• Advances in nano research
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• v.17 no.1
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• pp.61-73
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• 2024

This paper presents nonlinear vibration analysis of a composite cylindrical shell. The core of the shell is made of functionally graded (FG) porous materials and layers is fabricated of carbon nanotubes (CNTs) reinforced nanocomposites. To increase the accuracy of results, neutral surface position is considered. First-order shear deformation theory is used as displacement field to derive the basic relations of equation motions. In addition, von-Karman nonlinear strains are employed to account geometric nonlinearity and to enhance the results' precision, the exact position of the neutral surface is considered. To governing the partial equations of motion, the Hamilton's principle is used. To reduce the equation motions into a nonlinear motion equation, the Galerkin's approach is employed. After that the nonlinear motion equation is solved by multiple scales method. Effect of various parameters such as volume fraction and distribution of CNTs along the thickness directions, different patterns and efficiency coefficients of porous materials, geometric characteristics and initial conditions on nonlinear to linear ratio of frequency is investigated.

• The Journal of the Korean Society for Microbiology
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• v.17 no.1
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• pp.75-85
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• 1982

In order to demonstrate the effect of specific serum IgG antibody on the adherence of Streptococcus mutans to smooth surface and the mechanism of effective adherence inhibition by IgG antibody, in the present study authors obtained purified IgG from different immunogen preparations of S. mutans NCTC 10449(serotype c) and observed the effect of each IgG preparation on the adherence of each S. mutans strain cultured in different conditions. In addition, the present study was undertaken to observe the cross-reactivity of IgG and the effect of sucrose concentration on the adherence of S. mutans in vitro non-growth condition. The adherence of S. mutans to glass surface was effectively inhibited by serum IgG antibody. At the same IgG concentrations, anti-2% fructose grown/1N NaCl washed S. mutans NCTC 10449 cell showed greater adherence inhibitory effect to S. mutans strains than anti-2% sucrose grown and anti-S. mutans NCTC 10449 cell wall, and the greater inhibitory effects of IgG preparations were observed in assay using 2% fructose grown S. mutans cell preparations than using 0.1% sucrose grown cell preparations. These results suggest that the more effective adherence inhibition by serum IgG antibody is due to the reaction with S. mutans cell surface antigens rather than glucan and cell-associated glucosyltransferase. The greatest adherence inhibitory effect of IgG to S. mutans strains was observed on homologous NCTC 10449 strain and the inhibition cross-reactivities were observed between serotype c, e, and f strains. More pronounced cross-reactivity of adherence inhibition of IgG to S. mutans was observed in assay using anti-2% fructose grown/1N NaCl washed cell than using other IgG preparations, and observed in assay using 2% fructose grown S. mutans cell preparations than 0.1% sucrose grown cell preparations. It was interested that low, but adequate concentration of reactive IgG antibody significantly increased the adherence ability of S. mutans. This result may be due to the formation of small cell aggregates resulted in a increase in the numbers of organisms which adhered to glass surface. The adherence of S. mutans to glass surface was possible in the absence of glucan-synthetic activity. Low level of sucrose significantly increased the adherence ability of S. mutans to glass surface, but excessive amount of sucrose induced large cell aggregates resulted in a decrease in the numbers of organism which adhered.

• International Journal of Highway Engineering
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• v.10 no.2
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• pp.47-55
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• 2008

In this paper, comparisons are presented on the predicted tensile strains which can affect the fatigue life of a thin asphalt concrete (AC) pavement near the surface of pavement from three-dimensional (3D) finite element (FE) using 3D measured tire contact stresses of a radial tire and a bias ply tire and a layered linear elastic program (BISAR). The objective was to analyze the stress distributions for a 11R22.5 radial tire and a $10{\times}20$ bias ply tire, and to compare the predicted tensile strains at the top and bottom of AC surface using different analysis methods. The results show that the stress distributions of two tires are similar but the 11R22.5 radial tire has much higher vertical contact stress than that of the $10{\times}20$ bias ply tire. The predicted tensile strains at the bottom of AC layer under the center of tire showed higher value by BM (BISAR with the measured contact area) method, which the measured tire contact area is used in a layered elastic program, while the tensile strain at the top of AC surface of 3.5cm offset distance from tire edge by 3D FE analysis showed the highest values among three analysis methods.

• International Journal of Highway Engineering
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• v.6 no.4 s.22
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• pp.109-121
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• 2004

This study was performed to investigate the characteristics of drying shrinkage for concrete slabs as a project for Korean pavement design procedure. According to the volume-surface ratios and aggregate types, the experiments have been executed for 252 days. In order to simulate the volume-surface ratio of a real concrete pavement slab, three-layer epoxy coating and wrapping were used to prevent the evaporation at the part of specimen surfaces. As a result of preliminary test, coating and wrapping method was identified as reliable for three months. According to the volume-surface ratio, the drying shrinkage of the concrete specimen using sandstone was measured 1.32 to 1.8 times higher than that of the limestone specimen. Comparing to the measured drying shrinkage strains and established ACI and CEB-FIP model equations, it turned out that those model equations were underestimated. Finally, considering the age and volume-surface ratios, the prediction equations of the drying shrinkage of concrete specimen were proposed through a multiple nonlinear regression analysis.

• Mycobiology
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• v.39 no.1
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• pp.33-39
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• 2011

Wild yeasts on the surface of various fruits including grapes were surveyed to obtain yeast strains suitable for fermenting a novel wine with higher alcohol content and supplemented with rice starch. We considered selected characteristics, such as tolerance to alcohol and osmotic pressure, capability of utilizing maltose, and starch hydrolysis. Among 637 putative yeast isolates, 115 strains exhibiting better growth in yeast-peptone-dextrose broth containing 30% dextrose, 7% alcohol, or 2% maltose were selected, as well as five ${\alpha}$-amylase producers. Nucleotide sequence analysis of the 26S rDNA gene classified the strains into 13 species belonging to five genera; Pichia anomala was the most prevalent (41.7%), followed by Wickerhamomyces anomalus (19.2%), P. guilliermondii (15%), Candida spp. (5.8%), Kodamaea ohmeri (2.5%), and Metschnikowia spp. (2.5%). All of the ${\alpha}$-amylase producers were Aureobasidium pullulans. Only one isolate (NK28) was identified as Saccharomyces cerevisiae. NK28 had all of the desired properties for the purpose of this study, except ${\alpha}$-amylase production, and fermented alcohol better than commercial wine yeasts.

• Journal of Microbiology
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• v.38 no.1
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• pp.1-7
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• 2000

Eight strains of Aeromonas hydrophila isolated from diseased trout in Korea were characterized and compared with an American type strain by various methods including biochemical and physiological tests, PCR, randomly amplified polymorphic DNA (RAPD), plasmid profiling, and gel electrophoresis of total, membrane, and extracellular proteins. Virulence factors such as surface array proteins, cytotoxin, hemolysin, haemagglutinin, and protease were also investigated. The Korean strains showed heterogeneity in Iysine decarboxylase production, utilization of various carbon sources, and production of acetoin. Five strains had the same profiles of total and membrane proteins. Six strains haemagglutinated with trout red blood cells (RBCs) which was inhibited by fucose, galactose, and mannose, except for No. 1 where haemagglutination was inhibited by only galactose and mannose, but not by fucose. Four isolates haemagglutinated with human RBCs which was inhibited by fucose and mannose yet not by galactose. The type strain haemagglutinated only with trout RBCs which was inhibited by fucose, galactose, and mannose. Every isolate secreted protease, hemolysin, cytotoxin, and siderophore, but no enterotoxin. Results showed that the Korean isolates, except for No.7, had very different biochemical and molecular characteristics from those of the American type strain.

• Journal of the Optical Society of Korea
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• v.7 no.2
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• pp.106-112
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• 2003

In order to do continuous health monitoring of large structures, it is necessary that the distributed sensing of strain and temperature of the structures be measured. So, we present the temperature compensation of a signal from a fiber optic BOTDA (Brillouin Optical Time Domain Analysis) sensor. A fiber optic BOTDA sensor has good performance of strain measurement. However, the signal of a fiber optic BOTDA sensor is influenced by strain and temperature. Therefore, we applied an optical fiber on the beam as follows: one part of the fiber, which is sensitive to the strain and the temperature, is bonded on the surface of the beam and another part of the fiber, which is only sensitive to the temperature, is located nearby the strain sensing fiber. Therefore, the strains can be determined from the strain sensing fiber while compensating for the temperature from the temperature sensing fiber. These measured strains were compared with the strains from electrical strain gages. After temperature compensation, it was concluded that the strains from the fiber optic BOTDA sensor had good coincidence with those values of the conventional electrical strain gages.

• Journal of Species Research
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• v.13 no.1
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• pp.10-31
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• 2024

In this study, 11 strains of epilithic cyanobacteria belonging to six unrecorded species in Korea were isolated from gravel submerged in freshwater of seven collection sites in Korea. The morphological characteristics of the six species isolated in this study were consistent with the type strain of each species, and the similarity of the 16S rRNA gene sequences with the type strain of each species were 98.8-100%. In the phylogenetic tree using the 16S rRNA gene sequences, the 11 strains of these six species formed the same cluster as the strains of each species. The habitat of each previously reported species is mainly the soil surface, but all Korean strains appeared from the gravel submerged in freshwater. As a result of the morphological, ecological, and molecular analyses, these six species of cyanobacteria were identified as Geminocystis papuanica, Allocoleopsis franciscana, Ancylothrix terrestris, Klisinema persicum, Scytolyngbya timoleontis, and Shackletoniella antarctica, which were added as newly recorded species in Korea.

• ALGAE
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• v.39 no.2
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• pp.59-74
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• 2024

Terrestrial cyanobacteria are extremely diverse. In urban areas, they can be found as black stains on the surface of building walls, stone monuments, or man-made structures. Many of the terrestrial cyanobacteria are still understudied. To expand knowledge of terrestrial cyanobacterial diversity, a polyphasic characterization was performed to identify 12 strains isolated from campus of University of the Ryukyus, Okinawa, Japan. Multigene phylogenetic analyses based on 16S rRNA gene and 16S-23S rRNA internal transcribed spacer (ITS) region showed that the isolated strains formed two independent subclades within Nodosilinea, and were distantly related to all described Nodosilinea species. The 16S-23S rRNA ITS secondary structures showed variations for D1-D1' and Box B domain, while V3 domain was almost identical among entire species of Nodosilinea, including the studied strains. In addition, a unique morphological character, i.e. forming nodule or spiral shape, was also observed in certain studied strains. According to polyphasic characterization, Nodosilinea coculeatus sp. nov. and Nodosilinea terrestrialis sp. nov., were proposed as two new species of terrestrial cyanobacteria from Okinawa.

• Korean Journal of Microbiology
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• v.27 no.3
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• pp.250-258
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• 1989

S. mutans known as a causative causative agent of dental caries was isolated from a carious lesion of Korean in the present study. The physiological, biochemical characteristics and polysaccharide pattern of these isolates were compated with those of four laboratory strains ; AHT(a), FA-1F(b), LM7(e), and OMZ65(g). One hundred strains of oral streptococci were isolated from dental caries sites of Korean (one male and one female). Among these, 3 strains were identified as S. mutans. These strains were able to grow on selective media MS, MST, MSP, MSP1, MSB, MSBT and were stained dark pink when sprayed with solutions of mannitol and TTC. So, these strains were called strain 108, 110, and 120, respectively. Strain 108, 110, and 120 were bacitracin resistnt. As these strains contained particularly hippurate hydrolysis enzyme, they were distinguished from laboratory strains. Apart from laboratory strains, the strain 108 was not capable of fermenting lactose, the strain 110 was not able to ferment sorbitol, inulin, melibiose, raffinose and the strain120 was incapable of fermenting inulin, raffinose. All fractions of extracellular and ecll bound polysaccharide of the strain108, 110, and 120 were consisted of more glucan than fructan. Aside from laboratory strains, the isolated strains were composed of more water-insoluble glucans related adherence on solid surface than water-soluble. According to these results, the strain108, 110, and 120 had native characteristecs of S. mutans, but they were different from laboratory strains in some characteristics. Therefore, each of them was given a name to S. mutans KHC108, KHC110, and KHC120, respcetively.