• 생명과학회지
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• 제10권6호
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• pp.591-597
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• 2000

An investigation was conducted to elucidate the effect of sucrose, germicides and abscisic acid (ABA) in the preservatives on postharvest quality, such as fresh weight, solution uptake, vase life, diameter and thickness of flower, and chlorolhyll of leaf in cut chrysanthemum 'Kyoungsubang`. Fresh weight of cut chrysanthemum was highest in 250mg/L aluminum sulfate [{TEX}$Al_{2}(SO_{4})_{3}${/TEX}]+3% sucrose and 250 mg/L 8-hydroxyquinoline sulfate (8-HQS)+ 3% sucrose. Addition of sucrose and germicides inhibited microorganisms growth in solution, and apparently promoted solution uptake. The increased fresh weight seems to attribute to the elevated uptake. Germicide 8-HQS was more effective than {TEX}$Al_{2}(SO_{4})_{3}${/TEX} in the aspect of solution uptake and fresh weight. Treatment of 250 mg/L 8-HQS +3% sucrose showed the longest vase life (24.67±2.52 days) and the lowest rate of leaf chlorosis (or senescence). Addition of {TEX}$Al_{2}(SO_{4})_{3}${/TEX} and sucrose accelerated leaf chlorosis and increased diameter of flower, respectively. Sucrose treatment prolonged the vase life of flowers. Except control and 250 mg/L 8-HQS, content of chlorophyll was rapidly decreased after 6 days at upper, middle and lower leaves in order.

• 한국미생물·생명공학회지
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• 제32권3호
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• pp.199-205
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• 2004

Bifidobacterium longum SJ32 균주로부터 cloning한 sucrose phosphorylase 유전자를 포함하는 8.6 kb의 EclRI 단편의 염기서열을 결정하였다. 5개의 open reading frame이 존재하였고, 상동성 검색의 결과, sucrose대사에 관여하는 sucrose phosphorylase (ScrP), sucrose transporter (ScrT), GalR-LacI-type transcriptional regulator (ScrR) 유전자의 존재를 확인하였다. SJ32 균주의 scrPTR 유전자군은 다른 B. longum균주의 scrPTR과 배열이 동일하고, 각 유전자 산물이 아미노산 수준에서 94%이상의 상동성을 가지고 있지만, 주변 유전자는 다르게 나타나 B. longum균주 간의 scrPTR 유전자군의 horizontal transfer를 추정하게 한다. 대장균에서의 scrP와 scrT의 동시 발현은 세포 내로의 sucrose 유입을 증가시켜 sucrose phosphorylase의 활성 증가에 영향을 주는 것으로 나타나, scrT가 sucrose transporter유전자임을 뒷받침한다. 기존에 보고된 B. longum NCC2705균주의 유전체로부터 scrPTR외에도 sucrose대사에 관여하는 다양한 sucrose multiple transport system에 관여하는 유전자의 존재가 확인되어, B. longum이 다양한 sucrose유입체계를 보유하고 있음이 추정된다.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.88-89
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• 2002

Traditionally, kinetics of brain influx of drugs has been evaluated by a number of experimental techniques. Brain uptake index and in situ brain perfusion study have been used for the determination of the kinetics; However, these methods generally focus on the accuracy of the uptake rate into the brain rather than the speed of the determination. In addition, application of radiolabelled substrates (e.g., $_{14}$C-labelled sucrose) further impedes the wide spread acceptance of these techniques for the application of high throughput screening system. (omitted)

• 식물조직배양학회지
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• 제27권1호
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• pp.47-50
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• 2000

당류의 수송속도는 당류 고갈동안 증가되었고, 당류 고갈 3일째에 최대 수송속도를 보였다. 당류 고갈로 유도된 당류 수송계는 cycloheximide에 의해서 완전히 방해되었다 선천적으로 식물에서 당류 수송계는 하나이나 당류 고갈동안 유도된 포도당 수송은 포화상태를 보이고, 설탕 수송은 포화되지 않았다. 유도된 당류 수송계는 선천적인 당류 수송계와는 다른 kinetic을 보였다. 이 결과는 고등식물도 불리한 조건하에서 새로운 수송계를 유도시킬 수 있는 능력이 있음을 보여주었다.

• 동아시아식생활학회지
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• 제5권1호
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• pp.101-107
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• 1995

Staphylococcus aureus, the most salt-tolerant food-borne pathogen, produces enterotoxins which may cause symptoms such as vomiting, diarrhea, nausea, and cramps. Since this bacterium has been able to grow at extremely high osmolarity its identity in foods with low water activity values such as salted or dried foods is of great concern. In this study, the growth of S. aureus at high osmolarity has been studied and the doubling time of S. aureus grown at TSB medium containing 15% NaCl has been found to be increased to 4∼5 hours. The stimulation of proline uptake after exposure of cells to high concentration of both extracellular KCl and sucrose was not increased. Stimulation of proline uptake at these environment only occured when 25mM NaCl was present I transport buffer. In additional experiments, the time required to reach mid-logarithmic phase in defined medium of high osmolarity found to be reduce by the presence of glycine betaine, proline, and choline.

• Archives of Pharmacal Research
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• 제10권2호
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• pp.80-87
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• 1987

To get a better insight into the exxistence and the role of a Na-Ca exchange mechanism in smooth muscle, the effect of Na substitution with sucrose on tension development, cellular Ca uptake and $^{45}Ca$ efflux was investigated using isolated cat ileal longitudinal muscle strips. Experimental results were summarized as follows;1) Exposure of the cat ileal longitudinal muscle to Na-free solution induced a contraction, and the magnitude of the contraction increased after incubation of the muscle strips with ouabain ($2{\times10^{-}5}$M) for 1hr. 2) Cellular Ca uptake in Na-free solution increased with an increase in Na content of the Na-loading media, and a linear relationship existed between tissue Na content and cellular Ca uptake for 10 min 3) After tissues were equilibrated in PSS containing $^{45}Ca$ for 2hr, cellular Ca uptake decreased with rising the external Na concentration. 4)Removal of medium Na or inhibition of the Na-K pump decreased the rate of $^{45}Ca$ efflux. These results strongly suggested that Na substitution increases cellular Ca uptake and decreases the rate of $^{45}Ca$ efflux via a Na-Ca exchange mechanism.

• 원예과학기술지
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• 제31권3호
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• pp.269-274
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• 2013

To further our understanding of sugar accumulation in 'Shiranuhi' mandarin [(C. unshiu ${\times}$ C. sinensis) ${\times}$ C. reticulate], we investigated the patterns of sugar uptake in juice sacs exposed to different concentrations of sucrose, fructose and glucose in vitro. Data was also collected on the change in weight and shape of the in vitro juice sacs over time. Soluble solids content, sugar content and acidity content were highest at 20% sucrose, fructose and glucose solution content; while fructose content was highest at 5% sucrose concentration. Furthermore, the juice sac's fresh weight was highest at 5% sucrose and lowest at 20% fructose content. The shape of the juice sacs also differed in different sugar concentration and type. Overall, sucrose, fructose and glucose content in juice sacs increased with the sugar concentration. These results suggest that sugar translocation into juice sacs is actively induced by high sugar concentration in the medium. Thus, it can be concluded that sugar and acid accumulation in juice sacs increased with sugar concentration in vitro culture.

• Journal of Plant Biotechnology
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• 제31권2호
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• pp.169-173
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• 2004

유소직 세포들은 aldose인 D-glucose ketose인 D-fructose에 대하여 다른 능동 수송계를 소유하고 있었다. D-glucose와 D-fructose 수송계의 $K_{m}$ 값은 각각 0.28 mM과 15.02mM이었다. D-mannose는 $K_{m}$ 값이 0.44 mM로 D-glucose와 유사하였지만, 그러나 D-glucose와는 다른 수송계를 소유하고 있었다. L-glucose도 고유한 수송계를 통하여 세포내로 수송되었으며, 그러나 그 기능을 전혀 알지 못하고 있다. 유조직 원형질체는 단당류 능동 수송계만을 소유하고, 이당류인 sucrose능동 수송계는 소유하지 않고 있었다. 발달 초기단계에 있는 사부 원형질체는 glucose와 sucrose 수송계를 동시에 소유하고 있었다. 완전히 발달된 사부세포에서는 이미 존재하였던 glucose 능동 수송계는 사라지고, 새로 유도된 sucrose 능동 수송계만 존재하는 것으로 측정되었다.

• 한국잡초학회지
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• 제17권4호
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• pp.345-361
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• 1997

Photoassimilates translocate from regions of carbohydrate synthensis(source) to regions of carbohydrate utilization or storage(sink). In the source, assimilate loads into the phloem for long-distance transport. Current evidence suggests that there are twig loading mechanisms : one involves assimilate transfer via the apoplasm and then load into the phloem by carrier-mediated proton-sucrose cotransport, while the other involves movement through the continuous symplastic connections between the mesophyll cells and the phloem. Inspite of problems associated with the interpretation of experiments, the evidence for apoplastic loading remains convincing because the apoplastic loading systems explains well the observed accumulation capacity arid the selectivity of assimilate uptake by tile phloem.

• Archives of Pharmacal Research
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• 제23권4호
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• pp.391-395
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• 2000

To establish the usefulness of oxytocin (OT) as an analgesic for women in delivery, the pharmacokinetic parameters and blood-brain barrier (BBB) permeability of [$^3H$] OT were obtained using an intravenous injection technique or the internal carotid artery perfusion/capillary depletion (ICAP/CDM) method. Brain uptake of OT was similar to that of sucrose, plasma space marker, indicating that OT has a poor BBB permeability. Moreover, the analgesic effects of OT injected through the jugular vein on nociception were evaluated by the tail-flick method. The antinociceptive effects of OT injected at a dose of 0.2 ${m}g/kg$or 2 ${m}g/kg$ were dose-dependent. In addition, the analgesic effects of OT on the CNS were unaffected by naloxone, a m-receptor antagonist. In a similar manner to the opioid system, OT may play a modulatory role in antinociception.