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Evaluation of the $HApS^{TM}$ Method for the Enumeration of Aerobic Microorganisms and Coliforms in Retailed Meat Samples in Korea

  • Keun-Seok Seo;Wonki Bae;So-Hyun Kim;Nam-Hoon Kwon;Ji-Yeun Kim
    • Journal of Food Hygiene and Safety
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    • v.16 no.3
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    • pp.194-199
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    • 2001
  • This study was designed to compare the effectiveness and applicability of the HApS$^{TM}$ (Hazard Analysis process System; HUKO, Seoul, Korea) based on Petrifilm$^{TM}$ (3M, St. Paul, MN, USA) with the AOAC (the Association of Official Analytical Chemists) standard total aerobic count (TAC) method and coliform count (CC) method for meat products. The comparisons were carried out using 230 meat samples collected from various retailers: 80 pork samples, 80 chicken samples, and 70 beef samples. In the comparison of the correlation coefficient (r) between conventional method and HApS$^{TM}$ method by a linear regression analysis, the correlation coefficients in total microorganism were 0.97767, 0.90712, and 0.95594 in pork, beef, and chicken samples, respectively. The correlation coefficients in coliform count were 0.82062, 0.94833, and 0.96839 in pork, beef and chicken samples, respectively. All the independent t-test on measurement values between conventional method and HApS$^{TM}$ method represented no significant differences in the means between two methods at the 0.05 of significance level($\alpha$=0.05). Based on the high correlation between HApS$^{TM}$ and the AOAC standard methods in the TAC and CC, it might be compatible to employ the HApS$^{TM}$ method to measure the microbial contamination in livestock products. HApS$^{TM}$ method was simpler and less time-consuming in sample preparation and procedures faster than the conventional method. These results suggested that the HApS$^{TM}$ method could be substitute for the conventional methods in the analysis of microbial contamination measurement in meat products.n meat products.

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A study on the comparison of antioxidant effects between hot pepper extract and capsaicin (고추와 Capsaicin의 항산화 효능 비교)

  • Gang, Hyeon-Min;Park, Hee-Soo;Rhim, Tae-Jin;Kwon, Ki-Rok
    • Journal of Pharmacopuncture
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    • v.11 no.1
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    • pp.109-118
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    • 2008
  • Objective : The objective of this study was to compare the antioxidant effects of hot pepper extract and capsaicin. Methods : In vitro, antioxidant activities were examined by DPPH radical scavenging activity, total antioxidant capacity(TAC), oxygen radical scavenging capacity(ORAC), inhibition of induced lipid peroxidation using liver mitochonria and total phenolic contents. Results : 1. DPPH free radical scavenging activities at the concentrations of both 1 and $10mg/m{\ell}$ were 1.2 to 1.9 times higher in capsaicin than in hot pepper extract. The concentration of capsaicin required for 50% radical scavenging was lower than that of hot pepper extract(3.9 vs $5.9mg/m{\ell}$), indicating that capsaicin had higher DPPH radical scavenging activity than hot pepper extract. 2. Total antioxidant capacities of capsaicin at the concentrations of 0.1 and 1mg/ml(13.8 and 41.3 nmol Trolox equivalent) were not significantly different from those at the concentrations of 1 and $10mg/m{\ell}$(11.4 and 41.2nmol Trolox equivalent), indicating that capsaicin showed 10 times higher ABTS radical scavenging activity compared to hot pepper extract. 3. ORAC of capsaicin at the concentrations of 1, 5, 10 and 100 mg/ml were 0.04, 0.17, 0.29 and 1.74nmol gallic acid equivalent, respectively. On the other hand, ORAC of hot pepper extract at the concentrations of 1, 5, 10 and $100{\mu}g/m{\ell}$ were 0.15, 0.44, 0.75 and 2.49nmol gallic acid equivalent, respectively, indicating that capsaicin showed higher peroxyl radical scavenging activity than hot pepper extract. 4. Inhibition of lipid peroxidation caused by hot pepper extract at the concentrations of 1 and $10mg/m{\ell}$ were 12.2 and 61.4%, respectively. Inhibition of lipid peroxidation caused by capsaicin at the concentrations of 1 and $10mg/m{\ell}l$ were 64.0 and 96.8%, respectively. Thus capsaicin showed 10 times stronger effect in inhibiton of lipid peroxidation than hot pepper extract. 5. Total phenolic contents of hot pepper extract at the concentrations of 0.1 and $1mg/m{\ell}$ were 1.4 and 20.8nmol gallic acid equivalent, respectively. Total phenolic contents of capsaicin at the concentrations of 0.1 and $1mg/m{\ell}$ were 6.1 and 55.4 nmol gallic acid equivalent, respectively, indicating that capsaicin had 2.7 to 4.3 times higher total phenolic contents than hot pepper extract. Conclusions : In summary, the results of this study demonstrate significant antioxidant activity of hot pepper extract, although the activity was lowered compared to capsaicin, suggesting that hot pepper extract play a role in prevention of oxidative-related diseases.

Comparison of Chemical Composition and Radical Scavenging Activity of Pomegranate Extracts from Different Growing Areas

  • Lee, Hyun-Jung;Cho, Hye-Jin;Lee, Hyun-Sun;Kwon, Ki-Han;Hong, Yang-Hee;Kim, Seon-Hee;Suh, Hyung-Joo
    • Preventive Nutrition and Food Science
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    • v.14 no.3
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    • pp.214-219
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    • 2009
  • The pomegranate (Punica granatum L.) is a promising source of functional food, which contains several phytochemicals that perform important roles in reducing the risk of pathological diseases. Chemical compositions, such as the total sugar, uronic acid, polyphenols, and anthocyanin contents, and radical scavenging activity were determined and compared among PEs from six different cultivation areas. Total anthocyanin contents (TAC) and total polyphenol contents (TPC) from various growing areas were detected in the following order, respectively: Spain (19.08 ${\mu}g$/mL)> Turkey (12.91 ${\mu}g$/mL)> Iran-A (6.67 ${\mu}g$/mL)> Taiwan (4.77 ${\mu}g$/mL)> Uzbekistan (1.88 ${\mu}g$/mL)> Iran-B (0.76 ${\mu}g$/mL) and Turkey (639.52 ${\mu}g$/mL)> Uzbekistan (502.19 ${\mu}g$/mL)> Spain (306.40 ${\mu}g$/mL)> Iran-B (249.20 ${\mu}g$/mL)> Taiwan (162.78 ${\mu}g$/mL)> Iran-A (143.93 ${\mu}g$/mL). PEs from six different cultivation areas were determined to vary in ellagic acid content from 8.90 ${\mu}g$/mL to 332.52 ${\mu}g$/mL. The amounts of total sugars in PE from Iran-A evidenced relatively high total sugar contents, but low uronic acid levels (11.92 mg/mL). DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activities were as follows: Turkey> Uzbekistan $\gg$ Spain> Iran-B> Iran-A> Taiwan. ABTS [2,20-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] radical scavenging activities were detected in the following order: Turkey$\geq$ Uzbekistan$\gg$ Spain> Iran-B> Iran-A> Taiwan. These results indicate that the PEs from Turkey with higher levels of TPC, TAC, ellagic acid, and higher radical scavenging activity may constitute a promising functional source for the prevention of several degenerative diseases.

On Institutional Arrangements of Quota-Based Management for China's Marine Capture Fisheries (쿼터제에 기초한 중국연근해 어업관리에 대한 제도적 연구)

  • Mu, Yong-Tong;Choe, Jeong-Yoon
    • The Journal of Fisheries Business Administration
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    • v.32 no.2
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    • pp.91-125
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    • 2001
  • 이 논문은 2002년도 부경대학교 수산경영학과 박사학위논문(A Study on Institutional Arrangements for Quota-Based Management : The Case of China's Marine Capture Fisheries)의 일부이다. 이 논문의 기본목적은 취터제에 기초한 중국의 어업관리를 위한 제도적인 틀을 세우고자 하는데 있으며, 이런 목적을 달성하기에 위해 이 논문은 다음과 같이 구성하여 연구를 진행시켰다. 제1장은 연구목적과 문제의 제기에 이어 제2장에서는 중국 해양어업의 개발과 이에 따른 문제점을 분석하였으며, 여기에서 궈터제에 기초한 어업관리정책의 제도적 제안을 함과 동시에, 제3장에서는 중국 해양어업의 현실과 중국 사회의 일반적인 현상을 분석하여 제4장을 통해 쿼터제에 기초한 중국의 어업관리를 위한 제도적 프레임러크를 제안하였다. 마지막으로 본 논문을 요약하고 향후 계속되어야 할 연구과제를 제시하였다. 본 논문의 중요한 연구 결과는 다음과 같다. 현재 중국 수산업의 관리체제는 질적 규제 중심 관리제도, 그리고 정부 주도하의 어업관리 과정으로 특징지을 수 있다. 이러한 명령과 통제 (command-and-control) 방식의 전통적인 중국어업 관리제도로서는 어업인들에게 있어서 자원 보존에 대한 동기와 제도에 대한 수용성을 약화시키며, 정부 규제를 오히려 무시하게 되는 결과를 초래한다. 결과적으로 현형 중국의 어업관리제도는 어업인에게 있어서 자원 보전에 대한 유인을 약화시키고 단기적인 수익을 극대화하도록 유도하는 맹점을 지니므로 인해 더 이상 지속적인 어업성립의 보장을 할 수 없다는 점을 지적할 수 있다. 중국정부는 이러한 어업관리제도의 불완전성을 인식하여 2000년 10월 31일에 1986년 에 제정된 수산업법을 개정하게 되었으며, 새로운 중국의 수산업법은 어업 허가제도와 함께 할당량제에 기초한 어업관리제도를 국가가 운영하는 것으로 특징지을 수 있다. 그러나 아직까지 중국은 할당제에 근거한 어업관리제도를 미실시중에 있으며, 그 내용도 애매모호한 점을 많이 가지고 있다. 또한 중국의 절대적 어획량은 현재 논란의 여지로 남아있고, 거대 어업 인구의 근본적인 요구와 중국 어선의 국제적인 경쟁력, 그리고 유어(遊漁)에 대한 사회적 요구 등도 중국의 수산정책이 채결해야 할 과제이다. 위에서 고찰한 내용을 기초로 하여 우리는 중국의 쿼터제에 기초한 어업관리에 대해 다음과 같은 몇 가지를 제안한다. 중국 연근해어업에 있어서 할당제 모형에 참가하는 어업 참여자들의 유형은 세 가지로 구분할 수 있다. 첫째는 어촌에 거주하면서 생존수단으로 소규모 어업에 종사하는 경우이고, 둘째는 유어어업의 경우이며, 셋째는 상업적 목적으로 어업에 종사하는 집단으로 나눌 수 있다. 이러한 세 집단을 하나의 제도의 틀로 통합하기 위해서는 국가의 총허용어획량(TAC)을 총 어촌 허용어획량(TAIVCs)과 총 허용 유어 어획량(TARCs) 및 총허용 상업적 어획량 (TACCs)의 세부문으로 나눌 것을 제안한다. 그리하여 다시 TAFVCs와 TARCs, 그리고 TACCs는 어촌별 어획량(ITCQs), 개별 유어 회사별 어획량(IRCQs), 그리고 양도 가능한 개별 상업적 어획량(ITCQs)으로 나누어 관리하도록 한다. 이와 같이 중국의 국가 총허용어획량(TAC)을 IFVQs와 IRCQs, 그리고 ITQCQs의 세부문으로 구분하여 할당하고, 여기에 기초하여 어업을 관리하는 것은 중국에 있어서 사회적 형평성과 경제적 효율성과의 상충관계를 고려하여 고안된 것으로, 중국에 있어서 수산업을 통한 경제적 및 사회적 효과를 동시에 기대할 수 있을 것이다.

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Analysis of the Outdoor Design Conditions for Greenhouse Heating and Cooling Systems in Korea (온실의 냉난방시스템 설계용 외부기상조건 분석)

  • Nam, Sang-Woon;Shin, Hyun-Ho
    • Journal of Bio-Environment Control
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    • v.25 no.4
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    • pp.308-319
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    • 2016
  • In order to set the outdoor weather conditions to be applied to the design standard of the greenhouse heating and cooling system, outdoor air temperature and heating degree-hour for heating design, dry bulb temperature, wet bulb temperature and solar irradiance for cooling design were analyzed and presented. For every region in Korea, we used thirty years from 1981 to 2010 hourly weather data for analysis, which is the current standard of climatological normal provided by KMA. Since the use of standard weather data is limited, design weather conditions were obtained using the entire weather data for 30 years, and the average value of the entire data period was presented as a design standard. The design weather data with exceedance probability of 1, 2.5, and 5% were analyzed by the TAC method, and we presented the distribution map with exceedance probability of 1% for heating and 2.5% for cooling which are recommended by design standards. The changes of maximum heating load, seasonal heating load and maximum cooling load were examined by regions, exceedance probabilities, and setpoint temperatures. The proposed outdoor design conditions can be used not only directly for the greenhouse heating and cooling design, but also for the reinforcement of heating and cooling facilities and the establishment of energy saving measures. Recently, due to the climate change, sweltering heat in summer and abnormal temperature in winter are occurring frequently, so we need to analyze weather data periodically and revise the design standard at least every 10 years cycle.

알루미늄 양극산화 피막의 색조에 미치는 전해 인자의 영향

  • Choe, In-Cheol;Jo, Hyo-Jae;Son, In-Jun
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2017.05a
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    • pp.131-131
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    • 2017
  • 알루미늄은 내식성, 내마모성과 같은 물리적, 화학적 성질이 우수하지 못하여 이를 향상시키기 위해서 양극산화법이 산업적으로 널리 사용되고 있다. 알루미늄 양극산화법을 적용하면 강도, 내마모성 및 내식성이 향상될 뿐만 아니라 알루미늄 표면에 규칙적으로 배열된 30nm~100nm 크기의 pore에 염료를 흡착시켜 다양한 색상의 외관을 가지는 양극산화피막을 형성시킬 수 있다. Pore간의 간격은 수십nm~수백nm 정도이며, pore의 크기와 간격 및 깊이는 양극산화조건(양극산화 전압, 전해액의 종류와 농도 및 온도)에 의해 크게 변화된다. 또한 염료의 농도와 착색 시간에 따라서 양극산화 피막의 색조가 변화되는 것으로 알려져 있다. 따라서 본 연구에서는 양극산화피막의 색조에 미치는 전해조건의 영향을 조사하고, 분광측색계를 사용하여 산화피막의 색조를 정량적으로 분석하고 또한 산화피막의 pore에 흡착된 염료를 정량분석하기 위해서 UV-visible을 사용하여 분석하였다. Al5052 합금을 이용하여 에칭, 양극산화, 착색처리, 봉공(sealing)처리를 실시하였다. $55^{\circ}C$ 100g/L NaOH 용액에서 에칭을, $25^{\circ}C$ 10 vol.% $HNO_3$ 용액에서 디스머트를 실시한 다음, $25^{\circ}C$ 10 vol.% $H_2(SO_4)$ 용액에서 15V의 정전압으로 양극산화를 실시하였다. 이후, $55^{\circ}C$ 5~8g/L의 오렌지, 블랙 착색염료(일본 OKUNO 사(社)의 TAC-LH(301), TAC-BLH(411))를 사용하여 착색처리를, $85^{\circ}C$ 초산니켈 수용액에서 봉공처리를 실시하였다. 착색조건으로는 양극산화 시간(5분, 10분, 15분, 20분), 착색 시간(15초, 1분, 2분, 5분) 및 착색 농도(오렌지 -2.5g/L, 5g/L, 7.5g/L, 블랙 - 4g/L, 8g/L, 12g/L)를 변화시켰으며, 산화피막의 색조를 정량 분석하기 위해 분광측색계를 사용하였고 흡착된 염료의 농도를 정량 분석하기 위해서 $55^{\circ}C$, 1M NaOH에 재용해하여 UV-visible로 흡광도를 측정하였다. 양극산화피막의 색조는 양극산화 시간이 길어질수록, 착색시간이 길어질수록, 착색농도가 진할수록 산화피막에 흡착되는 염료의 양이 증가하며 색조가 더 선명해지고 진해지는 것을 확인하였다. 이를 분광측색계로 분석하였을 때 각 전해조건하에서 경향성을 나타내었다. 또한 흡광도 측정을 통해 계산한 염료의 양과 전해조건의 상관관계를 조사하였다. 양극산화 시간이 길어지면 산화피막의 두께가 증가하여 염료가 흡착될 수 있는 표면적이 넓어지고, 착색 시간이 길어지면 동일한 산화피막에 더 많은 염료가 흡착이 된다. 그리고 착색 농도가 진할수록 동일면적, 동일시간 하에서 더 많은 염료가 흡착되어 결과적으로 전해조건이 강해질수록 산화피막의 색조가 진해지는 것으로 판단된다.

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Regulation of Corynebacterium ammoniagenes purF and Isolation of purF-Specific Regulatory Proteins (Corynebacterium ammoniagenes에서 purF 유전자의 조절 및 이에 특이적인 조절 단백질의 분리)

  • Lee, Seok-Myung;Kim, Youn-Hee;Lee, Heung-Shick
    • Korean Journal of Microbiology
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    • v.45 no.3
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    • pp.233-238
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    • 2009
  • The expression of Corynebacterium ammoniagenes purF was analyzed by utilizing a plasmid carrying a cat gene fused to the purF promoter region. Adenine and guanine repressed the expression of the purF gene by 20~30% but hypoxanthine did not exert such repressive effect. The expression purF was maximal at the late log phase and remained constant throughout the stationary phase. Promoter $P_{180}$ which was developed in C. glutamicum was also functional in C. ammoniagenes, achieving maximal activity at the late log phase. The promoter outperformed Escherichia coli $P_{tac}$ promoter by 40~50% level. DNA-affinity purification identified a protein which could bind to the promoter region of the purF gene. The protein showed high similarity to the CRP-family transcriptional regulator encoded by NCgl0120 in C. glutamicum. The size of the screened protein agreed with the expected protein size from the ORF NCgl0120. The corresponding gene in C. ammoniagenes encoded a 42 kDa polypeptide composed of 400 amino acids with expected pI of 4.9. The encoded protein showed 14.1% and 15.8% identity with E. coli and Bacillus subtilis PurR, respectively, suggesting that the isolated protein might be a novel type of regulatory protein involved in the regulation of purine metabolism.

Thermal Behavior and Abrasion Properties of Glass Fiber Reinforced Nylon 12 Crosslinked by Electron Beam Irradiation (전자선 가교된 유리섬유 강화 나일론 12의 열적 거동 및 내마모 특성)

  • Shin, Bum-Sik;Jeun, Joon-Pyo;Kim, Hyun-Bin;Kang, Phil-Hyun
    • Polymer(Korea)
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    • v.35 no.1
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    • pp.30-34
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    • 2011
  • In this study, the effects of the electron beam irradiation on the thermal behavior and the abrasion properties of the glass fiber reinforced nylon 12 was investigated. The electron beam irradiation was carried out over a range of irradiation dose from 100 to 600 kGy with additive crosslinking agents such as triallyl cyanurate (TAC), triallyl isocyanurate (TAIC) and trimethylolpropane trimethacrylate (TMPTMA) for enhancing the crosslinking effects. The gel contents were increased dramatically above 200 kGy. It was verified that the degree of crosslinking depends on the radiation dose. The decreases of the melting temperature and the area of endothermic peak were observed as increasing the absorbed dose in the results of DSC analysis. The enhanced thermal stability was confirmed by the increases of decomposition temperature by electron beam irradiation. Furthermore, the negative deviations of the abrasion loss and the abrasion coefficients confirmed the improvement of the abrasion properties of irradiated nylon 12, as evidenced by SEM observation on the abrasion surfaces. The addition of the crosslinking agents to nylon 12 during effectively improved the thermal behavior and the abrasion properties of nylon 12 by the electron beam irradiation.

Comparative Analysis of Calculation Methods on Willingness to Pay for Introduction of Emergency-call System (교통사고 긴급통보시스템 도입을 위한 지불의사액 산정방안 비교분석)

  • Lee, Yoonjung;Do, Myungsik;Jang, Taek young;Han, Daeseok
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.14 no.6
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    • pp.50-59
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    • 2015
  • This study aimed at suggesting Willingness To Pay (WTP) for introduction of the Traffic Accident emergency Call (TAC) system by using Contingent Valuation Method (CVM) which is a general valuation method. As the method, this study suggested a WTP estimation method of the TAC system with the double-bound dichotomous choice model. In previous studies, the data are processed differently according to the type of questions and analysis models used for the calculation of willingness to pay. Therefore, we re-organized the model by the cases using the truncated data sets, and showed the difference in WTPs. The dataset was developed by more than 500 questionnaire obtained from online and offline survey with the consideration of composition ratio by age group referring housing census in 2010 to mitigate regional bias of samples. At last, this study applied various statistical methods, survival analysis, multiple regression, and Tobit model for better interpretation of the questionnaires.

Development of Wide-Band Compensation Film to Improve Viewing Angle of Vertical Alignment Liquid Crystal Display (수직배향 액정디스플레이의 시야각 향상을 위한 광대역 보상필름 개발)

  • Choi, Yu-Jin;Lim, Young-Jin;Jeong, Kwang-Un;Lee, Seung-Hee
    • Polymer(Korea)
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    • v.35 no.4
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    • pp.337-341
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    • 2011
  • To expand the viewing angle of vertical alignment liquid crystal display (VA-LCD), a wide-band compensation film has been newly developed. VA-LCD needs a retardation film with improved oblique viewing property. The retardation film for VA-LCD has been fabricated by solvent casting and subsequent drawing triacetylcellulose (TAC) containing optical additives. Here, optical birefringence and wavelength dispersion of the retardation film strongly depend on the chemical structures of optical additives. Utilizing quantum mechanical computation, the polarizing anisotropy of retardation film was calculated with respect to additives. It is also realized that the wavelength dispersion of polarizing anisotropy depends on the type and location of substituent to the selected main isomer. When two propionates are substituted to the meta position of the selected main isomer, the wavelength dispersion of polarizing anisotropy shows the most gentle slop, which is well matched with the experimental results. The most gentle slop of wavelength dispersion means that the viewing angle characteristics of LCD are less influenced according to the wavelength of light source of LCD, and it's possible to make better image quality than the present level.