• Title/Summary/Keyword: tandem

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Applications of Tandem Mass Spectrometry in the Structure Determination of Permethylated Sialic Acid-containing Oligosaccharides

  • Yoo, Eun-Sun;Yoon, In-Mo
    • Bulletin of the Korean Chemical Society
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    • v.26 no.9
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    • pp.1347-1353
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    • 2005
  • Sets of sialic acid-containing trisaccharides having different internal and terminal linkages have been synthesized to develop a sensitive method for analysis of the reducing terminal linkage positions. The trisaccharides, sialyl($\alpha$ 2-3)Gal($\beta$ 1-3)GalNAc and sialyl($\alpha$ 2-3)Gal($\beta$ 1-X)GlcNAc where X=3, 4 and 6, were synthesized and examined using electrospray ionization (ESI)-collision induced dissociation (CID) tandem mass spectrometry (MS/MS). The compounds chosen for this study are related to terminal groups likely to be found on polylactosamine-like glycoproteins and glycolipids which occur on the surface of mammalian cells. The purpose of this study is to develop tandem mass spectrometral methods to determine detailed carbohydrate structures on permethylated or partially methylated oligosaccharides for future applications on biologically active glycoconjugates and to exploit a faster method of synthesizing a series of structural isomeric oligosaccharides to be used for further mass spectrometry and instrumental analysis.

Design of Robust Speech Recognition System Using Tandem Architecture (탠덤 구조를 이용한 강인한 음성 인식 시스템 설계)

  • Yun, Young-Sun;Lee, Yun-Keun
    • Proceedings of the KSPS conference
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    • 2007.05a
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    • pp.323-326
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    • 2007
  • The various studies of combining neural network and hidden Markov models within a single system are done with expectations that it may potentially combine the advantages of both systems. With the influence of these studies, tandem approach was presented to use neural network as the classifier and hidden Markov models as the decoder. In this paper, we applied the trend information of segmental features to tandem architecture and used posterior probabilities, which are the output of neural network, as inputs of recognition system. The experiments are performed on Aurora2 database to examine the potentiality of the trend feature based tandem architecture. The proposed method shows the better results than the baseline system on very low SNR environments.

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A Computational Study on Turbulent Flows around Single and Tandem Two-Dimensional Hydrofoils with Shallow Submergence

  • Kim, H.T.;Park, J.B.;Kim, W.J.
    • Journal of Ship and Ocean Technology
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    • v.4 no.1
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    • pp.11-20
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    • 2000
  • Reynolds-averaged Navier-Stokes equations are numerically solved using a secondorder finite difference method for the analysis of turbulent flows around single and tandem hydrofoils advancing under the free surface. The location of the free surface, not known a priori, is computed from the kinematic free surface condition and the computational grid is conformed at each iteration to the free surface deformation. The eddy viscosity model of Baldwin-Lomax is employed for the turbulence closure. The method is validated through the comparision of the numerical results with the experimental data for a single hydrofoil of a Joukowski foil section. A computational study is also carried out to investigate the effect of the submergence depth and the Froude number on the lift and the drag of the hydrofoil. For tandem hydrofoils, computations are performed for several separation distances between the forward and aft foils to see the interference effect. The result shows clearly how the lift and drag change with the separation distance.

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Optimization of the tunnel Diode for GaAs/Ge Tandem Solar Cell (GaAs/Ge Tandem Solar Cell에 관한 터널 다이오드 최적화 연구)

  • Yang, S.M.;O, B.G.;Lee, M.G.;Cha, In-Su
    • Solar Energy
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    • v.18 no.1
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    • pp.35-43
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    • 1998
  • In two terminals monolithic tandem solar cells, tunnel diode is an important variable to improve conversion efficiency depending on current matching between the top and the bottom cells. Especially, the GaAs/Ge tandem is one of the most interesting cells for its high potential efficiency. This paper shows that physical analysis about I-V specific character of the GaAs/Ge solar cell, which is grown by MOCVD for GaAs or CVD for Ge, using computer simulation and experimental results, varying with thickness of the tunnel diode layer and concentration.

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Output Tracking of an Unmanned Tandem Helicopter Based On Dynamic Augment Method

  • Hu, Chun-hua;Zhu, Ji-hong;Huang, Xing-li;Hu, Jin-chun;Sun, Zeng-qi
    • International Journal of Control, Automation, and Systems
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    • v.2 no.2
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    • pp.156-164
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    • 2004
  • The unmanned tandem helicopter, which is a MIMO nonlinear system with complexity and inherent instability, exists in unstable zero dynamics. In this paper, approximate linearization is presented to design the controller for output tracking of an unmanned tandem helicopter based on the dynamic augment method, and the simulation results are encouraging.

PCR Analysis of Four Length-Polmorphic Loci in Korea Population for Genotyping

  • Ryu, Jae-Song;Koo, Yoon-Mo;So, Jae-Seong
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.3
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    • pp.169-173
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    • 2000
  • On human chromoscomes, a short sequence of DNA is known to repeat a number of times. These are called variable number of tandem repeat (VNTR) or short tandem respeat (STR) which has a short core. VNTR and STR are used in the filed of forensic science, evolution, and anthropology. In this work, we examined allele frequencies of one VNTR (YNZ22) and three STRs (NeuR, D21S11, Humth01) in a korean population sample by polymerase chain reaction (RCP) followed by high-resolution polyacrylamide gel electro-phoresis (PAGE) with silver stain. Subsequently, the polymorphism information content (PIC) was calculated : the hifhest PIC was observed in the NeuR locus (0.95680) and lowest in the Humth01 locus (0.75809).

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Noninteracting and Loop-Shaping LQ Controller Design for Tandem Cold Mills (연속 냉간압연 시스템을 위한 비간섭 루프형성 LQ제어기 설계)

  • 김종식;김철민
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.18 no.10
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    • pp.2629-2639
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    • 1994
  • A robust multivariable controller is synthesized for tandem cold mills. A blocked-noninteracting control method is applied for simplifying the structure of rolling control systems. And, a loop-shaping LQ control method is applied for maintaining the variation of the thickness and tension of each rolling stand as small as possible. In this paper, the effects of the design parameter on loop-shaping and the number of control inputs are evaluated. The simulation results show that the thickness and tension control accuracy of tandem cold mills can be improved by the blocked-noninteracting and compensated loop-shaping LQ controller.

Optimal Dynamic Operating Policies for a Tandem Queueing Service System

  • Hwang, Dong-Joon
    • Journal of the Korean Operations Research and Management Science Society
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    • v.4 no.1
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    • pp.51-67
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    • 1979
  • This paper considers the problem of determining an optimal dynamic operating policy for a two-stage tandem queueing service system in which the service facilities (or stages) can be operated at more than one service rate. At each period of the system's operation, the system manager must specify which of the available service rates is to be employed at each stage. The cost structure includes an operating cost for running each stage and a service facility profit earned when a service completion occurs at Stage 2. We assume that the system has a finite waiting capacity in front of each station and each customer requires two services which must be done in sequence, that is, customers must pass through Stage 1 and Stage 2 in that order. Processing must be in the order of arrival at each station. The objective is to minimize the total discounted expected cost in a two-stage tandem queueing service system, which we formulate as a Discrete-Time Markov Decision Process. We present analytical and numerical results that specify the form of the optimal dynamic operating policy for a two-stage tandem queueing service system.

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Tandem bioassays에 의한 한강 하상퇴적물의 환경독성 평가

  • 문성환;정진애;류성민;이순애;김재현;조화진;정대교;김기영;황인영
    • Proceedings of the Korea Society of Environmental Toocicology Conference
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    • 2002.10a
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    • pp.157-157
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    • 2002
  • 본 연구에서는 한강을 세 구간으로 구분하여 구간별 대표지점(의암호, 팔당호, 및 동호대교 정점)의 하상퇴적물을 grap으로 각각 채취한 후, Tandem Bioassays 을 적용하여 환경독성 수준을 평가하고 정점간 상호 비교하였다. Tandem Bioassays 이란 유해물질 오염에 의한 다양한 생태계 반응을 표현할 수 있는 최소한의 상호 보완적 환경독성 평가 체계를 의미한다. 본 연구에서 적용한 Tandem Bioassays 은 발광성미생물을 사용하는 Microtox 시험, 수서 물벼룩인 daphnia와 ceriodaphnia 시험, 그리고 개구리 배아를 이용하는 기형유발능 시험(최기형성 시험, FETAX)등으로 구성하였다.

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A Study on Development of Setup Model for Thickness Control in Tandem Cold Rolling Mill (연속냉간압연의 두께제어 모델 개발에 관한 연구)

  • 손준식;김일수;권욱현;최승갑;박철재;이덕만
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.10 no.5
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    • pp.96-103
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    • 2001
  • The quality requirements for thickness accuracy in cold rolling continue to become more stringent, particularly in response to exacting design specification from automotive customers. One of the major impacts from the tighter tolerance level is more unusable product on the head end and tail end of tandem mill coils when the mill is in transition to or from steady state rolling condition. A strip thickness control system for a tandem cold steel rolling mills is composed with blocked non-interacting controller and controllers for strip thickness and tension control of each rolling stands. An intelligent mathematical model included an elastic deformation of strip has been developed and applied to the field in order to predict the rolling force. The simulated results showed that the effect of elastic recovery should be included the model, even if the effect of elastic compression was not important.

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