• Journal of Microbiology and Biotechnology
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• 제24권7호
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• pp.954-958
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• 2014

Succinic semialdehyde dehydrogenase (SSADH) catalyzes the oxidation of succinic semialdehyde (SSA) into succinic acid in the final step of ${\gamma}$-aminobutyric acid degradation. Here, we characterized Bacillus subtilis SSADH (BsSSADH) regarding its cofactor discrimination and substrate inhibition. BsSSADH showed similar values of the catalytic efficiency ($k_{ca}t/K_m$) in both $NAD^+$ and $NADP^+$ as cofactors, and exhibited complete uncompetitive substrate inhibition at higher SSA concentrations. Further analyses of the sequence alignment and homology modeling indicated that the residues of catalytic and cofactor-binding sites in other SSADHs were highly conserved in BsSSADH.

• 한국미생물·생명공학회지
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• 제7권4호
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• pp.197-204
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• 1979

계육을 이용한 가공식품개발의 필요성에 의하여 원료육의 전처리 과정중 부패에 가장 큰 영향을 미치는 미생물의 증식을 억제하고자 NaOCl과 succinic acid를 병행사용하여 미생물의 증식억제 효과 및 저장기간의 연장등을 조사한 결과는 다음과 같다. 1. NaOCl의 농도증가에 따라 미생물의 증식억제효과도도 증가하였다. 200ppm 처리시 50ppm의 경우보다 1/1000로 생존균체가 더 감소하였다. 2. 탕지 처리시 6$0^{\circ}C$에서 염소의 소실도 적었으며 규체억제 및 계육표피의 보전성에서도 양호한 결과를 얻었다. 3. NaOCl에 유기산 검가시 pH 3 부근에서 급격한 균체 억제작용을 보였다. Acetic acid, succinic acid, lactic acid, citric acid 등 유기산 첨가시 succinic 효과가 pH 3.12, 0.51%의 농도에서 균체억제 효과가 가장 좋았다. 4. 200ppm NaOCl ＋0.5% succinic acid로 30분간 침지한 계육을 5$^{\circ}C$와 -18$^{\circ}C$에 명명 저장시저장기간의 연장은 무처리군보다 5$^{\circ}C$의 경우 2일간 더 연장되었다.

• 한국식품과학회지
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• 제7권2호
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• pp.74-81
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• 1975

생(生)배추, $6^{\circ}{\sim}7^{\circ}C$ 숙성(熟成)김치, $22^{\circ}{\sim}23^{\circ}C$ 숙성(熟成)김치를 ion-exchange chromatography로 총유기산(總有機酸)을 분리(分離)하고, silicic acid partition column chromatography로 각유기산(各有機酸)으로 분리(分離), 정량(定量)한 후(後) paper chromatography로 정성확인(定性確認)하였다. 생(生)배추, $6^{\circ}{\sim}7^{\circ}C$ 숙성(熟成)김치, $22^{\circ}{\sim}23^{\circ}C$ 숙성(熟成)김치에는 maleic, fumaric, lactic, succinic, malonic, oxalic, glycolic, malic, citric, tartaric acid 의 유기산(有機酸)과 $H_2SO_4$, $H_3PO_4$ 의 무기산(無機酸)이 들어 있다. 생(生)배추에는 유기산(有機酸)이 염(鹽)의 형태(形態)로 많이 들어 있으며, malic acid가 가장 많고, $6^{\circ}{\sim}7^{\circ}C$ 숙성(熟成)김치, $22^{\circ}{\sim}23^{\circ}C$ 숙성(熟成)김치에는 lactic acid+succinic acid 가 가장 많다. $6^{\circ}{\sim}7^{\circ}C$ 숙성(熟成)김치에는 $22^{\circ}{\sim}23^{\circ}C$ 숙성(熟成)김치보다 lactic acid와 succinic acid의 생성량(生成量)이 많으며, citric acid의 생성량(生成量)은 비슷하고, oxalic, malic, tartaric, fumaric, malonic, maleic, glycolic acid 의 생성량(生成量)은 적다. $6^{\circ}{\sim}7^{\circ}C$ 숙성(熟成)김치가 $22^{\circ}{\sim}23^{\circ}C$ 숙성(熟成)김치보다 더 상큼한 산미(酸味)가 많고 맛이 좋으며 이는 많이 생성(生成)된 lactic acid와 succinic acid 가 관련(關聯)이 되는 것 같다.

• 한국식품과학회지
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• 제5권4호
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• pp.231-234
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• 1973

마른김의 유기산조성(有機酸組成)과 이것의 저장중(中)의 변화(變化)를 GLC 및 GC-MS 를 사용(使用)하여 조사검토(調査檢討)하였다. 1. 마른김에 함유(含有)된 유기산(有機酸)으로서 동정(同定) 및 정량(定量)을 한 것은 pyruvic, lactic, oxalic, malonic, fumaric, succinic, malic 및 citric acid외 8종(種)이며, 양적(量的)으로 주요산(主要酸)은 oxalic, succinic, malic 및 citric acid였다. 2. oxalic, malic 및 succinic acid는 저습도하(低濕度下)에서는 큰 변화(變化)를 보이지 않으나, 고습도하(高濕度下)에서는 oxalic 및 malic acid는 급속(急速)히 감소(減少)하고, 반대(反對)로 succinic acid는 현저(顯著)히 증가(增加)하는 대조적(對照的)인 변화(變化)를 보였다. citric acid만은 저습도(低濕度)에서나 고습도(高濕度)에서나 다같이 저장기간(期間)에 따라 급속(急速)히 감소(減少)하였다. 3. 말동정성분중(末同定成分中), 그 mass spectrum으로 보아 환상구조(環狀構造)의 산(酸)이라고 추정(推定)되는 2성분(成分)은 양적(量的)으로 극히 많았으며 저장중(中)의 변화(變化)는 저습도하(低濕度下)에서는 서서(徐徐)히 감소(減少)하나, 고습도하(高濕度下)에서는 신속(迅速)히 감소(減少)하여 45일간(日間)의 저장으로 거의 소실(消失)하였다. 4. 휘발산량(揮發酸量)의 변화(變化)는 저장기간(期間)에 따라 증가(增加)하였으며, 저습도(低濕度)에서보다 고습도(高濕度)에서의 증가경향(增加傾向)이 높았다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권5호
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• pp.379-381
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• 2000

Anaerobiospirillum succiniciproducens requires expensive complex nitrogen sources such as yeast extract and polypeptone for its growth and succinic acid production. It was found that A. succiniciproducens was able to grow in a minimal medium containing glucose when supplemented with corn steep liquor (CSL) as the sole complex nitrogen source. The concentration of CSL had a significant effect on the glucose consumption by A. succiniciproducent. When 10-15 g/L of CSL was supplemented, cells were grown to an OD(sub)600 of 3.5 and produced 17.8 g/L succinic acid with 20 g/L glucose. These results are similar to those obtained by supplementing yeast extract and polypeptone, thereby suggesting that succinic acid can be produced more economically using glucose and CSL.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.531-534
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• 2000

The fumB gene encoding anaerobic fumarase of Escherichia coli XL1-Blue was introduced to solve the malic acid accumulation problem. When NZN111 harboring pTrcMLFu was cultured, 7 g/L of succinic acid was produced and malic acid was not accumulated.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권6호
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• pp.454-458
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• 2004

A study of the POSE (predispersed solvent extraction) for succinic acid by colloidalliq­uid aphrons was conducted. The organic phase contaning TOA (tri-n-octylamine) and 1-octanol permits a selective extraction of succinic acid from its aqueous solution. There was no difference of the extractability of POSE and that of conventional mixer-settler type extraction. Taking into account the no mechanical mixing in POSE, it was concluded that the POSE process is more adaptive than the conventional mixer-settler type extraction process. From mass transfer analysis at the various concentration of TOA in counter-current continuous operation, the concentration of TOA had no influence on the mass transfer coefficient. The loading values in continuous POSE were almost same as those in batch operation.

• 한국응용과학기술학회지
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• 제26권4호
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• pp.415-421
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• 2009

Esterification reaction between succinic acid[SA] and 1,4-butanediol [BD} was kinetically investigated in the presence of organic metal catalysts (alkyl-silver oxide(ASO),CAT 100E) at $150{\sim}190^{\circ}C$. The reaction rates measured by the amount of distilled water from the reaction vessel. The esterification reaction was carried out under the first order kinetics with respect to the concentration of reactants and catalyst, respectively. The overall reaction order was 2nd. From the examination of relationship between apparent reaction rate constants and reciprocal absolute temperature, the activation energy has been calculate as 146.70 kJ/mol with ASO catalyst and 43.04 kJ/mol with CAT 100E catalyst.

• 한국응용과학기술학회지
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• 제27권1호
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• pp.6-13
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• 2010

Esterification reaction between succinic acid and 1,4-butanediol was kinetically investigated in the presence of nontoxic organometallic compound catalyst(ESCAT-100E) at $150-190^{\circ}C$. The reaction rates measured by the amount of distilled water from the reaction vessel. The Esterification reaction was carried out under the first order conditions respect to the concentration of reactants, respectively. The overall reaction order was 2nd. The linear relationship was shown between apparent reaction rate constant and reciprocal absolute temperature. By the Arrhenius plot the activation energy have been calculated as 376.13 kJ/mol under nontoxic organometallic compound catalyst and also apparent reaction rate constant, k' was found to obey first kinetics with respect to the concentration of catalyst.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권5호
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• pp.347-351
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• 2001

A study was made on the extraction equilibria of succinic and formic acids from aqueous solutions using tri-n-octylamine (TOA) in 1-octanol. It was shown that the loading values of TOA decreased with increasing pH values. The apparent equilibrium constants for each acidamine complex were determined by an equilibrium model. In the case succinic acid, the formation of a bisuccinate anion played an important role in the stoichiometry of the acid-amine complex.