• Journal of Korean Society of Forest Science
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• v.82 no.1
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• pp.7-11
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• 1993

The swollen tissue of wound origin formed after nailing ㄷ-shaped fastener to a stem of Platanus occidentalis L. was compared with normal tissue in the qualitative and quantitative anatomical aspects. The swollen tissue appeared to differ from normal tissue in irregular cell orientation, occurrence of tyloses in vessel element, less common appearance of pore multiples, narrower and shorter vessel element, lower vessel frequency, shorter and highly thick-walled wood fiber, greater number of bars per scalariform perforation plate in vessel element, larger ray frequency, larger ray width and lower ray height.

• Journal of Bio-Environment Control
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• v.23 no.3
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• pp.167-173
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• 2014

This research was conducted to establish appropriate methods to prune tomato side stems during summer. Cherry tomatoes "Unicorn" (Monsanto Korea, Korea) were grown in the coir based growing medium, and irrigation was controlled time based system. There were three pruning treatments: 1) removing all side stems (ACUT), 2) remaining two leaves on the side stems right below any cluster (PCUT), and 3) remaining two leaves on all side stems (LEFT). Experimental results showed that the occurrence of swollen stems, a symptom of nutrient excess, was influenced by side stem pruning due to blocking of consumption of photosynthetic products. The photosynthetic rate was not different between leaves on main stem and those on side shoots. Therefore the differences in the total amounts of photosynthetic products seemed to come out from the differences in leaf areas on each treatments, influencing on fruit yield difference. The yields and harvesting rates were better in ACUT treatment when tomato plants were harvested until $5^{th}$ cluster, however tomato yield was higher in LEFT treatment when more then $5^{th}$ clusters were harvested.

• The Plant Pathology Journal
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• v.24 no.2
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• pp.112-117
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• 2008

Pre-infection behavior of Fusarium circinatum on stems of pine species was investigated with scanning electron microscopy. Two-year-old stems of Pinus densiflora and p. rigida were inoculated with the fungal conidial suspension and subjected to $25^{\circ}C$ for up to 16 hr. Most microconidia germinated 12 hr after inoculation on pine stems. Conidia produced germ tubes from either one or both ends of microconidia. Germ tubes grew over the stem surface and appeared to enter host tissues through natural openings on pine stems. Surface cracks in the cork were entrance sites of germ tubes of F. circinatum. In addition, host cell wall cracks were often found at the tip of germ tubes. The cuticle appeared to be eroded either at the tip of germ tubes or around germlings. Germ tubes also produced appressoria-like structures, exhibiting swollen tips of germ tubes on the stem surface. There seems to be no significant differences in the pre-infection behavior of F. circinatum on stems between the two pine species.

• Research in Plant Disease
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• v.8 no.4
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• pp.224-227
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• 2002

From the necrotic stem of aquaculture Dracaena for living room decoration, we observed conidia on acervuli with occasional setae and perithecia in vivo. Morphological characters were; acervuli, dark brown and 300-500 ${\mu}{\textrm}{m}$ in diameter; conidia, hyaline, aseptate, cylindrical to slightly ellipsoid with rounded apex and narrow truncate base on phialidic conidiophores, 12.5-17.5 ${\mu}{\textrm}{m}$; setae, variable in length 1-4 septate, slightly swollen at the base and tapered to the apex, 200 ${\mu}{\textrm}{m}$; appressoria, dark brown ovate to obovate irregular 5-12.5 ${\mu}{\textrm}{m}$, perithecia on decayed stem, globose, dark brown and black 85-300 ${\mu}{\textrm}{m}$; asci 8 spored, clavate to cylindrical; 50-62.5$\times$8-10 ${\mu}{\textrm}{m}$; ascospores, oval to fusiform, sometimes slightly curved, aseptate, hyaline. Above characteristics were also confirmed in vitro. Colletotrichum state of this fungus was also compared with C. lindemuthianum and C. musae in terms of morphology of conidia and setae and growth on PDA etc. This fungus was identified as Glomerella cingulata(Stonem.) Spaulding & Schrenk(Colletotrichum gloeosporioides(Penz. Sacc.).

• The Plant Pathology Journal
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• v.32 no.2
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• pp.85-94
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• 2016

Studies were conducted to determine the role of 3-methylthioproprionic acid (MTPA) in the pathogenicity of potato stem canker, Rhizoctonia solani, and the concentrations required to inhibit growth of R. solani under laboratory and plant house-based conditions. The experiments were laid out in a completely randomized design with five treatments and five replications. The treatments were 0, 1, 2, 4, and 8 mM concentrations of MTPA. The purified toxin exhibited maximal activity at pH 2.5 and $30^{\circ}C$. MTPA at 1, 2, 4, and 8 mM levels reduced plant height, chlorophyll content, haulm fresh weight, number of stolons, canopy development, and tuber weight of potato plants, as compared to the control. MTPA significantly affected mycelial growth with 8 mM causing the highest infection. The potato seedlings treated with MTPA concentrations of 1.0-8.0 mM induced necrosis of up to 80% of root system area. Cankers were resulted from the injection of potato seedling stems with 8.0 mM MTPA. The results showed the disappearance of cell membrane, rough mitochondrial and cell walls, change of the shape of chloroplasts, and swollen endoplasmic reticulum. Seventy-six (76) hours after toxin treatment, cell contents were completely broken, cytoplasm dissolved, and more chromatin were seen in the nucleus. The results suggested that high levels of the toxin concentration caused cell membrane and cytoplasm fracture. The integrity of cellular structure was destroyed by the phytotoxin. The concentrations of the phytotoxin were significantly correlated with pathogenicity and caused damage to the cell membrane of potato stem base tissue.

• Journal of Korean Society of Forest Science
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• v.96 no.5
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• pp.515-519
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• 2007

An imperfect fungus Fusiococcum species was isolated from Quercus dentata. A naturally infected Daimyo oak tree was collected and showed elongate wounds on the stem. The fungal cultures were initially white and cottony, and later turned dark gray. Numerous solitary pycnidia were developed on the medium surface, and typically spherical. Yellowish conidial masses were exuded from pycnidia on the culture plates. Conidial masses were swollen and measured as approximately 100 to $300{\mu}m$ in length. It appeared that conidia were usually held together in globose to oval drops. Conidia were hyaline, single-celled (nonseptate), ellipsoid to fusoid, and measured as approximately $8.0{\times}2.7{\mu}m$. Based on these cultural and morphological characteristics, the fungal isolate was identified as a species of Fusicoccum Corda. To preserve and examine fungal spores exuded from pycnidia on the medium surface, a vapor fixation procedure for scanning electron microscopy was employed in this study. The specimens were exposed to the vapor of 2% (v/v) glutaraldehyde and 2% (w/v) osmium tetroxide each for 2 h. With the vapor fixation we obtained excellent retention of conidial masses in this study. The simple and versatile procedure for demonstrating fungal spores and their exudation from fruiting bodies would facilitate characterization of diverse pathological and environmental isolates as they are in native environments.

• The Plant Pathology Journal
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• v.19 no.3
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• pp.181-183
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• 2003

Corky root symptoms caused by Pyrenochaeta lycopersici were observed on the roots and stem base of tomato plants in Korea. Symptoms on infected plants typically appeared as stunting and generally lacking vigor, and infected plants die back from the foliage tips after fruits have set. Brown lesions appearing with bands around the roots were characteristic symptoms of the disease. The lesions become swollen and cracked along the length of the root with corky appearance. Based on cultural and morphological characteristics, the fungus from the diseased plants was identified as Pyrenochaeta lycopersici. Pycnidia were solitary, globose to subglobose, brown to black, darker around the neck region, and measured 173-215 $\mu\textrm{m}$ in diameter with septate setae up to 102-132$\times$6.5 $\mu\textrm{m}$. Conidia were hyaline, unicellular, and 4.2-4.7$\times$l.5-2.0 $\mu\textrm{m}$ long. Optimum temperature for mycelial growth of the p. lycopersici isolates ranged from $20^{\circ}C$ to $25^{\circ}C$. Fifteen isolates off lycopersici were tested for pathogenicity to susceptible and tolerant cultivars of tomato plants by artificial inoculation. Three isolates of P. lycopersici induced typical corky root discoloration on susceptible tomato cultivars but not on tolerant tomato. This is the Erst report in Korea of tomato corky root disease caused by P. lycopersici.