• Korean Chemical Engineering Research
• /
• 제58권2호
• /
• pp.280-285
• /
• 2020

We have developed a constitutive display system of the Pseudomonas fluorescens SIK W1 TliA lipase on the cell surface of Escherichia coli using E. coli outer membrane protein C (OmpC) as an anchoring motif, which is an economical compared to induced system. For the constitutive expression of truncated OmpC-TliA fusion proteins, gntT104 promoter was employed. Cell growth was not affected by over expression of fusion protein during entire culture time, suggesting cell lysis was not a problem. The localization of truncated OmpC-TliA fusion protein on the cell surface was confirmed by immunofluorescence microscopy and measuring whole cell lipase activity. Constitutively displayed lipase was very stable, retaining activity enantioselectivity throughout the five repeated reactions. These results suggest that OmpC from E. coli be a useful anchoring motif for displaying enzymes on the cell surface without any inducers, and this stable surface display system can be employed for a broad range of biotechnological applications.

• Journal of the Optical Society of Korea
• /
• 제15권3호
• /
• pp.305-309
• /
• 2011

We present an efficient design approach for a plasmonic slot waveguide using a genetic algorithm. The analyzed structure consists of a nanometric slot in a thin metallic film embedded within a dielectric. To achieve high confinement without long propagation length, the thickness and width of the slot are optimally designed in order to optimize the figures of merit including mode confinement and propagation length. The optimized design is based on the finite element method and enhances the guiding and focusing of light power propagation.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 1999년도 하계학술대회 논문집 B
• /
• pp.969-971
• /
• 1999

Wavelet analysis is applying to many fields such as the time-frequency localization of a time series and a time varying data. In this paper, a statistical testing based Wavelet power spectrum analysis for the stationary Nino3 Sea Surface Temperature(SST) data was executed. Specially, the 95% confidence level for SST was effective in searching the periods of El-Nino using various wavelet basis functions.

• 한국산학기술학회논문지
• /
• 제21권5호
• /
• pp.69-76
• /
• 2020

수중에서 방사된 음원의 신호는 해수면, 해저면 반사에 의한 다중경로 시간지연(multi-path time-delay)을 포함하여 체계(system)의 센서에 수신된다. 다중경로 환경과 복잡한 해양환경의 외란(disturbance)에 의해 수신된 신호간 상관성(coherence)이 저하된다. 따라서, 신호간 시간지연값을 이용하여 수중음원의 위치를 추정하는 체계는 추정성능의 저하가 나타난다. 이러한 환경에서도 강인한 음원 위치 추정성능을 위해 선배열(uniform line array), 평면배열(rectangular array)과 같은 다양한 형태의 센서배열과 빔형성(beamforming) 기법, 비용함수(cost-function)과 같은 신호처리를 이용하여 왔다. 본 연구에서는 선배열 형태의 doublet array와 추정된 시간지연값 보정 기법을 이용하고자 한다. 3 개의 doublet array를 동일 선에 위치하였으며 각각의 doublet array에 수신된 신호간의 시간지연값을 2 단계로 추정하였다. 수신신호에 대해 상호상관(cross-correlation) 함수를 적용하여 추정된 시간지연값을 수신신호의 중심주파수(center-frequency)와 array의 개구경(aperture) 및 수중음원과의 기하학적 관계에 따라 보정하여 최종의 시간 지연값을 얻었으며 이로부터 음원의 거리와 방위를 추정하였다. 제안한 기법의 타당성을 확인하기 위해, Monte-Carlo method를 이용하여 시뮬레이션하였다.

• 한국공작기계학회논문집
• /
• 제12권5호
• /
• pp.34-39
• /
• 2003

The paper will present chip formation mechanism and surface integrity generation mechanism based on the systematical experimental tests. Some basic factors such as the end milling cutter tooth number, cutting forces, cutting temperature, cutting vibration the chip status, the surface roughness, the hardness distribution and the metallographic texture of the machined surface layer are involved. The chip formation mechanism is typical thermal plastic shear localization at high cutting speed with less number of shear ribbons and bigger shear angle than that at low speed, which means lack of chip deformation. The high cutting speed with much more cutting teeth will be beneficial to the reduction of cutting forces, enlarge machining stability mot depression of temperature increment anti-fatigability as well as surface roughness. The burrs always exist both at low cutting speed and at high cutting speed. So the deburring process should be arranged for milling titanium alloy in my case.

• 한국동물학회지
• /
• 제35권2호
• /
• pp.149-160
• /
• 1992

Using the protein A-gold complex, the mvoabrillogenesis and actin localization of cultured myoblast were invastisated. In the superstructural changes of mvogenic cell during differentiation, pectoral myoblasts contained large nucleus and numerous ribosomes but no myofibrils during the first 24 hr of cultures. Mvoblast initiated to differentiate at 3-day of culture contained the primitive myofibrillar structure. At 96 hr of culture, the mvofibrillar structure showed reletively discernable Z band but pools defined A, H and M bands. The feature of sarcomeric structure showed more defined form at cultur 5 day. In the aspect of actin localization, actin wvas diffusely detected throughout the cytoplasm of myogenic cell and nucleus during the proliferating stage. At 72 hr of culture, with the appearantc oi primitive mvofibrils, gold particles were observed in surrounding of myofibrils but still presented in overall of cytoplasm, especially in the surface and lumen of endoplasmic reticulum. With the gradual increase of culture time, local distribution of actin was readily detected within cytoplasm. In the 5-day specimen of cultures, gold particles precisely indicate the sites of actin localifation within the sarcomere. These results indicate the time of onset of myofibrill appearance and the biosynthetic and incorporation pathway of actin molecules into sarcomeric structure during myofibrillogenesis. Thus, in the present study, the first mvoabrillar structure was detected at culture 3 day, and the initiation of assembly into a typical sarcmeric structure was observed at culture 5 day. It seems, however, that the course of events on myofibrillogenesis of cultured myoblasts can be changed with great dependence of culture conditions including the number and groluth rate of mononucleated mvoblasts after seeding although the fundamental process shows identical appearances.

• 한국산학기술학회논문지
• /
• 제16권11호
• /
• pp.7773-7780
• /
• 2015

팝퍼 케틀의 국산화 개발을 위하여 기존 케틀을 분석하고, 열선 배치에 따른 열전달 해석을 통하여 케틀 국산화 개발을 위한 연구를 수행하였다. 케틀 재료 분석을 위한 시편을 제작하고 Polishing 및 Etching을 수행하였다. SEM을 이용하여 시편의 표면을 관찰하여 페라이트 퍼얼라이트 재질을 디프 드로잉 방식으로 제작하였음을 파악하였다. 도금층 재질 및 두께 분석을 통해 Ni(16%)도금이 $16{\sim}49{\mu}m$임을 파악하였다. 또한 열선 배치에 따른 열전달 특성을 파악하고, 최적 열선 시스템을 개발하였다. 케틀의 장시간 작업 시간 중에 발생하는 케틀 과열 시 작동을 중지하는 제어 시스템을 제작하였다. 케틀 개발 이후 성능 평가를 수행하여 부피팽창률에 대한 평가 규격 KS G3602를 만족하였다.

• 대한원격탐사학회지
• /
• 제37권5_1호
• /
• pp.1163-1175
• /
• 2021

자율 주행에서 도로 표시는 객체 추적, 경로 계획을 위한 필수 요소이며 측위를 위한 중요한 정보를 제공할 수 있다. 이 논문은 역 관점 매핑 이미지와 정밀도로지도 투영 이미지를 매칭하여 비교함으로써 도로 노면 표지의 갱신 및 위치 측위를 하는 접근 방식을 제시한다. 역 관점 매핑(IPM; Inverse Perspective Mapping) 기법을 사용하여 차량의 전방을 촬영한 카메라 이미지에서 원근 효과를 제거하고 2D 도메인으로 버드뷰 이미지를 생성한다. 그 다음에 GNSS/INS를 참조하여 촬영된 이미지와 동일한 정밀도로지도 영역을 이미지로 생성하여 두 이미지의 노면표지가 최대한 일치하도록 피팅 한다. 또한 정지선, 횡단 보도, 점선 및 직선 등 문자와 화살표를 인식하여 정밀도로지도의 객체와 비교 함으로써 갱신 여부를 판단 한다. 그리고 새로 설치된 객체의 측위는 주변의 객체의 위치 좌표값을 정밀도로지도에서 참조하여 얻을 수 있다. 우리는 매우 낮은 계산 비용과 저가의 카메라 및 GNSS/INS 센서 만으로도 빠르게 갱신된 정밀도로지도를 얻을 수 있다.

• 한국소음진동공학회논문집
• /
• 제21권12호
• /
• pp.1120-1131
• /
• 2011

Researches using time reversal acoustics(TRA) for impact localization have been paid attention to recently. Dispersion characteristics of Lamb waves, which restrict the utility of classical nondestructive evaluation based on time-of-flight information, can be compensated through the application of TRA to Lamb waves on a plate. This study investigates the spatial focusing performance of time reversal Lamb waves on a plate using finite element analysis. In particular, the virtual sensor effect caused by multiple wave reflections at the boundaries of a plate is shown to enable the spatial focusing of Lamb waves though a very small number of surface-bonded piezoelectric(PZT) sensors are available. The time window size of forward response signals, are normalized with respect to the number of virtual active sensors. Then their effects on the spatial focusing performance of Lamb waves are investigated.

• 한국생산제조학회지
• /
• 제22권3호
• /
• pp.452-458
• /
• 2013

Over the past several years, many studies have been carried out in the field of 3D data inspection systems. Several attempts have been made to improve the quality of manufactured parts. The introduction of laser sensors for inspection has made it possible to acquire data at a remarkably high speed. In this paper, a robust inspection technique for detecting defects in 3D pressed parts using laser-scanned data is proposed. Point cloud data are segmented for the extraction of features. These segmented features are used for shape matching during the localization process. An iterative closest point (ICP) algorithm is used for the localization of the scanned model and CAD model. To achieve a higher accuracy rate, the ICP algorithm is modified and then used for matching. To enhance the speed of the matching process, aKd-tree algorithm is used. Then, the deviation of the scanned points from the CAD model is computed.