• Title/Summary/Keyword: surface sterilization

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Nonthermal Sterilization of Dried Laver by Intense Pulsed Light with Batch System (회분식 광펄스 처리에 의한 마른 김의 비가열 살균)

  • Kim, Ae-Jin;Shin, Jung-Kue
    • Korean Journal of Food Science and Technology
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    • v.46 no.6
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    • pp.778-781
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    • 2014
  • Intense pulsed light (IPL) is a nonthermal technology emerging as an alternative to conventional thermal treatment. The purpose of this study was to investigate the effect of IPL treatment on the microbial inactivation, color alteration, and temperature change of dried laver to evaluate the commercial feasibility of IPL as a sterilization method. IPL treatment (10 min at 1,000 V and 5 pps) resulted in approximately 1.6 log CFU/g decrease in microbial cell viability. After IPL treatment, the surface temperature of dried laver increased by $1.9^{\circ}C$. The color lightness of dried laver increased with increased treatment time, while redness and yellowness decreased. However, these color differences were not significant.

Electrochemical Desalination of a 50% w/w Sodium Hydroxide Solution, a Pharmaceutical Sterilization Agent

  • Jaehong Lee;Ji-hyun Yang;Eugene Huh;Sewon Park;Bonmoo Koo;Ik-Sung Ahn
    • Journal of Electrochemical Science and Technology
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    • v.14 no.1
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    • pp.59-65
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    • 2023
  • Sodium hydroxide solutions are often employed as sterilization agents in the pharmaceutical industry. Here, the chloride content is considered as a critical impurity. In this study, an electrochemical method was developed to remove chloride ions (Cl-) through the oxidative deposition of AgCl on a Ag anode. The Cl- content in the commercially available 50% w/w NaOH solution employed was approximately 100 mg Cl-/kg NaOH. As the OH- content is approximately 18,000 times higher than the Cl- content, the formation of AgCl may be expected to be thermodynamically less favorable than the formation of Ag2O. However, activation energies for AgCl and Ag2O formation have been reported to be approximately 3.8 and 31.2 kJ/mol, respectively, and indicate that AgCl formation is favored. AgCl can be selectively produced by controlling the anode potential. Here, the Cl- concentration was reduced to less than 50 mg Cl-/kg NaOH when an anode potential of 0.18 or 0.19 V vs. Hg/HgO (reference electrode) was applied for one hour at 50℃. XRD analysis and visual monitoring of the Ag anode confirmed the oxidative deposition of AgCl on the anode surface as well as the electrochemical desalination of the concentrated NaOH solution.

Sterilization effect of electrolyzed water and chlorine dioxide on Rubus coreanus Miquel (전기분해수와 이산화염소수 처리 복분자(Rubus coreanus Miquel)의 살균효과)

  • Teng, Hui;Kim, You Ho;Lee, Won Young
    • Food Science and Preservation
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    • v.20 no.4
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    • pp.459-466
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    • 2013
  • This study was carried out in order to investigate sterilization effect and to extend storage periods of the Rubus coreanus by treating with tap water (TW), electrolyzed water (EW) and aqueous chlorine dioxide ($ClO_2$). After each treatment plot was soaked with 10, 50, 100, 200 ppm in each sterilizing solution within 30 sec, each treatment was compared during the storage time at room temperature and refrigerator temperature. As results of total plate count according to temperatures and periods, the microbial sterilizing power of each treatment plot was bigger at EW and $ClO_2$ treatment plots than the TW treatment plot; however, it sharply increased on the high concentration $ClO_2$ treatment plot. Futhermore, the cold storage treatment plot had more outstanding microbial sterilizing power than the room temperature treatment plot. As a result of observing the surface of the Rubus coreanus using scanning electron microscope (SEM), no microbe was seen in EW and $ClO_2$ treatment plot. The results of measuring enzyme activity showed a more significant decrease in EW and $ClO_2$ solutions treatment plot than TW treatment plot but gradually increased with time. The contents of total polyphenol revealed similar values on each treatment. The EW and $ClO_2$ treatment of the Rubus coreanus could be considered as good methods for inhibiting microbial growth in fresh vegetables and fruit, thereby contributing to quality maintenance.

Development of an advanced atmospheric pressure plasma source with high spatial uniformity and selectiveness for surface treatment

  • Im, Yu-Bong;Choe, Won-Ho;Lee, Seung-Hun;Han, U-Yong;Lee, Jong-Hyeon;Lee, Sang-Gyun;Ha, Jeong-Min;Kim, Jong-Hun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2016.02a
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    • pp.176-177
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    • 2016
  • In the last few decades, attention toward atmospheric pressure plasma (APP) has been greatly increased due to the numerous advantages of those applications, such as non-necessity of high vacuum facility, easy setup and operation, and low temperature operation. The practical applications of APP can be found in a wide spectrum of fields from the functionalization of material surfaces to sterilization of medical devices. In the secondary battery industry, separator film has been typically treated by APP to enhance adhesion strength between adjacent films. In this process, the plasma is required to have high stability and uniformity for better performance of the battery. Dielectric barrier discharge (DBD) was usually adopted to limit overcurrent in the plasma, and we developed the pre-discharge technology to overcome the drawbacks of streamer discharge in the conventional DBD source which makes it possible to produce a super-stable plasma at atmospheric pressure. Simulations for the fluid flow and electric field were parametrically performed to find the optimized design for the linear jet plasma source. The developed plasma source (Plasmapp LJPS-200) exhibits spatial non-uniformity of less than 3%, and the adhesion strength between the separator and electrode films was observed to increase 17% by the plasma treatment.

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A Study of Seawater Sterilization for Ballast Water using solution plasma (수중방전에 의한 선박평형수 처리에 관한 연구)

  • Choe, Jong-Beom;Yang, Jeong-Hyeon;Kim, Yeon-Won;Gang, Jun;Lee, Myeong-Hun;Yun, Yong-Seop
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2017.05a
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    • pp.141-141
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    • 2017
  • 국제해사기구(International Maritime Organization)는 선박 평형수로 인하여 발생하는 환경문제를 방지하기 위하여 지난 2004년 선박 평형수 관리 협약을 제정하였고, 2016년 핀란드의 비준함에 따라 2017년 9월 8일부터 발효될 예정이다. 이 협약에 대응하기 위하여 선박 평형수 처리기술이 개발되어 승인이 되었다. 이중 수중방전을 이용한 기술은 화학약품의 첨가 등이 없어 경제적이고 내부에서 발생하는 OH라디컬, 자외선, 충격파, 전기장, 오존 등에 다양한 요소들에 의하여 수처리가 가능하고, 중화를 위한 첨가제 등이 필요없어 친환경적이다. 본 연구에서는 부산 항만 근처연안에서 채취한 바닷물을 대상으로 수중방전을 이용한 방법을 연구하였다. 실험방법은 그림1과 같이 Point to Point 방식의 전극을 꾸몄고, 용액은 1L이며, 전압은 8kV, 30kHZ로 가해주었다. 광학분석장비(OES)를 이용하여 수중방전시 발생되는 빛 분석을 하였다. 실험결과 OES분석 결과 살균작용하는 OH 라디컬이 발생하는 것을 확인할 수 있었다. 또한 실험 도중 시료를 채취하여 $35^{\circ}C$ 48시간동안 배양하였다. 배양 결과 시간이 경과함에 따라 미생물의 개체가 그림 2와 같이줄어두는 것을 확인할 수 있었다.

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Biodegradation Characteristics of Poly(butylene succinate-co-butylene adipate) during Soil Burial Test (토양 매립 시험에서 Poly(butylene succinate-co-butylene adipate)의 생분해 특성)

  • Kim, Mal-Nam
    • Korean Journal of Environmental Biology
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    • v.28 no.3
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    • pp.150-157
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    • 2010
  • Biodegradation behavior of poly(butylene succinate-co-butylene adipate) (PBSA) was examined when PBSA was buried in the natural soil and the soil inoculated with Burkholderia cepacia after sterilization. After 80 days of the soil burial test at room temperature, the PBSA film buried in the natural soil lost 34.0% of its intial weight, while the same film lost 59.2% of its initial weight when buried in the sterile soil inoculated with B. cepacia. The optical and SEM observations of the surface morphology of the PBSA film also indicated that the surface erosion and rupture took place faster when the film was buried in the sterile soil inoculated with B. cepacia compared to the film buried in the natural soil. Viable cell number in the natural soil and that the sterile soil inoculated with B. cepacia increased by a factor of 6~7 and 10~14, respectively as compared to the initial viable cell number.

An Ozone Micro-bubble Technique for Seed Sterilization in Alfalfa Sprouts

  • Kwack, Yurina;Kim, Kyoung Koo;Hwang, Hyunseung;Chun, Changhoo
    • Horticultural Science & Technology
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    • v.32 no.6
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    • pp.901-905
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    • 2014
  • The efficacy of ozone micro-bubble water (OMBW) in reducing microbial populations on alfalfa seeds was investigated in this study. We observed the surface of alfalfa seeds using microscopy and found that many cracks and crevices existing on the surface could harbor pathogens. Alfalfa seeds were treated with tap water (TW), micro-bubble water (MBW), ozone water (OW), ozone micro-bubble water (OMBW), and chlorine water (CL) for 5 min, and total microbial population, E. coli and Salmonella spp. colonies were determined. Also, the sterilized seeds were germinated and cultivated for 5 d after sowing to investigate the percentage of germination and the growth of alfalfa sprouts. The treatments with OMBW and CL were most effective in reducing total microbial populations and E. coli was eliminated by OW, OMBW, and CL treatments. CL treatment reduced the percentage of germination and fresh weight of alfalfa sprouts, but OMBW did not cause any negative effects on the germination and growth of alfalfa sprouts. These results indicate that OMBW can be used as an effective sanitizer for eliminating seed-borne pathogens without detrimental effects on seed viability.

High Power Factor Electronic Ballast for Ultraviolet Germicidal Lamp (자외선 살균 램프용 고역률 전자식 안정기에 관한 연구)

  • Kang, Bum-Suk;Kim, Hee-Jun
    • Proceedings of the KIEE Conference
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    • 2000.07b
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    • pp.1202-1205
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    • 2000
  • Germicidal lamps efficiently emit a large amount of ultraviolet rays 253.7nm which have excellent germicidal effect. The lamps are primarily useful for sterilization of air, the surface of various materials and water or liquid. In this paper, analysis of the charge pump power factor correction inverter for driving a 65W UV lamp and electrical characteristics of the lamp are discussed. The operation of the inverter circuit. in which the lamp is included as a load, is analyzed. Experimental results of the inverter circuit are also presented.

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First Report of Anthracnose Disease Caused by Colletotrichum gloeosporioides in Stonecrop

  • Jeon, Chang Wook;Kwak, Youn-Sig
    • Journal of agriculture & life science
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    • v.50 no.5
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    • pp.251-255
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    • 2016
  • In year of 2013, anthracnose disease symptoms were observed on stem of stonecrop in a plant resource field, Southern Forest Resource Research Center, Jinju, South Korea. The disease symptoms were appeared 2-5 cm smudged irregular black spot color on the plant stems. Suspected pathogen was isolated from the lesion with surface sterilization method and identified it as Colletotrichum gloeosporioides by morphological characteristics and phylogenetic analyses. Artificially inoculated the isolated pathogen led to the similar disease symptom as originally observed in the field. The fungal pathogen was re-isolated from the inoculated lesion and it exhibited the same characteristics compared to the original isolate. This is the first report of anthracnose of stonecrop grown in open fields, caused by Colletotrichum gloeosporioides in Korea.

A Study on How to Kill Airborne Bacteria and Viruses in Elementary Schools (초등학교내 공기중 부유세균 및 바이러스 사멸방법에 대한 연구)

  • Lee, Su Yeon;Kim, Chang Soo;Kwak, Eun Mi;Im, Jong Eon;Jeon, Jae Hwan;Kwon, Jun Ho
    • Journal of the Society of Disaster Information
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    • v.18 no.3
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    • pp.566-573
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    • 2022
  • Purpose: This study attempted to verify the effectiveness of the application of air sterilizers in elementary schools at risk of group infection among vulnerable groups in order to address fears of new infectious diseases that have increased since the outbreak of Middle East Respiratory Syndrome (MERS) and Coronavirus infection-19 (COVID-19). Method: One air sterilizer was installed in each classroom, cafeteria, and bathroom of an elementary school in Seoul, and surface and air samples were collected at a distance of 2m from the air sterilizer, and the bacterial reduction effect was analyzed compared to the uninstalled control group. Result: The sterilization effect on the surface was less than 2log CFU/cm2 in both the control group and the test group, and the test group showed 54 to 87% less general bacterial colony formation than the control group. In addition, the sterilization effect in the air differed depending on the location of the air sterilizer, and the wall installation showed a reduction effect of up to 91% compared to the control group, and the central installation showed a reduction effect of up to 93%. Conclusion: As a result of the study, it is expected that the prevention of infectious diseases can be increased by maintaining the current quarantine program in elementary schools that conduct regular disinfection while applying air sterilizers. In addition, it is considered desirable to facilitate the inflow of air into the air sterilizer.