• 한국산림과학회지
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• 제82권1호
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• pp.7-11
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• 1993

상해(傷害)에 기인하여 양버즘나무(Platanus occidentalis L.) 수간에 형성된 팽창조직(膨脹組織)의 해부학적(解剖學的) 특성(特性)을 조직학적(組織學的) 및 수량적(數量的) 측면(側面)네서 정상조직(正常組織)과 비교(比較) 고찰(考察)하였다. 팽창조직은 정상조직에 비하여 세포배열이 불규칙적이고 도관요소내 타일로시스가 현저하게 발달되어 있으며, 후벽화(厚壁化)된 목섬유가 흔히 관찰된다는 점, 복합관공이 덜 나타난다는 점에서 차이를 나타내었고, 관공의 직경이 작고 도관요소와 목섬유의 길이가 짧으며 도관빈도(vessel frequency)는 작은 반면, 도관요소내 계단상 천공관의 bar 수(數)가 많으며, 방사조직 빈도(ray frequency)가 크고 방사조직의 폭이 넓으나 높이가 작은 특성(特性)을 나타내었다.

• 생물환경조절학회지
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• 제23권3호
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• pp.167-173
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• 2014

고온기 시설내에서 토마토를 재배할 때 최적의 측지관리방법을 구명하고자 본 실험을 수행하였다. 유니콘(몬산토 코리아, 한국)을 접수로, B-블로킹(다끼이종묘, 일본)을 대목으로 접목한 방울토마토 접목묘를 실험에 사용하였다. 배지는 코이어 자루배지를 사용하였고, 급액은 타이머 제어법으로 제어하였다. 측지를 전부 제거한 처리(ACUT), 화방 아래 측지의 잎을 2매 남기는 처리(PCUT) 및 모든 측지의 잎을 2매 남기는 처리(LEFT) 등 모두 3가지 방법으로 처리하였다. 연구결과, 토마토의 영양과잉으로 인한 이상경 발생시에는 측지를 유지하여 영양생장으로 많은 에너지가 사용되도록 하면 해결되는 것으로 나타났으며, 적절한 측지관리로 작물의 생장상도 재배자의 요구에 맞게 조절할 수 있을 것으로 사료되었다. 본엽과 측지의 잎들에 대한 광합성 속도는 차이가 없었으며, 처리에 따른 엽면적의 차이만 있었다. 따라서 처리간 엽면적의 차이에 의해 광합성 산물 총량의 차이가 발생하고, 이는 수확량에 영향을 주는 것을 확인하였다. 또한 고온기 토마토 재배에서 5단 이하의 단기밀식재배의 경우에는 측지를 모두 제거하는 것이 수확량과 수확속도에 효과적이었으나, 5단 이상의 장기재배에서는 모든 측지의 잎 2매를 남겨서 관리하는 것이 작물의 생육과 수확량에 효과적이었다.

• The Plant Pathology Journal
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• 제24권2호
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• pp.112-117
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• 2008

Pre-infection behavior of Fusarium circinatum on stems of pine species was investigated with scanning electron microscopy. Two-year-old stems of Pinus densiflora and p. rigida were inoculated with the fungal conidial suspension and subjected to $25^{\circ}C$ for up to 16 hr. Most microconidia germinated 12 hr after inoculation on pine stems. Conidia produced germ tubes from either one or both ends of microconidia. Germ tubes grew over the stem surface and appeared to enter host tissues through natural openings on pine stems. Surface cracks in the cork were entrance sites of germ tubes of F. circinatum. In addition, host cell wall cracks were often found at the tip of germ tubes. The cuticle appeared to be eroded either at the tip of germ tubes or around germlings. Germ tubes also produced appressoria-like structures, exhibiting swollen tips of germ tubes on the stem surface. There seems to be no significant differences in the pre-infection behavior of F. circinatum on stems between the two pine species.

• 식물병연구
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• 제8권4호
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• pp.224-227
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• 2002

실내 장식용 드라세나 산데리아의 줄기에 발생한 괴사 병징으로부터 탄저병원균을 확인하였다. 분생자층 위에 분생포자, 강모 및 자낭각이 관찰되었다 분생포자는 무색, 단포, 정부는 약간 둥글고 기부는 굵고 원통형 또는 약간 타원형으로 크기는 12.5-17.5 $\mu\textrm{m}$였다. 강모는 격막이 1~4 개 존재하며, 기부세포가 특징적으로 팽대되고 크기는 200 $\mu\textrm{m}$였다 부착기는 암갈색, 난형으로 크기는 5-12.5 $\mu\textrm{m}$이며, 괴사된 줄기상의 자낭각은 구형, 암갈색 또는 검은색으로 크기는 85-300 $\mu\textrm{m}$, 자낭은 곤봉형 또는 원통형이며 크기는 50-62.5$\times$8-10 $\mu\textrm{m}$로서 8개의 자낭포자를 형성하였다. 자낭포자는 타원형 또는 굽은 방추형이었다. 이 균의 불완전세대의 특징을 C. musae와 C. lindemuthianum과 비교하여 Glomerella cingulata(C. gloeosporioides)로 동정하였다.

• The Plant Pathology Journal
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• 제32권2호
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• pp.85-94
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• 2016

Studies were conducted to determine the role of 3-methylthioproprionic acid (MTPA) in the pathogenicity of potato stem canker, Rhizoctonia solani, and the concentrations required to inhibit growth of R. solani under laboratory and plant house-based conditions. The experiments were laid out in a completely randomized design with five treatments and five replications. The treatments were 0, 1, 2, 4, and 8 mM concentrations of MTPA. The purified toxin exhibited maximal activity at pH 2.5 and $30^{\circ}C$. MTPA at 1, 2, 4, and 8 mM levels reduced plant height, chlorophyll content, haulm fresh weight, number of stolons, canopy development, and tuber weight of potato plants, as compared to the control. MTPA significantly affected mycelial growth with 8 mM causing the highest infection. The potato seedlings treated with MTPA concentrations of 1.0-8.0 mM induced necrosis of up to 80% of root system area. Cankers were resulted from the injection of potato seedling stems with 8.0 mM MTPA. The results showed the disappearance of cell membrane, rough mitochondrial and cell walls, change of the shape of chloroplasts, and swollen endoplasmic reticulum. Seventy-six (76) hours after toxin treatment, cell contents were completely broken, cytoplasm dissolved, and more chromatin were seen in the nucleus. The results suggested that high levels of the toxin concentration caused cell membrane and cytoplasm fracture. The integrity of cellular structure was destroyed by the phytotoxin. The concentrations of the phytotoxin were significantly correlated with pathogenicity and caused damage to the cell membrane of potato stem base tissue.

• 한국산림과학회지
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• 제96권5호
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• pp.515-519
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• 2007

An imperfect fungus Fusiococcum species was isolated from Quercus dentata. A naturally infected Daimyo oak tree was collected and showed elongate wounds on the stem. The fungal cultures were initially white and cottony, and later turned dark gray. Numerous solitary pycnidia were developed on the medium surface, and typically spherical. Yellowish conidial masses were exuded from pycnidia on the culture plates. Conidial masses were swollen and measured as approximately 100 to $300{\mu}m$ in length. It appeared that conidia were usually held together in globose to oval drops. Conidia were hyaline, single-celled (nonseptate), ellipsoid to fusoid, and measured as approximately $8.0{\times}2.7{\mu}m$. Based on these cultural and morphological characteristics, the fungal isolate was identified as a species of Fusicoccum Corda. To preserve and examine fungal spores exuded from pycnidia on the medium surface, a vapor fixation procedure for scanning electron microscopy was employed in this study. The specimens were exposed to the vapor of 2% (v/v) glutaraldehyde and 2% (w/v) osmium tetroxide each for 2 h. With the vapor fixation we obtained excellent retention of conidial masses in this study. The simple and versatile procedure for demonstrating fungal spores and their exudation from fruiting bodies would facilitate characterization of diverse pathological and environmental isolates as they are in native environments.

• The Plant Pathology Journal
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• 제19권3호
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• pp.181-183
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• 2003

Corky root symptoms caused by Pyrenochaeta lycopersici were observed on the roots and stem base of tomato plants in Korea. Symptoms on infected plants typically appeared as stunting and generally lacking vigor, and infected plants die back from the foliage tips after fruits have set. Brown lesions appearing with bands around the roots were characteristic symptoms of the disease. The lesions become swollen and cracked along the length of the root with corky appearance. Based on cultural and morphological characteristics, the fungus from the diseased plants was identified as Pyrenochaeta lycopersici. Pycnidia were solitary, globose to subglobose, brown to black, darker around the neck region, and measured 173-215 $\mu\textrm{m}$ in diameter with septate setae up to 102-132$\times$6.5 $\mu\textrm{m}$. Conidia were hyaline, unicellular, and 4.2-4.7$\times$l.5-2.0 $\mu\textrm{m}$ long. Optimum temperature for mycelial growth of the p. lycopersici isolates ranged from $20^{\circ}C$ to $25^{\circ}C$. Fifteen isolates off lycopersici were tested for pathogenicity to susceptible and tolerant cultivars of tomato plants by artificial inoculation. Three isolates of P. lycopersici induced typical corky root discoloration on susceptible tomato cultivars but not on tolerant tomato. This is the Erst report in Korea of tomato corky root disease caused by P. lycopersici.