citronellol were infrequent components in P. koraiensis, and ${\alpha}$-pinene oxide was present only in P. rigida. ${\alpha}$-Pinene, limonene, and bornylacetate well known as the main components of green air bath were found in P. densiflora and P. koraiensis.

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Chemical Properties on the Quality of Marketed Roasting Green teas (시판 덖음녹차의 품질에 따른 이화학적 특성)

  • 신미경;장미경;서은숙
    • Korean journal of food and cookery science
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    • v.11 no.4
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    • pp.356-361
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    • 1995
  • This study was conducted to know the quality of marketed green teas which were devided into high, middle and low grade according to the price. we examined the content of water, ash, total nitrogen, tannin, caffeine, chlorophyll and color value and did sensory evaluation. The results were as follows: 1) The content of water was 2.17-3.67% and content of ash was 4.50-5.17%, there were no significant difference in each grade. 2) The content of total nitrogen was 4.38% in high grade, 4.60% in middle grade and 4.68% in low grade. 3) The content of tannin was 11.09% in high grade, 14.22% in middle grade and 14.44% in low grade, middle and low grade were significantly higher than high grade. 4) The nitrogen rate to tannin (N/T) was 39.5% in high grade, 32.6% in middle grade and 32.70% in low grade, N/T rate of high grade was significantly highest than others. 5) The content of caffeine is 1.78% in high grade, 1.32% in middle grade and 0.92% in low grade, high and middle grade were significantly higher than low grade. 6) The content of total chlorophyll is 297.37 mg% in high grade, 192.89 mg% in middle grade and 204.79 mg% in low grade, chlorophyll a was 69.23 mg% in high grade, 51.99 mg% in middle grade and 63.42 mg% in low grade, and chlorophyll b was 228.47 mg% in high grade, 131.21 mg% in middle grade and 141.63 mg% in low grade. 7) Yellow value of high and middle grade were significantly higher than low grade, but blue and red value were no significant diffrence in groups. 8) In sensory evaluation, appearance, taste, odor of high grade were better than others and total score was 15.25 in high grade, 12.97 in middle grade and 9.80 in low grade. 9) Price had a positive correlation with caffeine, appearance and taste, but a negative correlation with tannine. NT rate had a negative correlation with tannine, but a positive correlation with caffeine.

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The research on antioxidative effect of Sasa quelpaertensis extractum and assessment of cytotoxicity (제주조릿대 잎 추출물의 항산화효능 및 세포독성 평가에 관한 연구)

  • Lee, Song Hee;Lee, Moo Sung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.3
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    • pp.687-693
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    • 2017
  • This research analyzed cell survival rate according to antioxidative effect and cytotoxicity using Sasa quelpaertensis and Ethanol extracts. Sasa quelpaertensis used extracts of $95^{\circ}C$ boiled-water extract and 70% ethanol liquid each. For total polyphenol content, tannin content, and flavonoid content, polyphenol content of $95^{\circ}C$ boiled-water extract was 26.6 mg/g while that of 70% ethanol extract liquid was 22.3 mg/g, which means polyphenol content was higher in $95^{\circ}C$ boiled-water extract. Tannin content was 72.1 mg/g in $95^{\circ}C$ boiled-water extract which was higher than 61.2 mg/g in 70% ethanol extract liquid. Total flavonoid content was higher in 70% ethanol extract liquid (25.4 mg/g) than in $95^{\circ}C$ boiled-water extract (17.8 mg/g). Further, the researcher measured DPPH radical elimination and ABTS radical elimination. As a result, as the concentration of each extract increased, DPPH radical elimination and ABTS radical elimination increased. In the DPPH radical elimination, L-ascorbic acid was eliminated at 500 ppm and showed no change even though the concentration increased, whereas it increased in BHT and Sasa quelpaertensis leaf extracts according to concentration. ABTS radical elimination indicated the similar phenomenon, whereas BHT showed maximum elimination at 500 ppm and decreased gradually as the concentration increased. On the other hand, it gradually increased in Sasa quelpaertensis leaf extract and showed 90% elimination at 10,000 pm, and it increased in $95^{\circ}C$ boiled-water extract. Using human skin fibroblasts (3T3) to check the cell survival rate of Sasa quelpaertensis leaves showed that cell survival rate decreased between 85.6 and 66.6% in $95^{\circ}C$ boiled-water extract. The cell survival rate was between 95.4 and 92.1% when using 70% ethanol extracts. Sasa quelpaertensis has efficacy as a natural antioxidant as well as a material hyangjang.

Studies on Feed Values of Oak(Quercus serrata Thunb.) and Pine (Pinus densiflora) Browses (졸참나무지엽과 소나무지엽의 사료가치에 관한 연구)

  • 최순호;김원호;김맹중;허삼남;이성운
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.1
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    • pp.59-68
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    • 2002
  • Chemical composition, mineral compounds, cellular constituents, digestibility for dry matter, total digestible nutrients, and tannin constituents were investigated to evaluate feed value for oak and pine browses to use effectively browses as forest by products. The results of this study were as follows : Crude protein was 6.00∼7.89% for oak browse and 5.15∼6.06% for pine browse sampled through July to October, It gradually decreased in oak browse but not in pine browse as the growth of tree was progressed stages. Crude fat content of oak and pine browse were 1.90∼2.68% and 6.30∼6.33%. Crude fiber content was 33.3∼35.2% for oak browse and 33.7∼34.8% for pine browse and they tended to increase according to the growth stage of trees. Crude ash was 3.13∼2.78% for oak browse and 2.11 ∼1.93% for pine browse, respectively. Ca, Mg, Mn and Cu content were higher in oak browse than those in pine browse, but P, K, Na, Fe, and Zn content were similar each other. Mineral contents were decreased as the growth of tree was progressed(P<.05). Acid detergent fiber content of pine and oak browse were 47.7∼52.0% and 46.1∼48.1%, and they increased as the tree grew. Neutral detergent fiber content of pine and oak browse were 64.1∼67.9% and 65.0∼66.5%. NDF content was increased according to the tree growth in pine browse but it was the same in oak browse(P<.05). in vitro digestibility of dry matter was 51.7∼48.4% for pine browse that decreased according to the tree growth(p<.05) and 53.0∼5l.4% for oak browse. Total digestible nutrients of oak browse was 50.9∼52.4% and that of pine browse was 47.7∼51.1% which decreased as the tree grew. Oak browse showed the same relative feeding value(RFV) as pine browse, and it decreased as the growth of tree was progressed(P<.05). Tannin contents was 2.96% for oak browse, 6.27% for pine browse. Tannin contents decreased when browses were dried.

A Study on the Dry Matter Yield and Nutritive Values of Wild Korean Lespedeza (Lespedeza stipulacea Maxim.)

  • Lee, Hyung Suk;Lee, In-Duk
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.3
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    • pp.396-400
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    • 2002
  • This study was conducted to evaluate the dry matter (DM) yield, quality and utilization of nutrients in Korean lespedeza (Lespedeza stipulacea Maxim.) swards as a roughage source. DM yield in various stages, chemical composition, and DM digestibility were observed, and DM intake, digestibility and utilization of nitrogen and energy by Korean native goat were determined. Experimental diets include three treatments: mixture hay (MH) 100%, MH (80%)+wild Korean lespedeza hay (LH) 20% and MH (60%)+LH (40%). As growth stage advanced, the DM yield of Korean lespedeza tended to increase significantly (p<0.05). The mean DM yield of all growing stages was observed as 7,336 kg/ha. Crude protein (CP) content of Korean lespedeza tended to decrease, but fibrous contents tended to increase when the growth stage advanced. The tannin content of Korean lespedeza was the highest at the bud stage (64.7 mg/g). However, it showed a tendency to decrease as the growth stage advanced, and thus it was the lowest at the ripe seed stage (26.8 mg/g) (p<0.05). With increasing level of LH, voluntary DM intake by Korean native goats slightly increased, but no differences were observed between diets. The digestibility of DM, cellular constituents and NDF was slightly higher in LH containing diets than that of MH 100% diet (p<0.05). There was no significant difference in apparently digested N% among all diets, but retained N% and retained N% of the absorbed were higher in MH (60%)+LH (40%) than those of other diets (p<0.05). A difference in the utilization of energy was not detected. In conclusion, Juvenile LH improved the digestibility of nutrients, presumably due to its high CP content and low fibrous compound. Especially, tannin in LH did not affect in DM intake, but increased the nitrogen utilization of Korean native goats. Accordingly, it could be suggested that Korean lespedeza has a potential to be provided as a roughage source for Korean native goats.

Changes on the Characteristics of Lindera obtusiloba BL. Leaf Teas by Manufacturing Process (제조방법에 따른 생강나무(Lindera obtusiloba BL.) 잎차의 특성변화)

  • 황경아;김광수;박찬성;신승렬
    • The Korean Journal of Food And Nutrition
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    • v.16 no.4
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    • pp.365-371
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    • 2003
  • This study was analyzed to the characteristics of the Lindera obtusiloba eaf teas by various manufacture methods. Moisture content was the highest in the tea processed by air dry method, and the lowest in the tea roasted after steaming. The contents of soluble solid was higher in the fermented tea than in the others, and the lowest in the roasted tea. The content of tannin was the higher in the roasted after steaming tea than in the others, and the lowest in the fermented tea. The content of vitamin C was the higher in the roasted tea than in the others, and the lower in the steamed tea. The mineral contents of leaf teas was orderly K, Na, Mn, Ca, Fe, Mg, and Zn. The roasted after steaming tea was estimated to the most excellent in the sensory evaluation of leaf teas.

Isolation of main component and antioxidant activities on the stem and root of Rosa rugosa (해당화 줄기 및 뿌리의 주성분 분리 및 항산화활성)

  • Park, byoung-Jae
    • Korean Journal of Plant Resources
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    • v.21 no.5
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    • pp.402-407
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    • 2008
  • Total phenol content was obtained from the stem extract of 7.8 (g/100g, D.W.) and root extract of 10.5 (g/100g, D.W.) on Rosa rugosa. S-4 and R-2 compounds were isolated from the stem and root of R. rugosa. The structure of S-4 and R-2 compounds was assigned as catechin by $^{1}H,\;^{13}C$ CNMR signal and TOF-MS. The tannin content of stem extract was 752.5 (mg/100g, D.W.) as 6.3 of gallic acid, 61.5 of epicatechin and 684.8 of catechin. The tannin content of root extract was higher about 2 times (1676.0) as 6.8 of gallic acid, 160.3 of epicatechin and 1508.5 of catechin than that of stem. It was shown that catechin was main compound in the stem and root extracts of R. rugosa. Antioxidant activities were stronger low concentration, and were not different of stem and root extracts. As the results, stem and root of R. rugosa were evaluated, as natural antioxidant resource.