• The Journal of the Society of Korean Medicine Diagnostics
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• v.15 no.2
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• pp.215-222
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• 2011

Objectives: The purpose of this study is to report the effect of Kamisamul-tang / Jia Wei Si Wu Tang / Augmented Four - Substance Decoction on a Menopausal Disorder Patient. This is a clinical report about a 49 year old female patient with a Menopausal Disorder treated by herb medicine, Kamisamul-tang / Jia Wei Si Wu Tang / Augmented Four-Substance Decoction without any other medicine or treatment. Methods: The patient was treated by herb medicine, Kamisamul-tang / Jia Wei Si Wu Tang / Augmented Four - Substance Decoction. The improvement of the patient was judged by Visual Analog Scale. Results The symptoms were gradually reduced and VAS became 1 after 8weeks of treatments, since then the symptoms were not reappearing for 3 months. Conclusions: Herb medicine, Kamisamul-tang / Jia Wei Si Wu Tang / Augmented Four - Substance Decoction is effective to improve a Menopausal Disorder Patient's symptoms. It is necessary to have more observation and many cases of patients with a Menopausal Disorder.

• Journal of Korean Society for Atmospheric Environment
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• v.16 no.4
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• pp.327-338
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• 2000

complexity in atmospheric environment coupled with shoreline and complex terrain often causes local variations of meteorology that are distinct from those representative over larger surrounding area, These kinds of local variations are less significant in usual long-term environmental impact analyses dealing with continuous plume. The variations could however be crucial in predicting dispersion of toxic substance released in a relatively small area for a short duration. In the present paper the effects of spatial and temporal resolution of diagnostic wind field on the dispersion of the released substance are investigated by using a puff model. A hypothetical release scenario assumes that a substance is released from a location in the Yochon Industrial Estate and passively dispersed within a few-kilometer distance for an hour. The results show that diagnostic analysis could resolve more spatial variations to some extent by employing smaller grid size. The peak concentrations and puff trajectories obtained from spatially -and/or tmeporally -varing diagnostic wind field are found appreciably different from those obtained from uniform wind field. Attention to high-resolution wind field in the both spatial and temporal spaces is called in the consequence analysis of toxic substance release.

• Journal of Microbiology and Biotechnology
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• v.30 no.1
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• pp.93-100
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• 2020

A bacterial strain inhibiting the growth of Vibrio anguillarum, the causative agent of vibriosis, was isolated from fish intestines. The isolated strain HS36 was identified as Aerococcus urinaeequi based on the characteristics of the genus according to Bergey's Manual of Systematic Bacteriology and by 16S rRNA sequencing. The growth rate and antibacterial activity of strain HS36 in shaking culture were higher than those in static culture, while the optimal pH and temperature for antibacterial activity were 7.0 and 30℃, respectively. The active antibacterial substance was purified from a culture broth of A. urinaeequi HS36 by Sephadex G-75 gel chromatography, Sephadex G-25 gel chromatography, and reverse-phase high-performance liquid chromatography. Its molecular weight, as estimated by Tricine SDS-polyacrylamide gel electrophoresis, was approximately 1,000 Da. The antibacterial substance produced by strain HS36 was stable after incubation for 1 h at 100℃. Although its antibacterial activity was optimal at pH 6-8, activity was retained at a pH range from 2 to 11. The purified antibacterial substance was inactivated by proteinase K, papain, and β-amylase treatment. The newly purified antibacterial substance, classified as a class II bacteriocin, inhibited the growth of Klebsiella pneumoniae, Salmonella enterica, and Vibrio alginolyticus.

• Fisheries and Aquatic Sciences
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• v.3 no.1
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• pp.52-63
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• 2000

Non-specific reaction has been a problem in doing, especially, research and diagnosis for infectious agents. Avidin-biotin-peroxidase complex (ABC) techniques has widely been used to amplify a reaction. Photobacterium damse1a subsp. piscicdia (formerly Pasteurella piscicida) exhibited a capacity to bind with streptavidin non-specifically. The band, estimated 26 K Da in Western blotted paper, was blocked with biotin but incompletely. In an attempt to explore an involvement of the non-specific substance in attaching piscine cells, cell attachment test performed using anti- Ph. d. subsp piscicida sera raised mouse and rabbit exhibited slightly blocking effects for Mediterranean (1736) and significantly for Japanese (Sp 92144) isolate. Biotin decreased the attachment ability significantly for Sp92144 but it was not effective to 1736. Both isolates showed greatly enhanced attachment ability with poly-L-lysin. The non-specific binding substance was contained in bacterial extracellular products (ECPs). The substance was able to purified with 2-imminobiotin affinity column, the purified substance appeared to have 4 bands in silver staining, and had a carbohydrate branch. This purified substance showed cytotoxic effects selectively between 5 piscine cell lines. Moreover, it stimulated rainbow trout macrophage in terms of reduction of cytochrome cas well as yeast phagocytosis, significantly.

• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.727-734
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• 1994

To elucidate the effect of calcitonin gene-related peptide(CGRP), vasoactive intestinal peptide(VIP) and substance P was investigated with perivascular nerve stimulation and treatment of peptides from polygraph in the isolated renal artery of rabbit. 1. The neurogenic contraction induced by perivascular nerve stimulation was the frequency-dependent manner(264 Hz) in the isolated renal artery of rabbit. 2. CGRP and VIP caused the relaxation on the precontraction with noradrenaline($10{\mu}m$) on the presence and absence of endothelium in the isolated renal artery of rabbit. 3. Substance P caused the endothelium-dependent relaxation on the precontraction with noradrenaline($10{\mu}m$) in the isolated renal artery of rabbit. 4. CGRP and VIP inhibited the neurogenic contraction by the perivascular nerve stimulation(0.3 ms, 80 V, 50 Hz, 1 sec) on the absence and presence of endothelium in the isolated renal artery of rabbit. 5. Substance P inhibited on the neurogenic contraction by the perivascular nerve stimulation with the endothelium-dependent in the isolated renal artery of rabbit.

• Biomolecules & Therapeutics
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• v.5 no.1
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• pp.94-99
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• 1997

Capsaicin is known to be an analgesic agent, affecting the synthesis, storage, , transport and release of substance p, the principal neurotransmitter of pain from periphery to the central nervous system(CNS). DA-5018, a newly synthesized capsaicin derivative has shown potent analgesic effect comparable to that of morphine in various rat models of experimentally inducted acute pairs. In this study the mechanism of analgesic actlvity of DA-5018 was examined. First, the electrically-evoked contraction of guinea pig trachea was inhibited by DA-5018 and these inhibition was recovered by incubation with capsafepine(3$\muM$), capsaicin receptor antagonist and this result suggested that DA-5018 has affinity on capsaicin receptor. The correlation between the norciceptive threshold and the release of substance P was evaluated. In vivo perfusion of slices of the rat spinal cord with DA-5018(10, 100$\muM$) produced a significant increase of the release of substance P and this increase was less than that of capsaicin(10$\muM$). The norciceptive threshold of rat treated with DA-5018(1 mg/kg, p.o) in tall pinch test increased from 2.9$\pm$0.3 to 23.5 $\pm$6.61. Tail pinch latency increased to a maximun at 15 min after DA-5018 treatment and then declined to control values by 120 min. The capsaicin-evoked release ot substance P from the spinal cord slices of rat treated with DA-5018 reduced from 2.38$\pm$ 0.79 to 0.69$\pm$ 0.26 pg/mg wet weight. This reduction reached to a minium at 15 min after DA-5018 treatment and then recovered to control value by 120 min. These results mean that analgesic activity of DA-5018 is due to release of substance P The effect of DA-5018 cream on electrically-evoked neurogenic inflammation of rat saphenous nerve was compared with capsaicin (zostrix-HP). DA-5018 showed 34% inhibition of the neurogenic extravasation while capsaicin showed significant 67% inhibition. This result indicates that the potency of DA-5018 in the release of substance P is less than that of capsaicin. These results suggest that the release of substance P is partially involved in the mechanism of analgesic action of DA-50l8.

• Journal of Oriental Neuropsychiatry
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• v.9 no.1
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• pp.73-82
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• 1998

Substantial evidence has accumulated that Alzheimer's disease is associated with a local inflammatory reaction in senile plaques which may be immunemediated, and includes extensive Brain Neuroglial invasion, lymphocytic infiltration, cytokine deposition. Tumor necrosis factor a (TNF-a) is a cytokine which plays an important immunoenhancing role in the local acute and chronic inflammatory response in response to a variety of stimuli. The neuropeptide, substance P, can stimulate secretion of TNF-a from Brain Neuroglial cells. Neuroglia have substance P receptors in the central nervous system. WQ investigated whether RADIX ASPARAGI inhibits secretion of TNF-a from primary cultures of Brain Neuroglial cells containing both astrocyte (∼90%) and microglia (∼10%). RADIX ASPARAGI dose-dependently inhibited the TNF-a secretion induced by substance P plus lipopolysaccharide (LPS). In cultures enriched for micoglia (>95% pure). LPS stimulated the secretion of TNF-a but substance P caused no enhancement. Because there was no synergism between substance P and LPS in the microglial cultures it is resonable to substance P madiated enhancement of TNF-a secretion. IL-1 is a modulator of TNF-a secretion in the immune system. Also IL-1 has been shown to elevate TNF- a secretion from LPS-stimulated Brain Neuroglial cells while having no effect on Brain Neuroglial cells in the absence of LPS. We therfore investigated whether IL-1 mediates the RADIX ASPARAGI inhibition of TNF-a secretion form primary Brain Neuroglial cells. Treatment of RADIX ASPARAGI to mixed cultures stimulated with both substance P and LPS decreased TNF-a secretion to the level observed with LPS alone. These results indicate that RADIX ASPARAGI possess strong antiinflammatory activity in the cental nervous system by inhibition of inflammatory cytokines secretion from Brain Neuroglial cells.

• Fire Science and Engineering
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• v.28 no.6
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• pp.90-98
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• 2014

Lately accidents of harmful chemical substance in korea were repeatedly replaying. We studied problems and preparation plan in 2008 years~2014 years through analysis cases of harmful chemical substance. Leakage of chemical substance's accident situation shows that leakage in the workplace is the most 10 cases, accounting for 58.6% is the largest proportion in 2009. Leakage according to explosion accounting for 50% (5 cases) in 2004, 25% (4 cases) in 2007 and 33.3% (4 cases) in 2011 has very irregular change. Therefore, A major cause of accidents is lack of safety management. Harmful chemical substance showed various aspects, high residual substance and non-visible characteristic so response and recovery was easy. And the second damage to move a distance causes environment pollution. So a mutual-assistance system and integrated system of related organization have to build and expert of chemical incident response and therapist of chemical substance response have to establish to do efficient and rapid response.

• Child Health Nursing Research
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• v.9 no.4
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• pp.432-440
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• 2003

Purpose: To provide basic data for strategy of substance abuse through the investigation of the status of substance abuse and knowledge and attitude about drugs in the elementary students. Method: Subjects were 565 6th grade of elementary students. Data were collected from June to July 2001 by using structured questionnaire. Result: 1) Among the students of respondents, 10.2% of them had the experience in smoking, 26.7% and 08% of them in drinking and inhalants, respectively. 2) The degree of students' knowledge about substance was 20.9 points on average with the highest score being 30. Students' knowledge about substances, smoking came on top, next came drinking and inhalants followed them. There were no significant differences in the knowledge of substances by general and drug related characteristics of the students. 3) The students' attitude about substances was negative with 30 points on average with the highest score being 108. As for the attitude about substances, they revealed the most generous in drinking next in smoking and inhalants. Those of boys, bored in school life and experienced in substance abuse were significantly generous in the attitude about substances. 4) There was a significant weak inverse relationship between the student's knowledge and attitude about substances (r=-0.13). As for the relationship between the student's knowledge and attitude about substances, the strongest relationship was shown in inhalants (r=-0.18) next in drinking (r=-0.16) and smoking (r=-0.06). Conclusion: The results of the finding suggest that the developing of programs in substance abuse for the elementary students are needed not only for the prevention but also for the cessation.

• Microbiology and Biotechnology Letters
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• v.3 no.2
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• pp.69-72
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• 1975

Isolation of the toxic substance which was produced by Streptentyces sp. and it's biorhemical characterestics and toxicity to fishes were reported in the previous paper. The present report includes antibiotic activity of the substance, inhibitory activity of the substance on the succinic dehydrogenase of fishes, and its effect on the blood corpuscles of a rabbit. An evident antibiotic activity of this substance was observed on Candida yeasts, but n$ot^1$on molds or bacteria. The substance inhibited the growth of Candida japonica and C. utilis to the 50％ at the concentrations of 7.5 and 10.2ug per ml, respectively. The activity of succinic dehydrogenase obtained from various organs of Cyprinous carpio L. was also found to be inhibited by this substance. Original activities of the enzymes from tht brain, kidney, and liver, were inhibited by 75.4％, 38.2％, and 26.2％, respectively, but not the enzymes from the heart and spleen. Neither leukopenia nor leukocytosis was detected after the intravenous administration of the substince to the rabbit at the level of 6 mg per 2 kg body weight.