• Journal of the Korean housing association
• /
• v.26 no.2
• /
• pp.1-12
• /
• 2015

The era of globalization has ushered in dramatic changes in the past decade covering a wide range of distinct political, economic and cultural trends whereby people adapt their houses to suit their needs and desires. This paper discusses the process of change analytically, emphasizing the importance of understanding the transformation of Lao traditional houses and its impact factors to the transformation and adaptation to suit with globalization trend, and conservation of the characteristics of traditional houses. This study takes place in Luangprabang, an ancient city of Lao PDR, which was designated as a world heritage city in 1995 as it is rich with diverse, tangible and intangible values. Five cases of traditional house in Luangprabang are examined to understand their transformation through time, by analyzing the basic spatial formation, components and elements, especially to find out the impact factors to such transformation. The study revealed that the transformation of the traditional house is subject to not just one single factor, but to a number of factors, where globalization, regulation, economy, and social aspect factors play a prime role and are the root for all these changes. The case study also indicates that these changes are connected to the benefits of income generation as a survival strategy for the low and middle-income people in Luangprabang as well as the housing demands. In the transition period a new residential type appeared accordingly, in which living spaces were categorized according to their general functions. There was a harmony between people's needs and the physical characteristics of the house.

• Development and Reproduction
• /
• v.18 no.2
• /
• pp.107-115
• /
• 2014

Cardiovascular system is the primary organ to develop and reach a functional state, which underscores the essential role of the vasculature in the developing embryo. The vasculature is a highly specialized organ that functions in a number of key physiological works including the carrying of oxygen and nutrients to tissues. It is closely involved in the formation of heart, and hence it is essential for survival during the hatching period. The expression of genes involved during vascular development in the olive flounder (Paralichthys olivaceus) in the days after hatching is not fully understood. Therefore, we examined the expression patterns of genes activated during the development of flounder. Microscopic observations showed that formation of blood vessels is related to the expression of the vimentin gene. Also, the temporal expression patterns of this vimentin-like gene in the developmental stages and in the normal tissues of olive flounder. The purpose of this study was to examine the expression patterns of vimentin in normal tissues of the olive flounder and during the development of the vascular system in newly hatched olive flounders and HIF-1 plays a vital role in the formation of blood vessels during development. Vimentin expression was strong at the beginning of the development of blood vessels, and was present throughout all developmental stages. Our findings have important implications with respect to the roles of vimentin and HIF-1 in the development and evolution of the first blood vessels in olive flounder. Further studies are required to elucidate the vimentin-mediated hypoxic response signal transduction and to decipher the functional role of vimentin in developmental stages.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.16
• /
• pp.7277-7284
• /
• 2015

Background: The Wnt/${\beta}$-catenin signaling pathway is an important regulator of cellular functions such as proliferation, survival and cell adhesion. Wnt/${\beta}$-catenin signaling is associated with tumor initiation and progression; ${\beta}$-catenin mutations explain only 30% of aberrant signaling found in breast cancer, indicating that other components and/or regulation of the Wnt/${\beta}$-catenin pathway may be involved. Objective: We evaluated AXIN2 rs2240308 and rs151279728 polymorphisms, and expression profiles of ${\beta}$-catenin destruction complex genes in breast cancer patients. Materials and Methods: We collected peripheral blood samples from 102 breast cancer and 102 healthy subjects. The identification of the genetic variation was performed using PCR-RFLPs and DNA sequencing. RT-qPCR was used to determine expression profiles. Results: We found significant association of AXIN2 rs151279728 and rs2240308 polymorphisms with breast cancer risk. Significant increase was observed in AXIN2 level expression in breast cancer patients. Further analyses showed APC, ${\beta}$-catenin, CK1${\alpha}$, GSK3${\beta}$ and PP2A gene expression to be associated to clinic-pathological characteristics. Conclusions: The present study demonstrated, for the first time, that AXIN2 genetic defects and disturbance of ${\beta}$-catenin destruction complex expression may be found in breast cancer patients, providing additional support for roles of Wnt/${\beta}$-catenin pathway dysfunction in breast cancer tumorigenesis. However, the functional consequences of the genetic alterations remain to be determined.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.11
• /
• pp.4405-4409
• /
• 2014

Reactive oxygen species (ROS), highly reactive molecules, are produced by living organisms as a result of normal cellular metabolism and environmental factors, and can damage nucleic acids and proteins, thereby altering their functions. The human body has several mechanisms to counteract oxidative stress by producing antioxidants. A shift in the balance between oxidants and antioxidants in favor of oxidants is termed as "oxidative stress". Paradoxically, there is a large body of research demonstrating the general effect of oxidative stress on signaling pathways, less is known about the initial and direct regulation of signaling molecules by ROS, or what we term the "oxidative interface." This review focuses on the molecular mechanisms through which ROS directly interact with critical signaling molecules to initiate signaling in a broad variety of cellular processes, such as proliferation and survival (MAP kinases and PI3 kinase), ROS homeostasis, and antioxidant gene regulation (Ref-1 and Nrf-2). This review also deals with classification as well as mechanisms of formation of free radicals, examining their beneficial and deleterious effects on cellular activities and focusing on the potential role of antioxidants in preventing and repairing damage caused by oxidative stress. A discussion of the role of phytochemical antioxidants in oxidative stress, disease and the epigenome is included.

• The Plant Pathology Journal
• /
• v.34 no.1
• /
• pp.44-58
• /
• 2018

Pseudomonas chlororaphis 30-84 is a biological control agent selected for its ability to suppress diseases caused by fungal pathogens. P. chlororaphis 30-84 produces three phenazines: phenazine-1-carboxylic acid (PCA), 2-hydroxy-phenazine-1-carboxylic acid (2OHPCA) and a small amount of 2-hydroxy-phenazine (2OHPHZ), and these are required for fungal pathogen inhibition and wheat rhizosphere competence. The two, 2-hydroxy derivatives are produced from PCA via the activity of a phenazine-modifying enzyme encoded by phzO. In addition to the seven biosynthetic genes responsible for the production of PCA, many other Pseudomonas strains possess one or more modifying genes, which encode enzymes that act independently or together to convert PCA into other phenazine derivatives. In order to understand the fitness effects of producing different phenazines, we constructed isogenic derivatives of P. chlororaphis 30-84 that differed only in the type of phenazines produced. Altering the type of phenazines produced by P. chlororaphis 30-84 enhanced the spectrum of fungal pathogens inhibited and altered the degree of take-all disease suppression. These strains also differed in their ability to promote extracellular DNA release, which may contribute to the observed differences in the amount of biofilm produced. All derivatives were equally important for survival over repeated plant/harvest cycles, indicating that the type of phenazines produced is less important for persistence in the wheat rhizosphere than whether or not cells produce phenazines. These findings provide a better understanding of the effects of different phenazines on functions important for biological control activity with implications for applications that rely on introduced or native phenazine producing populations.

• Molecules and Cells
• /
• v.39 no.11
• /
• pp.821-826
• /
• 2016

The ${\beta}$-catenin functions as an adhesion molecule and a component of the Wnt signaling pathway. In the absence of the Wnt ligand, ${\beta}$-catenin is constantly phosphorylated, which designates it for degradation by the APC complex. This process is one of the key regulatory mechanisms of ${\beta}$-catenin. The level of ${\beta}$-catenin is also controlled by the E3 ubiquitin protein ligase SIAH-1 via a phosphorylation-independent degradation pathway. Similar to ${\beta}$-catenin, STAT3 is responsible for various cellular processes, such as survival, proliferation, and differentiation. However, little is known about how these molecules work together to regulate diverse cellular processes. In this study, we investigated the regulatory relationship between STAT3 and ${\beta}$-catenin in HEK293T cells. To our knowledge, this is the first study to report that ${\beta}$-catenin-TCF-4 transcriptional activity was suppressed by phosphorylated STAT3; furthermore, STAT3 inactivation abolished this effect and elevated activated ${\beta}$-catenin levels. STAT3 also showed a strong interaction with SIAH-1, a regulator of active ${\beta}$-catenin via degradation, which stabilized SIAH-1 and increased its interaction with ${\beta}$-catenin. These results suggest that activated STAT3 regulates active ${\beta}$-catenin protein levels via stabilization of SIAH-1 and the subsequent ubiquitin-dependent proteasomal degradation of ${\beta}$-catenin in HEK293T cells.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.25 no.6
• /
• pp.1399-1410
• /
• 2015

Gartner is requiring companies to considerably change their survival paradigms insisting that companies need to understand and provide again the upcoming era of data competition. With the revealing of successful business cases through statistic algorithm-based predictive analytics, also, the conversion into preemptive countermeasure through predictive analysis from follow-up action through data analysis in the past is becoming a necessity of leading enterprises. This trend is influencing security analysis and log analysis and in reality, the cases regarding the application of the big data analysis framework to large-scale log analysis and intelligent and long-term security analysis are being reported file by file. But all the functions and techniques required for a big data log analysis system cannot be accommodated in a Hadoop-based big data platform, so independent platform-based big data log analysis products are still being provided to the market. This paper aims to suggest a framework, which is equipped with a real-time and non-real-time predictive analysis engine for these independent big data log analysis systems and can cope with cyber attack preemptively.

• BMB Reports
• /
• v.42 no.11
• /
• pp.752-757
• /
• 2009

Copper is essential but toxic in excess for aerobic organisms. Yeast transcription factor ACE1 functions as a sensor for copper and an inducer for the transcription of CUP1. In addition, ACE1 can activate the transcription of superoxide dismutase gene (sod1) in response to copper. In this study, we introduced the yeast ACE1 into Arabidopsis and analyzed its function in plant. Under high copper stress, the transgenic plants over-expressing ACE1 showed higher survival rate than the wild-type. We also found that over-expression of ACE1 in Arabidopsis increased the activities of SOD and POD, which were beneficial to the cell in copper buffering. Excess copper would suppress the expression of chlorophyll biosynthetic genes in Arabidopsis, RT-PCR analysis revealed that over-expression of ACE1 decrease the suppression. Together, our results indicate that ACE1 may play an important role in response to copper stress in Arabidopsis.

• 한국경영정보학회:학술대회논문집
• /
• 2007.06a
• /
• pp.545-550
• /
• 2007

The aim of this paper is to find out whether there is a difference in adopting and/or diffusing the information and communication technology (ICT) between countries. If there is, what are the primary factors that keep some countries from adopting and diffusing ICT while others do? To analyze the above problem, we adopted the Unified Theory of Acceptance and Use of Technology (UTAUT) suggested by Venkatesh et al. (2003), which consists of effort expectancy, performance expectancy and social influence. We also use the innovation diffusion functions, which are known to have the S-shape and are made up of the introduction, growth, maturity and decline phases. We do not, however, consider the decline phase, because the ICT that we are considering is not believed to be in that phase. Therefore, we researched how the three factors affect adoption in the three phases. We selected the cellular phone as the ICT, because it is considered to be the most popularly used ICT and its technology has been developing rapidly. We surveyed the cellular phone adopters in Korea, and the U.S. for 15 years from 1989 to 2003. Korea, and the U.S. represent newly developed and developed countries, respectively. For the data analysis, a survival analysis was used, as it could explain the characteristics of the potential adopters or non-adopters. We found that the ICT diffusion patterns, as well as the ICT diffusion factors, of the two countries were different. Therefore, we believe that the results of our research can be used in building strategies on reducing the digital divide gaps between countries.

• Korean Journal of Plant Tissue Culture
• /
• v.27 no.4
• /
• pp.339-343
• /
• 2000

When plants are exposed to subfreezing temperatures ice crystals are forming within extracelluar space in leaves. The growth of ice crystal is closely related to the degree of freezing injury. It was shown that an antifreeze protein binds to an ice nucleator through hydrogen bonds to prevent growth of ice crystal and also reduces freezing damage. The antifreeze proteins in plants are similar to PR proteins but only the PR proteins induced upon cold acclimation were shown to have dual functions in antifreezing as well as antifungal activities. Three of the genes encoded for CLP, GLP, and TLP were isolated from barley and Kentucky bluegrass based on amino acid sequence revealed after purification and low temperature-inducibility as shown in analysis of the protein. The deduced amino acid of the genes cloned showed a signal for secretion into extracellular space where the antifreezing activity sup-posed to work. The western analysis using the antisera raised against the antifreeze proteins showed a positive correlation between the amount of the protein and the level of freeze tolerance among different cultivars of barely. Besides it was revealed that TLP is responsible for a freeze tolerance induced by a treatment of trinexapac ethyl in Kentucky bluegrass. Analysis of an overwintering wild rice, Oryza rufipogon also showed that an acquisition of freeze tolerance relied on accumulation of the protein similar to CLP. The more direct evidence for the role of CLP in freeze tolerance was made with the analysis of the transgenic tobacco showing extracellular accumulation of CLP and enhanced freeze tolerance measured by amount of ion leakage and rate of photosynthetic electron transport upon freezing. These antifreeze proteins genes will be good candidates for transformation into crops such as lettuce and strawberry to develop into the new crops capable of freeze-storage and such as rose and grape to enhance a freeze tolerance for a safe survival during winter.