• Title/Summary/Keyword: tagging system

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Mass production and application of activation tagged hairy root lines for functional genomic of secondary metabolism in ginseng

  • Choi, Dong-Woog;Chung, Hwa-Jee;Ko, Suk-Min;In, Dong-Soo;Song, Ji-Sook;Woo, Sung-Sick;Liu, Jang R.
    • Journal of Plant Biotechnology
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    • v.36 no.3
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    • pp.294-300
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    • 2009
  • Activation tagging that uses T-DNA vectors containing multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene is a powerful tool to determine gene function in plants. This approach has been successfully applied in screening various types of mutations and cloning the corresponding genes. We generated an activation tagged hairy root pool of ginseng (Panax ginseng C.A. Meyer) in an attempt to isolate genes involved in the biosynthetic pathway of ginsenoside (triterpene saponin), which is known as the major active ingredient of the root. Quantitative and qualitative variation of ginsenoside in activation tagged hairy root lines were profiled using LC/MS. Metabolic profiling data enabled selection of a specific hairy root line which accumulated ginsenoside at a higher level than other lines. The relative expression level of several genes of triterpene biosynthetic pathway in the selected hairy root line was determined by real time RT-PCR. Overall results suggest that the activation tagged ginseng hairy root system described in this study would be useful in isolating genes involved in a complex metabolic pathway from genetically intractable plant species by metabolic profiling.

A transcription factor "OsNAC075" is essential for salt resistance in rice (Oryza sativa L.)

  • Jung, Yu-Jin;Lee, Myung-Chul;Kang, Kwon-Kyoo
    • Journal of Plant Biotechnology
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    • v.38 no.1
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    • pp.94-104
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    • 2011
  • Salt stress is a major environmental factor influencing plant growth and development. To identify salt tolerance determinants, we systematically screened salt sensitive rice mutants by use of the Activator/Dissociation (Ac/Ds) transposon tagging system. In this study, we focused on the salt sensitive mutant line, designated SSM-1. A gene encoding a NAC transcription factor homologue was disrupted by the insertion of a Ds transposon into SSM-1 line. The OsNAC075 gene (EU541472) has 7 exons and encodes a protein (486-aa) containing the NAC domain in its N-terminal region. Sequence comparison showed that the OsNAC075 protein had a strikingly conserved region at the N-terminus, which is considered as the characteristic of the NAC protein family. OsNAC075 protein was orthologous to Arabidopsis thaliana ANAC075. Phylogenetic analysis confirmed OsNAC075 belonged to the OsNAC3 subfamily, which plays an important role in response to stress stimuli. RT-PCR analysis showed that the expression of OsNAC075 gene was rapidly and strongly induced by stresses such as NaCl, ABA and low temperature ($4^{\circ}C$). Our data suggest that OsNAC075 holds promising utility in improving salt tolerance in rice.

식물의 물질생산과 수분스트레스

  • 김준호
    • Proceedings of the Botanical Society of Korea Conference
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    • 1985.08b
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    • pp.59-71
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    • 1985
  • In rice, limited efforts have been made to identify genes by the use of insertional mutagens, especially heterologous transposons such as the maize Ac/Ds. We constructed Ac and gene trap Ds vectors and introduced them into the rice genome by Agrobacterium-mediated transformation. In this report, rice plants that contained single and simple insertions of T-DNA were analyzed in order to evaluate the gene-tagging efficiency. The 3'end of Ds was examined for putative splicing donor sites. As observed in maize, three splice donor sites were identified at the 3'end of the Ds in rice. Nearly 80% of Ds elements wered excised from the original T-DNA sites, when Ac cDNA was expressed under a CaMV 35S promoter. Repetitive ratoon culturing was performed to induce new transpositions of Ds in new plants derived from cuttings. About 30% of the plants carried at least one Ds that underwent secondary transposition in the later cultures. 8% of transposed Ds elements expressed GUS in various tissues of rice panicles. With cloned DNA adjacent to Ds, the genomic complexities of the insertion sites were examined by Southern hybridization. Half of the Ds insertion sites showed simple hybriodization patterns which could be easily utilized to locate the Ds. Our data demonstrate that the Ac/Ds mediated gene trap system could prove an excellent tool for the analysis of functions of genes in rice. We discuss genetic strategies that could be employed in a largee scale mutagenesis using a heterologous Ac/Ds family in rice.

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Application of FMOC-Cl for the Quantitative Determination of N-linked Oligosaccharides (FMOC 표식에 의한 Sugar Chain의 분석)

  • Kim, Dong-Hyun;Hwangbo, Sik;Chung, Gu-Yong
    • Korean Journal of Food Science and Technology
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    • v.29 no.4
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    • pp.630-634
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    • 1997
  • A fluorescence tagging agent, FMOC-Cl (9-fluorenylmethyl chloroformate) was used for the determination of 1-amino-oligosaccharide intermediates generated from glycoproteins by peptide-N $(N-acetyl-{\beta}-D-glucosaminyl)$ asparagine amidase (N-Glycanase, PNGase F). The derivatives were separated on an Amido 80 column by HPLC using a gradient system with 25 to 51% aqueous acetonitrile and monitored by a fluorometric detector. The detection limit of FMOC-amino-oligosaccharides was $0.05{\sim}1.5$ pmol with fluorometric detection at 278 nm.

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Semi-Supervised Answer Type Classification For Question-Answering System (질의 응답 시스템을 위한 반교사 기반의 정답 유형 분류)

  • Park, Seonyeong;Lee, Donghyeon;Kim, Yonghee;Ryu, Seonghan;Lee, Gary Geunbae
    • Annual Conference on Human and Language Technology
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    • 2013.10a
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    • pp.45-49
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    • 2013
  • 기존 연구에서는 질의 응답 시스템에서 정답 유형을 분류하기 위해 패턴 매칭 방식이나 교사 학습(Supervised Learning)을 이용했다. 패턴 매칭 방식은 질의 분석을 통해 수동으로 패턴을 구축해야 한다. 교사 학습에서는 훈련 데이터 전체에 정답 유형이 태깅(Tagging)되어야 하며, 이를 위해서는 사용자의 질의에 정답 유형을 수동으로 태깅하는 작업이 많이 필요하다. 웹을 통해 정답 유형이 태깅되지 않은 대용량의 사용자 질의 말뭉치를 구할 수 있지만, 이 데이터에는 정답 유형이 태깅되어 있지 않다. 따라서, 대용량의 사용자 질의에 비례하여, 정답 유형을 수동으로 태깅하는 작업량이 증가한다. 앞서 언급한 두 가지 방법론에서, 정답 유형 분류를 위해 수작업이 많이 필요하다는 문제점을 해결하고자 본 논문에서는 일부 태깅된 훈련 데이터를 필요로 하는 반교사 학습(Semi-supervised Learning)에 기반한 정답 유형 분류를 제안한다. 이는 정답 유형 분류 작업에 필요한 노동력을 최소화함으로 대용량의 데이터를 통한 효율적 질의 응답 시스템 구축을 가능하게 한다.

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Implementation Tagging System of Korea Homonym (한국어 동형이의어 태깅 시스템 구현)

  • Kim, Jun-Su;Choe, Ho-Seop;Lee, Wang-Woo;Ock, Cheol-Young
    • Annual Conference on Human and Language Technology
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    • 2002.10e
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    • pp.24-30
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    • 2002
  • 본 논문은 한국어 정보처리에서 발생하는 단어 중의성 문제를 해결하기 위하여, 사전 뜻풀이 말뭉치에서 추출하여 구축한 의미정보 데이터베이스(Semantic Information Database)와 이를 활용한 단어 중의성 해결 모델을 이용한 실용적인 동형이의어 태깅 시스템 개발을 목적으로 한다. 중 소규모의 국어사전 150,000여 개의 표제어 전체의 뜻풀이에 품사 태그를 부착한 117만 어절 규모의 뜻풀이 말뭉치를 구축한 후 사전에 등재된 14,000여 개의 동형이의어 중 뜻풀이에 나타나는 8,164개의 동형이의어에 표제어 어깨번호를 이용한 의미 태그를 부착함으로써, 대량의 동형이의어 분별을 위한 공기(coocurrence)하는 단어와 빈도(frequency)정보를 추출하여 데이터베이스화 할 수 있었다. 본 동형이의어 태깅 시스템의 정확률 측정과 문제점 발견을 위해 (21세기 세종 계획) 프로젝트에서 제공하는 150만 어절 의미 태그 부착 말뭉치를 대상으로 실험한 결과 세종 150만 의미 태그 부착 말뭉치에 고빈도로 출현하는 469개 어휘(총 출현 횟수 249.249개)에 대한 정확률이 91.58%로 나타났다.

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An Analysis of the Foxonomy Constructed at Research Information Service and Future Perspectives (학술정보서비스의 폭소노미 분석 연구)

  • Cho, Ja-Ne
    • Journal of the Korean Society for Library and Information Science
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    • v.42 no.4
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    • pp.95-112
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    • 2008
  • In contrast to traditional taxonomy, folksonomy is generated not only by experts but also by creators and consumers of the content. Folksonomy is the practice and method of collaboratively creating and managing tags to annotate and categorize content. It is also known as collaborative tagging or social indexing. Folksonomy is also used to link to create social network that connect people to people who share same interest. Folksonomy users can generally discover the contents by which the tag sets of another user who tends to interpret contents in a way that makes sense to them. Firstly, this study consider the significance and some critical issues about folksonomy. Secondly, analyze special features of Korean academic site's folksonomy, which is managed by academic information site. Accordingly consider the directions of development about folksonomy system.

Context Centrality in Distributions of Advertising Messages and Online Consumer Behavior

  • CHAE, Myoung-Jin
    • Journal of Distribution Science
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    • v.20 no.8
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    • pp.123-133
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    • 2022
  • Purpose: As moment-based marketing messages (i.e., messages related to current moments or event), companies put significant investments to distribute TV advertising related to external moments in a retail environment. While the literature offers strong support for the value of distributions of context-based messaging to advertisers, less attention has been given to how to design those messages to effectively communicate across channels. This research adds a new dimension of analysis to the study of advertising context and its cross-channel effects on online consumer behavior. Research Design, Data and Methodology: A system-of-equations Tobit regression model was adopted using data collected from an advertising agency that consists of 1,223 TV ads aired during the Rio Olympics and NCAA, tagging from consumers, and a text analysis. Results: First, TV ads with high centrality of context lead to lower online search behavior and higher online social actions. Second, how brands can design messages more effectively was explored by using product information as a moderator that could improve the impact of context-based TV advertisements. Conclusions: Given that expenses in traditional channels are still one of the biggest channel management decisions, it is critical to understand how consumer engagement varies by design of context-based TV advertising.

A Folksonomy Ranking Framework: A Semantic Graph-based Approach (폭소노미 사이트를 위한 랭킹 프레임워크 설계: 시맨틱 그래프기반 접근)

  • Park, Hyun-Jung;Rho, Sang-Kyu
    • Asia pacific journal of information systems
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    • v.21 no.2
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    • pp.89-116
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    • 2011
  • In collaborative tagging systems such as Delicious.com and Flickr.com, users assign keywords or tags to their uploaded resources, such as bookmarks and pictures, for their future use or sharing purposes. The collection of resources and tags generated by a user is called a personomy, and the collection of all personomies constitutes the folksonomy. The most significant need of the folksonomy users Is to efficiently find useful resources or experts on specific topics. An excellent ranking algorithm would assign higher ranking to more useful resources or experts. What resources are considered useful In a folksonomic system? Does a standard superior to frequency or freshness exist? The resource recommended by more users with mere expertise should be worthy of attention. This ranking paradigm can be implemented through a graph-based ranking algorithm. Two well-known representatives of such a paradigm are Page Rank by Google and HITS(Hypertext Induced Topic Selection) by Kleinberg. Both Page Rank and HITS assign a higher evaluation score to pages linked to more higher-scored pages. HITS differs from PageRank in that it utilizes two kinds of scores: authority and hub scores. The ranking objects of these pages are limited to Web pages, whereas the ranking objects of a folksonomic system are somewhat heterogeneous(i.e., users, resources, and tags). Therefore, uniform application of the voting notion of PageRank and HITS based on the links to a folksonomy would be unreasonable, In a folksonomic system, each link corresponding to a property can have an opposite direction, depending on whether the property is an active or a passive voice. The current research stems from the Idea that a graph-based ranking algorithm could be applied to the folksonomic system using the concept of mutual Interactions between entitles, rather than the voting notion of PageRank or HITS. The concept of mutual interactions, proposed for ranking the Semantic Web resources, enables the calculation of importance scores of various resources unaffected by link directions. The weights of a property representing the mutual interaction between classes are assigned depending on the relative significance of the property to the resource importance of each class. This class-oriented approach is based on the fact that, in the Semantic Web, there are many heterogeneous classes; thus, applying a different appraisal standard for each class is more reasonable. This is similar to the evaluation method of humans, where different items are assigned specific weights, which are then summed up to determine the weighted average. We can check for missing properties more easily with this approach than with other predicate-oriented approaches. A user of a tagging system usually assigns more than one tags to the same resource, and there can be more than one tags with the same subjectivity and objectivity. In the case that many users assign similar tags to the same resource, grading the users differently depending on the assignment order becomes necessary. This idea comes from the studies in psychology wherein expertise involves the ability to select the most relevant information for achieving a goal. An expert should be someone who not only has a large collection of documents annotated with a particular tag, but also tends to add documents of high quality to his/her collections. Such documents are identified by the number, as well as the expertise, of users who have the same documents in their collections. In other words, there is a relationship of mutual reinforcement between the expertise of a user and the quality of a document. In addition, there is a need to rank entities related more closely to a certain entity. Considering the property of social media that ensures the popularity of a topic is temporary, recent data should have more weight than old data. We propose a comprehensive folksonomy ranking framework in which all these considerations are dealt with and that can be easily customized to each folksonomy site for ranking purposes. To examine the validity of our ranking algorithm and show the mechanism of adjusting property, time, and expertise weights, we first use a dataset designed for analyzing the effect of each ranking factor independently. We then show the ranking results of a real folksonomy site, with the ranking factors combined. Because the ground truth of a given dataset is not known when it comes to ranking, we inject simulated data whose ranking results can be predicted into the real dataset and compare the ranking results of our algorithm with that of a previous HITS-based algorithm. Our semantic ranking algorithm based on the concept of mutual interaction seems to be preferable to the HITS-based algorithm as a flexible folksonomy ranking framework. Some concrete points of difference are as follows. First, with the time concept applied to the property weights, our algorithm shows superior performance in lowering the scores of older data and raising the scores of newer data. Second, applying the time concept to the expertise weights, as well as to the property weights, our algorithm controls the conflicting influence of expertise weights and enhances overall consistency of time-valued ranking. The expertise weights of the previous study can act as an obstacle to the time-valued ranking because the number of followers increases as time goes on. Third, many new properties and classes can be included in our framework. The previous HITS-based algorithm, based on the voting notion, loses ground in the situation where the domain consists of more than two classes, or where other important properties, such as "sent through twitter" or "registered as a friend," are added to the domain. Forth, there is a big difference in the calculation time and memory use between the two kinds of algorithms. While the matrix multiplication of two matrices, has to be executed twice for the previous HITS-based algorithm, this is unnecessary with our algorithm. In our ranking framework, various folksonomy ranking policies can be expressed with the ranking factors combined and our approach can work, even if the folksonomy site is not implemented with Semantic Web languages. Above all, the time weight proposed in this paper will be applicable to various domains, including social media, where time value is considered important.

A Lifelog Management System Based on the Relational Data Model and its Applications (관계 데이터 모델 기반 라이프로그 관리 시스템과 그 응용)

  • Song, In-Chul;Lee, Yu-Won;Kim, Hyeon-Gyu;Kim, Hang-Kyu;Haam, Deok-Min;Kim, Myoung-Ho
    • Journal of KIISE:Computing Practices and Letters
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    • v.15 no.9
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    • pp.637-648
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    • 2009
  • As the cost of disks decreases, PCs are soon expected to be equipped with a disk of 1TB or more. Assuming that a single person generates 1GB of data per month, 1TB is enough to store data for the entire lifetime of a person. This has lead to the growth of researches on lifelog management, which manages what people see and listen to in everyday life. Although many different lifelog management systems have been proposed, including those based on the relational data model, based on ontology, and based on file systems, they have all advantages and disadvantages: Those based on the relational data model provide good query processing performance but they do not support complex queries properly; Those based on ontology handle more complex queries but their performances are not satisfactory: Those based on file systems support only keyword queries. Moreover, these systems are lack of support for lifelog group management and do not provide a convenient user interface for modifying and adding tags (metadata) to lifelogs for effective lifelog search. To address these problems, we propose a lifelog management system based on the relational data model. The proposed system models lifelogs by using the relational data model and transforms queries on lifelogs into SQL statements, which results in good query processing performance. It also supports a simplified relationship query that finds a lifelog based on other lifelogs directly related to it, to overcome the disadvantage of not supporting complex queries properly. In addition, the proposed system supports for the management of lifelog groups by providing ways to create, edit, search, play, and share them. Finally, it is equipped with a tagging tool that helps the user to modify and add tags conveniently through the ion of various tags. This paper describes the design and implementation of the proposed system and its various applications.