• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.3
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• pp.687-693
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• 2017

This research analyzed cell survival rate according to antioxidative effect and cytotoxicity using Sasa quelpaertensis and Ethanol extracts. Sasa quelpaertensis used extracts of $95^{\circ}C$ boiled-water extract and 70% ethanol liquid each. For total polyphenol content, tannin content, and flavonoid content, polyphenol content of $95^{\circ}C$ boiled-water extract was 26.6 mg/g while that of 70% ethanol extract liquid was 22.3 mg/g, which means polyphenol content was higher in $95^{\circ}C$ boiled-water extract. Tannin content was 72.1 mg/g in $95^{\circ}C$ boiled-water extract which was higher than 61.2 mg/g in 70% ethanol extract liquid. Total flavonoid content was higher in 70% ethanol extract liquid (25.4 mg/g) than in $95^{\circ}C$ boiled-water extract (17.8 mg/g). Further, the researcher measured DPPH radical elimination and ABTS radical elimination. As a result, as the concentration of each extract increased, DPPH radical elimination and ABTS radical elimination increased. In the DPPH radical elimination, L-ascorbic acid was eliminated at 500 ppm and showed no change even though the concentration increased, whereas it increased in BHT and Sasa quelpaertensis leaf extracts according to concentration. ABTS radical elimination indicated the similar phenomenon, whereas BHT showed maximum elimination at 500 ppm and decreased gradually as the concentration increased. On the other hand, it gradually increased in Sasa quelpaertensis leaf extract and showed 90% elimination at 10,000 pm, and it increased in $95^{\circ}C$ boiled-water extract. Using human skin fibroblasts (3T3) to check the cell survival rate of Sasa quelpaertensis leaves showed that cell survival rate decreased between 85.6 and 66.6% in $95^{\circ}C$ boiled-water extract. The cell survival rate was between 95.4 and 92.1% when using 70% ethanol extracts. Sasa quelpaertensis has efficacy as a natural antioxidant as well as a material hyangjang.

• Food Science and Preservation
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• v.15 no.1
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• pp.58-65
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• 2008

The Acorn (Quercus acutissima CARRUTHERS), which contains a large quantity of tannin, should be developed as a processed food as the acorn is rich in natural antioxidants and other valuable components. Accordingly, acorn extraction conditions for polyphenol and gallic acid (both antioxidants) were investigated by response surface methodology. The content of polyphenols were determined under 16 different extraction conditions based upon a central composite design. The parameters varied over $30-70^{\circ}C$ of extraction temperature, 1-5 h of extraction time, and 5-25 mL/g of solvent ratio, Gallic acid extraction was optimal at $60-100^{\circ}C$ extraction temperature, 1-5 h of extraction time, and 5-25 mL/g of solvent ratio, Epicatechin content was highest at $56.77^{\circ}C$, 4.16 hand 22.38 mL/g. Catechin content was highest at $52.37^{\circ}C$, 2h and 23.59 mL/g. The maximum catechin content was $91.30{\mu}g/mL$. Epigallocatechin content was influenced by extraction temperature and time. The maximum epigallocatechin content was $1,066.56{\mu}g/mL$ at $61.42^{\circ}C$, 4.17h, and 9.25 mL/g. The maximum value of epicatechingallate content was $125.39{\mu}g/mL$ at $47.72^{\circ}C$, 3.04h, and 24.93mL/g. Epigallocatechingallate content was influenced principally by solvent ratio and the maximum content was $61.38{\mu}g/mL$ at $48.11^{\circ}C$, 2.96h, and 24.95mL/g. The total polyphenol content was maximal at $1,332.75{\mu}g/mL$, after extraction at $61.50^{\circ}C$, 4.24h, at 9.71mL/g. The higher the extraction temperature and the longer the extraction time, the greater the polyphenol content. Gallic acid content was highest, the maximal level was $30.51{\mu}g/mL$ after $65.84^{\circ}C$, 1.65h at 17.17 mL/g, and this was influenced principally by extraction time and solvent ratio.

• Journal of the Korean Applied Science and Technology
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• v.32 no.2
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• pp.302-311
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• 2015

To better investigate the antioxidative property of thermal treatment yam (Dioscorea batatas DECNE.) in Korea, some established methods were used. 70% Methanol, 70% ethanol and chloroform-methanol (CM, 2:1, v/v) extracts were collected. 70% Methanol extract exhibited stronger antioxidative activity evaluated by ferrous ion chelating activity, NO radical scavenging activity and ${\beta}$-carotene bleaching assays. On the contrary, CM extract was the most effective in inhibiting linoleic acid peroxidation. Yam available in Korea was also analyzed for its bioactive compounds such as lycopene, chlorophyll a, b, tannin, phytic acid and total saponin contents. Total saponin was abundant in thermal treatment, which determined to be $42.52{\pm}1.88mg/g$. Based on the results obtained from this study, thermal treatment yam could be used as natural antioxidant source due to its high antioxidant activity and bioactive compound contents.

• Journal of the Korean Applied Science and Technology
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• v.33 no.2
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• pp.342-351
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• 2016

The bioactive compound and antioxidant property of Korean yam (Dioscorea batatas DECNE.) were studied using in vitro methods. Yam available in Korea was analyzed for lycopene, chlorophyll a, b, tannin, phytic acid and total saponin contents. 70% Methanol, 70% ethanol and chloroform-methanol mixture (CM, 2:1, v/v) were used to extract yam. Then the antioxidant activity evaluated through ferrous ion chelating activity, ${\beta}$-carotene bleaching method, lipid peroxidation inhibition and nitric oxide (NO) radical scavenging activity. 70% Methanol extract showed the highest ferrous ion chelating activity and NO radical scavenging activity. And CM extract was the most effective in inhibition of linoleic acid peroxidation evaluated by ${\beta}$-carotene bleaching assay and lipid peroxidation inhibition assay. Based on the results obtained, yam is a potential active ingredient that could be applied in antioxidation as well as bio-health functional food to take a good part in prevention of human diseases and aging.

• YAKHAK HOEJI
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• v.40 no.2
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• pp.183-192
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• 1996

A chemical examination of the phenolic compounds in the leaves of Sapium japonicum(Euphorbiacesae) has led to the isolation of eleven phenolic compounds, containing five hydrolysable tannins and six flavonoids. On the basis of chemical and spectroscopic evidences, the structures of these compounds were confirmed to be gallic acid(1), 5-O-caffeoyl quinic acid(2), 1-O-galloyl-3,6-(R)-HHDP-${\beta}-_D$-glucose(corilagin)(3), 1-O-galloyl-2,4(R)-DHHDP-${\beta}-_D$-glucose(furosin)(4), 1-O-galloyl-2,4-(R)-DHHDP-3,6-(R)-HHDP-${\beta}-_D$-glucose(geraniin )(5), astragalin(6), trifolin(7), afzelin(8), quercetin(9), isoquercitrin(10) and rutin(11). Among them geraniin was the main component.

• Korean Journal of Pharmacognosy
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• v.47 no.3
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• pp.237-245
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• 2016

In present study, we conducted quantification analysis of phenolic compound (paeonol), monoterpene glycoside (paeoniflorin), and tannin ((+)-catechin in the 70% ethanol extracts of non-processed Moutan Radicis Cortex (MRC) and processed MRC by roasting using a high-performance liquid chromatography coupled with photodiode array detector. Three marker components were separated on Gemini $C_{18}$ analytical column and the column was maintained at $40^{\circ}C$ using two mobile phase system consisting of 1.0% (v/v) aqueous acetic acid and 1.0% (v/v) acetic acid in acetonitrile. The flow rate and injection volume were 1.0 mL/min and 10 mL. In non-processed MRC sample, the concentrations of three marker compounds, (+)-catechin, paeoniflorin, and paeonol were 0.20, 1.18, and 2.12%, respectively. On the other hand, the concentrations of the three compounds in processed MRC samples were 0.03-0.24, not detected-1.08, and 0.76-1.82%, respectively.

• Textile Coloration and Finishing
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• v.23 no.2
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• pp.69-75
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• 2011

A pre-treatment process has been developed for natural dyeing of wool by which the dyed materials have been imparted antimicrobial efficacy against both gram-positive and gram-negative bacteria durable up to 20 washes. In this process, wool fabrics were treated with citric acid under oxidizing condition prior to dyeing. The treated fabrics were then dyed with four different types of natural dye powders obtained from leaves of silver oak, wattle, tanner's senna and flame of forest. All the natural dyes produced yellowish brown colour on wool fabrics. The washing and light fastness properties of the fabrics subjected to pre-treatment were one grade higher compared to those of the dyed fabrics without pre-treatment. The pre-treated wool fabrics showed antimicrobial efficacy against both gram-positive bacteria (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli). The fabrics dyed without pre-treatment showed antimicrobial efficacy against gram-positive bacteria (Staphylococcus aureus) only. The durability of antimicrobial efficacy was higher in pre-treated and dyed wool fabrics compared to the dyed fabrics without pre-treatment.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.3
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• pp.221-227
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• 1993

Browse from Quercus aliena Blume, Quercus mongolica Fisch.. Quercus serrata Thunb., Quercus acutissima Carruth., Quercus variabilies Blume, and Quercus dentata Thunb. were analyzed for crude protein (CP). crude fiber(CF). neutral detergent fiber(NDF). acid detergent fiber(ADF). cellulose. lignin, in vitro dry matter digestibility(DMD), and tannin acid equivalents(TAE). According to the chemical composition and DMD. the total digestible nutrients(TDN). digestible energy(DE), and metabolizable energy(ME) were estimated. Oak browse samples were collected from May through October from 1990 to 1992 in Mt. Kyeryong. Browse from Quercus aliena, Quercus acutissima, and Quercus serrata contained higher levels of CP, DMD. TDN, DE. and ME. but lower levels of CF, NDF. ADF, and TAE as compared with other oak browse. Differences among seasons and years in the concentration of CP. NDF, and organic matter digestibility(0MD) were significantly appeared in all oak browse(P<0.05). The order of preference ratings of oak browse were Quercus aliena ) Quercus acutissima ) Quercus serrata ) Quercus mongollca ) Quercus variabilies ) Quercus dentata. As the results of this study, it could be considered that Quercus aliena and Quercus acutisslma were more valuable browse source than others.

• Korean Journal of Medicinal Crop Science
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• v.5 no.3
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• pp.217-224
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• 1997

The significant chemical components estimating the quality of green tea were compared and analyzed in the shoots of Korean native tea plants. The results are summarized as follows. The contents of total nitrogen in tea leaves were in range of $3.59{\sim}4.89%$, and the tea plants grown wildly in Bosong Daewonsa, Hwasun Ssangbongsa, Shunchun Changchun-ri, Hadong Ssanggyesa. and the cultivated tea plants in Kangjin Jangwon Sanup had higher contents of total nitrogen. The contents of tannin ranged from 12.5 to 18.3%. The contents of tannin of Kuryoi Chonunsa (18.3%), Kangjin Baekryonsa (16.7%) and Naju Bulhoisa (16.4%) were higher than those of Yangkwang Chonma-ri (12.5%), Hampyong Yongam-ri (12.7%) and Yosu Udu-ri (12.8%). The contents of caffeine were in range of $2.21{\sim}3.11%$. The contents of caffeine of Kwangju Shamae Dawon (3.11%), Kangjin Jangwon Sanup (2.94%) and Shunchun Changchun-ri(2.87%) were higher than those of Tamyang Yanggak-ri(2.21%), Yosu Udu-ri(2.23%) and Kuryoi Hwaomsa (2.23%). The contents of vit. C were in range of $167.9{\sim}223.5mg/100g$. The contents of vit. C of Changsong Oshan-ri (167.9mg/100g), Shunchun Shongkwangsa (185.6mg/100g) and Yongkwang Chonma-ri (185.8mg/100g) were lower than those of Kwangju Shamae Dawon (291.5mg/100g), Kangjin Jangwon Sanup (271.8mg/100g) and Shunchun Changchun-ri (269.5mg/100g). The contents of chlorophyll were in range of $187.4{\sim}332.7mg/100g$, and the mean contents were in range of 242.0mg/100g. The contents of total amino acids were in range of $1,888{\sim}2,500mg/100g$. The content of total amino acid at Kangjin Jangwon Sanup was the highest as 2,500mg/100g, and that of Shunchon Shongkwangsa was the lowest as 1,888mg/100g. Results of this study suggest that the native tea plants naturally grown at Bosong Daewonsa, Hwasun Ssangbongsa, Shunchun Changchun-ri, Hadong Ssanggyesa, and the cultivated tea plants at Kangjin Jangwon Sanup show excellent quality in terms of abundance of total nitrogen and total amino acids, and less contents of tannin.

• Journal of the Korean Society of Food Culture
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• v.5 no.2
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• pp.229-233
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• 1990

Changes in chemical components of green tea leaves at different cooking conditions-blanching and frying-were surveyed as a method of utilizing low graded green tea leaves which missed appropriate plucking times. There was no significant difference in protein content during cooking. But contents of chlorophyll, tannin, vitamin C and caffeine were decreased during cooking, contents of chlorophyll and caffeine showed a greater decrease in frying than blanching. Content of total amino acid of green tea leaf was 15.8% and blanching showed bigger diminution of its content in comparision with frying during cooking.