• Title/Summary/Keyword: subtractive

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Isolation of Differentially Expressed Genes in Bm5 Cell Line Induced with Tunicamycin for Studies of Unfolded Protein Response (UPR)

  • Kim, Sung-Wan;Yun, Eun-Young;Goo, Tae-Woo;Hwang, Jae-Sam;Kang, Seok-Woo;Kwon, O-Yu
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.10a
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    • pp.91-92
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    • 2003
  • For studies of unfolded protein response (UPR), we isolated differentially expressed genes in Bm5 cell line induced with treatment of tunicamycin, the synthesis inhibitor of N-linked oligosaccharides in cells and constructed the subtractive cDNA library enriching UPR-related genes. (omitted)

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Cloning of Epidermis-specific cDNAS Encoding a Lipid Transfer Protein and an Aldehyde Decarbonylase from Senecio odorus

  • Pyee, Jaeho
    • Journal of Plant Biology
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    • v.39 no.3
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    • pp.189-195
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    • 1996
  • The major cuticular components have been shown to be synthesized in the epidermis. Therefore, cloning of epidermis-specific genes could yield information to be used to isolate and characterize the enzymes involved in the cuticle biosynthesis. A subtractive cDNA library was prepared from Senecio odorus in which epidermis-specific cDNAs were enriched. Differential screening of the library using epidermal and non-epidermal probes revealed two cDNAs. One of them designated epi425 was identified, based on the sequence homology, as a member of a new class in the LTP gene family and the other clone designated epi23 as a gene encoding an aldehyde decarbonylase. Northern blot analyses showed that epi425 and epi23 cDNAs hybridized with a transcript of about 600 and 2, 100 nucleotides, respectively, from the epidermis but not from the non-epidermal tissues. Further characterization of these clones will provide more information on the mechanism of the cuticle biosynthesis.

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Fault Current Limiting and Recovery Characteristics's Modeling of SFCL Using Magnetically Coupled Two Coils (자기결합을 이용한 초전도 사고전류 제한기의 사고전류제한 및 회복 특성 모델링)

  • Kim, Jin-Seok;Ahn, Jae-Min;Moon, Jong-Fil;Lim, Sung-Hun;Kim, Jae-Chul;Hyun, Ok-Bae
    • Proceedings of the KIEE Conference
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    • 2007.11b
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    • pp.180-181
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    • 2007
  • In this paper, SFCL using two magnetically coupled coils was modeled and simulated by PSCAD/EMTDC. The simulation was shown that fault current could be adjusted with the inductance ratio and the winding direction of two coils. The limited fault current in case of the additive polarity winding was lower than that of the subtractive polarity. The analysis results were compared with the calculated ones, and both the results agreed.

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Analysis on Current Limiting Characteristics According to the Influence of the Magnetic Flux for SFCL with Two Magnetic Paths

  • Ko, Seok-Cheol;Han, Tae-Hee;Lim, Sung-Hun
    • Journal of Electrical Engineering and Technology
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    • v.9 no.6
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    • pp.1909-1913
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    • 2014
  • In this study, a superconducting fault current limiter (SFCL) having two magnetic paths was proposed, and its current limiting characteristics were analyzed. For the SFCL to effectively perform the current limiting operation, it must be designed considering the magnetic saturation of the E-I core. Further, the influence of the magnetic flux on its peak current limiting characteristics was investigated. In addition, the magnetic flux curves of the SFCL obtained from the fault current limiting experiments were analyzed, and the subtractive polarity winding case was observed to not only further reduce the saturation potential of the core but also perform the peak current limiting functions well when compared with the additive polarity winding case.

Study on characterization of OTFT for patterned active layer P3HT using conventional photolithography (포토리소그래피를 이용한 P3HT 활성층의 패터닝에 의한 OTFT 특성 연구)

  • Park, Kyeong-Dong;Han, Kyo-Yong
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.11a
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    • pp.9-10
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    • 2006
  • The patterning for the active layer of organic semiconductors is important to attain completely organic-based OTFTs(Organic Thin Film Transistors). We studied on possibility of the application of the conventional photolithography technique to pattern the organic active layer poly(3-hexylthiophene)(P3HT). Patterned P3HT-based OTFTs with Bottom Contact(BC) configuration were fabricated using the conventional photolithography. We achieved field-effect mobilities in the saturation regime ${\sim}1.2{\times}10^{-3}cm^2/V{\cdot}s$, $I_{on/off}$ ratios ${\sim}10^5$ in the subtractive method, ${\sim}8{\times}10^{-4}cm^2/V{\cdot}s$, $I_{on/off}$ ratios ${\sim}10^3$ in the additive one.

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Fault Current limiting Characteristics of Flux-Lock type Superconducting Fault Current Limiter with Open-loop Iron Core according to the Voltage Level (개루프 철심을 이용한 자속구속형 초전도한류기의 전압별 전류제한 특성분석)

  • Nam, Gueng-Hyun;Choi, Hyo-Sang;Park, Hyoung-Min;Cho, Yong-Sun;Lee, Na-Young;Lim, Sung-Hun
    • Proceedings of the KIEE Conference
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    • 2005.11b
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    • pp.368-370
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    • 2005
  • Superconducting fault current limiter (SFCL) provides the effect such as enhancement in power system reliability due to limiting fault current in a few miliseconds. The flux-lock type SFCL among various type SFCLs consists of two coils wound on the same iron core and a component using the YBCO thin film. In the SFCL, operation characteristics can be controlled by adjusting the inductances and the winding directions of the coils. In this paper, we investigated the various fault current limiting characteristics according to the voltage level. To analyze the current limiting performance, we compared operational characteristics on the subtractive polarity winding direction on in case of open-loop iron core.

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Printed Polymer and a-Si TFT Backplanes for Flexible Displays

  • Street, R.A.;Wong, W.S.;Ready, S.E.;Chabinyc, M.L.;Arias, A.C.;Daniel, J.H.;Apte, R.B.;Salleo, A.;Lujan, R.;Ong, Beng;Wu, Yiliang
    • Journal of Information Display
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    • v.6 no.3
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    • pp.12-17
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    • 2005
  • The need for low cost, flexible, thin film transistor (TFT) display backplanes has focused attention on new processing techniques and materials. We report the development of TFT backplane technology based entirely on jet-printing, using a combination of additive and subtractive processing, to print active materials or etch masks. The technique eliminates the use of photolithography and has the potential to reduce the array manufacturing cost. The printing technique is demonstrated with both amorphous silicon and polymer semiconductor TFT arrays, and we show results of small prototype displays.

Application of Rapid prototyping for welding and milling, and Heat deformation for FEM (용접과 밀링을 이용한 쾌속조형법의 응용과 열변형 해석)

  • 류연화;최우천;송용억;박세형;조정권;신승환
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2000.11a
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    • pp.339-343
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    • 2000
  • Rapid prototyping for welding and milling is a hybrid approach that makes use of welding as additive and conventional milling as subtractive technique. For two years this concept has been used to verify manufacturing mold and mechanical parts successfully. In latest new fabrication methods. For example, manufacturing mold for two sort of materials and shell fabrication, have been applied to the concept in KIST. This methods will be an alternative proposal in rapid prototyping. Metal deposition for welding causes the part to deform. It is a handicap in our proceeding. To overcome this problem, in this paper, we represent an optimal welding path for FEM analysis. Eight paths are tried to this and the value of deformation is average and standard deviation in four points'. Then we can compare with eight cases and select the optimal path.

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Feature-Based Multi-Resolution Modeling of Solids Using History-Based Boolean Operations - Part II : Implementation Using a Non-Manifold Modeling System -

  • Lee Sang Hun;Lee Kyu-Yeul;Woo Yoonwhan;Lee Kang-Soo
    • Journal of Mechanical Science and Technology
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    • v.19 no.2
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    • pp.558-566
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    • 2005
  • We propose a feature-based multi-resolution representation of B-rep solid models using history-based Boolean operations based on the merge-and-select algorithm. Because union and subtraction are commutative in the history-based Boolean operations, the integrity of the models at various levels of detail (LOD) is guaranteed for the reordered features regardless of whether the features are subtractive or additive. The multi-resolution solid representation proposed in this paper includes a non-manifold topological merged-set model of all feature primitives as well as a feature-modeling tree reordered consistently with a given LOD criterion. As a result, a B-rep solid model for a given LOD can be provided quickly, because the boundary of the model is evaluated without any geometric calculation and extracted from the merged set by selecting the entities contributing to the LOD model shape.