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http://dx.doi.org/10.5010/JPB.2011.38.1.094

A transcription factor "OsNAC075" is essential for salt resistance in rice (Oryza sativa L.)  

Jung, Yu-Jin (Institute of Genetic Engineering, Hankyong National University)
Lee, Myung-Chul (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA)
Kang, Kwon-Kyoo (Institute of Genetic Engineering, Hankyong National University)
Publication Information
Journal of Plant Biotechnology / v.38, no.1, 2011 , pp. 94-104 More about this Journal
Abstract
Salt stress is a major environmental factor influencing plant growth and development. To identify salt tolerance determinants, we systematically screened salt sensitive rice mutants by use of the Activator/Dissociation (Ac/Ds) transposon tagging system. In this study, we focused on the salt sensitive mutant line, designated SSM-1. A gene encoding a NAC transcription factor homologue was disrupted by the insertion of a Ds transposon into SSM-1 line. The OsNAC075 gene (EU541472) has 7 exons and encodes a protein (486-aa) containing the NAC domain in its N-terminal region. Sequence comparison showed that the OsNAC075 protein had a strikingly conserved region at the N-terminus, which is considered as the characteristic of the NAC protein family. OsNAC075 protein was orthologous to Arabidopsis thaliana ANAC075. Phylogenetic analysis confirmed OsNAC075 belonged to the OsNAC3 subfamily, which plays an important role in response to stress stimuli. RT-PCR analysis showed that the expression of OsNAC075 gene was rapidly and strongly induced by stresses such as NaCl, ABA and low temperature ($4^{\circ}C$). Our data suggest that OsNAC075 holds promising utility in improving salt tolerance in rice.
Keywords
Ds insertion mutant; OsNAC075; rice; salt stress; transcription factor;
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