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Characterization and Antimicrobial Resistance of Vibrio parahaemolyticus Strains Isolated from Seawater of Geum River Estuary Area, West Coast of Korea (금강 하구 해역의 해수에서 분리한 장염비브리오(Vibrio parahaemolyticus) 균의 특성 및 항균제 내성)

  • Lee, Shin-Hye;Kim, Hee-Dai;Park, Kwon-Sam
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.6
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    • pp.850-857
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    • 2022
  • Seventy-five Vibrio parahaemolyticus isolates from the surface seawater of the Geum River Estuary area, on the west coast of Korea, were analyzed for the presence of virulence genes and susceptibility to 17 different antimicrobials. All 75 isolates were examined for the presence of two virulence genes (tdh or trh) using polymerase chain reaction; Only one of the isolates possessed the tdh or trh gene. According to the results of disk diffusion susceptibility tests, all of the strains were resistant to penicillin G, 92.0% were resistant to ampicillin, 82.7% were resistant to amoxicillin, 2.7% were resistant to ciprofloxacin, 2.7% were resistant to trimethoprim, 1.3% were resistant to cephalothin, and 1.3% were resistant to erythromycin. However, all of the strains were susceptible to amikacin, cefoxitin, chloramphenicol, gentamycin, kanamycin, nalidixic acid, nitrofurantoin, rifampin, streptomycin, and tetracycline. The average minimum inhibitory concentrations for ampicillin for V. parahaemolyticus was 557.6 ㎍/mL. These results not only provide novel insight into the necessity for seawater sanitation in Geum river estuary area, but they help reduce the risk of contamination of antimicrobial-resistant bacteria.

Temporal and Spatial Variation of Microalgal Biomass and Community Structure in Seawater and Surface Sediment of the Gomso Bay as Determined by Chemotaxonomic Analysis (색소분석을 통한 곰소만 내 해수와 퇴적물 중 미세조류 생체량과 군집구조의 시공간적 변화)

  • Lee, Yong-Woo;Park, Mi-Ok;Yoon, Ji-Hyun;Hur, Sung-Bum
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.17 no.2
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    • pp.87-94
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    • 2012
  • To compare monthly variations of phytoplankton biomass and community composition between in seawater and sediment of the Gomso Bay (tidal flat: approximately 75%), the photosynthetic pigments were analyzed by HPLC every month in 1999 and every two months in 2000. Ambient physical and chemical parameters (temperature, salinity, nutrients, dissolved oxygen, and chemical oxygen demand) were also examined to find the environmental factors controlling structure of phytoplankton community. The temporal and spatial variations of chlorophyll a concentration in seawater were correlated well with the magnitude of freshwater discharge from land. The biomass of microphytobenthos at the surface sediments was lower than that in other regions of the world and 2-3 times lower than phytoplankton biomass integrated in the seawater column. Based on the results of HPLC pigment analysis, fucoxanthin, a marker pigment of diatoms, was the most prominent pigment and highly correlated with chlorophyll a in seawater and sediment of the Gomso Bay. These results suggest that diatoms are the predominant phytoplankton in seawater and sediment of the Gomso Bay. However, the monthly variation of chlorophyll a concentration in seawater at the subtidal zone was not a good correlation with that in sediment of the Gomso Bay. Although pelagic plankton was identified in seawater by microscopic examination, benthic algal species were not found in the seawater. These results suggest that contribution from the suspended microphytobenthos in the tidal flat to the subtidal zone of the Gomso Bay may be low as a food source to the primary consumer in the upper water column of the subtidal zone. Further study needs to elucidate the vertical and horizontal transport magnitude of the suspended microphytobenthos in the tidal flat to the subtidal zone.

Evaluation of Simple CO2 Budget with Environmental Monitoring at an Oyster Crassostrea gigas Farm in Goseong Bay, South Coast of Korea in November 2011 (2011년 11월 고성만 굴(Crassostrea gigas) 양식장 수질환경 모니터링을 통한 이산화탄소 수지 평가)

  • Shim, JeongHee;Ye, Miju;Lim, Jae-Hyun;Kwon, Jung-No
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.6
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    • pp.1026-1036
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    • 2014
  • Real-time monitoring for environmental factors (temperature, salinity, chlorophyll-a, etc.) and fugacity of carbon dioxide ($fCO_2$) was conducted at an oyster Crassostrea gigas farm in Goseong Bay, south coast of Korea during 2-4th of November, 2011. Surface temperature and salinity were ranged from $17.9-18.7^{\circ}C$ and 32.7-33.8, respectively, with daily and inter-daily variations due to tidal currents. Surface $fCO_2$ showed a range of $390-510{\mu}atm$ and was higher than air $CO_2$ during the study period. Surface temperature, salinity and $fCO_2$ are showed significant correlations with chl.-a and nutrients, respectively. It means when chl.-a value is high in surface water of the oyster farm, active biological production consume $CO_2$ and nutrients from environments and produce oxygen, suggesting a tight feedback between biological processes and environmental reaction. Thus, factors affecting the surface $fCO_2$ were evaluated using a simple mass balance. Temperature and biological productions by phytoplankton are the main factors for $CO_2$ drawdown from afternoon to early night, while biological respiration increases seawater $CO_2$ at night. Air-sea exchange fraction acts as a $CO_2$ decreasing gear during the study period and is much effective when the wind speed is higher than $2-3m\;s^{-1}$. Future studies about organic carbon and biological production/respiration are required for evaluating the roles of oyster farms on carbon sink and coastal carbon cycle.

Corrosion Characteristics of Reinforced Steel Bar Emedded in Multiple Mortar Specimen(W/C:0.5) Aged 5 Years in Seawater

  • Moon, Kyung-Man;Takeo, Oki;Won, Jong-Pil;Park, Dong-Hyun;Kim, Yun-Hae
    • International Journal of Ocean System Engineering
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    • v.3 no.1
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    • pp.33-37
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    • 2013
  • Reinforced concrete structures have been increasingly widely used in numerous industrial fields. These structures are often exposed to severely corrosive environments such as seawater, contaminated water, acid rain, and the seashore. Thus, the corrosion problems that occur with the steel bars embedded in concrete are very important from the safety and economic points of view. In this study, the effects of the cover thickness on the corrosion properties of reinforced steel bars embedded in multiple mortar test specimens immersed in seawater for 5 years were investigated using electrochemical methods such as the corrosion potentials, polarization curves, cyclic voltammograms, galvanostat, and potentiostat. The corrosion potentials shifted in the noble direction, and the value of the AC impedance also exhibited a higher value with increasing cover thickness. Furthermore, the polarization resistance increased with increasing cover thickness, which means that the oxide film that is deposited on the surface of a steel bar surrounded by alkali environment exhibits better corrosion resistance because the water, chloride ions and dissolved oxygen have difficulty penerating to the surface of the steel bar with increasing cover thickness. Consequently, it is considered that the corrosion resistance of reinforced steel can be improved by increasing the cover thickness. However, the corrosion resistance values of a steel bar estimated by measuring the corrosion potential, impedance and polarization resistance were not in good agreement with its corrosion resistance obtained by polarization curves.

Contamination Status and Characteristics of Persistent Organochlorine Pesticides in the Saemangeum Environment (새만금 해양환경에서의 잔류성 유기염소계 농약의 분포 및 특성)

  • Hong, Sang-Hee;Shim, Won-Joon;Li, Dong-Hao;Yim, Un-Hyuk;Oh, Jae-Ryoung;Kim, Eun-Soo
    • Ocean and Polar Research
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    • v.28 no.3
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    • pp.317-329
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    • 2006
  • To assess the contamination status of organochlorine pesticides in the marine environment of Saemangeum, surface seawater and sediments were collected and analyzed. Organochlorine pesticides were widely distributed in the Saemangeum environment, with DDT, HCH and endosulfan II contamination being particularly prevalent. The overall concentrations of DDTs, HCHs, and endosulfan II in surface seawater were in the rage of 0.03-0.65ng/l (mean value: 0.36ng/l), 1.31-4.54ng/l (2.63 ng/l), and not detected (ND)-0.96ng/l (0.49ng/l), respectively. Among the target organochlorine pesticides, endosulfan II showed the hi띤est level at the river mouth flowing into the Saemangeum. Additionally, its negative gradient from the rivers to the outside and a good correlation with $NO_3^-$ indicate that endosulfan ll inflows to the Saemangeum through the river; this conclusion is also supported by the elevated levels of endosulfan II in sediments at the river mouth. In sediment, the concentration of DDTs, HCHs, CHLs, and endosulfan II were in the range of 0.05-1.96 ng/g, 0.03-0.31ng/g, 0.01-0.13ng/g, and ND-0.62ng/g, respectively. Levels of contamination in Saemangeum are relatively lower than the median concentrations previously observed at the Korean coastal regions and sediment quality guidelines suggested by NOAA (Long et at. 1995).

Evaluation of Butyltin Compounds and its Distribution Among Seawater, Sediment and Biota from the Kwangyang Bay (광양만내 유기주석화합물의 해수, 퇴적물, 생물중 농도 및 분포 상관관계)

  • KIM Gue Yoong;PARK Mi-Ok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.4
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    • pp.291-298
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    • 2001
  • Seawater, sediment and biota in the Kwangyang Bay were analyzed by gas chromatography/quartz furnace atomic absorption spectroscopy (GC-QFAAS) to investigate concentrations and distribution pattern of butyltin compounds (TBT, DBT, MBT) during February, April and July, 1996, Marine biota analyzed were Tapes japcnicus and Crassostrea gigas. The concentrations of tributyltin (TBT) in seawater were in the range of ND-15.7 ng/L for the surface and ND-68.5 ng/L for the bottom. The highest concentration of TBT in seawater was detected in April for the both, surface and bottom water. The maximum value of $TBT_{(bottom)}/TBT_{(surface)}$, 3.6 in April showed the increased input of TBT from the surface water in April compared to February (2.1) and July (0.9). The concentrations of TBT in the sediment were in the range of ND-8.5 ng/g dry wt. The highest concentration of TBT in the sediment was measured in July, This result seems to attributed to the removal of TBT from water column via sorption onto particulate matters to the relatively undisturbed underlying sediment and increased input of TBT by increased fluxes of detritus of marine plankton after spring bloom, in July. The mean values of partitioning coefficient ($K_d$) of TBT between seawater and sediment were $3.0\times10^3$(February), $7.4\times10^3$(April) and $9.4\times10^3$(July). The concentrations of TBT in biosamples were in the range of ND-93.30 ng/g dru wt. (T. japonicus) and ND-138.53 ng/g dry wt. (C. gigas). The seasonal variation of TBT contents in biota was remarkable. The $K_d$ (biological concentration factor) was $7-41\times10^3$ for T. japonicus. and $5-34\times10^3$ for C. gigas. The measured TBT concentrations in seawater in the study area was sufficient to cause the imposex of shellfish and to retard the growth of aquatic organisms including oyster upon chronic exposure.

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Weathering and Degradation Assessment of Rock Properties at the West Stone Pagoda, Gameunsaji Temple Site, Korea

  • Lee, Chan Hee;Lee, Myeong Seong;Kim, Jiyoung
    • Conservation and Restoration of Cultural Heritage
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    • v.1 no.1
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    • pp.29-37
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    • 2012
  • The West Stone Pagoda at Gameunsaji Temple Site constructed in the 7th century is mainly composed of dark grey dacitic tuff bearing small numerous dioritic xenoliths. These xenoliths resulted in small holes due to differential weathering process from the host rocks. Physical strength of the pagoda was decreased due to weathering and damage caused by petrological, biological and coastal environmental factors. The southeastern part of the pagoda was extremely deteriorated that the rock surface showed exfoliation, fracture, open cavity, granular decomposition of minerals and salt crystallization by seawater spray from the eastern coast. The stone blocks were intersected by numerous cracks and contaminated by subsequent material such as cement mortar and iron plates. Also, the pagoda was colonized by algae, fungi, lichen and bryophytes on the roof rock surface and the gaps between the blocks. As a result of ultrasonic test, the rock materials fell under Highly Weathered Grade (HW) or Completely Weathered Grade (CW). Thus, conservational intervention is essentially required to prevent further weakening of the rock materials.

Carbon nanospheres synthesized via solution combustion method: their application as an anode material and catalyst for hydrogen production

  • Dhand, Vivek;Rao, M. Venkateswer;Prasad, J.S.;Mittal, Garima;Rhee, Kyong Yop;Kim, Hyeon Ju;Jung, Dong Ho
    • Carbon letters
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    • v.15 no.3
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    • pp.198-202
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    • 2014
  • Amorphous agglomerates of carbon nanospheres (CNS) with a diameter range of 10-50 nm were synthesized using the solution combustion method. High-resolution transmission electron microscopy (HRTEM) revealed a densely packed high surface area of $SP^2$-hybridized carbon; however, there were no crystalline structural components, as can be seen from the scanning electron microscopy, HRTEM, X-ray diffraction, Raman spectroscopy, and thermal gravimetric analyses. Electrochemical and thermo catalytic decomposition study results show that the material can be used as a potential electrode candidate for the fabrication of energy storage devices and also for the production of free hydrogen if such devices are used in a fluidized bed reactor loaded with the as-prepared CNS as the catalyst bed.