• Journal of Veterinary Science
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• v.21 no.2
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• pp.26.1-26.14
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• 2020

Pancreatic ductal adenocarcinoma is a lethal cancer type that is associated with multiple gene mutations in somatic cells. Genetically engineered mouse is hardly applicable for developing a pancreatic cancer model, and the xenograft model poses a limitation in the reflection of early stage pancreatic cancer. Thus, in vivo somatic cell gene engineering with clustered regularly interspaced short palindromic repeats is drawing increasing attention for generating an animal model of pancreatic cancer. In this study, we selected Kras, Trp53, Ink4a, Smad4, and Brca2 as target genes, and applied Campylobacter jejuni Cas9 (CjCas9) and Streptococcus pyogens Cas9 (SpCas9) for developing pancreatic cancer using adeno associated virus (AAV) transduction. After confirming multifocal and diffuse transduction of AAV2, we generated SpCas9 overexpression mice, which exhibited high double-strand DNA breakage (DSB) in target genes and pancreatic intraepithelial neoplasia (PanIN) lesions with two AAV transductions; however, wild-type (WT) mice with three AAV transductions did not develop PanIN. Furthermore, small-sized Cjcas9 was applied to WT mice with two AAV system, which, in addition, developed high extensive DSB and PanIN lesions. Histological changes and expression of cancer markers such as Ki67, cytokeratin, Mucin5a, alpha smooth muscle actin in duct and islet cells were observed. In addition, the study revealed several findings such as 1) multiple DSB potential of AAV-CjCas9, 2) peri-ductal lymphocyte infiltration, 3) multi-focal cancer marker expression, and 4) requirement of > 12 months for initiation of PanIN in AAV mediated targeting. In this study, we present a useful tool for in vivo cancer modeling that would be applicable for other disease models as well.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.42 no.3
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• pp.427-438
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• 2022

With the recent growing importance over safety management the need for advanced and technical approaches for on-site safety inspection methods has increased. Railway construction is subject to its own particular set of temporal and spatial challenges due to its unique facilities and equipment. This study aimed to investigate the field characteristics of railway infrastructure and improve the conventional field safety management methods by identifying the most appropriate features of AR technology. Group interviews and surveys were conducted with field safety experts to derive the major problems and inspection needs. Subsequently, various features of AR, such as BIM model projection, and remote conferencing, were investigated to determine their applicability to address safety issues. As a result, four problems in the current safety management process, such as 'lack of time due to the conventional inspection method and inspection of areas that are difficult to access', and three major inspection types, such as 'observance of work procedures, status of installation, adequate dimensional spacing', were identified to be improved when adopting AR based techniques. Furthermore, AR technology utilizing plans to solve safety inspection problems and effectively manage major inspection types were proposed, and a follow up survey was conducted with the same field safety experts to derive the priority of technology development.

• Journal of Navigation and Port Research
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• v.46 no.6
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• pp.491-500
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• 2022

It has been noted that an accurate estimation of wind loads on offshore structures such as an FLNG (Liquefied Natural Gas Floating P roduction Storage Offloading Units, LNG FPSOs) with a large topside plays an important role in the safety design of hull and mooring system. Therefore, the present study aims to develop a computational model for estimating the wind load acting on an FLNG. In particular, it is the sequel to the previous research by the author. The numerical computation model in the present study was modified based on the previous research. Numerical analysis for estimating wind loads was performed in two conditions for an interval of wind direction (α), 15° over the range of 0° to 360°. One condition is uniform wind speed and the other is the NPD model reflecting the wind speed profile. At first, the effect of sand-grain roughness on the speed profile of the NPD model was studied. Based on the developed NPD model, mesh convergence tests were carried out for 3 wind headings, i.e. head, quartering, and beam. Finally, wind loads on 6-degrees of freedom were numerically estimated and compared by two boundary conditions, uniform speed, and the NPD model. In the present study, a commercial RANS-based viscous solver, STAR-CCM+ (ver. 17.02) was adopted. In summary, wind loads in surge and yaw from the wind speed profile boundary condition were increased by 20.35% and 34.27% at most. Particularly, the interval mean of sway (45° < α <135°, 225° < α < 315°) and roll (60° < α < 135°, 225° < α < 270°) increased by 15.60% and 10.89% against the uniform wind speed (10m/s) boundary condition.

• The Journal of the Korea institute of electronic communication sciences
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• v.17 no.6
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• pp.1249-1258
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• 2022

Marine debris is defined as a substance that is intentionally or inadvertently left on the shore or is introduced or discharged into the ocean, which has or is likely to have a harmful effect on the marine environments. In this study, the detection of marine debris and the analysis of the amount of change on marine debris were performed using the object detection method for an efficient method of identifying the quantity of marine debris and analyzing the amount of change. The study area is Yuho Mongdol Beach in the northeastern part of Geoje Island, and the amount of change was analyzed through images collected at 15-minute intervals for 32 days from September 12 to October 14, 2022. Marine debris detection using YOLOv5x, a one-stage object detection model, derived the performance of plastic bottles mAP 0.869 and styrofoam buoys mAP 0.862. As a result, marine debris showed a large decrease at 8-day intervals, and it was found that the quantity of Styrofoam buoys was about three times larger and the range of change was also larger.

• Journal of the Korea Institute of Building Construction
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• v.22 no.6
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• pp.585-596
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• 2022

The purpose of this study is to propose an assessment method to analyze clay collectively referred to as fine particles of clay components contained in fine aggregates, and to propose quality standards for clay use through correlation with the performance of concrete to verify the properties of clay measured according to the method. As a result, it is analyzed that it will be suitably utilized as a method to assess the fine particles of the clay component of fine aggregates through the component analysis of XRF. Regarding the related quality standards, considering the error rate of about 10% of KCS 14 20 10, the related quality standards were analyzed to be safe when Al₂O₃+Fe₂O₃+MgO is 23.5% or less and SiO₂+K₂OSiO₂+K2₂O is 66.5% or more. To build on this study, it is expected that a comprehensive review will be conducted through additional follow-up studies such as on clay of coarse aggregates and durability analysis to establish a system for quality control of the soil fraction of aggregates.

• Journal of the Korean Geotechnical Society
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• v.38 no.12
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• pp.7-17
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• 2022

The ratios of water and controlled low-strength materials (CLSM) were selected as 1:0.4, 1:0.6, 1:0.8, 1:1.0, and 1:1.2 to minimize the construction and long-term decrease in uniaxial compressive strength due to dry shrinkage through the combination of water, CLSM, and expansion agent. Approximately 2% and 5% of the expansion agent were added for each blending condition. As a result, it was found that the compressive strength decreased and the expandability increased as the specific gravity of the water increased. In addition, it was confirmed that the compressive strength increased rapidly up to 15 days of age compared to the CLSM used in the field. However, the compressive strength decreased compared to the 15 days of the age as of the 28 days of the age. It showed engineering characteristics similar to CLSM generally used in the field. Therefore, the water and the CLSM were mixed at a ratio of 1:0.8, and the field test was performed by adding 5% of an expansion agent. As a result, 28 days after age, the cavity waveform was observed using the handy GPR exploration system, and it was found that cavity waveform was relaxed or disappeared.

• Journal of the Korean Geotechnical Society
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• v.38 no.7
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• pp.25-37
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• 2022

In this paper, a small-scale model testing system was developed using a series of small-scale model tests to analyze the mechanism of compaction pile formation and evaluate the quality of controlled grading aggregates proposed as an alternative material to the sand compaction pile (SCP) method and granular compaction pile (GCP). These are the most typical ground improvement methods in field practice, particularly for soft grounds. However, the SCP has faced difficulties due to the supply shortage of natural sand and the corresponding price surge of sand. The GCP is limited in marine soft grounds because of the failure occurring at the pile tip caused by excessive expansion of the deeper bulbs, leading to uneven bulb formation. The uniformity of compacted pile bulbs is critical to ensuring the bearing capacity and quality of the compaction pile. This study aims to evaluate the performance of the new material and controlled grading aggregates using small-scale model tests simulating field compaction process to investigate its potential application in comparison with SCP. The compaction piles are examined in four cases according to different materials used for compaction pile and clay strength. The compaction pile materials, which are made of sand and controlled grading aggregates, used in this study were compared to reveal the mechanism of the bulb creation. The experimental data confirm that the bulb formation quality of the traditional sand and the new material, controlled grading aggregates are comparable. The compaction pile made of controlled grading aggregates presents higher bearing capacity than that of marine sand.

• Journal of Korea Water Resources Association
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• v.55 no.6
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• pp.405-419
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• 2022

Flood frequency analysis commonly used to design the hydraulic structures to minimize flood damage includes uncertainty. Therefore, the most appropriate design flood within a uncertainty should be selected in the final stage of a hydraulic structure, but related studies were rarely carried out. The total expected cost function introduced into the flood frequency analysis is a new approach for determining the optimal design flood. This procedure has been used as UNCODE (UNcertainty COmpliant DEsign), but the application has not yet been introduced in South Korea. This study introduced the mathematical procedure of UNCODE and calculated the optimal design flood using the annual maximum inflow of hydroelectric dams located in the Bukhan River system and results were compared with that of the existing flood frequency. The parameter uncertainty was considered in the total expected cost function using the Gumbel and the GEV distribution, and the Metropolis-Hastings algorithm was used to sample the parameters. In this study, cost function and damage function were assumed to be a first-order linear function. It was found that the medians of the optimal design flood for 4 Hydroelectric dams, 2 probability distributions, and 2 return periods were calculated to be somewhat larger than the design flood by the existing flood frequency analysis. In the future, it is needed to develop the practical approximated procedure to UNCODE.

• Quality Improvement in Health Care
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• v.28 no.1
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• pp.45-54
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• 2022

Purpose: The study aims to identify the theme list and priority criteria of patient safety theme reports in South Korea. Methods: The survey was conducted twice, and the importance of each criterion and theme was measured on a nine-point scale using the Delphi technique by a panel of 19 patient safety experts. The criteria included severity, universality, preventability, and organizational-social impact. Descriptive statistics such as frequency, percentage, mean, standard deviation, median, and interval quartile range were used to analyze the data. Results: The parameters were assigned a weighted average of 35% for severity, 20% for universality, 30% for preventability, and 15% for organizational-social impact, respectively. The final top three rankings were surgery safety, blood transfusion safety, and medication safety. In addition to expert opinion, for the theme that is selected based on the priority ranking, one to five sub-topics can be derived from the theme based on the priority ranking, societal demands, or the yearly priority list of patient safety incidents. Conclusion: It is recommended that the official patient safety center distribute the report in the form of a summary that can be utilized nationwide at medical institutions, government institutions, and other places. Updates, as well as accumulated theme reports, will serve as the baseline data for the proposal of the system and for the policy designed to implement and improve institutions' safety practices as a standard of domestic patient safety practice guidelines.

• Journal of the Korea Institute of Building Construction
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• v.22 no.2
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• pp.137-148
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• 2022

Recently, in the concrete industry, the development and commercialization of low-carbon products of ready-mixed concrete have emerged as part of the efforts to realize carbon neutrality. This study aims to investigate the current status of environmental product declaration(EPD) of ready-mixed concrete and to analyze the characteristics of carbon emissions by compressive strength, life cycle stage, and region. To this end, the related certification system requiring the calculation of carbon emissions in the concrete industry was analyzed. The target of analyzing the current status of carbon emissions was set as a product of ready-mixed concrete that acquired EPD certification based on the life cycle assessment method. In addition, the trend of carbon emissions according to each characteristic was reviewed by analyzing carbon emissions by the life cycle of ready-mixed concrete products, analyzing carbon emissions by standard, and analyzing carbon emissions by region. As a result, the carbon emissions in the pre-production stage were 99% compared to total carbon emissions., and as it increased from 18MPa to 40MPa, carbon emissions also increased. Even with the same specifications, the carbon emissions in the capital region were higher than in the southern region.