• Title/Summary/Keyword: sulII-strA-strB-tetR-tetA cluster

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Organization of Antibiotic Resistance Gene Cluster of Multi-Drug Plasmid in Clinically Isolated Salmonella Enteritidis Strain (식중독환자에서 분리한 Salmonella Enteritidis 다제내성 플라스미드의 내성유전자 집락의 구조해석)

  • Jung, Seo-Yeon;Son, Chang-Kyu;Kwak Kyung-Tak;Kim, Byung-Chun;Park, Wan
    • Korean Journal of Microbiology
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    • v.38 no.4
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    • pp.299-305
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    • 2002
  • Clinically isolated Salmonella Enteritidis strain has a multi drug resistance plasmid, which confers ampicillin, chloramphe-nicol, sulfonamide, streptomycin and tetracycline, named pCAST2. We cloned a 7 kb Sacl fragment of pCAST2 which has sulfonamide, streptomycin and tetracycline resistance genes. The 7 kb SacI fragment showed the organization of sulII-strA-strB-tetR-tetA gene cluster which is different from the other clusters reported previously. In this study, we presented the method to detect this cluster by PCR analysis and showed that this cluster was found in Salmonella strains occurred sporadically at Kyungpook province in 2002.