• BMB Reports
• /
• v.42 no.11
• /
• pp.764-768
• /
• 2009

The tandem ubiquitin-interacting motif (UIM) domain located at the N-terminus of Receptor Associated Protein 80 (RAP80) plays a crucial role in ionizing radiation (IR)-induced DNA damage response. RAP80 translocates to sites of IR-induced DNA damage through interaction of its UIM domain with ubiquitinated H2A and Lys63-linked polyubiquitin chains. The exact mechanism, however, through which RAP80 associates with Lys63-linked polyubiquitin chains is not clear. Here, we show by in vitro GST-pull down assays that modifying the linker region between the tandem ubiquitin binding domains of RAP80 changes the binding affinity for Lys63-linked polyubiquitin chains and affects translocation to sites of DNA breaks. Based on these findings, we suggest that the length of the linker region between the tandem ubiquitin binding domains of RAP80 may be a key factor in the binding of RAP80 with Lys63-linked polyubiquitin chains as well as in the translocation of RAP80 to DNA break sites.

• Rapid Communication in Photoscience
• /
• v.4 no.4
• /
• pp.82-85
• /
• 2015

Photoelectrochemical cell (PEC) is one of the attractive ways to produce clean and renewable energy. However, solar to hydrogen production via PEC system generally requires high external bias, because of material's innate electronic band potential relative to hydrogen reduction potential and/or charge separation issue. For spontaneous photo-water splitting, here, we design dye-sensitized solar cell (DSSC) and their monolithic tandem cell incorporated with a $BiVO_4$ photoanode. $BiVO_4$ has high conduction band edge potential and suitable band gap (2.4eV) to absorb visible light. To achieve efficient $BiVO_4$ photoanode system, electron and hole mobility should be improved, and we demonstrate a tandem cell in which $BiVO_4/WO_3$ film is connected to cobalt complex based DSSC.

• Journal of Welding and Joining
• /
• v.25 no.1
• /
• pp.57-62
• /
• 2007

In the coarse grained HAZ of conventional TiN steel, most TiN particles are dissolved and austenite grain growth easily occurrs during high heat input welding. To avoid this difficulty, thermal stability of TiN particles is improved by increasing nitrogen content in EH36-TM steel. Increased thermal stability of TiN particle is helpful for preventing austenite grain growth by the pinning effect. In this study, the mechanical properties and microstructures of high heat input welded Tandem EGW joint in EH36-TM steel with high nitrogen content were investigated. The austenite grain size in simulated HAZ of the steel at $1400^{\circ}C$ was much smaller than that of conventional TiN steel. Even for high heat input welding, the microstructure of coarse grained HAZ consisted of fine ferrite and pearlite and the mechanical properties of the joint were sufficient to meet all the requirements specified in classification rule.

• The KSFM Journal of Fluid Machinery
• /
• v.7 no.2 s.23
• /
• pp.27-34
• /
• 2004

The performance of a centrifugal compressor composed of an impeller, tandem diffuser rows and axial guide vanes has been predicted numerically and compared with available experimental results on its design rotational speed. The pitchwise-averaged mixing plane method was employed for the boundaries between rotor and stator to obtain steady state solutions. The overall characteristics showed difference according to the relative positions of tandem diffuser rows while the characteristics of impeller showed almost identical result. The numerical results agree with the measured data in respect of their tendency. It turned out that $0\%$ of relative positions is the worst case in terms of static pressure recovery and efficiency. According to the experimental results, some pressure fluctuations and malfunction of the compressor were observed for $75\%$ case. However, this numerical calculation using mixing plane method did not capture any of those phenomena. Thus, unsteady flow calculation should be performed to investigate the stability of the compressor caused by different diffuser configuration.

• Transactions of the Korean Society of Machine Tool Engineers
• /
• v.13 no.4
• /
• pp.44-55
• /
• 2004

In order to meet the requirement for higher thickness accuracy in tandem cold rolling processes, it is strongly necessary to have good performance in control units. To meet this requirement, this paper suggested an output regulating control system with a roll-eccentricity estimator for each rolling stand of tandem cold mills. Considering entry thickness variation and roll eccentricity simultaneously as the major disturbances, a synthesis of multivariable control systems was presented based on H$\infty$ control theory, which could reflect the knowledge of input direction and spectrum of disturbance signals on design. Then, to effectively reject roll eccentricity, a weight function having some poles on the imaginary axis was introduced. This lead to a non-standard H$\infty$ control problem, and the design procedures for solving this problem were analytically presented. The effectiveness of the proposed control method was evaluated through computer simulations and compared to that of the conventional linear quardratic control and feedforward control methods for roll eccentricity.

• Wind and Structures
• /
• v.30 no.5
• /
• pp.475-483
• /
• 2020

This work investigates Reynolds number Re (= 50 - 200) effects on the flows around a single cylinder and the two tandem (center-to-center spacing L^⁎= L/D = 4) cylinders, each of a diameter D. Vorticity structures, Strouhal numbers, and time-mean and fluctuating forces are presented and discussed. For the single cylinder, with increasing Re in the range examined, the vorticity magnitude, Strouhal number and fluctuating lift all monotonically rise but time-mean drag, vortex formation length, and lateral distance between the two rows of vortices all shrink. For the two tandem cylinders, the increase in Re leads to the formation of three distinct flows, namely reattachment flow (50 ≤ Re ≤ 75), transition flow (75 < Re < 100), and coshedding flow (100 ≤ Re ≤ 200). The reattachment flow at Re = 50 is steady. When Re is increased from 75 to 200, the Strouhal number of the two cylinders, jumping from 0.113 to 0.15 in the transition flow regime, swells to 0.188. The two-cylinder flow is more sensitive to Re than the single cylinder flow. Fluctuating lift is greater for the downstream cylinder than the upstream cylinder while time-mean drag is higher for the upstream cylinder than for the other. The time-mean drags of the upstream cylinder and single cylinder behaves similar to each other, both declining with increasing Re.

• Journal of the Korean Magnetic Resonance Society
• /
• v.15 no.2
• /
• pp.175-186
• /
• 2011

Amyloid fibrils have long been known to be the well known ${\alpha}$-helix to ${\beta}$-sheet transition characterizing the conversion of cellular to scrapie forms of the prion protein. A very short sequence of Yeast prion-like protein, GNNQQNY (SupN), is responsible for aggregation that induces diseases. KSI-fused tandem repeats of SupN vector are constructed and used to express SupN peptide in Escherichia coli (E.Coli). A method for a production, purification, and cleavage of tandem repeats of recombinant isotopically enriched SupN in E. coli is described. This method yields as much as 20 mg/L of isotope-enriched fusion proteins in minimal media. Synthetic SupN peptides and $^{13}C$ Gly labeled SupN peptides are studied by Congo Red staining, Birefringence and transmission electron microscopy to characterize amyloid fibril formation. To get a better understanding of aggregation-structure relationship of 7 residues of Yeast prion-like protein, the change of a conformational structure will be studied by $^{13}C$ solid-state nmr spectroscopy as powder of both amorphous and fibrillar forms.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.43 no.3 s.309
• /
• pp.54-60
• /
• 2006

This paper presents an effective algorithm for transcoding between the Enhanced Variable Rate Codec(EVRC) and G.729A. The simplest way to communicate between heterogeneous speech networks is the cascade connection of two different codecs, called tandem coding. However, tandem coding not only produces high computational loads, but also makes long delay, These problems can be solved by using the transcoding algorithm. The proposed algorithm consists of LSP (Line Spectral Pair) conversion, pitch delay conversion and algorithm for reduction of delay. Experimental results show the proposed algorithm produces lower computational complexity, shorter algorithm delay, and similar speech quality when compared with the tandem algorithm.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.115-115
• /
• 2017

Zinc finger proteins constitute a large family which has been studied to have various functions in different organisms. Tandem CCCH zinc finger proteins (TZFs), members of the zinc finger protein family, are known to participate as post-transcriptional regulators of gene expression in eukaryotes. Here, we showed that the OsZn15, a gene for tandem CCCH zinc finger protein, is induced by abiotic stress and its overexpression in transgenic rice plants (PGD1:OsZn15) gains higher drought tolerance. Gene expression analysis of promoter:GFP plants revealed that OsZn15 is specifically expressed in anther and embryo, but not in vegetative organs. In-field evaluation, grain yield was higher in the PGD1:OsZn15 than nontransgenic plants under drought conditions. Interestingly, OsZn15 is shown to not only localize at nucleus but also co-localize with both processing bodies (PB) and stress granules (SG), two messenger ribo-nucleoprotein complexes which are known to activate by forming cytoplasmic foci under stress conditions. In sum, these results suggest that OsZn15 increases drought stress tolerance of rice probably by participating in RNA turnover in PB and SG.

• Journal of Microbiology and Biotechnology
• /
• v.27 no.11
• /
• pp.2028-2036
• /
• 2017

The Escherichia coli K-12 and B strains are among the most frequently used bacterial hosts for scientific research and biotechnological applications. However, omics analyses have revealed that E. coli K-12 and B exhibit notably different genotypic and phenotypic attributes, even though they were derived from the same ancestor. In a previous study, we identified a limited number of proteins from the two strains using two-dimensional gel electrophoresis and tandem mass spectrometry (MS/MS). In this study, an in-depth analysis of the physiological behavior of the E. coli K-12 and B strains at the proteomic level was performed using six-plex isobaric tandem mass tag-based quantitative MS. Additionally, the best lysis buffer for increasing the efficiency of protein extraction was selected from three tested buffers prior to the quantitative proteomic analysis. This study identifies the largest number of proteins in the two E. coli strains reported to date and is the first to show the dynamics of these proteins. Notable differences in proteins associated with key cellular properties, including some metabolic pathways, the biosynthesis and degradation of amino acids, membrane integrity, cellular tolerance, and motility, were found between the two representative strains. Compared with previous studies, these proteomic results provide a more holistic view of the overall state of E. coli cells based on a single proteomic study and reveal significant insights into why the two strains show distinct phenotypes. Additionally, the resulting data provide in-depth information that will help fine-tune processes in the future.