• Title/Summary/Keyword: surface display

Search Result 1,681, Processing Time 0.034 seconds

Display of Bacillus macerans Cyclodextrin Glucanotransferase on Cell Surface of Saccharomyces cerevisiae

  • Kim, Kyu-Yong;Kim, Myoun-Dong;Han, Nam-Soo;Seo, Jin-Ho
    • Journal of Microbiology and Biotechnology
    • /
    • v.12 no.3
    • /
    • pp.411-416
    • /
    • 2002
  • Bacillus macerans cyclodextrin glucanotransferase (CGTase) was expressed on the cell surface of Saccharomyces cerevisiae by fusing with Aga2p linked to the membrane-anchored protein, Aga1p. The surface display of CGTase was confirmed by immunofluorescence microscopy and its enzymatic ability to form ${\alpha}$-cyclodextrin from starch. The maximum surface-display of CGTase was obtained by growing recombinant S. cerevisiae at $20^{\circ}C$ and pH 6.0. S. cerevisiae cells displaying CGTase on their surface consumed glucose and maltose, inhibitory byproducts of the CGTase reaction, to enhance the purity of produced cyclodextrins. Accordingly, the experimental results described herein suggest a possibility of using the recombinant S.cerevisiae anchored with bacterial CGTase on the cell surface as a whole-cell biocatalyst for the production of cyclodextrin.

Optical Properties Control by Surface Treatment on Display Cover Glass (디스플레이 커버 글라스의 표면 처리에 의한 광학요소 제어)

  • Kim, Sung Soo;Hwang, Jai Suk;Jeon, Bup Ju
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
    • /
    • v.28 no.9
    • /
    • pp.607-614
    • /
    • 2015
  • To provide the clear images from the direct light on electrical board and display devices, anti glare treatment of display cover glass is needed. In this study, the effects of surface treatment temperature, concentration, and etching solution coating thickness of the gel phase on optical elements control such as gloss, haze of reflected light and transmittance, were investigated. Cover glasses were treated at different coating thickness and additive concentration. The optical properties were examined using spectrophotometer, gloss and haze meter. The surface morphology and roughness were measured by the optical microscope and Ra measuring instrument. The etching rate and surface morphologies were dramatically affected by the concentration of acid additive in the viscous gel because of re-crystallization of components in the etching solution, hydrogel formation and coagulant after coating on glass substrate. In our experimental range, cover glass which is surface-treated with various optical properties as well as the morphology uniformity was obtained; in particular, optical properties could be controlled by etching solution coating thickness of the gel phase and the concentration of additive. The gloss was depended on the surface roughness and it showed the linear relationship between optical transmittance and haze of reflected light, respectively.

Numerical Simulation on Dispersion of Fume Micro-Particles by Particle Suction Flows in Laser Surface Machining (입자 석션유동에 따른 레이저 표면가공의 마이크로 흄 오염입자 산포 특성 해석연구)

  • Kyoungjin Kim
    • Journal of the Semiconductor & Display Technology
    • /
    • v.22 no.4
    • /
    • pp.1-6
    • /
    • 2023
  • In CO2 laser surface machining of plastic films in modern display manufacturing, scattering of fume particles could be a major source of well-recognized film surface contamination. This computational fluid dynamics research investigates the suction air flow patterns over a film surface as well as the dispersion of micron-sized fume particles with low-Reynolds number particle drag model. The numerical results show the recirculatory flow patterns near laser machining point on film surface and also over the surface of vertical suction slot, which may hinder the efficient removal of fume particles from film surface. The dispersion characteristics of fume particles with various particle size have been tested systematically under different levels of suction flow intensity. It is found that suction removal efficiency of fume particles heavily depends on the particle size in highly nonlinear manners and a higher degree of suction does not always results in more efficient particle removal.

  • PDF

NOVEL SURFACE PROFILER SYTSTEM FOR INSPECTION OF FLAT PANEL DISPLAY

  • Kimura, Munehiro;Akahane, Tadashi;Iwata, Tetsuya
    • 한국정보디스플레이학회:학술대회논문집
    • /
    • 2004.08a
    • /
    • pp.325-327
    • /
    • 2004
  • Novel surface profiler system for inspection of the display components is demonstrated In the case of the liquid crystal display, for example, not only the flatness of the alignment film but also the quality of rubbing can be inspected. Furthermore, the shape of the component such as the color filter, electrode and mirror can be inspected without removing each component.

  • PDF

Long period application of AC field causing contrast reduction of IPS cell

  • Momoi, Yuichi;Koda, Tomonori
    • 한국정보디스플레이학회:학술대회논문집
    • /
    • 2008.10a
    • /
    • pp.870-872
    • /
    • 2008
  • It is known that AC electric field to drive IPS mode of liquid crystal display (LCD) causes reduction of the contrast after long period of display operation. Our results are presented in this paper on a base of a model of interaction between surface of PI alignment film and LC molecules. We conclude that the surface viscosity of PI alignment film is one of the most important factors for the contrast reduction phenomenon.

  • PDF

Effects of Process Induced Damages on Organic Gate Dielectrics of Organic Thin-Film Transistors

  • Kim, Doo-Hyun;Kim, D.W.;Kim, K.S.;Moon, J.S.;KIM, H.J.;Kim, D.C.;Oh, K.S.;Lee, B.J.;You, S.J.;Choi, S.W.;Park, Y.C.;Kim, B.S.;Shin, J.H.;Kim, Y.M.;Shin, S.S.;Hong, Mun-Pyo
    • 한국정보디스플레이학회:학술대회논문집
    • /
    • 2007.08b
    • /
    • pp.1220-1224
    • /
    • 2007
  • The effects of plasma damages to the organic thin film transistor (OTFT) during the fabrication process are investigated; metal deposition process on the organic gate insulator by plasma sputtering mainly generates the process induced damages of bottom contact structured OTFTs. For this study, various deposition methods (thermal evaporation, plasma sputtering, and neutral beam based sputtering) and metals (gold and Indium-Tin Oxide) have been tested for their damage effects onto the Poly 4-vinylphenol(PVP) layer surface as an organic gate insulator. The surface damages are estimated by measuring surface energies and grain shapes of organic semiconductor on the gate insulator. Unlike thermal evaporation and neutral beam based sputtering, conventional plasma sputtering process induces serious damages onto the organic surface as increasing surface energy, decreasing grain sizes, and degrading TFT performance.

  • PDF

Modeling an e-Beam Addressed Liquid Crystal Projection Display

  • Yang, Deng-Ke;Zhou, Fushan;Molitor, R.J.
    • 한국정보디스플레이학회:학술대회논문집
    • /
    • 2002.08a
    • /
    • pp.930-932
    • /
    • 2002
  • We have carried a theoretical study on e-beam addressed liquid crystal projection display in which the liquid crystal is switched by the electric field of the charge, produced by an electron beam, on the surface of the display. We calculated the electric field produced by the surface charge, the liquid crystal director configuration and the profile of the transmitted light. We studied the factors affecting the resolution of the display. We also studied the effect of pretilt angle on the performance of the display. The e-beam addressed liquid crystal projection display potentially has the advantages of high resolution and high brightness.

  • PDF

Quantification of the Scum on the Black Matrix Surface of Color Filter for LCD (LCD용 칼라필터의 Black Matrix 표면에 발생하는 잔사의 정량화)

  • Koo, Young-Mo;Lee, Jong-Seo;Yi, Choong-Hoon
    • Analytical Science and Technology
    • /
    • v.12 no.5
    • /
    • pp.415-420
    • /
    • 1999
  • We estimated the quantity of the scum remaining on the Black Matrix (BM) surface of color filter. To do this, histogram was analyzed which was obtained from AFM image of the BM surface. We divided the histogram to two Gaussian functions of the free BM surface (1) and the scum (2), and calculated the areas ($a_1$, $a_2$) of both the Gaussian functions. We quantified the residue as the ratio of the area ($a_2/(a_1+a_2)$). As a result of the Gaussian functions of the free BM surface, it was revealed that another kind of residue remained on the BM surface. It was difficult to quantify it. but it could relatively be estimated from the average height and the standard deviation.

  • PDF

Novel Bacterial Surface Display System Based on the Escherichia coli Protein MipA

  • Han, Mee-Jung
    • Journal of Microbiology and Biotechnology
    • /
    • v.30 no.7
    • /
    • pp.1097-1103
    • /
    • 2020
  • Bacterial surface display systems have been developed for various applications in biotechnology and industry. Particularly, the discovery and design of anchoring motifs is highly important for the successful display of a target protein or peptide on the surface of bacteria. In this study, an efficient display system on Escherichia coli was developed using novel anchoring motifs designed from the E. coli mipA gene. Using the C-terminal fusion system of an industrial enzyme, Pseudomonas fluorescens lipase, six possible fusion sites, V140, V176, K179, V226, V232, and K234, which were truncated from the C-terminal end of the mipA gene (MV140, MV176, MV179, MV226, MV232, and MV234) were examined. The whole-cell lipase activities showed that MV140 was the best among the six anchoring motifs. Furthermore, the lipase activity obtained using MV140 as the anchoring motif was approximately 20-fold higher than that of the previous anchoring motifs FadL and OprF but slightly higher than that of YiaTR232. Western blotting and confocal microscopy further confirmed the localization of the fusion lipase displayed on the E. coli surface using the truncated MV140. Additionally the MV140 motif could be used for successfully displaying another industrial enzyme, α-amylase from Bacillus subtilis. These results showed that the fusion proteins using the MV140 motif had notably high enzyme activities and did not exert any adverse effects on either cell growth or outer membrane integrity. Thus, this study shows that MipA can be used as a novel anchoring motif for more efficient bacterial surface display in the biotechnological and industrial fields.

The effect of 3-mercapto-5-nitro-benzimidazole (MNB) and poly (methyl methacrylate) (PMMA) treatment sequence organic thin film transistor

  • Park, Jin-Seong;Suh, Min-Chul;Jeong, Jong-Han;Kim, Su-Young;Mo, Yeon-Gon
    • 한국정보디스플레이학회:학술대회논문집
    • /
    • 2006.08a
    • /
    • pp.1174-1177
    • /
    • 2006
  • A bottom contact organic thin film transistor (OTFT) is fabricated with an organic double-layered gate insulator (GI) and pentacene. The PMMA and MNB layers are treated on gate insulator and source/drain (S/D, Au) before depositing pentacene to investigate device properties and pentacene growth. The sequence of surface treatment affects a device performance seriously. The ultra-thin PMMA (below 50A) was deposited on organic gate insulator and S/D metal by spin coating method, which showed no deterioration of on-state current (Ion) although bottom contact structure was exploited. We proposed that the reason of no contact resistance (Rc) increase may be due to a wettability difference in between PMMA / Au and PMMA / organic GI. As a result, the device treated by $PMMA\;{\rightarrow}\;MNB$ showed much better Ion behavior than those fabricated by $MNB\;{\rightarrow}\;PMMA$. We will report the important physical and electrical performance difference associated with surface treatment sequence.

  • PDF