• International Journal of Oral Biology
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• 제39권4호
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• pp.187-192
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• 2014

Nonthermal atmospheric pressure plasma has attracted great interest for biomedical applications. The plasma consists of charged particles, radicals, and a strong electric field as the fourth state of matter. This study evaluated the change in the surface roughness after tooth bleaching by plasma in combination with a low concentration (15%) of carbamide peroxide, specifically whether the application of plasma produced detriments, such as demineralization and structural change, with the goal of efficient and safe tooth bleaching. After being combined with plasma and 15% carbamide peroxide, the hydroxyapatite surface was significantly smoother with a low roughness average value. Tooth bleaching with 15% carbamide peroxide alone produced an irregular surface and increased the surface roughness with high roughness average value. Tooth bleaching with plasma resulted in no significant variations in hydroxyapatite in terms of change in surface roughness and surface topography. The application of tooth bleaching with plasma is not deleterious to dental hard tissue, implicating it as a safe tooth bleaching technique.

• Anatomy and Cell Biology
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• 제57권2호
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• pp.183-193
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• 2024

The corona of the glans clitoris is a clinically important yet poorly understood anatomical structure. There has been longstanding confusion regarding the prevalence of the corona of the glans clitoris and, moreover, its very existence. Therefore, this anatomical study assesses the prevalence of the corona of the glans clitoris and the gross anatomy of the proximal glans clitoris. Anatomy was assessed in 104 female donor bodies ranging in age from 50 to 102 years with an average age-at-death of 78.1±10.9 years (mean±SD). All clitorises (100%; 104:104 dorsums and 100%; 208:208 sides) were found to have a well-defined clitoral corona. Three of 104 (2.9%) coronas possessed grossly visible, outward-projecting, bluntly rounded papillae. Some donors possessed a coronopreputial frenulum. Clitoropreputial adhesions were common and associated with clitoral pearls. Clitoral pearls were identified in 37.8% (14:37) of unembalmed donors and observed to create clitoral craters, structural deformations in the surface of the corona and glans. The results of this study suggest that the corona of the glans clitoris is a ubiquitous anatomical structure. The clitoral coronal papillae and coronopreputial frenulum are novel, previously undescribed, anatomical structures. This study identifies that the corona of the glans clitoris is prone to pathological processes such as clitoral pearl formation and clitoral deformation. In addition to novel anatomical findings, the results of this study call attention to the need for life-long clitoral examinations. Furthermore, the corona of the glans clitoris should be regularly included in anatomical texts and accurately depicted in anatomical illustrations.

• Anatomy and Cell Biology
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• 제51권4호
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• pp.302-304
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• 2018

Prior knowledge of arterial supply to the head and neck is of substantial importance for well-planned surgeries involving the concerned region. We are reporting an unusual and rare variation in the branching pattern of external carotid artery in a 60-year-old female cadaver. A common trunk known as thyrolinguofacial trunk, originating from the anterior surface of the external carotid artery (right and left) giving of superior thyroid artery and a linguofacial trunk during a routine neck dissection. The linguofacial trunk then divided into a lingual and a facial artery. Vascular abnormalities are usually detected either on the dissection table or by the radiologists during imaging or accidently during surgeries leading to serious consequences.

• Applied Microscopy
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• 제14권2호
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• pp.81-93
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• 1984

Structural differences in various divisions of the rabbit colon were investigated using light and scanning electron microscopy. For light microscopic study, various Portions of the colon from seven rabbits (2.5 kg body weight) were fixed in 10% neutral buffered formalin, and paraffin sections were stained with hematoxylin-eosin. Tissues for scanning electron microscopy were fixed in 1% glutaraldehyde-1.5% paraformaldehyde and postfixed in 1% $OsO_4$, dehydrated to 100% alcohol, transfered to isoamilacetate and dried by the critical point method. Subsequently, specimens were coated with gold and viewed with a JSM-35C scanning electron microscope. The colon displays a morphological diversity along its proximo-distal axis. Five regions can be discerned based on the macroscopic and microscopic characteristics. 1) The first segment immediately distal to the cecocolical junction possessing three teniae is approximately 5 cm ($4{\sim}6cm$) in length, and displays irregular folds of the mucosa oriented transversely similar to those of the cecum. 2) The second segment possessing three teniae is about 7 cm ($5{\sim}8cm$) in length, and is characterized by the papilla-like protrusions on the mucosal surface. 3) The third segment, possessing a single tenia is about 16 cm ($12{\sim}20cm$) in length, and also displays the papilla-like protrusions similar to the aforegoing segment. 4) Fusus coli, approximately 4 cm ($3{\sim}5cm$) in length, is free of teniae and exhibits longitudinal folds on the mucosal surface. These four portions together constitute the proximal colon. 5) The distal colon reaches a length of about 58 cm ($53{\sim}55cm$) and shows a pattern of surface irregularities with minor ridges on the mucosal folds.

• 대한정형외과학회지
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• 제54권5호
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• pp.393-401
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• 2019

견관절 주위 통증은 정형외과 의사가 외래에서 접하는 가장 흔한 문제 중 하나이다. 견관절 주위 통증을 치료하기 위한 여러 치료 방법 중 상견갑신경 차단술뿐만 아니라 관절 및 관절 주위 주사 요법은 좋은 임상적 결과를 보이고 있다. 초음파 유도하 주사요법은 안전한 테크닉으로 합병증을 줄이고 시술의 안전성과 정확도를 높이고 있다. 초음파 유도하 견관절 주위 주사요법을 하기 위해서는 표면 해부학을 정확히 숙지하는 것이 중요하다. 이 논문은 표면해부학에 대한 설명 및 견관절과 주위 구조물의 초음파 해부학을 기술하는 데 그 목적이 있다. 또한 여러 주사방법 및 신경 차단술에 대해 설명하고자 한다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2006년도 춘계학술대회 논문집
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• pp.177-178
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• 2006

Many authors issued the feature-preserving averaging technique according to positioning and scaling process using landmarks, which represent the geometric characteristics of three dimensional surface models. Such a technique should be done by manual procedure, choosing and marking the landmarks on each bone surface before averaging process. In this study, we produced another averaging technique without having to use such manual procedure, and made averaging models from three dimensional surface data that were reconstructed from computerized tomography images of Digital Korean Project. The bone models were subjected to orthogonal coordinator system. These models were transformed to coincide mass center and to align principal axis. Then, bone models were scaled according to average length data of sample bone models on all axis(x, y, z). After establishing voxellar hexahedron space which contain all sample bone models, we counted the number of overlapping for each voxel. We generated the three dimensional average surface by displaying the yokels that have more overlapping number than boundary number. The boundary number was decided when the average volume of each bone equal to the volume of bone that would be averaged. Using this technique, we can make a feature-preserving averaging volume of bones.

• IMMUNE NETWORK
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• 제11권6호
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• pp.390-398
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• 2011

Background: Epstein Barr virus (EBV) infected B cells are transformed into lymphoblastoid cell lines. Some researchers suggested some a few similarities between this process and carcinogenesis. We observed the expression of CD80 and CD86, co-stimulatory molecules on EBV-transformed B cells and changes of CD54 expression after stimulation of CD80 and CD86. Methods: CD80 and CD86 were stimulated using anti-CD80 and anti-CD86 monoclonal antibodies. To assess apoptosis and surface protein expression, flow cytometric analysis was performed. Intracellular signal molecules were evaluated by RT-PCR and immunoblot. Morphology and localization of proteins were examined using inverted or confocal microscope. Results: Cross-linking of CD80 and CD86 induced apoptosis and interfered with proliferation of EBV-transformed B cells, and dispersion of clumped cells. We also examined that their stimulation induced ROS accumulation and reduced CD54 expression. Interestingly, we observed that CD80 and CD86 diminished the expression of CD54 in different methods. Both CD80 and CD86 downregulated activation of focal adhesion kinase. CD80 stimulus inhibited CD54 expression through mainly RhoA inactivation, while CD86 down-regulated Ras and JNK phosphorylation. Conclusion: These results suggest that co-stimulatory CD80 and CD86 molecules, expressed EBV-transformed B cells, may play a role in apoptosis and cell adhesion.

• 대한치과의사협회지
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• 제9권1호
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• pp.59-62
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• 1971

An electromyographic analysis of the buccinator muscle was undertaken, by use of surface electrode. Electromyograms were made from 30 subjects with normal dentition during various oral activity, The results were as follows; 1. No buccinator activity was elicited during slowly opening and closing the mouth. 2. The majority of subjects showed buccinator activity while biting hard, protracting the mandible, and retracting the mandible. 3. The buccinator muscle was found to be markedly and consistently active during swallowing, blowing, sucking and masticating. 4. The buccinator activity during pulling the lips against the teeth was of equal potential with pulling the lips laterally. 5. The buccinator muscle on the working side was more active than the buccinator muscle on the balancing side.

• 한국전자현미경학회:학술대회논문집
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• 한국현미경학회 2001년도 제32차 추계학술대회
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• pp.43-44
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• 2001

• Applied Microscopy
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• 제50권
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• pp.15.1-15.6
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• 2020

Sample preparation including dehydration and drying of samples is the most intricate part of scanning electron microscopy. Most current sample preparation protocols use critical-point drying with liquid carbon dioxide. Very few studies have reported samples that were dried using chemical reagents. In this study, we used hexamethyldisilazane, a chemical drying reagent, to prepare plant samples. As glandular trichomes are among the most fragile and sensitive surface structures found on plants, we used Millingtonia hortensis leaf samples as our study materials because they contain abundant glandular trichomes. The results obtained using this new method are identical to those produced via critical-point drying.