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Display of Bacillus macerans Cyclodextrin Glucanotransferase on Cell Surface of Saccharomyces cerevisiae  

Kim, Kyu-Yong (Department of Food Science and Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University)
Kim, Myoun-Dong (Department of Food Science and Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University)
Han, Nam-Soo (Department of Food Science and Technology, Chungbuk National University)
Seo, Jin-Ho (Department of Food Science and Technology, Chungbuk National University)
Publication Information
Journal of Microbiology and Biotechnology / v.12, no.3, 2002 , pp. 411-416 More about this Journal
Abstract
Bacillus macerans cyclodextrin glucanotransferase (CGTase) was expressed on the cell surface of Saccharomyces cerevisiae by fusing with Aga2p linked to the membrane-anchored protein, Aga1p. The surface display of CGTase was confirmed by immunofluorescence microscopy and its enzymatic ability to form ${\alpha}$-cyclodextrin from starch. The maximum surface-display of CGTase was obtained by growing recombinant S. cerevisiae at $20^{\circ}C$ and pH 6.0. S. cerevisiae cells displaying CGTase on their surface consumed glucose and maltose, inhibitory byproducts of the CGTase reaction, to enhance the purity of produced cyclodextrins. Accordingly, the experimental results described herein suggest a possibility of using the recombinant S.cerevisiae anchored with bacterial CGTase on the cell surface as a whole-cell biocatalyst for the production of cyclodextrin.
Keywords
CGTase; surface display; Saccharomyces cerevisiae; whole-cell catalyst;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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